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研究生:蕭楚霖
研究生(外文):Hsiao, Chu-Lin
論文名稱:中藥百合的外來物代謝交互作用具有組織選擇性
論文名稱(外文):The tissue-selective xenobiotic interactions with Lilii bulbus
指導教授:翁芸芳蕭崇瑋
指導教授(外文):Ueng, Yune-FangShiau, Chung-Wai
口試委員:翁芸芳蕭崇瑋陳瑞明
口試委員(外文):Ueng, Yune-FangShiau, Chung-WaiChen, Ruei-Ming
口試日期:2023-07-27
學位類別:碩士
校院名稱:國立陽明交通大學
系所名稱:生物藥學研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2023
畢業學年度:111
語文別:中文
論文頁數:61
中文關鍵詞:百合鱗莖CYP1regaloside Aregaloside BAHRAHRR
外文關鍵詞:Lilii bulbus (LB)CYP1regaloside Aregaloside BAHRAHRR
相關次數:
  • 被引用被引用:0
  • 點閱點閱:27
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誌謝 i
中文摘要 ii
英文摘要 iv
目錄 vi
圖目錄 viii
表目錄 xi
第一章 緒論 - 1 -
1.1 Xenobiotic metabolism - 1 -
1.2 Cytochrome P450 (CYP) - 1 -
1.3 CYP1 family - 2 -
1.4 Phase II metabolism - 3 -
1.5 Lilii bulbus - 3 -
第二章 實驗材料及方法 - 6 -
2.1 化學藥物及溶劑 - 6 -
2.2實驗動物處理及微粒體 (microsome) 及細胞質液 (cytosol) 製備 - 6 -
2.3採血 - 7 -
2.4 肝、腎功能血液檢測 - 7 -
2.5 蛋白質含量測定 - 7 -
2.6單氧酶活性測定 - 8 -
2.6.1 CYP 含量及NADPH-CYP reductase活性測定 - 8 -
2.6.2 Cytochrome b5 含量測定 - 8 -
2.6.3 7-Ethoxyresorufin O-deethylation (EROD) 活性測定及7-methoxyresorufin O-demethylation (MROD) 活性測定 - 8 -
2.6.4 7-Pentoxyresorufin O-deallkylation (PROD) 活性測定 - 9 -
2.6.5 Dextromethorphan O-demethylation (DMOD) 活性測定 - 9 -
2.6.6 Coumarin hydroxylation 活性測定 - 9 -
2.6.7 Nifedipine oxidation (NFO) 活性測定 - 10 -
2.6.8 Chlorzoxazone hydroxylation 活性測定 - 10 -
2.6.9 Warfarin hydroxylation 活性測定 - 10 -
2.7 Uridine 5’-diphosphate-glucuronosyltransferase (UGT) 活性測定 - 11 -
2.8 Glutathione S-transferase (GST) 活性測定 - 11 -
2.9 NAD(P)H:quinone oxidoreductase (NQO) 活性測定 - 11 -
2.10 Immunoblot analyses免疫墨點法分析 - 11 -
2.11 Cell Culture 細胞培養 - 12 -
2.12 MTT assay 細胞毒性測定 - 12 -
2.13 Total RNA isolation (總核糖核酸之分離) - 12 -
2.14 Reverse transcription (反轉錄) - 13 -
2.15 Real- time polymerase chain reaction (即時聚合酶連鎖反應) - 13 -
2.16實驗儀器 - 15 -
2.17實驗分析 - 15 -
第三章 實驗結果 - 16 -
3.1 百合的成分分析 - 16 -
3.2 百合對鼷鼠的體重及肝腎毒性分析 - 16 -
3.3 百合對鼷鼠體內代謝酶的劑量相關性 - 17 -
3.4 百合成分對肝、肺細胞株之存活率 - 18 -
3.5 百合成分對CYP1的誘導影響 - 18 -
第四章 討論 - 20 -
參考文獻 - 24 -
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