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研究生:曾暐婷
研究生(外文):Tseng, Wei-Ting
論文名稱:牛樟芝Antrodia camphorata NPU-44菌絲體固態發酵多醣體結構特徵與其免疫調節之效應
論文名稱(外文):Structural characterizations and immunomodulatory effects of polysaccharides produced by Antrodia camphorata NPU-44 mycelia obtained from solid-state fermentation
指導教授:郭嘉信郭嘉信引用關係
指導教授(外文):Guo, Jia-Hsin
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:食品科學系所
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:88
中文關鍵詞:牛樟芝菌絲體固態發酵多醣體免疫調節結構特徵
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本論文探討牛樟芝Antrodia camphorata NPU-44菌絲體固態發酵多醣體結構特徵與其免疫調節之效應,本研究內容正申請專利中,暫不公開。
摘要……………………………………………………………………… I
Abstract…………………………………………………………………… III
謝誌……………………………………………………………………… V
目錄……………………………………………………………………… VI
圖表目錄…………………………………………………………………. X
壹、 前言……………………………………………………………… 1
貳、 文獻回顧…………………………………………………………. 4
一、樟芝簡介…………………………………………………… 4
(一) 牛樟芝子實體與菌絲體型態…………………………. 4
(二) 牛樟芝命名史………………………………………… 4
(三) 牛樟芝菌種鑑定…………………………………........ 6
二、牛樟芝機能性成分及其生物活性…………………………. 6
(一) 固醇類及其生物活性………………………………… 6
(二) 三萜類及其生物活性…………………………………. 7
(三)核苷類及其生物活性…………………………………... 7
(四)多醣體及其生物活性…………………………………... 8
(五)其他生物活性…………………………………………... 8
1. 抗氧化活性………………………………………….. 8
2. 護肝生物活性……………………………………….. 8
三、多醣體之功能性機制………………………………………. 9
(ㄧ) 多醣體免疫活性機制…………………………………. 9
(二) 多醣體結構與其免疫活性之關連……………………. 10
四、牛樟芝菌絲體多醣體結構特徵…………………………….. 14
五、多醣體之結構分析原理…………………………………….. 14
(ㄧ) 利用高效能液相層析儀測定分子量之原理與應用………………………………………………………
14
(二) 利用氣相層析儀測定單醣組成之原理與應用……… 15
(三) 利用氣相層析質譜儀測定醣苷鍵結之原理與應用… 15
(四) 利用傅立葉紅外線光譜測定官能基團之原理與應用………………………………………………………
16
(五)利用核磁共振光譜儀鑑定結構之原理與應用……… 16
參、 材料與方法…………………………………………………......... 22
一、實驗設計…………………………………………………….. 22
二、材料與方法………………………………………………….. 23
(一) 材料……………………………………………………... 23
(二) 分離菌株………………………………………………... 23
1. 平板培養基配製…………………………………….. 23
2. 液態培養基配製…………………………………….. 23
3. 菌種分離…………………………………………….. 24
(三)樟芝固態發酵……………………………………………. 24
1. 胚芽米固態培養基配製…………………………….. 24
2. 接種菌株…………………………………………….. 24
(四)菌株鑑定…………………………………………………. 25
1. 萃取純化DNA………………………………………. 25
2. 聚合酶連鎖反應…………………………………….. 25
3. 電泳膠分析………………………………………… 25
(五)萃取樟芝固態發酵物之粗多醣體…………………....... 26
(六)去鹽…………………………………………………....... 26
(七)多醣體濃度的測定…………………………………....... 27
1. 標準品……………………………………………… 27
2. 粗多醣樣品………………………………………… 27
(八)分離純化粗多醣體……………………………………... 27
(九)分子量測定………………………………………..……. 27
1. 標準曲線…………………………………………… 27
