跳到主要內容

臺灣博碩士論文加值系統

(44.211.31.134) 您好!臺灣時間:2024/07/24 18:39
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:劉玉倩
研究生(外文):LIU, YU-CHIEN
論文名稱:以霧化方式導入丹參植萃複方對皮膚淡斑成效之評估
論文名稱(外文):Induction of extraction of Salvia miltiorrhiza for evaluation of the improving mechanism of skin spot using atomization method
指導教授:楊堉麟楊堉麟引用關係
指導教授(外文):YANG,YU-LIN
口試委員:王耀賢陳建安
口試委員(外文):WANG, YAO-HSIENCHEN, CHIEN- AN
口試日期:2023-12-26
學位類別:碩士
校院名稱:中華醫事科技大學
系所名稱:生物醫學研究所
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2024
畢業學年度:112
語文別:中文
論文頁數:57
中文關鍵詞:丹參抗氧化美白黑色素霧化
外文關鍵詞:Salvia miltiorrhizaantioxidantwhiteningmelaninatomization
相關次數:
  • 被引用被引用:0
  • 點閱點閱:58
  • 評分評分:
  • 下載下載:12
  • 收藏至我的研究室書目清單書目收藏:0
背景
自古以來,愛美是人的天性,隨著時代的變遷,現代人追求美的方式從內在到外在,內在要求養生;各式美白抗老保健食品因應而生,外在更要求立竿見影的效果;醫學美容為了達到各方的需求,各式各樣的快速護膚雷射一應俱全。因此舉凡可以外敷內服的美白抗氧化成分,成了不老代名詞,大家趨之若鶩。
丹參是歷史悠久的中草藥,屬於脣形科。有親脂性成分(丹參酮 I、丹參酮 IIa、丹參酮 IIb、隱丹參酮、二氫丹蔘酮等)和親水性成分(丹參素、丹酚酸 A 和 B、原兒茶醛等),這些成分之間存在潛在的協同作用,因丹參有美白淡斑及加速傷口癒合作用,因此也廣泛被用於美容產品上。本研究以中草藥丹參水溶液為介質,利用微分子美顏槍為導入工具,以達到皮膚淡斑及提升保水度之功效。

方法
收案20位年齡介於20~60歲男女性,隨機分成實驗組及對照組各10人,實驗組以2%丹參水溶液為介質,對照組則以生理食鹽水為介質,兩組均以微分子美顏槍為導入儀器。實驗進行前,會以CK皮膚檢測儀進行檢測原始皮膚「黑色素」及「保水度」指數狀況。
兩組基本資料如下:實驗組男:女=4:6,平均年齡47±7.77歲,對照組男:女=5:5,平均年齡46±9.79歲,P值0.813。平均黑色素數值;實驗
組17.4±3.1,對照組17.11±2.92,P值0.82。平均保水度數值;實驗組64.4±8.84,對照組62.82±3.85,P值0.64。
實驗週期共進行四週;每次施打後一週利用「CK皮膚檢測儀」進行效果評估,將測試四週的皮膚「黑色素」及「保水度」指數參數與第零週皮膚指數參數相比較,以獨立t檢定統計分析資料。
結果
實驗組經過四週測試後:發現第一週的平均黑色素指數與原始平均黑色素指數相比,並無統計學上差。但測試第二週、第三週後的平均黑色素指數與第零週平均黑色素指數相比,均明顯下降,P值<0.05。第四週的測試發現平均黑色素指數有上升趨勢,但與第零週平均黑色素相比,仍然有明顯下降P值0.0054。以保水度指數分析發現,不管對照組或實驗組,與第零週保水度參數相比較,都沒有明顯差異,P值大於0.05。
丹參結合霧化方式投與,將創造更精準、更快速、更有效性的途徑,將提供臨床另一種不一樣的選擇。
結論
2%丹參水溶液萃取經霧化方式導入,觀察黑色素指數從第二週開始確實有下降及改善情形,但是到了第四週,平均黑色素指數有上升趨勢,來到13.1,與第三週黑色素指數相比上升了2.7,但與初始(第零週)平均黑色素指數相比,下降了4.3以獨立t檢定相比,P值達0.0054,仍然有統計學上的差異。至於保水度方面,不管實驗組或對照組在經過四周測試後,與第零週平均值保水度相比,均無統計上的差異,P值均大於0.05。


