(100.24.209.47) 您好!臺灣時間:2019/08/26 10:23
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
本論文永久網址: 
line
研究生:林雅慧
研究生(外文):Ya-Hui Lin
論文名稱:以固體中藥渣培養樟芝及蟲草作為治療人類肝癌及肺癌的動物模式之研究
論文名稱(外文):Study on animal model of human lung cancer and liver cancer treated with extracts of Chinese herbal substrates through solid stage fermentation by Antrodia camphorata and Cordyceps sp.
指導教授:陳啟楨
指導教授(外文):Chee-Jen Chen
學位類別:碩士
校院名稱:南台科技大學
系所名稱:生物科技系
學門:生命科學學門
學類:生物科技學類
論文出版年:2008
畢業學年度:96
語文別:中文
論文頁數:82
中文關鍵詞:樟芝蟲草皮下腫瘤模式原位肺癌動物模式腹水動物模式腫瘤節結抑制增生
外文關鍵詞:Antrodia camphorateCordyceps sp.Chinese herbal substratesmyceliaNOD SCID miceadenocarcinomaproliferation
相關次數:
  • 被引用被引用:4
  • 點閱點閱:1939
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:659
  • 收藏至我的研究室書目清單書目收藏:4
國內癌症死亡的排名,肺癌及肝癌位居一、二位。有鑑於此;我們建立肺癌及肝癌的小鼠動物模式,以固體中藥渣培養樟芝及蟲草菌絲體,利用其萃取液作為肺癌及肝癌的治療,期望帶來新的治療契機。
肺癌模式方面:以中藥渣所培養的蟲草菌絲體萃取液(CS-J190),治療人類肺腺癌腫瘤,利用人類肺腺癌腫瘤細胞-A549來建立動物模式。 除了皮下腫瘤模式外;並建立產生原位肺癌動物模式,及肺腺癌末期併發所產生的腹水(Ascites)動物模式。在皮下腫瘤方面:每天餵食200mg /隻;連續49天的劑量下,其皮下肺腺癌腫瘤體積生長較小
(P=0.0383),且轉移至肺臟產生產生腫瘤節結(colonies)的數量較少(P=0.0386)。相同劑量下,連續60天餵食該萃取液,在肺臟產生腫瘤節結(colonies)的數量受到抑制(P=0.0001)。在腹水模式中,以萃取液連續餵食14天,其腹腔積水的情形獲得改善(平均腹水1.68ml降至0.54ml)。肝癌模式方面:以固體中藥渣所培養的樟芝菌絲體萃取液(AC-J20),治療人類肝癌細胞-HepG2腫瘤,皮下肝癌腫瘤生長之小鼠,以400mg /隻(200mg /次,每天2次),連續餵食14天,腫瘤明顯較小(P=0.0441),小鼠存活率延長約70 %。小鼠皮下腫瘤免疫染色切片結果證實,該萃取液對於人類肝癌細胞(HepG2)有抑制增生(Anti-proliferation)的功效。
我們實驗證明,在小鼠動物模式中,利用中藥渣培養樟芝菌絲體萃取液可以有效抑制肝癌生長;而固體中藥渣培養蟲草菌絲體萃取液則能有效抑制肺癌生長,並且均能延長小鼠存活率達70 %以上。此研究的發現,期望對癌症中藥臨床研究有更近一步的幫助。
The rank of the death in Taiwan on lung cancer and liver cancer is the first and the second one, respectively. In this study we set up the mice animal model for therapy of lung cancer and liver cancer. The cultures of Antrodia camphorata and Cordyceps sp. were inoculated on the solid state of Chinese herbal substrates. The mycelia were harvested and extracted with 95 % alcohol. In lung cancer model: the extract of Cordyceps sp. mycelia (CS-J190) was applied to the NOD-SCID mice(non-obese diabetic-severe combined immunodeficiency)after treating with lung adenocarcinoma cell of human (A549) for 49 days and lung cancer carcinoma in situ for 60 days by feeding 200 mg/day of