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研究生:徐國凱
研究生(外文):Kuo-Kai Hsu
論文名稱:應用葉綠體DNA變異探討台灣原生杜鵑之親源關係
論文名稱(外文):Phylogenetic Relationships of Taiwan Native Rhododendron spp. (Ericaceae) Based on Chloroplast DNA Variation.
指導教授:黃士穎
指導教授(外文):Shih-Ying Hwang
學位類別:碩士
校院名稱:中國文化大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:55
中文關鍵詞:葉綠體DNA分子親源關係杜鵑
外文關鍵詞:chloroplast DNAmolecular phylogeneticsRhododendron
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本研究利用cp DNA PCR/RFLP與AFLP分子標記及cpDNA之trnF-trnL基因間區間序列片段分析八種台灣原生杜鵑之親源關係。
在cp DNA PCR/RFLP分子標記部分,以限制Hinf I、Sau3A I、Hae III、Hpa II、Dde I、Rsa I作用葉綠體DNA之trnS4-trnT片段後,所得譜相同,說明杜鵑花之葉綠體DNA在此片段之高度相似性。以玉山杜鵑、森氏杜鵑及紅星杜鵑為材料進行cpDNA之AFLP分子標記分析,結果顯示三者之遺傳距離十分相近。
八種杜鵑trnF-trnL基因間區間序列排序後之全長為460個氮鹼基對,此區域內有41處變異,其中10處可以做為親源關係判別用。核酸序列排序後以MEGA軟體進行UPGMA及Neighbor-joining樹狀親源關係圖的分析。由UPGMA及Neighbor-joining親源樹狀圖均可得到兩個主要的分群。第一群包含玉山杜鵑、森氏杜鵑、紅星杜鵑、南湖杜鵑及台灣杜鵑,其中台灣杜鵑可自此群單獨分出為一群;第二群包括西施花、金毛杜鵑及烏來杜鵑,其中金毛杜鵑與烏來杜鵑的親源關係較為接近。
本研究之主要結果為藉由分子證據顯示玉山杜鵑、森氏杜鵑及紅星杜鵑之間有非常接近的親源關係,而此結果亦獲得形態學上的支持。
PCR/RFLP, AFLP and sequence comparisons of the chloroplast trnF-trnL intergenic spacer region were used to analyze phylogenetic relationships among specimens of the 8 native species of Rhododendron in Taiwan.
Polymerase chain reaction (PCR) amplified chloroplast intergenic spacer trnS4/trnT region was treated with 6 restriction enzyme including Hinf I、Sau3A I、Hae III、Hpa II、Dde I and Rsa I. Restriction fragment of trnS4/trnT revealed no difference between R. pseudochrysanthum, R. morii and R. rubropunctatum. AFLP marker revealed highly similarity also.
Aligned sequences of the 8 Rhododendron taxa resulted in 460 characters. The region contained 41 variable sites and of which 10 were phylogenetically informative. UPGMA and Neighbor-Joining trees were constructed with MEGA. Two major clusters obtained from UPGMA and Neighbor-Joining trees: cluster 1 consists of R. Pseduchrysanthum, R. morii, R. rubropunctatum, R. hyperythrum, and R. formosanum, R. formosanumcan be further distinguished from the other 4 Rhododendron in cluster 1. Cluster 2 consists of R. ellipticum, R. oldhamii, R. kanehirai and with the later two had closer phylogenetic relationship.
The major result of this study was that R. pseudochrysanthum, R. morii, and R. rubropunctatum had very close phylogenetic relationship and this result supported the observation of morphological characters.
