跳到主要內容

臺灣博碩士論文加值系統

(44.221.66.130) 您好!臺灣時間:2024/06/24 06:26
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:陳幸宜
研究生(外文):Hsin-Yi Chen
論文名稱:Heatshockprotein-27在大老鼠視神經受損後在視網膜的表現
論文名稱(外文):Expression of heat shock protein -27 in rat retina after optic nerve axotomy
指導教授:王惠珠王惠珠引用關係
指導教授(外文):Hwei-Zu Wang
學位類別:碩士
校院名稱:高雄醫學大學
系所名稱:醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2001
畢業學年度:89
語文別:中文
論文頁數:47
中文關鍵詞:熱休克蛋白-27視神經受損
外文關鍵詞:heat shock protein -27optic nerve axotomy
相關次數:
  • 被引用被引用:0
  • 點閱點閱:185
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
熱休克反應( heat shock response) 一向被認為是在細胞體內甚至於是生物體內極其重要的保護機制。熱休克蛋白質( heat shock protein )它最早從果蠅基因中被分離出來, 之後許多研究都一直在探討heat shock protein 在生物體及細胞內的意義,再再都顯示heat shock protein 的引發與許多壓力狀態有關。目前發現至少有兩百種的誘導因子可促成熱休克蛋白質的合成,包括高溫, 缺氧,重金屬,自由基,病毒感染,發炎,氧化劑傷害,微生物感染,缺血,酸鹼中毒, 到這幾年大老鼠腦中風模式的研究,周邊神經系統受損的研究,青光眼病人的研究。這些都說明了heat shock protein 的產生可能是生物體內的一個重要的保護機制。
基於這樣的想法,在這篇研究中我們是應用免疫組織染色的方法去探討heat shock protein 27 在大老鼠視神經受損模式中的表現,希望能了解生物體內視神經受損的疾病模式中heat shock protein 27的角色,更期望從這篇研究中能對我們在臨床上面對的創傷性視神經病變的病人有另外的治療方向。
經過我們的研究發現,在視神經受損時,大老鼠體內的heat shock protein 27 的合成有增加 ,這讓我們了解到heat shock protein 27 在視神經受損可能有重要意義。此外我們也發現這些呈現heat shock protein 27(+)的免疫組織染色的細胞都集中在ganglion cell layer 及inner nuclear layer。這個結果說明了 heat shock protein 27在大老鼠視神經創傷可能有重要的保護機制,至於它真正的機轉在那,有待我們將來進一步的探討。此外, 這個發現也希望能對於我們將來視神經的再生研究有另一個重要的探討方向。

Heat shock response is traditionally thought of as an important protection mechanism in cell bodies and organisms. Although heat shock proteins ( HSPs) were initially isolated in Drosophila , it soon became evident that a heat shock response also occurred in mammalian cells. The inductions of heat shock proteins in previous series of studies all reveal the association of heat shock proteins with many kinds of stresses . So far, over two hundreds of induction factors were widely discussed , including hyperthermia , anoxia, heavy metals, free radicals, virus infection, inflammation, oxidative injury, microbial infection, ischemia, acid injury and alkaline injury. In recent years , more research focus on the association of heat shock protein with nervous system such as adult rat cerebral ischemia model, peripheral nerve injury model, and glaucoma .
Therefore, here we report a immunohistochemical method to discuss the heat shock protein 27 in optic nerve axotomy of adult rat. We expect that this model will provide us a new idea about the role of heat shock protein in optic nerve injury so as to understand the clinical application of heat shock protein in patients of traumatic optic neuropathy。
After our study, we found that increased immunostaining of heat shock protein 27 in optic nerve axotomy eyes may reflect a role of these proteins as a cellular defense mechanism in response to stress or injury in optic nerve axotomy. The results also inspire us a new direction to approach the optic nerve regeneration in the future。

第一章 前言 : 7
第二章 : 前人研究 12
第三章 : 研究材料與方法 15
第四章: 研究結果 19
第五章: 討論 22
第六章 : 結論 29
第七章: 參考文獻 30
表圖目錄: 37

