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研究生:徐聿
研究生(外文):Yu Hsu
論文名稱:靈芝、樟芝、裂褶菌多醣體對大白鼠自然殺手細胞與細胞素的影響
論文名稱(外文):The Influence of Natural Killer Cells Activity and Cytokine Production of Wistar Rats by Using Three Different β-glucan Extracted from Ganoderma lucidum, Antrodia camphorata, and Schizophyllum commune
指導教授:陳秀男陳秀男引用關係
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:漁業科學研究所
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:2006
畢業學年度:94
語文別:中文
論文頁數:81
中文關鍵詞:β-葡聚多醣體自然殺手細胞細胞素
外文關鍵詞:β-glucanNK CellCytokine
相關次數:
  • 被引用被引用:5
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  • 收藏至我的研究室書目清單書目收藏:1
本研究分別將靈芝、樟芝、裂褶菌三種不同菇蕈類所萃取的β-葡聚多醣體(β-glucan),以口服方式持續給予大白鼠一個月,並觀察對於大白鼠脾臟中自然殺手細胞毒殺能力的影響,以及檢測血液中細胞素的含量變化。結果發現靈芝、裂褶菌多醣體餵食組自然殺手細胞的毒殺能力均比對照組高且有顯著差異(P<0.05),而樟芝多醣體餵食組則在前三週顯著高於對照組,第四週則與對照組沒有顯著差異(P<0.05)。在測試血液中細胞素的實驗中,餵食三種不同多醣體均可顯著提升IFN-γ在血液中的含量(P<0.05);靈芝、裂褶菌多醣體餵食組血液中IL-2的含量顯著高於對照組(P<0.05),樟芝多醣體餵食組則與對照組沒有顯著差異(P<0.05)。靈芝、裂褶菌多醣體餵食組血液中IL-12的含量則分別在第6、第3天有對大值且顯著高於對照組(P<0.05),樟芝多醣體餵食組則與對照組沒有顯著差異。TNF-α在血液中的含量方面,靈芝、裂褶菌餵食組分別在第6、第10天顯著高於對照組(P<0.05),樟芝多醣體餵食組則與對照組沒有顯著差異。
In this study, Wistar rats were fed with three differentβ-glucan extracted from Ganoderma lucidum, Antrodia camphorata, and Schizophyllum commune. After 1 month experimental period, rats were sacrificed for the activity of natural killer cells and cytokine analysis.
The result demonstrated that the activity of natural killer cells in rats fedβ-glucan extracted from Ganoderma lucidum and Schizophyllum commune were significantly higher than control group(P<0.05). The activity of natural killer cells in rats fedβ-glucan extracted from Antrodia camphorata was significantly higher than control group in the 1st three weeks.
In addition, IFN-γof rats fed different β-glucan were significantly higher than control group(P<0.05). IL-2 of rats fedβ-glucan extracted from Ganoderma lucidum and Schizophyllum commune were significantly higher than control group(P<0.05). IL-2 of rats fedβ-glucan extracted from Antrodia camphorata had no significant(P<0.05)different with the control group. IL-12 of rats fedβ-glucan extracted from Ganoderma lucidum and Schizophyllum commune were significantly higher than control group(P<0.05)on the 6th and 3rd day respectively, and IL-12 of rats fed β-glucan extracted from Antrodia camphorata had no significant(P<0.05)different with the control group. TNF-αof rats fedβ-glucan extracted from Ganoderma lucidum and Schizophyllum commune were significantly higher compared to that of the control rats(P<0.05)on the 6th and 10th day respectively, and that of rats fed β-glucan extracted from Antrodia camphorata had no significant(P<0.05)different with the control group.