extract. The result indicates that adenocarcinoma subcutaneous tumour volume is decreased (P =0.0383) and number of lung metastatic tumor colonies the treatment was lower than control group (P =0.0386). In the in situ model:the lung tumor colonies the treatment was lower than control group (P =0.0001). In the ascites model, ascites of treatment group was improved on days 14 (from volume 1.68ml dropped to 0.54ml). In liver cancer model, the extract of A. camphorata mycelia (AC-J20) was applied to the NOD SCID mice after inoculating hepatoma cell (HepG2) by feeding with 400 mg/day (200 mg/time, twice a day). The result on days 14 shown that the tumor size was obviously decreased (P=0.0441), and the survival rate prolonged up to 70%. The mechanism to reduce tumor volume results from immunochemistry staining has shown the extract of A. camphorata can inhibit the proliferation of HepG2 (Anti-proliferation). The results demonstrate that the extract of A. camphorata mycelia growing in Chinese herbal substrate can effectively suppress the proliferation of liver cancer, while the extract of Cordyceps sp. substrate can suppress the lung cancer effectively. Both increases the mice's survival rate may be help of the clinical trial in future.
中文摘要 ……………………………………………………………………………Ⅳ
英文摘要 ……………………………………………………………………………Ⅴ
誌謝………………………………………………………………………………… Ⅵ
目次 …………………………………………………………………………………Ⅶ
圖目錄……………………………………………………………………………… Ⅷ.Ⅸ.Ⅹ
表目錄……………………………………………………………………………… Ⅺ
第一章 前言 …………………………………………………………………………1
第二章 文獻回顧 ……………………………………………………………………2
2.1 菇類與真菌簡介…………………………………………………………………2
2.2 食藥用菇概述……………………………………………………………………6
2.3 樟芝簡介 ………………………………………………………………………10
2.4 蟲草簡介 ………………………………………………………………………16
2.5 液體培養與固體培養技術簡介 ………………………………………………19
2.6 肺癌與肝癌簡介 ………………………………………………………………20
第三章 材料與方法
3.1 實驗材料 ………………………………………………………………………22
3.2 實驗方法 ………………………………………………………………………26
第四章 結果與討論
4.1 SCID mice皮下肝癌腫瘤誘發實驗 …………………………………………42
4.2 SCID mice皮下肺癌腫瘤誘發實驗 …………………………………………45
4.3 SCID mice原位肺癌腫瘤誘發實驗 …………………………………………48
4.4 SCID mice肺癌病發腹腔積水誘發實驗 ……………………………………51
第五章 結論 ………………………………………………………………………53
第六章 參考文獻 ………………………………………………………………… 55
劉業經。台灣重要樹木彩色圖治。1970。國立中興大學,台灣省林務局合作研究報告

