臺灣博碩士論文加值系統

國內癌症死亡的排名，肺癌及肝癌位居一、二位。有鑑於此；我們建立肺癌及肝癌的小鼠動物模式，以固體中藥渣培養樟芝及蟲草菌絲體，利用其萃取液作為肺癌及肝癌的治療，期望帶來新的治療契機。
肺癌模式方面：以中藥渣所培養的蟲草菌絲體萃取液（CS-J190），治療人類肺腺癌腫瘤，利用人類肺腺癌腫瘤細胞-A549來建立動物模式。 除了皮下腫瘤模式外；並建立產生原位肺癌動物模式，及肺腺癌末期併發所產生的腹水（Ascites）動物模式。在皮下腫瘤方面：每天餵食200mg /隻；連續49天的劑量下，其皮下肺腺癌腫瘤體積生長較小
（P＝0.0383），且轉移至肺臟產生產生腫瘤節結（colonies）的數量較少（P＝0.0386）。相同劑量下，連續60天餵食該萃取液，在肺臟產生腫瘤節結（colonies）的數量受到抑制（P＝0.0001）。在腹水模式中，以萃取液連續餵食14天，其腹腔積水的情形獲得改善（平均腹水1.68ml降至0.54ml）。肝癌模式方面：以固體中藥渣所培養的樟芝菌絲體萃取液（AC-J20），治療人類肝癌細胞-HepG2腫瘤，皮下肝癌腫瘤生長之小鼠，以400mg /隻（200mg /次，每天2次），連續餵食14天，腫瘤明顯較小（P＝0.0441），小鼠存活率延長約70 %。小鼠皮下腫瘤免疫染色切片結果證實，該萃取液對於人類肝癌細胞（HepG2）有抑制增生（Anti-proliferation）的功效。
我們實驗證明，在小鼠動物模式中，利用中藥渣培養樟芝菌絲體萃取液可以有效抑制肝癌生長；而固體中藥渣培養蟲草菌絲體萃取液則能有效抑制肺癌生長，並且均能延長小鼠存活率達70 %以上。此研究的發現，期望對癌症中藥臨床研究有更近一步的幫助。

The rank of the death in Taiwan on lung cancer and liver cancer is the first and the second one, respectively. In this study we set up the mice animal model for therapy of lung cancer and liver cancer. The cultures of Antrodia camphorata and Cordyceps sp. were inoculated on the solid state of Chinese herbal substrates. The mycelia were harvested and extracted with 95 ％ alcohol. In lung cancer model: the extract of Cordyceps sp. mycelia (CS-J190) was applied to the NOD-SCID mice（non-obese diabetic-severe combined immunodeficiency）after treating with lung adenocarcinoma cell of human (A549) for 49 days and lung cancer carcinoma in situ for 60 days by feeding 200 mg/day of extract. The result indicates that adenocarcinoma subcutaneous tumour volume is decreased (P =0.0383) and number of lung metastatic tumor colonies the treatment was lower than control group (P =0.0386). In the in situ model：the lung tumor colonies the treatment was lower than control group (P =0.0001). In the ascites model, ascites of treatment group was improved on days 14 (from volume 1.68ml dropped to 0.54ml). In liver cancer model, the extract of A. camphorata mycelia (AC-J20) was applied to the NOD SCID mice after inoculating hepatoma cell (HepG2) by feeding with 400 mg/day (200 mg/time, twice a day). The result on days 14 shown that the tumor size was obviously decreased (P=0.0441), and the survival rate prolonged up to 70%. The mechanism to reduce tumor volume results from immunochemistry staining has shown the extract of A. camphorata can inhibit the proliferation of HepG2 (Anti-proliferation). The results demonstrate that the extract of A. camphorata mycelia growing in Chinese herbal substrate can effectively suppress the proliferation of liver cancer, while the extract of Cordyceps sp. substrate can suppress the lung cancer effectively. Both increases the mice's survival rate may be help of the clinical trial in future.

中文摘要 ……………………………………………………………………………Ⅳ
英文摘要 ……………………………………………………………………………Ⅴ
誌謝………………………………………………………………………………… Ⅵ
目次 …………………………………………………………………………………Ⅶ
圖目錄……………………………………………………………………………… Ⅷ.Ⅸ.Ⅹ
表目錄……………………………………………………………………………… Ⅺ
第一章 前言 …………………………………………………………………………1
第二章 文獻回顧 ……………………………………………………………………2
2.1 菇類與真菌簡介…………………………………………………………………2
2.2 食藥用菇概述……………………………………………………………………6
2.3 樟芝簡介 ………………………………………………………………………10
2.4 蟲草簡介 ………………………………………………………………………16
2.5 液體培養與固體培養技術簡介 ………………………………………………19
2.6 肺癌與肝癌簡介 ………………………………………………………………20
第三章 材料與方法
3.1 實驗材料 ………………………………………………………………………22
3.2 實驗方法 ………………………………………………………………………26
第四章 結果與討論
4.1 SCID mice皮下肝癌腫瘤誘發實驗 …………………………………………42
4.2 SCID mice皮下肺癌腫瘤誘發實驗 …………………………………………45
4.3 SCID mice原位肺癌腫瘤誘發實驗 …………………………………………48
4.4 SCID mice肺癌病發腹腔積水誘發實驗 ……………………………………51
第五章 結論 ………………………………………………………………………53
第六章 參考文獻 ………………………………………………………………… 55

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