目錄
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謝誌----------------------------------------------------------------------------------I
目錄---------------------------------------------------------------------------------II
圖目錄-----------------------------------------------------------------------------III
中文摘要---------------------------------------------------------------------------1
英文摘要---------------------------------------------------------------------------2
前言---------------------------------------------------------------------------------4
實驗目的-------------------------------------------------------------------------20
材料方法-------------------------------------------------------------------------21
實驗結果-------------------------------------------------------------------------33
討論-------------------------------------------------------------------------------40
參考文獻-------------------------------------------------------------------------48
附件-------------------------------------------------------------------------------59










圖目錄
頁次
圖一、餵食菇蕈多醣體三天脾臟自然殺手細胞的活性變化。
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圖二、餵食菇蕈多醣體三天血液中IFN-γ的含量變化。
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圖三、葡萄糖濃度之吸光值標準曲線。
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圖四、自然殺手細胞純度檢測。
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圖五、以流式細胞儀檢測自然殺手細胞的活性。
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圖六、不同種類多醣體對於自然殺手細胞活性的影響。
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圖七、不同濃度之IFN-γ標準品作出之標準曲線。
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圖八、不同時間點IFN-γ的含量。
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圖九、不同濃度之IL-2標準品作出之標準曲線。
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圖十、不同時間點IL-2的含量。
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圖十一、不同濃度之IL-12標準品作出之標準曲線。
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圖十二、不同時間點IL-12的含量。
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圖十三、不同濃度之TNF-α標準品作出之標準曲線。
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圖十四、不同時間點TNF-α的含量。
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圖十五、對照組各同時間點cytokine的含量變化。
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圖十六、靈芝多醣處理組各同時間點cytokine的含量變化。
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圖十七、樟芝多醣處理組各同時間點cytokine的含量變化。
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圖十八、裂褶菌多醣處理組各同時間點cytokine的含量變化。
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丁懷謙(2000)食藥用菇多醣體之免疫生理活性。食品工業第32 卷第5 期:28-42。
王貞文(2000)靈芝對糖尿病患之輔助治療療效。國立中興大學生命科學院碩士在職專班碩士論文。
李一宏(2002)樟芝菌絲體之培養及其多醣體抗乙型肝炎病毒活性評估。中國醫藥學院中國藥學研究所博士論文。
吳政翰(2004)β-葡聚多醣體隊小白鼠吞噬細胞之吞噬作用和細胞素的影響。國立台灣大學動物學研究所碩士論文。
林欣穎(2002)裂褶菌多醣體的濃度分析以及其對小白鼠之抗氧化活性測試。國立台灣大學動物學研究所碩士論文。
陳妙齡(2001)以松杉靈芝餵食BALB/c鼠探討腹腔免疫反應的功能評估指標。國立台灣大學農業化學研究所碩士論文。
張毅偉(2003)靈芝中具β-(1→6)分支之(1→3)-β-D-聚葡萄糖的性質與檢測。國立臺灣大學食品科技研究所博士論文。
賀駿業 (2003) 靈芝多醣體最佳培養條件及對小白鼠之免疫反應研究。國立台灣大學動物學研究所碩士論文。
黃鈴娟(2000)樟芝與姬松茸之抗氧化性質及其多醣組成分析。國立中興大學食品科學研究所博士論文。
蔡淑瑤(2002)靈芝與柳松菇之抗氧化性質和其對腫瘤細胞之毒性及柳松菇之抗致突變性質。國立中興大學食品科學研究所碩士論文。
鄭惠華、董一致、董大成 (1985) 人工栽培之靈芝萃取物之抗腫瘤作用:口服靈芝萃取液對人體內T 細胞亞群之影響。中華癌醫會誌1: 10。
戴宇昀(2001)樟芝菌絲體與子實體對四氯化碳及酒精誘導之慢性及急性肝損傷之保肝功能評估。國立中興大學食品科學研究所碩士論文。
謝嘉美(2004)裂褶菌多醣及其生理活性之研究。南台科技大學生物科技研究所碩士論文。
賴慶亮譯。水野卓、川合正允(1997)菇類的化學.生化學。國立編譯館。
菊本昭一、宮島徹、吉積智司、藤本紫郎、木村惠太郎 (1970) 農化誌 44: 337。
Abbas, A. K., Lichtman A. H., Pober J. S. (2003) Cellular and Molecular Immunology. Philadelphia, Pennsylvania.
Ainsworth, G. C., Sparrow, F. K., Sussman, A. S. (1973) The Fungi. Vol. IV. B. A Taxonomic Review with Keys: Basidiomycetes and Lower Fungi. Academic Press, Inc. New York.
Ataoglu, H., Dogan, M. D., Mustafa, F., Akarsu, E. S. (2000) Candida albicans and Saccharomyces cerevisiae cell wall mannans produce fever in rats: role of nitric oxide and cytokines. Life Science 67(18): 2247- 2256
Black, P. L., McKinnon, K. M., Wooden, S. L., Ussery, M. A. (1996) Antiviral activity of biological response modifiers in a murine model of AIDS. Requirement for augmentation of natural killer cell activity and synergy with oral azt. International Journal of Immunopharmacology 18(11): 633- 650.
Biron, C. A. (1997) Activation and function of natural killer cells responses during viral infection. Current Opinion in Immunology 9: 24- 34.
Cheng, H. H., Hsieh, K. H., Tung, Y. C., Tung, T. C. (1988) Effect of Ganoderma lucidum extract on interleukin-2 production in mice. Journal of Chinese Oncology Society 4:13- 22.
Chihara, G., Hamuro, J., Maeda, Y. Y., Shiio, T., Suga, T., Takasuka, N., Sasaki, T. (1987) Antitumor and metastasis-inhibitory activities of lentinan as an immunomodulator: An overview. Cancer Detection and Prevention Supplement I: 423-443.