圖目錄 I
表目錄 II
摘要 IV
Abstract V
壹、前言 1
貳、前人研究 3
一、台灣地區原生杜鵑之發現與相關研究 3
二、台灣地區具爭議的杜鵑分類問題 6
三、分子標記的應用 8
四、葉綠體DNA於親源關係上的應用 10
參、材料與方法 13
一、實驗材料的取得與處理 13
二、DNA的萃取 13
三、DNA的定量與稀釋 15
四、cp DNA PCR/RFLP 15
五、cp DNA AFLP 21
六、cp DNA PCR產物定序 25
七、分析方法 25
肆、結果 28
一、cp DNA PCR/RFLP部份 28
二、cp DNA AFLP的結果 29
三、cp DNA片段定序結果 36
伍、討論 44
一、紅星杜鵑、玉山杜鵑與森氏杜鵑之相似性 44
二、杜鵑花在葉綠體DNA trnF-trnL片段核酸定序上的變異 44
三、分子分類與型態分類之比較 47
四、地理分佈之探討 49
陸、參考文獻 50
呂勝由、楊遠波(1989) 台灣杜鵑花屬植物之訂正 林業試驗所研究報告季刊4 (4) : 155-166
林素夷(1991)唐杜鵑、大屯杜鵑與中原杜鵑的電泳與葉部型態分析 國立台灣大學森林學研究所資源保育組碩士論文
陳玉峰(1995)台灣植被誌 第一卷:總論及植被帶概論 pp 20-25
馮國楣(1988)中國杜鵑花 科學出版社 北京
黃麗虹(1999)紅檜與台灣扁柏葉綠體DNA變異 中國文化大學生物科技研究所碩士論文
劉業經、呂福原、歐辰雄(1994)台灣樹木誌 p.450-456 國立中興大學農學院出版委員會
劉棠瑞,廖日京(1981) 樹木學(下),p737- 台灣商業印書館
潘富俊(1988)玉山杜鵑和森氏杜鵑之海拔變異,中華林學季刊 21(2) : 99-102
蘇鴻傑(1984)台灣之植群:山地植群帶與地理氣候區 「台灣生物資源調查及資源管理研習會論文集」 中央研究院植物研究所專刊第十一號 pp 39-53
Bain, J.F. and R.K.Jansen, (1995) A phylogenetic analysis of the aureoid Senecio (Asteraceae) complex base on ITS sequence data. Plant Syst. Evol. 195: 209-219
Bower, C. G. (1960) Rhododendrons and Azaleas, 2nd ed. Macmillan, New York.
Clegg M. T., and G. Zurawski (1992) Chloroplast DNA and the study of plant phylogeny: present status and future prospects. In “ Molecular Systematics of Plants ” (P. S. Soltis, D. E. Soltis, and J. J. Doyle, Eds.), pp.1-13, Chapman & Hall, New York.
Clegg, M. T., B. S. Gaut, G. H. Learn, JR., and B. R. Morton (1994) Rates and patterns of chloroplast DNA evolution. Proceedings of the National Academy of Sciences, USA 91: 6795-6801
Cros, J., M. C. Combes, P. Trouslot, F. Anthony, S. Hamon, A. Charrier and P. Lashermes (1998) Phylogenetic analysis of chloroplast DNA variation in Coffea L. Molecular Phylogenetics and Evolution 9 (1): 109-117
Curtis, S. E. and M. T. Clegg (1984) Molecular evolution of chloroplast DNA sequences. Molecular Biology and Evolution 1: 291-301
Dapeng Z., M. Ghislain, Z. Huaman, A. Golmirzaie, and R. Hijmans (1998) RAPD variation in sweetpotato (Ipomoea batatas (L.) Lam) cultivars from South America and Papua New Guinea. Genetic Resources and Crop Evolution 45: 271-277
De Riek J., J. Dendauw, M. Mertens, M. De Loose, J. Heursel and E. Van Blockstaele (1999) Validation of criteria for the selection of AFLP markers to assess the genetic variation of a breeders’ collection of evergreen azaleas. Theor Appl Genet 99: 1155-1165
Demesure B., N. Sodzi, R. J. Petit (1995) A set universal primer for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Molecular Ecology 4: 129-131
Doyle J. J. and J. L. Doyle (1990) Isolation plant DNA from fresh tissue. Focus 12: 13-15
Fehr, W. R. (1991) Principles of cultivar development, Vol. 1, Theory and Technique. Macmillan, New York.
Galle, F. C. (1987) Azaleas. Timber Press, Portland, Oregon.
