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研究生:吳俊緯
研究生(外文):Wu Chunwei
論文名稱:樟芝水對大鼠的保肝功效
論文名稱(外文):The hepatoprotective effect of liquid extractive Antrodia camphorata for rats
指導教授:陳翼鵬
學位類別:碩士
校院名稱:南台科技大學
系所名稱:生物科技系
學門:生命科學學門
學類:生物科技學類
論文出版年:2006
畢業學年度:94
語文別:中文
論文頁數:53
中文關鍵詞:樟芝四氯化碳肝損傷
外文關鍵詞:Antrodia camphorataCCl4liver damage
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樟芝(Antrodia camphorata)為台灣特有種真菌,因只生長在台灣特有的牛樟樹中,數量稀少而珍貴,民間長久以來皆視為治療肝臟疾病之聖品。而肝炎及肝硬化等肝臟疾病久為十大死因之一,長期威脅著國人的健康,故開發治療肝病的藥物實為當務之急。本研究即以樟芝發酵液水萃後製成的樟芝水為材料,進行保肝功能成效的研究,期望能對國人健康有所貢獻。
實驗以Wistar大鼠為動物模式,隨機分為五組。A組:正常控制組(未經四氯化碳處理,其餘則皆經四氯化碳處理)。B組:負控制組(CCl4組)。C組:正對照組(Silymarin組)。D組:低劑量實驗組(樟芝水1X)。E組:高劑量實驗組(樟芝水5X)。實驗進行四週,結果顯示:在血清生化值方面,樟芝水實驗組(D、E組)的glutamyl oxaloacetic transaminase(GOT)和glutamyl pyrubic transaminase(GPT)與負控制組間的差異皆達到顯著水準(p < 0.05),且與正常控制組間沒有顯著差異,代表樟芝水可降低肝功能指數至與正常值接近。但兩組實驗組(樟芝水1X與樟芝水5X)間並無顯著差異,代表增加樟芝水劑量並無法增加功效。在triglyceride與cholesterol方面,樟芝水1X與5X兩組和負控制組間皆可達到顯著差異,表示樟芝水可有效降低血脂肪含量,但兩實驗組間的效果差異不顯著。在肝臟中的抗氧化效果部份,抗氧化物Glutathione(GSH)在所有各組間的含量均無顯著差異,但樟芝水可顯著提升抗氧化酵素superoxide dismutase(SOD)在肝臟中的活性,對於肝臟中catalase(CAT)的活性則略有提升效果,然而並未達到顯著水準。由以上結果可知,樟芝水對於四氯化碳所誘發的肝損傷具有保護功效,亦有降低血脂肪的效果。
Antrodia camphorata is a unique species of fungus that only growes on Cinnamomum kanehirai in Taiwan. It is recognized as traditional herb for its liver protective function in Taiwan aborigines. It has long been known that liver disease is one of the top ten killers in Taiwan. Therefore, it is important to develop medicine that curing liver diseases. The aim of this study was to investigate the hepatoprotective effect of liquid extractive Antrodia camphorate (LEAC), which is the water extract of solid form of Antrodia camphorata fermentation, and to see if the LEAC might help liver function.
The present study used Wistar rats as our study animal . Twenty rats were divided into five groups: Normal control (Background control no CCl4 was introduced), Negative control (CCl4 alone and feed only with solvent), Test group one [the CCl4+silymarin (positive drug control)], Test group two (CCl4+1X LEAC), and Test group three (CCl4+5X LEAC.)
Our results showed that the LEAC might have liver protective function as our data in test groups. Either 1X or 5X LEAC could reduce the liver damages that caused by CCl4. These results could be demonstrated by the significantly decreased serum levels of GOT and GPT after feeding 1X and 5X LEAC, LEAC can also reduce the content of triglyceride and cholesterol in serum. In addition, the antioxidant status in liver of rats were tested. The content of glutathione did not show significantly decrease among five groups, but feeding LEAC significantly restored the activities of antioxidant enzyme SOD. The activities of catalase rose after feeding LEAC, but the effect isn’t significant.
第一章 文獻回顧…………………………………………………………..………..1
  第一節 樟芝簡介……………………………………………………..………..1
  第二節 樟芝功效介紹………………………………………………..………..5
  第三節 肝損傷模式探討……………………………………………..……….11
  第四節 水飛薊素……………………………………………………..……….14
  第五節 實驗動機……………………………………………………..……….16
第二章 實驗材料與方法…………………………………………………..……….17
  第一節 實驗材料…………………………………………………….………..17
  第二節 實驗方法…………………………………………………….………..18
第三章 結果……………………………………………………………….………..22
  第一節 樟芝水對CCl4誘導肝損傷大鼠之體重變化……………..…………22
  第二節 樟芝水對CCl4誘導肝損傷大鼠血清生化值的影響……………..…23
  第三節 CCl4誘導肝損傷大鼠服用樟芝水後肝臟中的GSH含量變化……….26
  第四節 CCl4誘導肝損傷大鼠服用樟芝水後肝臟中SOD及CAT含量變化……26
第四章 討論………………………………………………………………………...28
  第一節 體重…………………………………………………………………...28
  第二節 肝功能指數…………………………………………………………...28
  第三節 血脂肪………………………………………………………………...30
  第四節 抗氧化物與抗氧化酵素……………………………………………...31
第五章 結論……………………………………………………………...………...34
第六章 參考文獻…………………………………………………………………..44
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