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研究生:戴佩敏
研究生(外文):Pei Min Dai
論文名稱:蛋白激酶C在NPC人類鼻咽癌細胞中對Nanog的調控作用
論文名稱(外文):Regulation of Nanog expression by protein kinase Cs in human NPC cell lines
指導教授:陳君侃陳君侃引用關係
指導教授(外文):J.K.Chen
學位類別:碩士
校院名稱:長庚大學
系所名稱:基礎醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2008
畢業學年度:96
論文頁數:57
中文關鍵詞:鼻咽癌幹細胞轉錄因子蛋白激酶C
外文關鍵詞:PKCNanogNasopharyngeal
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蛋白激酶C在哺乳動物組織至少已發現12種亞型,這些亞型不但在結構上有差異,在細胞的分佈、受質特異性及對活化訊號的敏感性都有所不同,它們也各自有其特殊的功能,某些特定亞型可參與調控分化、增殖與癌化過程。在幹細胞有表現的一些轉錄因子,如Nanog、Oct4、Sox2等,被發現與維持幹細胞self-renew及不分化或低分化的特性有關,這些因子在幹細胞中具有調控增殖與分化的功能,它們也會表現在某些癌細胞中。Nanog promoter片段上具有Oct4以及Sox2的結合位置,Oct4及Sox2可以協同作用的方式結合到Nanog promoter的位置並調控Nanog的表現。本研究以人類鼻咽癌細胞NPC 076 cell為模型,探討Nanog在此癌細胞株表現的調控狀況:首先先探討PKC與Nanog表現的關連性,發現抑制PKC可使Nanog mRNA及蛋白質的表現量上升,並進一步確認PKC-α這個亞型在NPC 076 cell具有調節Nanog表現的功能。另外,本研究亦進一步證明PKC對Nanog的調控可經由Oct4及Sox2而達成。
Protein kinase C (PKC) is a family of serine/threonine phosphotransferases. The family consists of at least 12 isozymes, which play central regulatory roles in a bewildering multitude of cellular processes, including proliferation, differentiation and tumorigenesis. Transcription factors Nanog, Sox2, and Oct4 have been shown to be essential for maintaining the pluripotency and self-renewing characteristics of the embryonic stem cells (ESCs). Moreover, Oct4 and Sox2 have been shown to bind to Nanog promoter region in stem cells, suggesting that Nanog transcription may be regulated through interactions with Oct4 and Sox2. Interestingly, these three stem cell factors have been found to be also expressed in some human cancer cell. In this study, the activity of three PKC isoforms (PKC-α、δ、ε) which are believed to be involved in the regulation of cell differentiation and proliferation were examined to see if they influence Nanog expression. Our results indicate that PKC-α can downregulate Nanog expression in human NPC cell line through downregulating Oct4 and Sox2.
指導教授推薦書……………………………………………………...…ii
口試委員會審定書……………………………………………………..iii
授權書.………………………………………………………………….iv誌謝………………………………………………………………….v
中文摘要……………………………………………………………….vi
英文摘要………………………………………………………………vii
目錄…………………………………………………………………….viii
第一章 背景…………………………………………………………1
第二章 論文研究目的………………………………………………..11
第三章 實驗方法與材料……………………………………………12
第四章 結果…………………………………………………….…....19
第五章 結論…………………………………………………………26
圖表……………………………………………………………………30
參考文獻……………………………………………………….……..39
附錄………………………………………………………………….44
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