background
Since ancient times, it is human nature to love beauty. With the changes of the times, modern people pursue beauty from the inside to the outside, and the internal requirements are to maintain health; various whitening and anti-aging health foods have been produced in response, and the external requirements are for immediate results; In order to meet the needs of all parties in medical cosmetology, a variety of rapid skin care lasers are available. Therefore, all whitening and antioxidant ingredients that can be applied externally or taken internally have become synonymous with youthfulness, and everyone is flocking to them.
Salvia miltiorrhiza is a Chinese herbal medicine with a long history and belongs to the Lamiaceae family. There are lipophilic components (tanshinone I, tanshinone IIa, tanshinone IIb, cryptotanshinone, dihydrotanshinone, etc.) and hydrophilic components (tanshinone, salvianolic acids A and B, protocatechuic aldehyde, etc.). There is a potential synergistic effect between Salvia miltiorrhiza and Salvia miltiorrhiza. Because Salvia miltiorrhiza can whiten spots and accelerate wound healing, it is more widely used in beauty products. This study uses Chinese herbal medicine Salvia miltiorrhiza aqueous solution as the medium and a micromolecule beauty gun as the introduction tool to achieve the effects of skin lightening and improving water retention.
method
Twenty men and women aged between 20 and 60 years old were admitted and randomly divided into an experimental group and a control group of 10 people each. The experimental group used 2% Salvia miltiorrhiza aqueous solution as the medium, while the control group used physiological saline as the medium. Both groups were treated with micron The molecular beauty gun is an imported instrument. Before the experiment, a CK skin tester will be used to detect the "melanin" and "water retention" index conditions of the original skin.
The basic information of the two groups is as follows: experimental group male:female=4:6, average age 47±7.77 years old, control group male:female=5:5, average age 46±9.79 years old, P value 0.813. The average melanin value was 17.4±3.1 in the experimental group and 17.11±2.92 in the control group, P value 0.82. The average water retention value was 64.4±8.84 in the experimental group and 62.82±3.85 in the control group, P value 0.64.
The experimental period lasted for four weeks in total; a CK skin tester was used to evaluate the effect one week after each application. The skin "melanin" and "moisture retention" index parameters for the four weeks of testing were compared with the original.
The skin index parameters were compared and the data were statistically analyzed using independent t test.
result
After four weeks of testing, the experimental group found that the average melanin index in the first week was not statistically different from the original average melanin index. However, the average melanin index after the second and third weeks of testing decreased significantly compared with the original average melanin index, with P value <0.05. The fourth week of testing found an upward trend in the average melanin index, but there was still a significant decrease compared to the original average melanin with a P value of 0.0054.
According to the analysis of water retention index, it was found that there was no significant difference between the control group and the experimental group compared with the original water retention parameters, and the P value was greater than 0.05.
conclusion
2% Salvia miltiorrhiza aqueous solution was introduced through atomization. It was observed that the melanin index did decrease and improve from the second week. However, by the fourth week, the average melanin index showed an upward trend and reached 13.1, which was similar to the melanin index in the third week. The ratio increased by 2.7, but compared with the initial average melanin index, it dropped by 4.3. Compared with the independent t test, the P value 0.0054, and there was still a statistical difference.
As for the water retention, there was no statistical difference between the experimental group and the control group after four weeks of testing compared with the average water retention at week zero, and the P> 0.05.

目 錄
誌 謝 i
摘 要 iii
Abstract vi
第一章 緒論 - 1 -
第一節 研究動機與背景 - 1 -
第二節 研究目的 - 3 -
第三節 研究流程 - 4 -
第二章 文獻探討 - 10 -
第一節 丹參的相關探討 - 11 -
第二節 皮膚結構與物質吸收屏障的探討 - 14 -
第三節 物質透過無針式經皮途徑的運用與探討 - 15 -
第四節 美白成分在皮膚的運用與影響 - 16 -
第五節 皮膚老化與黑色素的機轉探討 - 18 -
第六節 中草藥成分對抗光老化作用及機制 - 19 -
第七節 丹參與黑色素的相關機轉探討 - 20 -
第三章 研究方法 - 22 -
第一節 研究對象 - 22 -
第二節 研究架構 - 24 -
第三節 研究材料與工具 - 25 -
第四章 研究結果 - 29 -
第一節 資料分析 - 30 -
第二節 研究結論 - 36 -
第三節 討論與研究限制 - 38 -
參考文獻 - 41 -

圖目錄

圖一、研究流程 - 4 -
圖二、收案過程 - 6 -
圖三、實驗設計與操作流程 - 8 -
圖四、 微分子美顏 - 26 -
圖五、CK皮膚檢測儀 - 28 -
圖六、實驗組與對照組每週平均黑色素指數與初始(第零週)黑色素指數相比結果 - 31 -
圖七、實驗組及對照組保水度指數與第零週平均保水度指數相比 - 32 -
表六、TWO WAY ANOVA分析實驗組及對照組兩組受試者因不同介入因子影響後的結果 - 33 -
圖八、實驗組及對照組因介入因子及介入時間對黑色素指數的相關性 - 33 -
圖九、經過丹參霧化的四週測試後斑點淡化照片 - 34 -
圖十一、對照組與實驗組四次施打後平均黑色素指數相比 - 38 -