2. 樣品分析…………………………………………… 28
(十)單糖組成分析………………………………………....... 28
(十一)多醣體鍵結分析……………………………...……… 29
(十二)官能基分析………………………………………....... 29
(十三)結構鑑定…………………………………………....... 30
(十四)免疫力測試………………………………………....... 30
1. 實驗動物…………………………………………… 30
2. 採集老鼠血液及脾臟……………………………… 30
3. 周邊血液單核細胞分離…………………………… 30
4. 脾臟細胞分離……………………………………… 30
5. 總免疫細胞增殖作用……………………………… 31
(十五) 製備抗ACP-179之多株抗體……………………… 31
1. 實驗動物…………………………………………… 31
2. 免疫兔子…………………………………………… 31
3. 間接性酵素連結免疫吸附分析法………………… 32
4. 免疫擴散法………………………………………… 32
肆、 結果與討論………………………………………………………. 34
ㄧ、菌種鑑定…………………………………………………… 34
二、牛樟芝菌絲體固態發酵及其粗多醣體製備……………… 34
三、分離及純化牛樟芝菌絲體固態發酵物之粗多醣體……… 41
(一) 分離及純化粗多醣體…………………………………. 41
(二) 多醣體分子量…………………………………………. 45
四、總免疫細胞增殖…………………………………………….. 51
五、多醣體的單糖組成………………………………………….. 56
六、多醣體鍵結………………………………………………….. 56
七、官能基分析………………………………………………….. 62
八、結構鑑定……………………………………………………. 67
九、間接性酵素連結免疫吸附及免疫擴散測試………………. 71
伍、 結論…………………………………………………………......... 80
參考文獻…………………………………………………………………. 81
作者簡介…………………………………………………………………. 88
沈立言、陳怡欣、林文川。2001。牛樟芝菌絲體發酵萃取液對肝臟生理機能之影響。中華民國食品科學技術學會第三十一次會員大會論文摘要。
林永皓 (2004) 樟芝之牛樟樹宿主專一性。食品工業 36(5):57-70。
林志遠。2005。牛樟芝子實體形成之探討。國立東華大學生物技術研究所碩士論文。
康名慰。2002。香菇菌種之胞外水溶性多醣體分析。國立交通大學生物科技研究所碩士論文。
張惟杰。1999。糖複合物生化研究技術 (第二版)。浙江大學出版社。
陳志昇。1999。冬蟲夏草 Cordyceps sinensis (Berk.) Sacc. 分子生物鑑定系統之研究。國立台灣大學農業化學研究所碩士論文。
陳啟楨、蘇慶華、藍明煌 (2001) 樟芝固態栽培及其生物活性之研究。Fungi Science 16(1,2): 65-72。
黃事暖、蔣昌鐘、劉葉高、吳用堅、林新暖 (2000) 袋栽香菇 “惊菇” 誘導出菇技術。中國食用菌。19(2):24。
劉吉成、牛英才。2008。多醣藥物學 (第一版)。人民生出版社。
戴宇昀。2001。樟芝菌絲體與子實體對四氯化碳及酒精誘導之慢性及急性肝損傷之保肝功能評估。國立中興大學食品科學研究所碩士論文。
Abel G, Czop JK. 1992. Stimulation of human monocyte beta-glucan receptors by glucan particles induces production of TNF-alpha and IL-1 beta. International Journal of Immunopharmacology 14:1363-73.
Ao ZH, Xu ZH, Lu ZM, Xu HY, Zhang XM, Dou WF. 2009. Niuchangchih (Antrodia camphorata) and its potential in treating liver diseases. Journal of Ethnopharmacology 121:194-212.
Bao XF, Duan JY, Fang XY, Fang JN. 2001b. Chemical modifications of the (1→3)-α-D-glucan from spores of Ganoderma lucidum and investigation of their physicochemical properties and immunological activity. Carbohydrate Research 336:127-40.
Bao XF, Liu CP, Fang JN, Li XY. 2001a. Structural and immunological studies of a major polysaccharide from spores of Ganoderma lucidum
(Fr.) Karst. Carbohydrate Research 332:67-74.