許瑞祥。人工培養冬蟲夏草屬之性質及其代謝物之探討。1984。國立台灣大學農業化學研究所碩士論文

蘇慶華。新屬植生蟲草(Phytocordyceps)型態遺傳及生化之研究。1985。台灣大學農業化學研究所博士論文

卯曉蘭。中國野生食用真菌種類及生態特性。1988。真菌學報27

劉國柱。現代科學看靈芝。1990。雙利實業有限公司

高曉薇。台灣靈芝屬新種樟芝之三帖類成份研究。1992。台北醫學院天然物醫物研究所碩士論文

王伯徹、陳啟楨。常見食藥用菇介紹。1994。食品工業發展研究所

王國棟。冬蟲夏草類生態、培殖、應用。1995。科學技術文獻出版社

浦蟄龍、李增智。昆蟲真菌學。1996。安徽科學技術出版社

張佩靖。人工培養冬蟲夏草菌絲體對大白鼠血管作用之探討。1996國立陽明大學藥理研究所碩士論文

賴慶亮譯。水野卓、川合正允原著。菇類的化學.生化學。1997。國立編譯館。

簡秋源、姜宏哲、陳淑真。牛樟菇培養性狀及其三帖類成分分析之研究。1997。林業叢刊第72號

王伯徹、陳啟楨、華傑。食藥用菇的栽培與應用。1998。食品工業發展研究所

劉昌峰。探討不同培養基組成對光合菌Rhodobacter sphaeroides生產Coenzyme Q10 之研究。2000。中央大學化學工程學研究所碩士論文

黃鈴娟。樟芝與姬松茸之抗氧化性質及其多醣組成分析。2000。國立中興大學食品科學系碩士論文

徐敬衡、文榮輝、賴秋梅、翁偉恆。利深層發酵生產樟芝菌絲體及抗菌研究。2000。第15屆全國技術及職業教育研討會論文集

黃惠琴。樟芝菌絲體深層培養之研究。2001。東海大學化學工程研究所碩士論文

陳清農、許勝傑、藍明煌。樟芝固體栽培其生物活性研究。2001。中華真菌學會會刊16(1,2)

陳勁初2、呂鋒洲。靈芝之王:台灣樟芝。2001a。元氣齋出版社

陳勁初2、林文鑫、陳清農、許勝傑、黃仕政、陳炎鍊。台灣特有真菌-樟芝菌絲體之開發。2001b。中華真菌學會會刊 16:7-22

嚴貴榮。樟芝對SZT誘發高血糖鼠血醣調節與抗氧化之影響。2001。輔仁大學食品營養學系碩士論文

陳啟楨、蘇慶華、藍明煌。樟芝固體栽培其生物活性研究。2001。中華真菌學會會刊

蘇慶華。健康的守護神-國寶樟芝。2002。愛克思文化事業有限公司

劉俊仁。中草藥抗癌機制研究:(壹)黃芩素及黃芩對血管新生作用之影響及其機制探討;(貳):樟芝活性多醣體之生物活性分析及其經由免疫調節抑制腫瘤生長之研究。2002。台灣國立大學生物化學暨分子生物學研究所博士論文

