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研究生:曾承煒
研究生(外文):Cheng-Wei Tzeng
論文名稱:樟芝固態發酵物抗四氯化碳誘發小鼠急慢性肝損傷之保肝活性及其品質評價
論文名稱(外文):Solid fermented Antrodia cinnamomea inhibit acute and chronic hepatic injury induced by carbon tetrachloride (CCl4) and quality assessment.
指導教授:顏銘宏顏銘宏引用關係
指導教授(外文):Ming-Hong Yen
學位類別:碩士
校院名稱:高雄醫學大學
系所名稱:天然藥物研究所
學門:醫藥衛生學門
學類:藥學學類
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:110
中文關鍵詞:樟芝固態發酵物抗肝炎抗氧化脂質過氧化肝臟保護活性
外文關鍵詞:Solid fermented Antrodia cinnamomeaanti-hepatitisAntioxidantLipid peroxidationHepatoprotective activity
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樟芝 (Antrodia cinnamomea)在台灣是一著名的民間藥物,屬台灣特有種真菌,只生長在台灣特有的牛樟樹木材上,且無法人工培養。樟芝具有多種的生理功效,包括抗氧化、抗癌及肝臟保護作用。先前之研究發現樟芝具有抗B型肝炎病毒之活性。本研究的目的是開發樟芝的藥物資源並評估樟芝固態發酵物及其酒精和熱水萃取物對四氯化碳所誘發之小鼠急性及慢性肝損傷之肝臟保護活性。在急性(進行三天實驗)肝損傷實驗方面:以胃管餵食樟芝固態發酵物原物料懸浮液及酒精和熱水萃取物溶液後,由血液生化檢查顯示血清中GOT、GPT及LDH的濃度與四氯化碳組比較均有明顯降低的情形;藥物組在實驗中顯現了明顯抑制因腹腔注射四氯化碳所產生的三氯化碳自由基 (CCl3.)對肝臟造成傷害所產生的脂質過氧化的情形,而且隨著藥物濃度的增加抑制效果也越明顯。在慢性(進行八週實驗)實驗方面:樟芝固態發酵物組可以明顯的降低血中GOT、GPT及LDH值,且肝臟脂質過氧化的情形也會被抑制,而熱水及酒精萃取物則可降低血中LDH的濃度。由以上實驗結果推測樟芝固態發酵物可能經由抗氧化及清除自由基而抑制體內急性及慢性化學性肝損傷的作用,而具保肝活性。
Antrodia cinnamomea, an endemic fungus in Taiwan, is well-known as a folk medicine, it grows only on the inner heartwood wall of the endemic evergreen Cinnamonum kanehirai and can not be cultivated. It has much physiological activities, including antioxidant, anticancer and hepatoprotective activity, and has active constituents that have anti-hepatitis B virus activity. The purpose of this research is to evaluate the hepatic protective effect of solid fermented A. cinnamomea against carbon tetrachloride (CCl4)-induced acute and chronic hepatic injury in vivo. In the acute liver injury experiment (go for three days):after oral administration of solid fermented A. cinnamomea raw material suspension and ethanol extract and hot water extract, respectively, the results showed a significant decrease of GOT, GPT and LDH levels in mice serum compare to the carbon tetrachloride group. The raw material group showed the significant inhibition of liver lipid peroxidation induced by the carbon trichloride radical. In the chronic liver injury experiment (go for eight weeks):raw material of solid fermented A. cinnamomea significantly decreased the level of serum GOT, GPT and LDH, and also inhibit liver lipid peroxidation. Ethanol and water extracts of solid fermented A. cinnamomea can decrease the blood LDH level significantly. These results demonstrated that solid fermented A. cinnamomea may exist hepatoprotective effect against acute and chronic chemical-induced heapatic injury in vivo, by mediating antioxidative and free radical scavenging activities.
誌謝 2
中文摘要 5
英文摘要 6
第一章 序論 8
第一節 前言 9
第二節 化學性肝炎的概說 12
第三節 樟芝 (Antrodia cinnamomea) 簡介 14
第四節 實驗動機 20
第二章 材料與方法 22
第一節 材料的製備 23
第二節 實驗動物 24
第三節 化學藥品試劑與器材 25
第四節 四氯化碳誘導小鼠急性肝炎的方法 27
第五節 四氯化碳誘導小鼠慢性肝炎的方法 29
第六節 血清肝功能生化指數的檢測 31
第七節 肝臟中Malondialdehyde (MDA)含量的測定 32
第八節 肝臟中Glutathione (GSH)含量的測定 36
第九節 肝臟中超氧化物歧化酵素 (SOD)活性的分析 40
第十節 肝臟中Catalase活性的分析 44
第十一節 多醣含量的分析 49
第十二節 HPLC的檢品製備 52
第十三節 統計分析 55
第三章 結果 56
第一節 血清生化值的比較 57
第二節 樟芝固態發酵物及其萃取物抑制脂質過氧化的效果 59
第三節 樟芝固態發酵物及其萃取物對肝臟中Glutathione的影響 61
第四節 樟芝固態發酵物及其萃取物對SOD的影響 63
第五節 樟芝固態發酵物及其萃取物對Catalase的影響 64
第六節 樟芝固態發酵物之品質評價 66
第四章 討論 70
第五章 結論 78
第六章 參考文獻 80
圖 85
表 105
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