臺灣博碩士論文加值系統

Contentiii
Figure captionsvi
Table captionsviii
Chapter 1. Literature review1
1.1 Electrospinning1
1.1.1 Introduction of electrospinning1
1.1.2 Factors affecting the electrospinning process3
1.1.3 Electrospinning in Wound Dressing and Hemostasis5
1.2 Electrospinning materials review7
1.2.1 Poly(vinyl alcohol) (PVA)7
1.2.2 Chitosan (CS)8
1.2.3 Gelatin (GEL)9
1.2.4 Glutaraldehyde (GA)10
1.2.5 Tranexamic acid (TXA)12
Chapter 2. Research motivation13
Chapter 3. Experimental section15
3.1 Materials and reagents15
3.2 Apparatus16
3.3 Preparation of nanofiber17
3.3.1 Preparation of PVA solution17
3.3.2 Preparation of CS/GEL solution17
3.3.3 Preparation of PVA-CS/GEL (PCG) solution17
3.3.4 Preparation of TXA-PVA-CS/GEL (T-PCG) solution17
3.3.5 Electrospinning18
3.4 Preparation of crosslinking nanofiber18
3.4.1 GA Vapor treatment18
3.4.2 GA Solution treatment18
3.5 Characterization of PVA/CS/Gel (PCG) nanofiber19
3.5.1 Nanofiber Morphology19
3.5.2 Fourier Transform Infrared Spectroscopy (FTIR)19
3.5.3 Microforce Testing System (MTS)19
3.5.4 Contact angle20
3.5.5 Swelling Ratio and Moisture Content20
3.6 Hemostasis of PCG-TXA nanofiber21
3.6.1 Whole Blood Clotting Coagulation21
3.6.2 Whole Blood Clotting Time21
3.6.3 Hemolysis assay in vitro22
3.6.4 Interfacial Interactions with Blood Cells22
3.7 Cytocompatibility of PCG Nanofiber23
Chapter 4. Result and discussions24
4.1 Morphology and fiber diameter24
4.1.1 PVA/CS/GEL (PCG) nanofiber24
4.1.2 TXA-PCG nanofiber27
4.1.3 Crosslinked PCG fiber28
4.2 Characterization of PVA/CS/Gel (PCG) nanofiber29
4.2.1 Fourier Transform Infrared Spectroscopy (FTIR)29
4.2.2 Microforce Testing System (MTS)31
4.2.3 Contact angle32
4.2.4 Swelling Ratio and Moisture Content34
4.3 Hemostasis of PCG-TXA nanofiber36
4.3.1 Whole Blood Clotting Coagulation36
4.3.2 Whole Blood Clotting Time37
4.3.3 Hemolysis assay in vitro38
4.3.4 Interfacial Interactions with Blood Cells39
4.4 Cytocompatibility of PCG Nanofiber41
Chapter 5. Conclusion42
References44

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