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研究生:張娓菁
研究生(外文):JHANG, WEI-JING
論文名稱:混和陰/陽離子界面活性劑對包覆芹菜素苦茶油/可可脂奈米載體物化性質的影響
論文名稱(外文):Effects of Mixed Anionic/Cationic Surfactants on Physicochemical Properties of Camellia oil/Cocoa butter Nanocarriers for Apigenin Encapsulation
指導教授:周宗翰
指導教授(外文):CHOU, TZUNG-HAN
口試委員:周宗翰張艦祥郭勇志楊宏達
口試委員(外文):CHOU, TZUNG-HANCHANG, CHIEN-HSIANGKUO, YUNG-CHIHYANG, HONG-TA
口試日期:2022-06-28
學位類別:碩士
校院名稱:國立雲林科技大學
系所名稱:化學工程與材料工程系
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:2022
畢業學年度:110
語文別:中文
論文頁數:83
中文關鍵詞:芹菜素脂質奈米載體混合界面活性劑陰/陽離子界面活性劑抗氧化性藥物包覆
外文關鍵詞:ApigeninNanocarriers based on lipidMixed surfactantAnionic/Cationic SurfactantsAntioxidantDrug Encapsulation
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  • 被引用被引用:0
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  • 收藏至我的研究室書目清單書目收藏:0
芹菜素為一種具有生物活性的黃酮類化合物,存在於多種植物、蔬菜、功能性食品及藥用植物中,且芹菜素具有多種對人體有益的特性,如抗氧化、抗癌及抗炎等功能。然而,芹菜素的低水溶性及低生物利用度使其在治療應用上受到限制。為此,我們開發了混合具有功能性的陰陽離子界面活性劑以製備基於脂質奈米載體,以用此提高芹菜度之生物可利用度。在本實驗中,以苦茶油(CO)及可可脂(CB)作為脂質核心,然後混合陰離子界面活性劑(Surfactant T)/陽離子界面活性劑(十六烷基三甲基溴化銨, CTAB),通過高速均質機-超聲波震盪法製備脂質奈米載體。研究了混合Surfactant T /CTAB對於奈米載體的粒徑、多分散性、界面電位、相變行為及奈米載體內流動性的影響。同時,利用高效能液相層析儀分析奈米載體對於芹菜素的包覆率及負載率,並利用可見光光譜儀研究其化學穩定性。研究結果顯示,固定於油含量1%和2%的CO/CB奈米載體平均粒徑皆小於350 nm,多分散係數為0.15~0.3,界面電位絕對值隨者ST比例增加有上升的趨勢。CO/CB奈米載體的熱焓量隨著ST比例增而下降。CO/CB奈米載體內流動性結果表示在摩耳比ST:SC=2:1時,流動性最低;而在摩耳比ST:SC=1:1時,流動性最高。CO/CB奈米載體對於芹菜素之包覆率大於90%,負載率約在0.3%。此外,APG-CO/CB奈米載體具有良好的化學穩定性,且可以有效保護芹菜素不被氧化。
Apigenin, as a bioactive flavonoid compound present in a variety of vegetables, fruit, functional foods, and medicinal plants exhibits a wide range of health beneficial properties such as antioxidant, anticancer, and anti-inflammatory; however, its therapeutic application was limited because of its poor water solubility and low bioavailability. For this, We developed of mixed anionic/cationic surfactants with bio-activity to prepare the nanocarriers based on lipid to enhance the bioavailability of apigenin. In this study, we prepared nanocarriers to use Camellia oil (CO) and Cocoa butter (CB) as lipid core and then mixed anionic (Surfactant T)/cationic ( Hexadecyl trimethyl ammonium bromide, CTAB) surfactants through a homogenization-ultrasonication method. The effect of different surfactants on particle size, polydispersity, zeta potential, phase transition behavior, and the intra-particle fluidity of nanocarriers were investigated. Meanwhile, the encapsulation efficiency and loading efficiency for apigenin were investigated by high-performance liquid chromatography and the chemical stability was investigated by UV-visible. Results showed all CO/CB nanocarriers with fixed oil content 1% and oil content 2% had average particle size less than 350 nm, polydispersity 0.15 to 0.30, and the absolute value of zeta potential increased as the molar ratio of ST increasing. The phase transition enthalpy of CO/CB nanocarriers decreased with increasing the molar ratio of ST. The intra-particle fluidity of CO/CB nanoparticles results showed the molar ratio of ST:SC=2:1 has a lower fluidity and the molar ratio of ST:SC=1:1 has a higher fluidity. The apigenin encapsulation efficiency of CO/CB nanocarriers was more than 90% and loading efficiency was approximate 0.30%. Besides, the chemical stability of APG-CO/CB nanocarriers had great chemical stability and can effectively protect apigenin from oxidization.
摘要 i
Abstract ii
目錄 iii
表目錄 iv
圖目錄 vii
符號說明 x
第一章 緒論 1
1-1 前言 1
1-2 研究目的與動機 2
第二章 文獻回顧 3
2-1 脂質奈米載體 3
2-1-1 奈米乳液 3
2-1-2 固體脂質奈米粒子 5
2-1-3 奈米結構脂質載體 5
2-2 苦茶油 6
2-3 可可脂 7
2-4 陰陽離子奈米系統 8
2-4-1 陰離子型界面活性劑-界劑T 9
2-4-2 陽離子型界面活性劑-十六烷基三甲基溴化銨 9
2-5 芹菜素 10
第三章 材料與方法 11
3-1 實驗藥品 11
3-2 實驗設計與方法 12
3-2-1 奈米載體配方設計 12
3-2-2 奈米載體之製備流程 12
3-2-3平均粒徑、多分散係數與界面電位之分析 13
3-2-4 實驗設計軟體分析 13
3-2-5 穿透式電子顯微鏡分析 13
3-2-6 微分熱掃描卡計分析 14
3-2-7 螢光偏極化分析 14
3-2-8 奈米載體包覆芹菜素之測定 14
3-2-9 奈米載體包覆芹菜素之化學穩定性分析 15
第四章 結果與討論 22
4-1 苦茶油/可可脂混和陰陽離子界面活性劑界劑T/CTAB奈米載體之物性分析 22
4-1-1 奈米載體之粒徑、多分散係數、界面電位及穩定天數 22
4-1-2 奈米載體之反應曲面分析 24
4-1-3 奈米粒子之型態分析 25
4-1-4 奈米載體之相變行為分析 25
4-1-5 奈米載體之粒子內流動性分析 27
4-2苦茶油/可可脂奈米載體包覆芹菜素 28
4-2-1 苦茶油/可可脂奈米載體包覆芹菜素之粒徑、多分散係數、界面電位及包覆效率 28
4-2-2 苦茶油/可可脂奈米載體包覆芹菜素之化學穩定性之研究 30
第五章 結論 66
參考文獻 67




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