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研究生:彭維榮
研究生(外文):Wei-jung Peng
論文名稱:樟芝保護肺細胞及預防動脈硬化機制之探討
論文名稱(外文):The effects of Antrodia camphorata on protecting lung cell and preventing atherosclerosis
指導教授:陳齊聖陳齊聖引用關係許游章
指導教授(外文):Chee-shan ChenYou-cheng Hseu
學位類別:碩士
校院名稱:朝陽科技大學
系所名稱:應用化學系碩士班
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:中文
論文頁數:95
中文關鍵詞:動脈粥狀硬化抗氧化MAP Kinases樟芝醱酵液(AC-10)香菸萃取液活性含氧物種
外文關鍵詞:MAP KinasesReactive oxygen species(ROS)AtherosclerosisFermnted culture broth of A.camphorata (AC-10)cigarette smoke extract (CSE)anti-oxidant
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第1部份

吸煙對人體健康的危害,主要是香煙中含有大量的自由基以及活性氧分子(ROS),導致人體內的抗氧化物質消耗而造成氧化傷害。近來有研究指出平滑肌細胞凋亡也是造成動脈粥狀硬化的原因之一。實驗中,以觀察 MAP Kinases 之蛋白活性為主。實驗中採用大鼠平滑肌細胞(A7r5)探討樟芝發酵液對於加入香煙萃取液之後是否具有保護效果能夠延緩其凋亡。在西方墨點法實驗中,發現香菸萃取液會活化 p-JNK、p-ERK、p-c-fos、p-MEK、p-p38、pro-casepase-8、p53、c-Jun..等蛋白之表現量增加,佐證了香煙萃取液會造成平滑肌細胞之損傷。在另一方面,也證明了樟芝醱酵液(AC-10)具有保護平滑肌細胞之效能。

第2部分

Antrodia camphorata 俗名稱為牛樟芝,為台灣特有的藥用真菌,其常應用於各種疾病的治療,具有抑制腫瘤細胞生長及具有抗氧化效果之化合物。吸煙會造成人體健康的危害,主要是香煙中含有大量的自由基以及活性含氧物種(reactive oxygen species, ROS),導致人體內的抗氧化物質消耗、最終造成氧化傷害。其中,香菸煙霧中含有大量之ROS,活性含氧物種,為自由基中最廣被為討論的一群,由於具有不成對電子, 所以反應性強並可引發一連串連鎖反應(chain- reaction),因而可進一步攻擊人體細胞組織,或產生二級次產物促使細胞結構與功能發生變化,對生物體造成傷害。實驗中,樟芝(Antrodia cinnamomea, AC) 具有抗氧化作用的功能,能預防來自氧化方面之傷害,保護肺細胞(MRC-5),減少來自於香菸萃取液之傷害。
第1部分

Smoking is dangerous for human bodies.The most important reason is cigarettes contain a plenty of free radical and ROS. Recently researches point out the cell apoptosis is the one reason of artherosclerosis. In this study, we examined the pathways for MAP Kinases(Mitogen Activated Protein Kinases)protein activation. In the experiment,the A7r5 from rats to talk about FCBA(AC-10) owns the protective effects and the ability of delaying aging after adding CSE .In the western blotting,we find CSE cause p-JNK、p-ERK、p-c fos、p-MEK、p-p38、pro-casepase-8、p53、 c-Jun... expression increasing,to prove the CSE cause the smooth muscle cell to apoptosis. The expression increasing just to prove it make damage to smooth muscle cell. Otherwise,that AC-10 has good at protecting on smooth muscel cell.

第2部分

The fruiting body of Antrodia camphorata is well known in Taiwan as “niu-chang chih” It has been used for many disease:the treatment of food and drug intoxication, abdominal pain, hypertension, skin itching and anti-tumor. Smoking is dangerous for human bodies.The most important reason is cigarettes contain a plenty of free radical and ROS.Reactive oxygen species (ROS) are discussed extensively in free radicals. Because of unpair electrons, ROS will have strong activation to induce a series chain-reaction,which can result to attack human cells and tissues or produce secondary substances to prompt cells structure and function changed. In the study,Antrodia camphorata owns the ability of protecting from oxidation and against cardiovascular,protecting the lung cell(MRC-5)to decrease damege from cigarettes smoke extract .
中文摘要………………………………………….………I
英文摘要………………………………………….………II
圖目錄………………………………………….……….....III

第一部份 香菸萃取液對平滑肌細胞之凋亡路徑探討
前言……………………………….………………………2-32
實驗架構…………………………………………………33
材料與方法………………………………………………34-49
結果與討論………………………………………………50-65
結論………………………………………………………66
第二部份 樟芝預防菸害對肺纖維母細胞之探討
摘要………………………………………………………68-69
前言………………………………………………………70-73
材料與方法………………………………………………74-80
結果與討論………………………………………………81-85
結論………………………………………………………86
總結………………………………………………………87
參考文獻…………………………………………………88-95
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