臺灣博碩士論文加值系統

摘要 I
ABSTRACT II
目錄 IV
表目錄 VII
圖目錄 IX
第1章 緒論 1
1.1 前言 1
1.2 研究動機與目的 2
1.3 論文研究架構 3
第2章 文獻回顧 5
2.1 超臨界流體萃取 5
2.1.1 超臨界流體介紹 5
2.1.2 超臨界流體性質 6
2.1.3 影響超臨界流體萃取效果之因素 6
2.1.4 超臨界流體萃取模式 8
2.1.5 超臨界流體萃取之現況 9
2.2 萬壽菊及葉黃素之簡介 10
2.2.1 葉黃素簡介及現況..………………………………………………………...11
2.3 使用HPLC測量萬壽菊萃取物之化學成分 12
2.4 自由基和抗氧化成分及機制 14
2.4.1 抗氧化機制 15
2.5 CENTRAL COMPOSITE DESIGN實驗設計法 18
2.6 常見的天然萃取方法 19
第3章 實驗藥品材料 20
3.1 實驗藥品材料 20
3.1.1 材料 20
3.1.2 藥品 20
3.2 實驗設備及方法 24
3.2.1 超臨界流體萃取實驗設備 24
3.2.2 儀器設備 27
3.2.3 實驗方法 30
3.2.3.1 高效能液相層析儀(HPLC) 30
3.2.3.2 紫外光與可見光光譜儀(UV-Vis) 32
• 抗氧化活性測試 32
• 總酚含量測定 39
3.2.3.3 傅立葉轉換紅外線光譜儀(FTIR) 41
3.2.3.4 氣相層析儀(GC-MS) 43
3.3 實驗流程 44
3.3.1 萃取參數確認 44
3.3.2 實驗設計法分析 44
3.3.3 檢測分析 45
第4章 結果與討論 48
4.1 萬壽菊鮮花前處理 48
4.2 萃取平衡時間 52
4.3 迴歸模式之選擇 53
4.4 變異數分析 55
4.4.1 萬壽菊萃取物產率變異數分析 55
4.4.1.1 Linear、2FI和Quadratic回歸模式分析 58
4.4.1.2 反應曲面分析 63
4.4.2 葉黃素總含量變異數分析 65
4.4.2.1 Linear、2FI和Quadratic回歸模式分析 68
4.4.2.2 反應曲面分析 73
4.5 HPLC檢測測定 75
4.6 抗氧化活性測試 76
4.7 總酚含量測試 76
4.8 萬壽菊萃取物定性分析 71
4.8.1 FTIR檢測分析 71
4.8.2 HPLC檢測分析 78
4.8.3 GC-MS檢測分析 93
第5章 結論 100
參考文獻 101
符號彙編 107
附錄 108
一、超臨界萃取實驗步驟 二、高效能液相質譜儀裝置步驟

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