1. Ritossa, F. M A new puffing pattern induced by a temperature shock and DNP in Drosophila. Experientia Basel 18 : 571-573 , 1962
2. Tiessieres, A., H.K . Mitchell , and U. Tracy . Protein synthesis in salivary glands of Drosopohila melanogaster: Relation to chromosome puffs . J. Mol. Bio. 84 : 389-398 , 1974
3. Craig E.A ., Jacobsen , K. Mutations of the heat-inducible 70 kilodalton genes of yeast confer temperature sensitive growth . Cell 38 : 841-49 , 1984
4. Linquist , S., Didomenico. B , Bugarslqy G , Kartx S, Petko L. Regulation of the heat shock response in Drosophila and yeast . In : Heat shock , From bacteria to Man, edited by M. J Shlesinger, M. Ashburner and A Tissieres. Gold Spring Harbor, NY L Cold Spring Harbor, P. 167-175 , 1982
5. Linquist S, Craig EA :The heat shock proteins Ann Rev. Genet 22: 631-77 , 1988
6. Subjeck JR, Shyy TT. Stress protein systems of mammalian cells. Am J Physiol. 250 : C1-C 17 , 1986
7. Welch WJ. Mammalian stress response : cell physiology structure/ function of stress proteins and implications for medicine and disease. Physiol Rev 72 : 1063-1081 , 1992
8. Anathan , J golderg L, Voelllmy R Abnormal proteins serve as eukaryotic stress signals and trigger the activation of heat shock protein genes. Science Wash DC 8: 223-230, 1973
9. Beckmann, R.P., Mizzen LA, Welch WJ. Interaction of hsp 70 with newly synthesized proteins : implications for protein folding and assembly events. Science wash DC 248 : 850-854 , 1990
10. Bardwell JC, Craig EZ Major heat shock protein gene of Drosophila and the heat inducible dnaK gene are homologous Proc Nato. Aca Sci USA 81: 848-849 , 1984
11. Smith , B.J and Yaffe MP Uncoupling thermotolerance from the induction of heat shock proteins. Proc Natl.Acad. Sci USA. 88: 11091-11094, 1991
12. Tezel G, Rosario HM , Martin B.W : Immunostaining of heat shock proteins in the retina and optic nerve head of normal and glaucomatous eyes Arch Ophthalmol 118 : 511-518, 2000
13. Tytell M, Barbe MF, Brwon IR: Induction of a heat shock( stress ) protein 70 and its mRNA in the normal and light damaged rat retina after whole body hyperthermia. N Neurosci Res. 38:19-31 , 1994
14. Hopkins DA, Plumier JCL , Currie RW : Induction of the 27-kDa heat shock protein ( HSP 27 ) in the rat medulla oblongata after vagus nerve injury Experimental Neurology 153 : 173-183, 1998
15. Kato H, Araki T, Itoyama Y, Kogure K, Kato K: An imunohistochemical study of heat shock proteins —27 in the hippocampus in a gerbil model of cerebral ischemia and ischemic tolerance . Neuroscience. 68: 65-71, 1995
16. Huot J , Roy G, Lambert H, Chretien P, Landry J Increased survival after treatments with anticancer agents of chinese hamster cells expressing the human Mr 27000 heat shock protein. Cancer Res 51: 5245-5252, 1991
17. Lavoie Jn, Gingras-Breton G, Tanguay RM, Landry J: Induction of chinese hamster HSP27 gene expression in mouse cells confers resistance to heat shock . HSP 27 stabilization of the microfilament organization J Biol Chem 268: 3420-3429 , 1993a
18. Mairesse N, Horman S, Mosselmans R, Galand P Antisense inhibition of the 27kDa heat shock protein production affects growth rate and cytoskeletal organization in MCF-7 cells. Cell Biiol Int 20: 205-212, 1996
19. De Jone WW, Leunissen JA, Leene PJ, Zweers A, Verseeg M Dogfish α—crystallin J Biol Chem 263: 5141-5149, 1988
20. Jacob U, Gaeslel M, Engel K, Buchner J: Small heat shock proteins are molecular chaperons. J Biol Chem 268: 1517-1520, 1993
21. Lavoie JN , Lambert H, Hickey E, Weber LA, Landry J : Modulation of cellular thermoresistance and actin filament stability accompanies phosphorylation-induced changes in the oligomeric structure of heat shock protein 27 . Mol Cell Biol 15: 505-516, 1995
