臺灣博碩士論文加值系統

薑黃素這種天然生物活性化合物因其抗菌、抗氧化特性而聞名，透過奈米化改改善其生物相容性差以及水溶性弱等缺點能夠增強其生物應用潛力。自組裝過程可確保其形成穩定、且生物相容性高的奈米顆粒，為薑黃素奈米劑型的一個理想平臺。此外，奈米銅粒子具有強大的抗菌特性，因而備受關注。它們能夠產生活性氧（ROS）並破壞微生物的細胞膜。基於以上，我們期望能合成出新穎且穩定奈米粒子用於破壞生物膜的形成，抑制生物膜相關病原體的生長。在此研究，我們開發出集抗菌、抗氧化和抗發炎功能於一體的多功能奈米粒子。其通過溫和的碳化和溶劑注入將薑黃素（Cur）自組裝成薑黃素奈米粒子（Cur-NPs），並具有出色的抗氧化性能。此外，我們更進一步在薑黃素奈米粒子中嵌入氧化銅（I），形成了薑黃素複合氧化亞銅奈米粒子，進一步提高了它們的生物活性。由於增強了抗菌效果，Cur-NPs/Cu2O對各種細菌的最小抑菌濃度顯著提高，比薑黃素或Cur-NPs單獨使用時低約125倍。這些奈米粒子還能有效減少微生物群體感應中的色素產生，證實其能抑制細菌生物膜的形成。總之，Cur-NPs/Cu2O的合成有望推動生物膜抑制應用的發展，並為對抗生物膜相關感染的策略增添了有價值的內容。

Curcumin, a natural bioactive compound, is well known for its antibacterial and antioxidant properties. Improving its biocompatibility and water solubility through nano-formulation can enhance its potential for biological applications. The self-assembly process ensures the formation of stable, highly biocompatible nanoparticles, an ideal platform for curcumin nanodosage forms. In addition, copper nanoparticles are of interest because of their powerful antimicrobial properties. They are capable of generating reactive oxygen species (ROS) and disrupting the cell membranes of microorganisms. Based on the above, we expect to synthesize novel and stable nanoparticles for disrupting biofilm formation and inhibiting the growth of biofilm-associated pathogens. In this study, we report the development of multifunctional nanoparticles, integrating antimicrobial, antioxidant, and anti-inflammatory capabilities. The nanoparticles were synthesized by self-assembled curcumin (Cur) into curcumin nanoparticles (Cur-NPs) through a mild carbonization and solvent injection process, imparting excellent antioxidative properties. Building upon this platform, we further enhanced their efficacy by embedding copper (I) oxide (Cu2O) nanoclusters within Cur-NPs, resulting in Cur-NPs/Cu2O. The Cur-NPs/Cu2O exhibited a remarkable minimum inhibitory concentration against various bacteria, approximately 125-fold lower than curcumin or Cur-NPs alone, owing to their enhanced antimicrobial effect. These nanoparticles could also effectively suppress the production of pigment of quorum sensing to inhibit the formation of bacterial biofilm. In summary, the synthesis of Cur-NPs/Cu2O holds great promise for advancing biofilm inhibitory applications and adds valuable content to strategies to combat biofilm-related infections.

摘要 I
Abstract II
Table of Contents III
1 Introduction 1
1-1 Biofilm 1
1-1-1 The Formation of Biofilm 1
1-1-2 The Treatment and Prevention of Biofilm 3
1-2 Polyphenols 3
1-2-1 Curcumin 4
1-2-2 Bioactivities of Curcumin 5
1-3 Self-assembled Nanomaterials 6
1-3-1 Synthesis Mechanisms of Self-assembled Nanomaterials 6
1-3-2 Antimicrobiol Activities of Self-assembled Nanomaterials 7
1-4 Carbon Nanomaterials and their Antimicrobial Activities 8
1-5 Research Motivation 9
2 Materials and Methods 10
2-1 Chemicals and Materials 10
2-2 Instruments and Equipments 10
2-3 Preparation of Self-assembled Nanomaterials 11
2-4 Charaterization of Cur-NPs/Cu2O 11
2-5 Antibacterial Assay 12
2-6 Biofilm Inhibition Assay 12
2-7 DPPH Radical Scavenging Assay 12
2-8 Folin-Ciocâlteu Assay 12
2-9 Oxidase-like Activity Assay 13
2-10 Peroxidase-like Activity Assay 13
2-11 Bacterial Pigment Assay 13
2-12 Statistical Analysis 13
3 Results and Discussion 14
3-1 Characterization of Cur-NPs/Cu2O 14
3-2 Superior Antimicrobial Activity of Cur-NPs/Cu2O 16
4 Conclusions 19
Charts in this Article 20
References 34

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