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研究生:王壯維
研究生(外文):Chuang-Wei Wang
論文名稱:BRAK/CXCL14和RhoA在人類腎細胞癌之表現量探討
論文名稱(外文):The Expression of BRAK/CXCL14 and RhoA in Human Renal Cell Carcinoma
指導教授:侯自銓
指導教授(外文):Tzyh-Chyuan Hour
學位類別:碩士
校院名稱:高雄醫學大學
系所名稱:生物化學研究所碩士班
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2005
畢業學年度:93
語文別:中文
論文頁數:78
中文關鍵詞:腎細胞癌腫瘤標記
外文關鍵詞:RhoARCCtumor markerBRAK/CXCL14
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腎細胞癌(renal cell carcinoma,R.C.C)是一種不易治療的疾病,因具高度抗藥性,手術根治法雖可提高治療效果,癒後卻可能高達50%的復發率。如何利用偵測專一性的腫瘤標記分子之改變,早期診斷出腎細胞癌的病變,以期提高治療成效,是目前治療腎細胞癌重要課題。因此,我們用微陣列分析(cDNA microarray)的技術,於基因層級上,挑選BRAK/CXCL14和RhoA這二基因在正常細胞和癌細胞有差異表現,然後使用RT-PCR,real-time-PCR,Western blotting和immunohistochemistry…等技術,確認這二基因的表現。結果發現BRAK/CXCL14在腎細胞癌病人中,癌化部位和正常部位相比,基因和蛋白層次分別有72.22%和63.89%表現下降(down- regulated);細胞株方面,腎細胞癌細胞株(ACHN、A498、769-P、786-O)相對於趨近正常的近端腎小管細胞株(HK-2)有較低的表現。至於RhoA病人癌化部位和正常部位相比,基因和蛋白上則分別為58.33%和50.00%的病人表現上升(up-regulated)。三株腎細胞癌細胞(ACHN、769-P、786-O)相對於HK-2也有較高的表現。未來也將進一步探討BRAK/CXCL14和RhoA在腎細胞癌所扮演的角色,評估當標記(marker)的可能性,或能提供一新的治療方法,以提供醫療臨床上的應用。
Renal-cell carcinoma (RCC) is a very difficult tumor to treat, and response rates to biological therapies are less than 20%. The identification of various molecular and cellular markers has led to the development of novel therapies. We first used the microarray technology to screen the two different expression between RCC tumor sites and normal sites genes — BRAK/CXCL14 and RhoA, and then detected the expression of BRAK/CXCL14 and RhoA by RT-PCR, real-time PCR, Western blotting and immunohistochemistry. Compared with normal sites, our results showed that in the RCC tumor sites of 36 patients, BRAK/CXCL14 was down-regulated in gene and protein level 72.22% and 63.89%, respectively; in cell lines study, RCC cell lines (ACHN, A498, 769-P, 786-O) also had lower expression compared with normal proximal tubule cell line (HK-2). RhoA was up-regulated in gene and protein level 58.33% and 50.00%, respectively; RCC cell lines (ACHN, 769-P, 786-O) also had higher expression compared with HK-2. However, the function of these two genes still need to be further investigated. We expect that BRAK/CXCL14 and RhoA would play an important role in RCC carcinogenesis, and we hope to expand its application in the clinical medicine in the future.
英文摘要……………………………………………3
中文摘要……………………………………………4
緒論…………………………………………………5
研究目的……………………………………………17
實驗方法……………………………………………18
結果…………………………………………………32
討論…………………………………………………40
圖表…………………………………………………48
附圖…………………………………………………66
參考文獻……………………………………………68
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