22. Mehlen Pk Kretx-Remy C, Preville X, Arrigo A-P: Human hsp 27 , Drosophkila hsp 27 and human αβ-crystallin expression —mediated increase in glutathaione is essential for the protective activity of these proteins against TNF-α induced cell death . EMBO J 15: 2695-2706, 1996a
23. Samali A, Cotter TG Heat shock protein increase resistance to apoptosis . Exp Cell Res 223: 163-170, 1996
24. Mehle P, Chulze-Ostoff K, Arrigo A-P : Small stress proteins as novel regulator of apoptosis . J Biol Chem 271: 16510-16514, 1996b
25. Plumier JC, Armstrong JN , Landry J, Babity JM, Robertson HA, Currie RW: Expression of the 27, 000 mol. Wt heat shock protein following kainic acid-induced status epilepticus in the rat . Neuroscience 75: 849-856 , 1997
26. Higashi T, Takechi U, Uemura Y, Kikuchi H, Nagata K Differential induction of mRNA species encoding several classes of stress proteins following focal cerebral ischemia. Brain Res 650: 239-248, 1997
27. Clark BD, Brown IR. Axonal transport of a heat shock protein in the rabbit visual system. Proc Natl Acad Sci USA. 82 : 1281-1285, 1995
28. Benndorf R, Hayes K, Ryazantev S, Wieske M, Behlke J, Lutsh G: Phosphorylation and supramolecular organization of murine small heat shock protein hsp 25 abolish its actin polymerization —inhibiting activity. J Biol Chem 269: 20780-20784, 1994
29. Lavoie JN Hickey E , Weber LA, Landry J: Modulation of actin microfilament dynamics and fluid phase pinocytosis by phosphorylation of heat shock protein . J Biol Chem 268: 24210-24214, 1993b
30. Melen P, Chareyron P, Briolay J , Klemenz R, Arrigo AP Constitutive expression of human HSP27 , Drosophila HSP 27 , or human alpha B-crystallin confers resistance to TNF-α and oxidative stress-induced cytotoxicity in stably transfected murine L929 fibroblasts. J Immunol 154: 363-374 , 1995
31. Landry , J., and J Huot Modulation of actin dynamics during stress and physiological stimulation by signaling pathway involving p38 MAP kinase and heat-shock protein 27 . Biochem. Cell Biol. 73 : 703-707 , 1995
32. Renhawek K, Bosman G. J. C. G. M and W. W de Jon Expression of small heat shock protein hsp27 in reactive gliosis in Alzheimer disease and other type dementia . Acta Neuropathol 87 :; 511-519, 1994
33. Tezel G, Seigel GM, Wax MB Autoantibodies to small heat shock proteins in glaucoma Invest Ophthlamol Vis Sci 39; 2277-2287, 1998
34. Arrigo, AP and P Mehlen Expression , cellular location and function of low molecular weight heat shock proteins ( Hsp 20s) during development of the nervous system . In R. J. Mayer and I. R. Brown ( eds) : Heat shock proteins in the nervous system. San Diego : Academic Press, pp. 145-167 , 1994
35. Chretein , P., Landry J. Enhanced constitutive expression of the 27 dKD heat shock proteins in heat-resistant variants from Chinese hamster cells. J. Cell Physiol. 137 : 157-166, 1988
36. Plumier, JCL , Armstrong JN, Wood NI, Babity JM , Hamilton TC, Hunter AJ, Robertson HA, Currie RW Differential expression of c-fos , Hsp 70 , Hsp 27 after photothrombotic injury in the rat brain. Mol Brain Res 45 : 239-246, 1997
37. Klemenz R, Andres AC, Frohli E, Shafer R, Ayoama A Expression of the murine small heat shock proteins 25 and B-crystallin in the absence of stress J. Cell Biol 120 : 639-645, 1993
38. Costigan M, Mannion RJ, Kendal G, et al: Heat shock protein 27:developmental regulation and expression after peripheral nerve injury. J Neurosci. 18: 5891-5900 , 1998
39. Cauley KA, Sherman TG, Ford-Holevinski T, Agranoff BW. Rapid expression of novel proteins in goldfish retina following optic nerve crush. Biochem Biophysio Res Commun. 138 : 1177-1183, 1986
40. Kojuma M, Hoshimura M, Aoki T, et al: Expression of heat shock proteins in the developing rat retina. Neurosci Lett. 205:215-217, 1996

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top