Battle J, Ha TZ, Li CF, Della B, Rice P, Kalbfleisch J. 1998. Ligand binding to the (1→3)-β-D-glucan receptor stimulates NFκB activation, but not apoptosis in U937 cells. Biochemical and Biophysical Research Communications 249:499-504.
Bleicher P, Mackin W. 1995. Betafectin P.G.G.-glucan: a novel carbohydrate immunomodulator with anti-infective properties. Annual Review of Pharmacology and Toxicology 37-143-66.
Bock BK, Pedersen C, Pedersen H. 1984. Resonance data for oligosaccharides. Advances in Carbohydrate Chemistry and Biochemistry 42:193-25.
Chang CY, Lue MY, Pan TM. 2005. Determination of Adenosine, Cordycepin and Ergosterol contents in cultivated Antrodia camphorate by HPLC method. Journal of Food and Drug Analysis 13:338-42.
Chang TT, Chou WN. 1995. Antrodia cinnamomea sp. nov. on Cinnamomea kanenirai in Taiwan. Mycology Research 99:756-8.
Chen CC, Liu YW, Ker YB, Wu YY, Lai EY, Chyau CC, Hseu TH, Peng RY. 2007. Chemical characterization and anti-inflammatory effect of polysaccharides fractionated from submerge-cultured Antrodia camphorata Mycelia. Journal of Agricultural and Food Chemistry 55:5007-12.
Chen YJ, Cheng PC, Lin CN, Liao HF, Chen YY, Chen CC, Lee KM. 2008. Polysaccharides from Antrodia camphorata mycelia extracts possess immunomodulatory activity and inhibits infection of Schistosoma mansoni. International Immunopharmacology 8:458-67.
Cheng JJ, Huang NK, Chang TT, Wang DL, Lu MK. 2005. Study for anti-angiogenic activities of polysaccharides isolated from Antrodia cinnamomea in endothelial cells. Life Sciences 76:3029-42.
Cheng JJ, Huang NK, Lur HS, Kuo CI, Lu MK. 2009. Characterization and biological functions of sulfated polysaccharides from sulfated-salt treatment of Antrodia cinnamomea. Process Biochemistry 1-7.
Cheng JJ, Yang CJ, Cheng CH, Wang YT, Huang NK, Lu MK. 2005b. Characterization and functional study of Antrodia camphorata lipopolysaccharide. Joural of Agricultural and Food Chemistry 53:469-74.
Cherng IH, Wu DP, Chiahg HC. 1995. Triterpenoids from Antrodia cinnamomea. Phytochemistry 41:263-7.
Cho SM, Park JS, Kim KP, Cha DY, Yoo ID. 1999. Chemical features and purification of immunostimulation polysaccharides from the fruit bodies of Agaricus blazei. Korea Journal of Mycology 27:170-4.
Dams E. 1988. Campilation of small ribosomal subunit RNA sequences. Nucleic Acids Research 16:87-173.
Doita M, Rasmussen LT, Seljelid R, Lipsky PE. 1991. Effect of soluble aminated beta-1,3-D-polyglucose on human monocytes: stimulation of cytokine and prostaglandin E2 production but not antigen-presenting function. Journal of Leukocyte Biology 49:342-51.
Duncan CJ, Pugh N, Pasco DS, Ross SA. 2002. Isolation of a galactomannan that enhances macrophage activation from the edible fungus Morchella esculenta. Journal of Agricultural and Food Chemistry 50:5683-5.
Habijani J, Berovi M, Wraber B, Hodzar D, Boh B. 2001. Immunostimulatory effects of fungal polysaccharides from Ganoderma lucidum submerged biomass cultivation. Food Technology Biotechnology 39:327-31.
Han HF, Nakamura N, Zuo F, Hirakawa A, Yokozawa T, Hattori M. 2006. Protective effects of a neutral polysaccharide isolated from the mycelium of Antrodia cinnamomea on Propionibacterium acnes and lipopolysaccharide induced hepatic injury in mice. Chemical and Pharmaceutical Bulletin 54(4):496-500.