陳永佳。冬蟲夏草刺激小鼠萊氏腫瘤細胞類固醇生成機制之探討。2002。國立成功大學細胞生物與解剖所研究所碩士論文

李宛蓁。樟芝菌絲體培養與生理活性成分生成之研究。2003。私立東海大學化學工程研究所碩士論文

宋祖瑩。樟芝深層培養液抗氧化及抗腫瘤活性之研究。2003。國立中興大學食品科學系博士論文

謝政蓉。E1B缺失性腺病毒對膀胱癌和肝癌的基因治療。2003。國立成功大學基礎醫學研究所博士論文

盧祉彤。牛樟芝菌絲體對腫瘤細胞的影響。2003。南台科技大學生物科技研究所碩士論文

林清淵。冬蟲夏草是蟲?是草?科學發展期刊。2003年4月,364期

陳啟楨。菇菇的世界。2004a。偉銘圖書股份有限公司

陳啟楨。藥食兩用的聖品-黑木耳。2004b。靈芝文摘第三集

方宋齡。蟲草素對雄性小鼠萊氏細胞固醇類生成之影響。2004。國立成功大學細胞生物與解剖所研究所碩士論文

沈坤德。真菌幾丁寡糖之抑癌潛能評估與積轉之探討。2004。國立成功大學環境醫學研究所碩士論文

齊藤隆。抗癌成功宣言。2004。鼎樂科技藝術有限公司

高怡蜓。以蛋白質體學技術研究蟲草屬分離菌株蛋白質圖譜。2005。南台科技大學生物科技研究所碩士論文

李佳安。桑黃固體栽培及其生物活性之探討。2005。南台科技大學生物科技研究所碩士論文

陳啟楨。金菇抗癌。2006。元氣齋出版社

潘博雄。蟲草素對MA-10小鼠萊氏腫瘤細胞固醇類生成之機制探討。2007。國立成功大學細胞生物與解剖所研究所碩士論文

黃美雪、黃良日、邱志明、王依華。建立保健食品及藥用菇菌產品品質之標準化(以牛樟菇為例)。2007。林業研究專訊 14(4)78:41-42

Bendrik C, Robertson J, Gauldie J, Dabrosin C. 2008. Gene transfer of matrix metalloproteinase-9 induces tumor regression of breast cancer in vivo. Cancer Res 68(9):3405-3412.
Breene WM. 1990 Nutritional and medical value of specialty mishrooms. J Food Prot 53:883-894
Chang HL,Chao GR,Chen CC,Mau JL. 2001. Non-volatile taste components of Agarius blazei,Antrodia camphorata and Cordyceps militaria mycelia. Food Chem 74:203-207
Chen CC, Liu YW, Ker YB, Wu YY, Lai EY, Chyau CC, Hseu TH, Peng RY. 2007. Chemical characterization and anti-inflammatory effect of polysaccharides fractionated from submerge-cultured Antrodia camphorata mycelia. J Agric Food Chem 55(13):5007-5012.
Chen YJ, Cheng PC, Lin CN, Liao HF, Chen YY, Chen CC, Lee KM. 2008. Polysaccharides from Antrodia camphorata mycelia extracts possess immunomodulatory activity and inhibits infection of Schistosoma mansoni. Int Immunopharmacol 8(3):458-467.
Cherng IH, Chiang HC. 1995 Three new triterpenoids from Antrodia cinnamomea. J Nat Prod 58:1655-1661
Cherng IH, Wu DP,Chiang HC. 1996 Triterpenoids from Antrodia cinnamomea. Phytochem 41(1):263-267
Cho HJ, Cho JY, Rhee MH, Park HJ. 2007. Cordycepin (3'-deoxyadenosine) inhibits human platelet aggregation in a cyclic AMP- and cyclic GMP-dependent manner. Eur J Pharmacol 558(1-3):43-51.
Hagiwara SY, Takahashi M, Shen Y, Kaihou S, Tomiyama T, Yazawa M, Tamai Y, Sin Y, Kazusaka A, Terazawa M. 2005. A phytochemical in the edible Tamogi-take mushroom (Pleurotus cornucopiae), D-mannitol, inhibits ACE activity and lowers the blood pressure of spontaneously hypertensive rats. Biosci Biotechnol Biochem 69(8):1603-1605.
Hikino H, Ishiyama M, Suzuki Y, Konno C. 1989. Mechanisms of hypoglycemic activity of ganoderan B: a glycan of Ganoderma lucidum fruit bodies. Planta Med 55(5):423-428.
Hseu YC, Chen SC, Chen HC, Liao JW, Yang HL. 2008a. Antrodia camphorata inhibits proliferation of human breast cancer cells in vitro and in vivo. Food Chem Toxicol.
Hseu YC, Chen SC, Yech YJ, Wang L, Yang HL. 2008b. Antioxidant activity of Antrodia camphorata on free radical-induced endothelial cell damage. J Ethnopharmacol.
Hsiao G, Shen MY, Lin KH, Lan MH, Wu LY, Chou DS, Lin CH, Su CH, Sheu JR. 2003. Antioxidative and hepatoprotective effects of Antrodia camphorata extract. J Agric Food Chem 51(11):3302-3308.