Condevaux, F., Guichard, J., Forichon, A., Aujoulat, M., Descotes, J. (2001) Compared effects of morphine and nickel chloride on NK cell activity in vitro in rats and monkeys. Journal of Applied Toxicology 21: 431- 434.
Demetrio, D., Luigi, C., Elena, L., Anna, C., Lucia, P. M., Franco, C., Guiseppe, T. (1997) Interleukin-6 production by human monocytes stimulated with Cryptococcus neoformans components. Infection and Immunity 65 (6): 2454- 2456.
Farrar W.L., Birchenall-Sparks M.C., Young H.B. (1986) Interleukin-2 induction of interferon-γ mRNA synthesis. Journal of Immunology 137: 3836- 3840.
Fujimiya, Y., Suzuki, Y., Oshiman, K., Kobori, H., Moriguchi, K.,Nakashima, H., Matumoto, Y., Takahara, S., Ebina, T., Katakura,R. (1998) Selective tumoricidal effect of soluble proteoglucan extracted from the basidiomycete, Agaricus blazei Murill, mediated via natura killer cell activation and apoptosis. Cancer Immunol Immunother 46: 147-159.
Gately, M. K., Renzetti, L. M., Magram, J., Stern, A. S., Adorini L., Gubler, U., Presky, D. H. (1998) The interleukin-12/interleukin-12-receptor system: role in normal and pathologic immune responses. Annual Review of Immunology 16: 495- 521.
Goldsby, R. A., Kindt, T. J., Osborne, B. A. (2000) Kuby Immunology (4th) Freeman and Company, New York.
Grattan, C. E., Dawn, G., Gibbs, S., Francis, D. M. (2003) Blood basophil numbers in chronic ordinary urticaria and healthy controls: diurnal variation, influence of loratadine and prednisolone and relationship to disease activity. Clinical and Experimental Allergy 33: 337- 341
Hoshi, H., Yagi, Y., Iijima, H., Matsunaga, K., Ishihara, Y., Yasuharat, T. (2005) Isolation and characterization of a novel immunomodulatory r-glucan-protein complex from the mycelium of Tricholoma matsutake in basidiomycetes. Journal of Agricultural and Food Chemistry 53: 8948- 8956
Ikekawa, T., Nakanishi, M., Uehara, U., Chihara, G., Fukuoka, F. (1968) Antitumor action of some basidiomycetes, especially Phllinus inteus. Japanese Journal of Cancer Research 59 (2): 155- 157.
Janeway, C. A., Travers, P., Walport, M., Capra, J. D. (1999) Immunobiology (4th), Garland Publishing, New York.
Kamiryo, Y., Yajima, T., Saito, K., Nishimura, H.,Fushimi, T.,Ohshima, Y., Tsukamoto, Y., Naito, S., Yoshikai, Y. (2005) Soluble branched (1,4)-b-D-glucans from Acetobacter species enhance antitumor activities against MHC class I-negative and -positive malignant melanoma through augmented NK activity and cytotoxic T-cell response. International Journal of Cancer 115(5): 769-776.
Kasaian, M. T., Whitters, M. J., Carter, L. L., Lowe, L. D., Jussif, J. M., Deng, B., Johnson, K. A., Witek, J. S., Senices, M., Konz, R. F., Wurster, A. L., Donaldson, D. D., Collins, M., Young, D. A., Grusby, M. J. (2002) IL-21 limits NK cell responses and promotes antigen-specific T cell activation: a mediator of the transition from innate to adaptive immunity. Immunity 16: 559-569.
Kägi, D., Ledermann, B., Bürki. K., Zinkernagel, R. M., Hengartner, H. (1996) Molecular mechanisms of lymphocyte-mediated cytotoxicity and their role in immunological protection and pathogenesis in vivo. Annual Review of Immunology 14: 207-232.
Khatri, V. P., Fehniger, T. A., Baiocchi, R. A., Yu, F., Shah, M. H., Schiller, D. S., Gould, M., Gazzinelli, R. T., Bernstein, Z. P., Caligiuri, M. A. (1998) Ultra low dose interleukin-2 therapy promotes a type 1 cytokine profile in vivo in patients with AIDS and AIDS-associated malignancies. The Journal of Clinical Investigation 101: 1373-1378.
Kiessling, R., Klein, E., Wigzell, H. (1975) "Natural" killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype. European Journal of Immunology 5 :112- 117.
Kikuchi,T., Ohno, N., Ohno, T. (2002) Maturation of dendritic cells induced by Candida beta-D-glucan. International Immunopharmacology 2(10): 1503-1508.
Lieu, C. H., Lee, S. S., Wang, S.Y. (1992) The effect of Ganoderma lucidum on induction of differentiation in leukemic U937 cells. Anticancer Research 12: 1211-1216.
