臺灣博碩士論文加值系統

芝麻葉 （Arugula） 屬十字花科的蔬菜，營養價值極高，具有葉黃素、β-胡蘿蔔素、維生素A、維生素C、維生素K、葉酸和多種礦物質以及膳食纖維的天然來源。芝麻葉具有強化身體解毒機能的硫代葡萄糖苷，也富含相當高的抗氧化活性，能夠提高人體ORAC（Oxygen radical absorbance capacity）氧化自由基清除能力。光合細菌廣泛分佈於水田、河川、海洋和土壤中的一大類微生物，為革蘭氏陰性細菌。近年來應用在水產養殖業及農業上，以微生物作為植物肥料，可幫助植物吸收養分以及維持健康並且能減少農化產品的使用，因此也被視為一種對生態環境友善的施肥方式。本研究目的旨在評估透過水耕栽培方式添加光合菌微生物hodobacter sphaeroides（ATCC17023） 作為生物性肥料對十字花科的裂葉芝麻葉產量 （鮮乾重）、營養素含量、抗氧化能力之影響。根據實驗結果顯示，在水耕芝麻葉中，光合細菌的共培養不僅提升芝麻葉產量 （p<0.05），且相較於未添加光合菌的組別顯著增加其根重、生長重量、地上重和葉片數 （p<0.05）。在DPPH自由基清除能力試驗中，芝麻葉水萃物在1.25、2.5、5μg/mL的濃度下，皆可顯著增加的自由基清除能力，隨著劑量的提高抗氧化活性呈現上升的趨勢，且光合菌添加組效果顯著優於未添加組 （p<0.05）。此外，在總抗氧化能力TEAC試驗中，光合菌與芝麻葉的共培養亦顯著增加芝麻葉的總抗氧化能力 （p<0.05）。綜合以上結果得知，R.sphaeroides光合菌潛力菌株可利用做為水耕栽培系統上的微生物肥料，達到增加作物產量與增加抗氧化活性之效果。

Sesame leaves (Arugula), belonging to the Brassicaceae family, are highly nutritious vegetables rich in lutein, β-carotene, vitamin A, vitamin C, vitamin K, folic acid, various minerals, and dietary fiber. Sesame leaves contain glucosinolates that enhance the body's detoxification capabilities and are also high in antioxidant activity, contributing to improved ORAC (oxygen radical absorbance capacity) in the human body. Photoautotrophic bacteria, a widespread type of microorganism found in paddy fields, rivers, oceans, and soil, are Gram-negative bacteria. In recent years, they have been applied in aquaculture and agriculture as microbial fertilizers to help plants absorb nutrients, maintain health, and reduce the use of chemical products, making them an environmentally friendly fertilization method.
This study aims to assess the impact of adding the photosynthetic bacterium Rhodobacter sphaeroides (ATCC17023) through hydroponic cultivation as a biofertilizer on the yield (fresh and dry weight), nutrient content, and antioxidant capacity of Brassicaceae, specifically split-leaved sesame leaves. The experimental results indicate that co-cultivation with photoautotrophic bacteria not only significantly increases the yield of sesame leaves in hydroponics (p<0.05) but also significantly increases root weight, total weight, above-ground weight, and leaf number compared to the group without the addition of photoautotrophic bacteria (p<0.05).In the DPPH free radical scavenging test, sesame leaf extracts at concentrations of 1.25, 2.5, and 5μg/mL significantly increased free radical scavenging capacity, showing an upward trend in antioxidant activity with increasing dosage. Moreover, the group with added photoautotrophic bacteria exhibited significantly better results than the group without (p<0.05). Additionally, in the total antioxidant capacity TEAC test, co-cultivation of photoautotrophic bacteria with sesame leaves also significantly increased the total antioxidant capacity of sesame leaves (p<0.05).In summary, the photosynthetic bacterium strain ATCC17023 shows potential as a microbial fertilizer in hydroponic cultivation systems, leading to increased crop yield and enhanced antioxidant activity.

誌謝 i
摘 要 iv
Abstract v
目 錄 vii
表 目 錄 viii
圖 目 錄 ix
第一章 緒論 1
第一節 研究背景 1
第二節 研究動機 2
第三節 研究目的 4
第四節 研究方法與研究假說 6
第二章 文獻探討 7
第一節 光合菌-球形紅桿菌（Rhodobacter sphaeroides） 7
第二節 微生物肥料 10
第三節 水耕栽培與水耕蔬菜 12
第四節 抗氧化能力 13
第五節 芝麻葉營養素 14
第三章 研究方法 18
第一節 研究流程 18
第二節 研究架構與假說 21
第三節 實驗設計與嚴謹度 22
第四章 材料與方法 25
第一節 實驗材料與儀器設備 25
第二節 實驗方法 30
第三節 資料分析方法 36
第五章 結果 44
第一節 實驗植株每日PH、EC、溫度監測統計 44
第二節 光合菌對水耕芝麻葉之葉片數產量之影響 50
第三節 光合菌對水耕芝麻葉之生長重量之影響 52
第四節 光合菌對水耕芝麻葉之地上重/根重產量之影響 54
第五節 水耕芝麻葉採收後總產量分析 56
第六節 光合菌對水耕芝麻葉自由基清除能力之效果 57
第七節 光合菌對水耕芝麻葉不總抗氧化能力之效果 59
第八節 水耕芝麻葉之營養素含量分析 61
第六章 討論 66
第七章 結論 71
第八章 參考文獻 72

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