Gauthier P., R. Lumaret and A. Bedecarrats (1998) Genetic variation and gene flow in Alpine diploid and tetraploid populations of Lotus (L. alpinus (D.C.) Schleicher/L.corniculatus L.) I. insights from morphological and allozyme marker. Heredity 80: 683-693
Hagemann R. and M. B. Schroder (1989) The cytological basis of the plastid inheritance in angiosperms. Protoplasma 152: 57-64
Hastenrath, S. (1968) Ceratin aspects of the three-dimensional distribution of climate and vegetation belts in the mountains of Central America and southern Mexico. Colloquium Geogr. 9: 122-130
Hayata, B. (1911-1921) Incones Plantarum Fomosanarum. Vols. I-X.
Henry, A. (1896) A list of plants from Formosa with some preliminary remarks on the geography, natural of flora and economic botany of the island. Trans. Asiat. Soc. Jap. 24 Suppl.: 1-118
Hirastsuka J., H. Shimada, R. Whittier, T. Ishibasshi, M. Sakamoto, M. Mori, C.Kondo, Y. Honji, C. R. Sun, B. Y. Meng, Y. Q. Li, A. Kanno, Y. Nishizawa, A. Hirai, K. Shinozaki and M. Sugiura (1989) The complete sequence of the rice (Oryza sativa) chloroplast genome: intermolecular recombination between distinct tRNA genes accunts for a major plastid DNA inversion during the evolution of cereals. Mol. Gen. Genet. 217: 185-194
Hsu, C. C. (1973) Biosystematic investigation on the Rhododendron of Taiwan . Proceedings of the National Science Council 6: 13-50
Iqbal J. M., D. W. Paden and A. L. Rayburn (1995) Clonal stability of RAPD markers in three Rhododendron species. J. Environ. Hort. 13: 43-46
Korn K. A., (1996) Phylogenetic relationships of Empetraceae, Epacridaceae, Ericaceae, Monotropaceae, and Pyrolaceae: evidence from nuclear ribosomal 18s sequence data. Ann. Bot. 77: 293-303
Li, H. L. (1963) Woody flora of Taiwan. Livingston Publ. Comp.
Li, H. L. (1978) Ericaceae. In Li. H. L., T. S. Liu, T. C. Huang, T. Koyama, and C. E. DeVo1 (eds) Flora of Taiwan. Vol 4: pp.21-38
Luschnig C. and D. Schweizer (1992) Nucleotide sequence of trnI(CAU) and rpl23 from Arabidopsis thaliana chloroplast genome. Nucleic Acids Res. 20: 3511
Lyamichev, V., Brow MAD, Dahlberg JE (1993) Structure-specific endonucleolytic cleavage of nucleic acids by eubacterial DNA polymerases. Science 260: 778-783
McDonald, S., (1992) Native azaleas of Georgia. J. Am. Rhod. Soc. 46: 146-149
Nei M. (1987) Molecular evolutionary genetics. Columbia Univ. Press, New York
Ohwi, J. (1937) Plantae novae Japonicae IV. Journ. Jap. Bot. 13: 332-343
Orita, M., Y. Suzuki, T. Sekiya and K. Hayashi (1989) Rapid and sensitive detection of point mutations and DNA polymorphism using the polymerase chain reaction. Genomics 5: 874-879
Palmer, J. D. and D. B. Stein (1986) Conservation of chloroplast genome structure among vascular plants. Current Genetics 10: 823-833
Palmer, J. D. (1991) Plastid chromosomes : structure and evolution. In L. Bogorad and I. K. Vasil [eds], vol. 7A, The molecular biology of plastid, 5-53, Cell culture and somatic cell genetic of plants. Academic Press, San Diego, California, USA.
Parducci, L., A. E. Szmidt (1999) PCR / RFLP analysis of cpDNA in the genus Abies Thero. Appl. Genet. 98: 802-808
Perera L., J. R. Russell, J. Provan, J. W. MeNicol, and W. Powell (1998) Evaluating genetic relationships between indigenous coconut (Cocos nucifera L.) accessions from Sri Lanka by means of AFLP profiling. Theor Appl Genet 96: 545-550
Plomion, C. P., P. Hurme, J-M. Frigerio, M. Ridolfi, D. Pot, C. Pionneau, C. Avila, F. Gallardo, H. David, G. Neutelings, M. Campbell, F. M. Canovas, O. Savolainen, C. Bodenes and A. Kremer (1999) Developing SSCP markers in two Pinus species. Molecular Breeding 5: 21-31
Petes, T. K. and D. Botstein. (1977) Simple mendelian inheritance of the reiterated ribosomal DNA of yeast. Prol. Natl. Acad. Sci., USA. 74: 5091-5095
Rayburn L. A., M. J. Iqbal, D. W. Paden, (1993) Positive identification of Rhododendron through DNA fingerprinting. J. Am. Rhod. Soc. 47: 137-138
Reboud, X., and C. Zeyl, (1994) Organelle inheritance in plants. Heredity 72: 132-140
Richard, I. M. and R. J. Abbott (2000) Origin and evolution of invasive naturalized material of Rhododendron ponticum L. in the British Isles. Molecular Ecology 9: 541-556
Sasaki, S. (1928) List of plants of Formosa. The Natural History Society of Formosa, Taihoku.