Huang BM, Hsu CC, Tsai SJ, Sheu CC, Leu SF. 2001a. Effect of Cordyceps sinensis on testosterone priduction in normal mouse Leydig cells. Life Sci 69(22):2593-2602.
Huang BM, Ju SY, Wu CS, Chuang WJ, Sheu CC, Leu SF. 2001b. Cordyceps sinensis and its fractions stimulate MA-10 mouse Leydig tumor cell steroidogenesis. J Androl 22(5):831-837.
Ishikawa NK, Yamaji K, Tahara S, Fukushi Y, Takahashi K. 2000. Highly oxidized cuparene-type sesquiterpenes from a mycelial culture of Flammulina velutipes. Phytochemistry 54(8):777-782.
Kiho T, Tsujimura Y, Sakushima M, Usui S, Ukai S. 1994. [Polysaccharides in fungi. XXXIII. Hypoglycemic activity of an acidic polysaccharide (AC) from Tremella fuciformis]. Yakugaku Zasshi 114(5):308-315.
Kim HG, Shrestha B, Lim SY, Yoon DH, Chang WC, Shin DJ, Han SK, Park SM, Park JH, Park HI, Sung JM, Jang Y, Chung N, Hwang KC, Kim TW. 2006. Cordycepin inhibits lipopolysaccharide-induced inflammation by the suppression of NF-kappaB through Akt and p38 inhibition in RAW 264.7 macrophage cells. Eur J Pharmacol 545(2-3):192-199.
Lee IH, Huang RL, Chen CT, Chen HC, Hsu WC, Lu MK. 2002. Antrodia camphorata polysaccharides exhibit anti-hepatitis B virus effects. FEMS Microbiol Lett 209(1):63-67.
Li SP, Su ZR, Dong TT, Tsim KW. 2002. The fruiting body and its caterpillar host of Cordyceps sinensis show close resemblance in main constituents and anti-oxidation activity. Phytomedicine 9(4):319-324.
Li SP, Zhao KJ, Ji ZN, Song ZH, Dong TT, Lo CK, Cheung JK, Zhu SQ, Tsim KW. 2003. A polysaccharide isolated from Cordyceps sinensis, a traditional Chinese medicine, protects PC12 cells against hydrogen peroxide-induced injury. Life Sci 73(19):2503-2513.
Lu MK, Cheng JJ, Lai WL, Lin YJ, Huang NK. 2008. Fermented Antrodia cinnamomea extract protects rat PC12 cells from serum deprivation-induced apoptosis: the role of the MAPK family. J Agric Food Chem 56(3):865-874.
Lull C, Wichers HJ, Savelkoul HF. 2005. Antiinflammatory and immunomodulating properties of fungal metabolites. Mediators Inflamm 2005(2):63-80.
Mizuno T. 1995. Bioactive biomoleules of mushrooms:Food function and medicinal effect of mushroom fungi. Food Rev.Int.11:7-21
Nakamura K, Yamaguchi Y, Kagota S, Kwon YM, Shinozuka K, Kunitomo M. 1999. Inhibitory effect of Cordyceps sinensis on spontaneous liver metastasis of Lewis lung carcinoma and B16 melanoma cells in syngeneic mice. Jpn J Pharmacol 79(3):335-341.
Oh JY, Baek YM, Kim SW, Hwang HJ, Hwang HS, Lee SH, Yun JW. 2008. Apoptosis of human hepatocarcinoma (HepG2) and neuroblastoma (SKN-SH) cells induced by polysaccharides-peptide complexes produced by submerged mycelial culture of an entomopathogenic fungus Cordyceps sphecocephala. J Microbiol Biotechnol 18(3):512-519.
Ohta Y, Lee JB, Hayashi K, Fujita A, Park DK, Hayashi T. 2007. In vivo anti-influenza virus activity of an immunomodulatory acidic polysaccharide isolated from Cordyceps militaris grown on germinated soybeans. J Agric Food Chem 55(25):10194-10199.
Pan SM, Ye JS, Hseu RS. 1997. Purification and characterization of manganese superoxide dismutase from Ganoderma microsporum. Biochem Mol Biol Int 42(5):1035-1043.
