臺灣博碩士論文加值系統

在精緻化飲食的現代，肥胖是引起了許多健康問題的主要原因。因此，罹患糖尿病的風險也大大增加，而糖尿病（Diabetes mellitus，DM）起因於長期胰島素阻抗而引發高血糖；因而影響男性生殖功能，是生育率低的關鍵問題之一。是因為引起慢性發炎和氧化壓力，從而導致男性生殖功能障礙，損害睾丸和精子，干擾精子的生長，導致不育。
本研究採用的雞肝水解物，是由屠宰白肉雞時，通常是廢棄的雞肝下腳料製成的。製程是用胃蛋白酶分解後，過濾雜質，再進行冷凍乾燥成粉末。
檢視相關文獻指出，雞肝水解物具有抗氧化、抗肥胖、抗肝纖維化、抗心臟損傷和抗腎功能等功用。因此，本實驗處理雞肝水解物，對細胞實驗採用生殖細胞實驗，觀察誘導發炎模式，以測試抗發炎的效果。
動物實驗步驟是先採用高脂飲食（HFD）誘導肥胖，再給予低劑量Streptozotocin（35 mg/kg）誘導雄性大鼠成為第二種糖尿病模型。實驗組分為:對照組、糖尿病組、陽性對照組（Metformin，200 mg/kg）。以常見的糖尿病藥物、雞肝水解物低劑量組（240 mg/kg）、中劑量組（480 mg/kg）和高劑量組(1200 mg/kg) ，繼續管餵六週。
實驗結果顯示，在中劑量組（480 mg/kg）具有顯著的抗糖尿病和改善賀爾蒙平衡作用；血漿中睪固酮水準有明顯的上升，且提升精子總數和泳動率改善生殖功能。本實驗結果驗證，食用雞肝水解物可以增加血中睪固銅，並改善賀爾蒙調節。

In the modern era of refined diet, obesity has caused many health problems. Among them, the risk of diabetes is greatly increased, and diabetes (Diabetes mellitus, DM) can cause the problem of low fertility due to long-term insulin resistance and high blood sugar. It can cause chronic inflammation and oxidative stress, which can cause male reproductive dysfunction, damage testicles and sperm, interfere with spermatogenesis, and cause infertility.
Chicken liver hydrolysate is made from leftover chicken liver scraps left over from the slaughter of white broilers. It is use pepsin to digest and then freeze-dried into powder. It has anti-oxidation, anti-obesity, anti-liver fibrosis, anti-heart damage, and anti-kidney properties. Function damage and other effects. Therefore, this experiment chooses to use chicken liver hydrolysate, cell experiment uses germ cell experiment to observe the effect of inflammation mode, animal experiment uses high fat diet (HFD) and low dose Streptozotocin (35 mg/ kg) induced male rats to become the second diabetes model. The experimental groups were divided into control group, diabetes group, positive control group (Metformin, 200 mg/kg), chicken liver hydrolysate low-dose group (240 mg/kg), medium The dose group (480 mg/kg) and the high-dose group (1200 mg/kg) continued to feed for six weeks. The experimental result is that the middle-dose group (480 mg/kg) has a significant effect on anti-diabetics and improving hormone balance.

目錄
摘要 I
Abstract II
目錄 III
名詞縮寫表 VIII
壹、 緒論 1
貳、 文獻回顧 3
一、 男性生殖與不孕症 3
1.1 男性不孕症之定義 3
1.2 男性不孕症之診斷 3
1.3 男性不孕症之成因 3
1.4 男性生殖內分泌調控 5
二、 糖尿病 6
2.1 糖尿病前期(Prediabetes) 6
2.2 糖尿病之診斷標準 6
2.3 糖尿病之分類 7
三、 糖尿病與男性生殖 8
3.1 糖尿病與氧化壓力 8
3.2 糖尿病與生殖內分泌 9
3.3 動物誘導糖尿病模式探討 9
3.4 糖尿病與藥物Metformin 9
四、 白肉雞 10
4.1 白肉雞之簡介 10
4.2 雞肝之成分分析 10
4.3 雞肝水解物之成分分析 10
4.4 雞肝水解物之生理活性 11
一、 細胞實驗 13
二、 動物實驗 14
三、 大鼠犧牲後分析項目 15
參、 實驗材料與方法 16
一、 實驗材料 16
1.1 雞肝水解物 16
1.2 細胞株 16
1.3 實驗動物 16
1.5 藥品 18
1.6 儀器設備 21
二、 細胞實驗 22
2.1細胞培養 22
2.2細胞存活率測定 (MTT assay) 22
肆、 結果 32
一、 細胞實驗 32
1.1 H2O2 誘導LC-540細胞氧化壓力模式 32
二、 動物實驗 33
2.1 CLH對於糖尿病大鼠血糖及胰島素含量之影響 33
2.2 CLH 對於糖尿病大鼠臟器重量百分比之影響 33
2.3 CLH對於糖尿病大鼠體內氧化壓力之影響 33
2.4 CLH對於糖尿病大鼠體內發炎反應之影響 34
2.5 CLH對於糖尿病大鼠體內生殖系統之影響 34
2.6 CLH對於糖尿病大鼠生殖內分泌蛋白表現量之影響 35
伍、 討論 37
陸、 結論 40
柒、 參考文獻 41
捌、 圖表 48
玖、 附錄 63

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