Scheiber S. M., R. L. Jarret, C. D. Robacker, M. Newman (2000) Genetic relationships within Rhododendron L. section Pentanthera G. Don based on sequences of the internal transcribed spacer (ITS) region. Scientia Horticulturae 85: 123-135
Shaw, C. R. and R. Prasad (1970) Starch gel electrophoresis of enzyme — a compilation of recipes. Biochem Genet 4: 297-320
Shimada H. and M. Sugiura (1991) Fine structural features of the chloroplast genome: comparison of the sequenced chloroplast genomes. Nucleic Acids Res. 19: 983-995
Shinozaki K., M. Ohme, M. Tanaka, T. Wakasugi, N. Hayashida, T. Matsubayashi, N. Zaita, J. Chunwongse, J. Obokata, K. Yamaguchi-Shinozaki, C. Ohto, K. Torazawa, B. Y. Meng, M. Sugita, H. Deno, T. Kamogashira, K. Yamada, J. Kusuda, F. Takaiwa, A. Kato, N. Tohdoh, H. Shimada, and M. Sugiura (1986) The complete nucleotide sequence of the tobacco chloroplast genome: Its gene organization and expression. EMBO J.5: 2043-2049
Stephen R. D., D. S. Katz-Downie and M. F. Watson (2000) A phylogeny of the flowering plant family Apiaceae based on chloroplast DNA RPL16 and RPOC1 intron sequences: towards a suprageneric classification of subfamily Apioideae American Journal of Botany 87(2): 273-292
Suzuki, Tokyo. (1935) Two new species of plants from Taiwan. Trans. Nat. Hist. Soc. Form. 25: 40-41
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalities and weight matrix choice. Nucl. Acids. Res. 22: 4673-4680
Vos P., R. Hogers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper and M. Zabeau (1995) AFLP: a new technique for DNA fingerprinting. Nucl. Acids. Res. 23(21) : 4407-4414
Wakasugi T., S. I. Tsudzuki, M. Shibata and M. Sugiura (1994) A physical map and clone bank of the black pine (Pinus thunbergii) chloroplast genome. Plant Molecular Biology Reporter 12: 227-241
Wang Xiao-Ru., Y. Tsumura., H. Yoshimaru., K. Nagasaka., and A. E. Szmidt. (1999) Phylogenetic relationships of Eurasian pines (Pinus, Pinaceae) based on chloroplast RBCL, MATK, RPL20-RPS18 spacer, and TRNV intron sequences. American Journal of Botany 86(12): 1742-1753
Whitmore, T. C. (1975) Tropical rain forests of far east. Oxford Univ. Press, London.
Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalsk, and S. V. Tingey. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids. Res. 18 (22): 6531-6535
Wilsion, E. H. (1925) The rhododendrons of Eastern China, the Bonin and Liukiu Islands and of Formosa. J. Arnold Arboretum 6: 156-185
Yamazaki, T. (1981) Some new taxa of Rhododendron from Japan and Taiwan. Journ. Jap. Bot. 56(11): 357-366
Ying S. S., (1976) The Ericales of Taiwan. Quart. Journ. Chin. For. 9(4): 107-137
Yonemori K., D. E. Parfitt, S. Kanzaki , A. Sugiura, N. Utsunomiya, and S. Subhadrabandhu (1996) RFLP analysis of an amplified region of cpDNA for phylogeny of the genus Diospyros J. Jap.Soc. Hortic Sci. 64: 771-777
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