Hibbett DS. 2006. A phylogenetic overview of the Agaricomycotina. Mycologia 98(6):917-25.
Ho YC, Lin MT, Duan KJ, Chen YS. 2008. The hepatoprotective activity against ethanol-induced cytotoxicity by aqueous extract of Antrodia cinnamomea. Journal of the Chinese Institute of Chemical Engineers 39:441-7.
Hseu YC, Chang WC, Hseu YT, Lee CY, Yech YJ, Chen PC, Chen JY, Yang HL. 2002. Protection of oxidative damage by aqueous extract from Antrodia camphorata mycelia in normal human erythrocytes. Life sciences 71:469-82.
Igor AS, Mark TQ. 2006. Botanical polysaccharides: Macrophage immunomodulation and therapeutic potential. International Immunopharmacology 6:317-33.
Kuo MC, Chang CY, Cheng TL, Wu MJ. 2008. Immunomodulatory effect of Antrodia camphorata mycelia and culture filtrate. Journal of Ethnopharmacology 120:196-203.
Lee IH, Huang RL, Chen CT, Chen HC, Hsu WC, Lu MK. 2002. Antrodia camphorata polysaccharides exhibit anti-hepatitis B virus effects. FEMS Microbiology Letters 209:63-7.
Leung MYK, Liu C, Koon JCM, Fung KP. 2006. Polysaccharide biological response modifiers. Immunology Letters 105:101-14.
Lin JY, Wu TZ, Chou JC. 2005. In vitro induction of fruiting body in Antrodia cinnamomea – a medicinally important fungus. Botanical Studies 47:267-72.
Liu JJ, Huang TS, Hsu ML, Chen CC, Lin WS, Lu FJ, Chang WH. 2004. Antitumor effects of the partially purified polysaccharides from Antrodia camphorata and the mechanism of its action. Toxicology and Applied Pharmacology 201:186-93.
Liu JJ, Huang TS, Hsu ML, Chen CC, Lin WS. 2004. Antitumor effects of the partially purified polysaccharides from Antrodia camphorata and the mechanism of its action. Toxicology and Applied Pharmacology 201:186-93.
Lu MC, Hwang SL, Chang FR, Chen YH, Chang TT, Hung CS, Wang CL, Chu YH, Pan SH, Wu YC. 2009. Immunostimulatory effect of Antrodia camphorata extract on functional maturation of dendritic cells. Food Chemistry 113:1049-57.
Lu ZM, Tao WY, Zou XL, Fu HZ, Ao ZH. 2007. Protective effects of mycelia of Antrodia camphorata and Armillariella tabescens in submerged culture against ethanol-induced hepatic toxicity in rats. Journal of Ethnopharmacology 110:160-4.
Machova E, Kogan G, Alfoldi J, Soltes L, Sandula J. 1995. Enzymatic and ultrasonic depolymerization of carboxymethylated β-1,3-D-glucans derived from Saccharomyces cerevisiae. Journal of Applied Polymer Science 55: 699-704.
Mau JL, Huang PN, Huang SJ, Chen CC. 2003. Time course for antioxidants production by Antrodia camphorata in submerged culture. Fungi Science 18(3,4):59-71.
Mizuno T. 1999. The extraction and development of antitumor-active polysaccharides from medicinal mushrooms in Japan [Review]. International Journal of Medicinal Mushrooms 1:9-30.
Moradali MF, Mostafavi H, Ghods S, Hedjaroude GA. 2007. Immunomodulating and anticancer agents in the realm of macromycetes fungi (macrofungi). International Immunopharmacology 7:701-24.
Morgan CL, Newman DJ, Price CP. 1996. Immunosensors: Technology and opportunities in laboratory medicine. Clinical Chemistry 42(2):193-209.
Poutsiaka DD, Mengozzi M, Vannier E, Sinha B, Dinarello CA. 1993. Cross-linkiing of the beta-glucan receptor on human monocytes results in interleukin-1 receptor antagonist but interleukin-1 production. Blood 82:3695-700.