表目錄
表一、實驗組及對照組資料比較 - 29 -
表二、TWO WAY ANOVA分析性別、年齡與皮膚黑色素及保水度相關性 - 30 -
表三、實驗組與對照組每週平均黑色素指數與初始(第零週)黑色素指數相比結果 - 30 -
表四、實驗組與對照組每週平均保水度指數與初始(第零週)保平均保水度指數相比 - 31 -
表五、TWO WAY ANOVA分析實驗組及對照組時間和介入因子對兩組交互作用的影響 - 32 -




參考文獻
林宜信(2005)。中藥用藥安全與實務,行政院衛生署。
陳秀榮(2011)。化粧品添加中藥材研究,中醫年報,29(5),CCMP95-TP-045。
張聰民(2011)。中草藥可添加於化妝品之品項資料研究與安全性及有效性評估,中醫年報,9(5),CCMP95-TP-046。
鄧紅(2006)。本草綱目醫療美容方法簡論,時珍國藥研究,4:1。
孫海峰(2012)。丹參酚酸作為美白祛斑及抗皺化妝品添加劑的新用途專利申請
蘇宜婕.(2012)。本土丹參在化粧保養品之應用.全國碩博士資訊網
閻世翔(1995)。化妝品科學(上冊),北京科學技術文獻出版社pp.261~265;291。
行政院衛生署中醫藥委員會學術暨臨床應用研討會成果彙編(2004)。1~5冊。
Ansh,D.R., Sadhna,D., Nagpaal,L., & Chawla.(2015). Needle free injection technology: A complete insight. Pharmaceutical,5(4), 192-199.
http//doi/10.4103/2230-973X.167662.
Ansary,T.M., Hossain,M.R., Kamiya,K., Mayumi,K., & Ohtsuki,M.(2021). Inflammatory Molecules Associated with Ultraviolet Radiation-Mediated Skin Aging.Cosmetic,22(8),3974.https//doi/10.3390/ijms22083974.
Cristina,D., Aniello,F., Cermola,E.J., & atriarca,G.M.(2017).Vitamin C in Stem Cell Biology: Impact on Extracellular Matrix Homeostasis and Epigenetics.Stem Cells International,8936156.http//doi/10.1155/2017/8936156.
Chen,Y.S., Lee,S.M., Lin,Y.J., Chiang,S.H., & Lin,C.C.(2014).Effects of Danshensu and Salvianolic Acid B from Salvia miltiorrhiza Bunge (Lamiaceae) on Cell Proliferation and Collagen and Melanin Production.Molecules,89(9), 2029-2041.https//doi/10.3390/molecules19022029.
Ferreira,D., Videira,S., Filipe,D.L., & Moura,S.M.(2013).Mechanisms regulating melanogenesis. An Bras Dermatol,88(1),76-83. https://doi/10.1590/S0365-05962013000100009.
Fatih,I., Kurt,S., Serhat,I., & Merdan,S.(2018).Beneficial effects of Salvia miltiorrhiza in the healing of burn wounds: an experimental study in rats. Cosmetic,52(4),229-233.https://doi/10.1080/2000656X.2018.1461631.
Hu,S., Li,Z., Cores,J., Huang,J., Su,J., Phuong,U.D., & Cheng,K.(2019). Needle-Free Injection of Exosomes Derived from Human Dermal Fibroblast Spheroids Ameliorates Skin Photoaging. Access, 13(10),11273–11282. https://doi/10.1021/acsnano.9b04384.
Hani,Y., Alhajj,M., & Sharma,S.(2022).Anatomy Skin (Integument) Epidermis. Ethnopharmacology,30(10),987-1001.
Jing,Y., Du,Y., Meixu,W., Zhi,L., Yanxin,Z., Li,D., Pengwei,Z., & Aichun,J.U.(2021). Research progress on anti-inflammatory pharmacological action of components of Salvia miltiorrhiza and its preparations. American Chemical Society,44(11), 667-676.
Kesfeldt,J.L., Alexander,J., & Michels,B.F.(2014).The Roles of Vitamin C in Skin Health. American Society for Nutrition,16–18.
https://doi/10.3945/an.113.005157.
Kim,J.C., Jun,T.P., & Kang,H.Y.(2022).Skin-Aging Pigmentation:Who Is the Real Enemy Cell,11(16), 2541.
Li,Z.M., & Liu,P.Q.(2018).Salvia miltiorrhiza Burge (Danshen): a golden herbal medicine in cardiovascular therapeutics.Pharmacology,39(6),802–824.
https://doi/10.1021/acsnano.9b04384.
Logomasini,M.A., Richard,R.S., & Marcinkoski,R.(2013).Jet injection devices for the needle-free administration of compounds, vaccines, and other agents.JIPC,17(4).
Li,L., Luo,P., Zhao,W.J., Long,X.C., & Brinckmann,J.A.(2020).There ain't no mountain high enough?The drivers, diversity and sustainability of China's Rhodiolatrade. Ethnopharmacology,252(24). https://doi.org/10.1016/j.jep.2019.112379.
