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研究生:于淑芬
研究生(外文):Yu, Shwu Feng
論文名稱:德基水庫多甲藻色素分析研究
論文名稱(外文):Pigment Analysis on Peridinium bipes in Techi Reservoir
指導教授:陳伯中陳伯中引用關係
指導教授(外文):Pei Chung Chen
學位類別:碩士
校院名稱:國立中興大學
系所名稱:植物學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:1998
畢業學年度:86
語文別:中文
論文頁數:60
中文關鍵詞:多甲藻色素
外文關鍵詞:pigmentPeridinium bipes
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台灣中部德基水庫水華發生之主要原因為多甲藻(Peridinium bipes
Stein fa. occlatum)大量聚集的結果,其細胞內含物中,全醣量為4.49ng
cell-1,全量蛋白質為4.98x10-3ng cell-1;細胞內氮:碳比值為0.01,比其
他種類甲藻低10倍左右,而在葉綠素a:碳則為0.5,比其他種甲藻多10倍;以
上測定結果,可能是多甲藻成為德基水庫之優勢種的原因之一;其他細胞內
含物含量如DNA為0.15ng cell-1,RNA為0.14ng cell-1,可溶性蛋白質
為0.63ng cell-1.利用薄層色層分析(TLC)的方式分離P.bipes 所含色素,
發現有12種色素,其中有8種為類胡蘿蔔素,且主要類胡蘿蔔素為
peridinin,而其葉綠素為葉綠素a及c2.將TLC上色素溶於不同有機溶劑,如
酒精 丙酮 乙醚 正己烷 苯 石油醚及甲醇等,以測定其最大吸光值,並配
合質譜儀的分析技術,以判定P.bipes. 所含色素種類,發現其類胡蘿蔔素
含有甲藻門常見的B-carotene diadinoxanthin diatoxanthin 及
neochrome, 還有再甲藻門比較少見的mutatoxanthin及
chrysanthemaxanthin,更含有少量(1.25-1.27% Total carotenoids)在
Prasinophyceae 中主要的類胡籮蔔素-prasinoxanthin;另有一種顏色為
淡黃色之色素則未知.利用高效能液相色層分析(HPLC)測知各色素含量,發
現P.bipes.之peridinin含量在細胞內佔總類胡蘿蔔素之比例較其他種甲
藻少,且在其他種甲藻含量豐富之diadinoxanthin(3.8-33.2%),在P.
bipes.含量亦很少,大約只有1.3-2.5%. P.bipes.所含之類胡蘿蔔素以
mutatoxanthin(21.7-30.8%) peridinin(30.0-44.0%) B-
carotene(6.8-15.3%)及diatoxanthin(5.8-17.9%)含量最多,幾乎佔所有
類胡蘿蔔素90%以上.在HPLC色素測定中,發現1997年7月及10月所採集之多
甲藻,色素總量不同,且在各色素相對量之分配上也不相同,此現象究竟與
細胞生長週期 季節性之改變,或採樣不均等原因有關,則有待後人加以研
究探討.
A dinoflagellate, Peridinium bipes Stain fa. occulatum usually
forms bloom in Techi Reservoir in summer. It is interesting to
know the photosynthetic pigmentof this species. After
calculation the ratio of nitrogen to carbon of P.bipes cellswas
about 10 fold lower than that of reported dinoflagellates. The
ratio of chlorophyll a to carbon was 10 fold higher than other
dinoflagellates. These date might indicate that the
photosynthetic products of P.bipes was transferred to the need
of cell growthTwelve pigments of P.bipes were separated from
thin-layer chromatography. Each pigment was identified by
visiblelight spectrometry and mass spectrometry(inclucding EI MS
and FAB MS).It was found that P.bipes contained 8 carotenoids,
including diadinoxanthin,mutatoxanthin,diatoxanthin,B-carotene,
prasinoxanthin,chrysanthemaxanthin,neochrome and peridinin,in a
addition to two major chlorophylls,chlorophull a and chlorophyll
c2.Quantitative analysis of the carotenoids profile in P.bipes
was also studies by a reversed-phase HPLC.Four major carotenoids
were found to be peridinin(30.0-44.0%),
mutatoxanthin(21.7-30.8%),B-carotene(6.8-15.3%) and
diatoxanthin(5.8-17.9%). However accordingly the content of
total amounts of these four carotenoids were found to exceed 90%
in carotenoids of P.bipes.Peridinin and diadinoxanthin in P.
bipes were found to be relatively small in comparison with those
of other reported dinoflagellates.
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