Lin , J. M., Lin, C. C., Chiu, H. F., Yang, J. J., Lee, S. G. (1993) Evaluation of the anti-inflammatory and liver-productive effects of Aneectochilus formosanus, Ganoderma lucidum and Gynostemma pentaphyllum in rat. The American Journal of Chinese Medicine 21: 59- 69.
Mayell M. (2001) Maitake extracts and their therapeutic potential. Alternative Medicine Review 6(1): 48-60.
Minato, N., Reid, L., Bloom, B. R. (1981) On the heterogeneity ofmurine natural killer cells. The Journal of Experimental Medicine 154: 750-762.
Mizuno, T., Kinoshita, T., Zhuang, C., Ito, H., Mayuzumi, Y. (1995) Antitumor-active heteroglycans from niohshimeji mushroom,Tricholoma giganteum. Bioscience, Biotechnology, and Biochemistry 59: 568-71.
Nakagawa, Y., Ohno, N., Murai, T. (2003) Suppression by Candida albicans β-glucan of cytokine release from activated human monocytes and from T cell in the presence of monocytes. The Journal of Infectious Disease 187(4): 710-713.
Ohno, N., Uchiyama, M., Tsuzuka, K., Miura, N. N., Adachi, Y., Aizawa, M. W., Tamura, H., Tanaka, S., Yadomae, T. (1999) Solubilization of yeast cell-wallβ-(1,3)-d-glucan by sodium hypochlorite oxidation and dimethyl sulfoxide extration. Carbohydrate Research 316:161-172.
Perussia, B. (1996) The cytokine profile of resting and activated NK cells. Methods 9(2): 370- 378.
Romani, L., Mencacci, A., Cenci, E., Spaccapelo, R., Toniatti, C., Puccetti, P., Bistoni, F., Poli, V. (1996) Impaired neutrophil response and CD4+ T helper cell 1 development in interleukin 6-deficient mice infected with Candida albicans. The Journal of Experimental Medicine 183 (4): 1345- 1355.
Sakurai, T., Ohno, N., Yadomae, T. (1996) Effects of fungal beta-glucan and interferon-gamma on the secetory functions of murine alveolar macrophage. Journal of Leukocyte Biology 60(1): 118- 124.
Sakurai, T., Ohno, N., Yadomae, T. (1992) Therapeutic intervention with complement and beta-glucan in cancer. Immunopharmacology 42: 61- 47.
Seaman W. E. (2000) Natural killer cells and natural killer T cells. Arthritis and Rheumatism 43(6): 1204-1217.
Sietsma J.H., Wessels J.G. (1977) Chemical analysis of the hyphal wall of Schizophyllum commune. Biochimica et biophysica acta 496(1): 225- 39.
Tada, H., Eiji, N., Hidetoshi, S., Toshihiko, W., Takeshi, M., Tatsuji, M., Ohno, N., Tamura, H., Shibata, K. I., Akashi, S., Miyake, K., Sugawara, S., Takada, H. (2002) Saccharomyces cerevisiae- and Candida albicans-derived mannan induced production of tumor necrosis factor alpha by human monocytes in CD14- and tool-like receptor 4-dependent manner. Microbiology and Immunology 46(7): 503- 512.
Trinchieri G. (2003) Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nature Review Immunology 3(2): 133-146.
Trinchieri G. (1995) Interleukin-12: a proinflammatory cytokine with immunoregulatory functions that bridge innate resistance and antigen-specific adaptive immunity. Annual Review of Immunology 13:251-276.
Tripp, C. S., Wolf, S. F., Unanue, E. R. (1993) Interleukin 12 and tumor necrosis factor a are costimulators of interferon g production by natural killer cells insevere combined immunodeficiency mice with listeriosis, and interleukin 10 is a physiologic antagonist. Proceedings of the National Academy of Sciences of the United States of America 90: 3725- 3729.
Wakshull, E., Brunke-Reese, D., Lindermuth, J., Fisette, L., Nathans, R.S., Crowley, J.J., Tufts, J.C., Zimmerman, J., Mackin, W., Adams, D.S. (1999) PGG-glucan, a soluble beta-(1,3)-glucan, enhances the oxidative burst response, microbicidal activity, and activates an NF-kappa B-like factor in human PMN: evidence for a glycosphingolipid beta-(1,3)-glucan receptor. Immunopharmacology 41(2): 89-107.
Wang, S. Y., Hsu, M. L., Hsu, H. C.,Tzeng, C. H., Lee, S. S., Shiao, M. S., Ho, C.K. (1997) The anti-tumor effect of Ganoderma lucidum is mediated by cytokines relased from activated macrophage and T lymphocytes. International Journal of Cancer 70: 699-705.
Won, S. J., Lin, M. T., Wu, W. L. (1992) Ganoderma tsugae mycelium enhances splenic natural killer cell activity and serum interferon production in mice. Japanese Journal of Pharmacology 59: 171-176.
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