Lee,J.Y., Kim,E.D., Chan,H., Yo,T., Takashi,T., Dong,S., Yu,D.K., Mo,S., & Chung,H.(2015).Upregulation of Collagen Expression via PPARβ/δ Activation in Aged Skin by Magnesium Lithospermate B from Salvia miltiorrhiza. American Chemical Society and American Society of Pharmacognosy,78(8), 1112-1130.
https://doi:10.1021/acs.jnatprod.5b00348.
Masaki,S.(2010).Role of antioxidants in the skin: anti-aging effects. Journal of Dermatological Science,58(45),85–90.
Raisi1,A.O., Dezfoulian,F., Davoodi1,S., Taheri1,S., & Afshar,G.(2021). Salvia miltiorrhiza hydroalcoholic extract inhibits postoperative peritoneal adhesions in rats. BMC Complementary Medicine and Therapies,21(1),126-130.
http//doi/10.1186/s12906-021-03300-7.
Rorteau,J., Chevalier,F.P., Fromy,B., & Lamartine,J.(2020).Functional integrity of aging skin, from cutaneous biology to anti-aging
strategies.Med Sci,36(12),1155-1162.
https://doi/10.1051/medsci/2020223.
Sun,G.,Wang, J., Xu,X., Zhai,L., Li,Z., Liu,J., Zhao,D., Jiang,R., & Sun,J.(2023).Panax ginseng Meyer cv. Silvatica phenolic acids protect DNA from oxidative damage by activating Nrf2 to protect HFF-1 cells from UVA-induced photoaging. Ethnopharmacology,302(10),112-117. https://doi.org/10.1016/j.jep.2022.115883.
Stachowiak,J.C., Li,T.H., Arora,A., Mitragotri,S., & Fletcher,D.A.(2009). Dynamic control of needle-free jet injection.Journal of Controlled Release,135(2),104-112. https://doi.org/10.1016/j.jconrel.2009.01.003.
Smit,N., Vicanova,J., & Pavel,S.(2009).The hunt for natural skin whitening agents.Cosmetic,10(12), 5326-5349.https://doi.org/10.3390/ijms10125326.
Shukla,J., Upmanyua,N., Agrawalb,M., Swarnlata,S., Shailendra,S.D., & Alexanderb,A.(2018).Biomedical applications of microemulsion through dermal and transdermal route. Biomedicine & Pharmacotherapy,1477-1494. https://doi.org/10.1016/j.biopha.2018.10.021.
Sun,D., Wang,J., Xu,X., Zhai,X., Li,Z., Liu,J., Zhao,D., Jiang,R., & Sun,L.(2022).Panax ginseng Meyer cv. Silvatica phenolic acids protect DNA from oxidative damage by activating Nrf2 to protect HFF-1 cells from UVA-induced photoaging. Journal of Ethnopharmacology,87(6),276-280. https://doi.org/10.1016/j.jep.2022.115883.
Wu,Y.W., Ni,Z.U., Shi,Q.W., Dong,M., Kiyota,H., Gu,J., & Cong.B.(2012).Constituents from Salvia Species and Their Biological Activities.American Chemical Society,112(1),5967-2062.
Wang,Y.L., Hu,W., Zhang,Q., Yang,Y.X., Li,Q.Q., Hu,Y.J., Chen,H., & Yang,F.Q.(2018).Screening and Characterizing Tyrosinase Inhibitors from Salvia miltiorrhiza and Carthamus tinctorius by Spectrum-Effect Relationship Analysis and Molecular Docking. Chemistry.2141389.https//doi/10.1155/2018/2141389.
Wei,Z.X., Liu,Y.P., Hua,M.Z., Wang,X.N., Zhao,C.Q., Chen,J.M., & Liu,P.(2020).Pharmacological Effects of Salvianolic Acid B Against Oxidative Damage.Frontiers in Pharmacology,8(11),https//doi/10.3389/fphar.2020.57237.
Wang,D., Luc,X.E., Wang,B., Shi,L.B., Chi,M.B., & Tan,X.(2021).Salvianolic acid B attenuates oxidative stress-induced injuries in enterocytes by activating Akt/GSK3β signaling and preserving mitochondrial function.European Journal of Pharmacology,67(909),78-83.http//doi.org/10.1016/j.ejphar.2021.174408.
Xiao,D.M., Cao,Y.F., Che,Y.Y., Li,J., Shang,Z.P., Zhao,W.J., Qiao,Y.J., & Zhang,J.U.(2019).Danshen:a phytochemical and pharmacological overview.Chin J Nat Med,17(1), 59-80.

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