Peng CC, Chen KC, Peng RY, Chyau CC, Su CH, Hsieh-Li HM. 2007. Antrodia camphorata extract induces replicative senescence in superficial TCC, and inhibits the absolute migration capability in invasive bladder carcinoma cells. J Ethnopharmacol 109(1):93-103.
Peng CC, Chen KC, Peng RY, Su CH, Hsieh-Li HM. 2006. Human urinary bladder cancer T24 cells are susceptible to the Antrodia camphorata extracts. Cancer Lett 243(1):109-119.
Rao YK, Fang SH, Tzeng YM. 2007. Evaluation of the anti-inflammatory and anti-proliferation tumoral cells activities of Antrodia camphorata, Cordyceps sinensis, and Cinnamomum osmophloeum bark extracts. J Ethnopharmacol 114(1):78-85.
Shiraga M, Yano S, Yamamoto A, Ogawa H, Goto H, Miki T, Miki K, Zhang H, Sone S. 2002. Organ heterogeneity of host-derived matrix metalloproteinase expression and its involvement in multiple-organ metastasis by lung cancer cell lines. Cancer Res 62(20):5967-5973.
Sirotnak FM, Zakowski MF, Miller VA, Scher HI, Kris MG. 2000. Efficacy of cytotoxic agents against human tumor xenografts is markedly enhanced by coadministration of ZD1839 (Iressa), an inhibitor of EGFR tyrosine kinase. Clin Cancer Res 6(12):4885-4892.
Song TY, Hsu SL, Yen GC. 2005. Induction of apoptosis in human hepatoma cells by mycelia of Antrodia camphorata in submerged culture. J Ethnopharmacol 100(1-2):158-167.
Yang Y, Lv QJ, Du QY, Yang BH, Lin RX, Wang SQ. 2005. Combined effects of Cantide and chemotherapeutic drugs on inhibition of tumor cells' growth in vitro and in vivo. World J Gastroenterol 11(16):2491-2496.
Ye M, Liu JK, Lu ZX, Zhao Y, Liu SF, Li LL, Tan M, Weng XX, Li W, Cao Y. 2005. Grifolin, a potential antitumor natural product from the mushroom Albatrellus confluens, inhibits tumor cell growth by inducing apoptosis in vitro. FEBS Lett 579(16):3437-3443.
Yin AL, Zhong M, Sun GQ, Wang LP, Zhao SS. 2008. [Establishment of a nude mouse model bearing orthotopically transplanted human ovarian carcinoma expressing green fluorescent protein]. Nan Fang Yi Ke Da Xue Xue Bao 28(3):484-486.
Yoo HS, Shin JW, Cho JH, Son CG, Lee YW, Park SY, Cho CK. 2004. Effects of Cordyceps militaris extract on angiogenesis and tumor growth. Acta Pharmacol Sin 25(5):657-665.
Yoshikawa N, Nakamura K, Yamaguchi Y, Kagota S, Shinozuka K, Kunitomo M. 2007. Reinforcement of antitumor effect of Cordyceps sinensis by 2'-deoxycoformycin, an adenosine deaminase inhibitor. In Vivo 21(2):291-295.
Zhao T, Xia WH, Zheng MQ, Lu CQ, Han X, Sun YJ. 2008. Surgical excision promotes tumor growth and metastasis by promoting expression of MMP-9 and VEGF in a breast cancer model. Exp Oncol 30(1):60-64.
Zhu JS, Halpern GM, Jones K. 1998a. The scientific rediscovery of a precious ancient Chinese herbal regimen: Cordyceps sinensis: part II. J Altern Complement Med 4(4):429-457.
Zhu JS, Halpern GM, Jones K. 1998b. The scientific rediscovery of an ancient Chinese herbal medicine: Cordyceps sinensis: part I. J Altern Complement Med 4(3):289-303.
連結至畢業學校之論文網頁點我開啟連結
註: 此連結為研究生畢業學校所提供,不一定有電子全文可供下載,若連結有誤,請點選上方之〝勘誤回報〞功能,我們會盡快修正,謝謝!
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