臺灣博碩士論文加值系統

光醱酵生物產氫（photo-fermentative biohydrogen production，PFHP）的過程是通過光合細菌的固氮酶進行光分解有機物來產生氫氣，但在固氮酶催化反應的過程中高能量需求以及生物製產氫的產氫效率已成為光醱酵大規模應用的首要問題。本研究主要目的為優化以 Rhodopseudomonas palustris WP3-5 為主菌株的光醱酵產氫的表現。實驗原理為光合菌以葡萄糖作為碳源進行產氫，添加不同濃度的鐵離子 (100~900 mg-Fe/L)、鉬離子 (1~100 mg-Mo/L)、鎳離子 (1~100 mg-Ni/L) 以及鋅離子 (10~900 mg-Zn/L) 來觀察金屬離子對於 PFHP 的影響。另外添加不同濃度的活性碳顆粒 (100~900 mg-GAC/L) 以及活性炭粉末 (1~700 mg-PAC/L) 來觀察碳顆粒對於 PFHP 的影響。因此本研究皆使用氫氣產率作為評估指標。
在批次系統中研究了許多參數，例如基質的碳氮比 (C/N ratio)、pH、金屬離子與碳顆粒的添加量等。實驗結果表明，基質控制在碳氮比 25 以及 pH 7.5 的情況下有較高的氫氣產率。另外，添加 5 mg-Mo/L 其氫氣產率為 2.685 mol H2/mol glucose，與對照組相比提升 63.4% 的產氫速率；添加 10 mg-PAC/L 其氫氣產率為 2.412 mol H2/mol glucose，與對照組相比提升 62.7% 的產氫速率。
由上述實驗結果可知，添加金屬離子以及碳顆粒等輔助因子能夠有效提升氫氣產率，尤其添加鉬離子最為顯著，對於光醱酵大規模應用有著至關重要的作用。

The photo-fermentative biohydrogen production (PFHP) involves the hydrogen production through the light-induced decomposition of organic compounds by photosynthetic bacteria's nitrogenase. However, the high energy demand and low hydrogen production efficiency of the nitrogenase catalytic reaction have become the primary challenges in the large-scale application of photo-fermentation. The main objective of this study is to optimize the performance of photo-fermentative biohydrogen production using Rhodopseudomonas palustris WP3-5 as the predominant strain. In the experimental setup, photoautotrophic bacteria utilize glucose as a carbon source for hydrogen production, and the addition of different concentrations of metal ions such as iron ions (100-900 mg-Fe/L), molybdenum ions (1-100 mg-Mo/L), nickel ions (1-100 mg-Ni/L), and zinc ions (10-900 mg-Zn/L) were work out to observe the influence of metal ions on PFHP. Additionally, the addition of various concentrations of granular activated carbon (100-900 mg-GAC/L) and powdered activated carbon (1-700 mg-PAC/L) examined the effect of carbon particles on PFHP. Therefore, the hydrogen production rate used as the evaluation parameter.
Several parameters, including the C/N ratio of the substrate, pH, and the amount of metal ions and carbon particles added, are investigated in batch systems. The experimental results indicate that maintaining a carbon-to-nitrogen ratio of 25 and a pH of 7.5 resulted in higher hydrogen production rates. Furthermore, adding 5 mg-Mo/L leads to a hydrogen production rate of 2.685 mol H2/mol glucose, representing a 63.4% increase compared to the control group. Similarly, adding 10 mg-PAC/L resulted in a hydrogen production rate of 2.412 mol H2/mol glucose, exhibiting a 62.7% improvement compared to the control group.
Based on the above experimental results, it is evident that adding of metal ions and carbon particles as auxiliary factors can effectively enhance the hydrogen production rate in photo-fermentation. Obviously, adding molybdenum ions demonstrates significant effects and holds critical importance for the large-scale application of photo-fermentation.

第一章 緒論 1
1-1 前言 1
1-2研究動機和目的 2
1-3實驗架構 2
第二章 文獻回顧 4
2-1 生質產氫 4
2-1-1 產氫方式 5
2-1-2 光醱酵反應產氫 7
2-1-3 光醱酵產氫代謝路徑 9
2-2 微生物動力學建模 10
2-2-1 微生物生長 12
2-2-2 微生物產氫 14
2-3 光醱酵厭氧產氫之變因 16
2-3-1 pH 變因 17
2-3-2 溫度變因 18
2-3-3 光照變因 19
2-3-4 基質種類與濃度 19
2-3-5 金屬離子 20
2-3-6 碳顆粒 21
2-4 微生物定量 22
第三章 實驗方法 23
3-1 菌體來源 23
3-2 營養配方來源 23
3-3 實驗儀器與裝置 24
3-4 實驗方法與步驟 25
3-5 實驗與藥品分析 26
3-5-1 實驗藥品 26
3-5-2 氣相組成分析 27
3-5-3 液相組成分析 28
3-5-4 菌重分析 28
3-5-5 菌項分析 29
第四章 結果與討論 30
4-1 在不同 PH 值的環境對光醱酵產氫之影響 31
4-2 添加不同碳氮比的營養源對光醱酵產氫之影響 35
4-3 菌項分析 37
4-4 添加不同微量元素對光醱酵產氫之影響 39
4-4-1 鐵離子 41
4-4-2 鉬離子 43
4-4-3 鋅離子 45
4-4-4 鎳離子 47
4-5 添加活性碳對光醱酵產氫之影響 51
4-6 活性碳粉末對透光度之影響 56
第五章 結論 57
參考文獻 58

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