Ramussen LT, Seljelid R. 1991. Noval immunomodulators with pronounced in vivo effects caused by stimulation of cytokine relase. Journal of Cellular Biochemistry 46:60-8.
Schepetkin IA, Quinn MT. 2006. Botanical polysaccharides: Macrophage immunomodulation and therapeutic potential. International Immunopharmacology 6:317-33.
Shen YC, Chou CJ, Wang YH, Chen CF, Chou YC, Lu MK. 2004. Anti-inflammatory activity of the extracts from mycelia of Antrodia camphorata cultured with water-soluble fractions from five different Cinnamooun species. FEMS Microbiology Letters 231:137-43.
Song TY, Yen GC. 2002. Antioxidant properties of Antrodia camphorata in submerged culture. Journal of Agricultural and Food Chemistry 50: 3322-7.
Song TY, Yen GC. 2003. Protective effects of fermented filtrate from Antrodia camphorate in submerged culture against CCl4-induced hepatic toxicity in rats. Journal of Agricultural and Food Chemistry 51:1571-7.
Tsai MC, Song TY, Shih PH, Yen GC. 2007. Antioxidant properties of water-soluble polysaccharides from Antrodia cinnamomea in submerged culture. 2007. Food Chemistry 104:1115-22.
Wang Q, Fang Y. 2004. Analysis of sugars in traditional Chinese drugs. Journal of Chromatography B 812:309-24.
White TJ, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genesfor phylogenetics In: PCR protocols. A Guide to Methods and Applications 315-22.
Woese CR, Olsen GJ. 1986. Archaebacteria phylogeny: perspectives on the urkingdoms. Systematic and Applied Microbiology 7:161-77.
Wu CY, Liang ZC. 2005. Antimicrobial activity of extract from Antrodia camphorata cultured on pearl barley by solid-state fermentation. Taiwanese Journal of Agricultural Chemistry and Food Science 43(4):295-303.
Wu SH, Yu ZH, Dai YC, Chen CT, Su CH, Chen LC, Hsu WC, Hwang GY. 2004. Taiwanofungus, a polypore new genus. Fungi Science 19(3,4):109-16.
Wu YY, Chen CC, Chyau CC, Chung SY, Liu YW. 2007c. Modulation of inflammation-related genes of polysaccharides fractionated from mycelia of medicinal basidiomycete Antrodia camphorata. Acta Pharmacologica Sinica 28:258-67.
Yamada H, Komiyama K, Kiyohara H, Cyong JC, Hirakawa Y, Otsuka Y. 1990. Structural characterization and antitumor activity of a Pectic polysaccharide from the roots of Angelica acutiloba. Planta Medica 56:182-6.
Yang SW, Shen YC, Chen CH. 1995. Steroids and triterpenoids of Antrodia cinnamomea-fungus parasitic on Cinnamomum micranthum. Phytochemostry 41:1389-92.
Ye LB, Zhang JS, Ye XJ, Tang QJ, Liu YF, Gong CY, Du XJ, Pan YJ. 2008. Structural elucidation of the polysaccharide moiety of a glycopeptide (GLPCW-II) from Ganoderma lucidum fruiting bodies. Carbohydrate Research 343:746-52.
Yu R, Yin Y, Yang W, Ma W, Yang L, Chen X, Zhang Z. 2008. Structural elucidation and biological activity of a novel polysaccharide by alkaline extraction from cultured Cordyceps militaris. Carbohydrate Polymers 1-6.
Zhou X, Lin J, Yin Y, Zhao J, Sun X, Tang K. 2007. Ganodermataceae: Natural products and their related pharmacological functions. The American Journal of Chinese Medicine 35(4):559-74.
Zhuang C, Mizuno T, Ito H, Shimura K, Sumiya T. 1994. Chemical modification and antitumor activity of polysaccharides from the mycelium of liquid-cultured Grifola frondosa. Nippon Shokuhin Kogyo Gakkaishi 41:733-40.
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