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研究生:李育純
研究生(外文):Yu-Chun Lee
論文名稱:牛樟芝保護大鼠肝臟避免酒精餵食引發傷害之研究
論文名稱(外文):Protective effect of Antrodia camphorata on liver from injury of alcohol feeding in rat
指導教授:馮琮涵馮琮涵引用關係
學位類別:碩士
校院名稱:臺北醫學大學
系所名稱:醫學科學研究所
學門:醫藥衛生學門
學類:醫學學類
論文出版年:2009
畢業學年度:97
語文別:中文
論文頁數:44
中文關鍵詞:牛樟芝肝臟酒精
外文關鍵詞:Antrodia camphorataliveralcohol
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許多研究顯示,慢性飲酒會導致血液中的adiponectin蛋白質降低,進而影響肝細胞的脂質代謝,導致脂肪肝的形成與肝臟的纖維化病變。關於急性飲酒是否對肝臟與血中adiponectin濃度造成影響,則尚未有研究報導。另一方面,研究報導傳統中藥樟芝具有保護肝臟、抗氧化與抗發炎等功能;對於急性飲酒,樟芝是否具有快速有效降低血中酒精濃度等解酒的作用,對血液中adiponectin濃度又是否有影響,也是值得探討的問題。
因此本研究以大鼠為材料,實驗分成三組,樟芝(A)組(1.0 g/kg),酒精(E)組(5.5 g/kg),以及樟芝與酒精的混合液(A+E)組。分別在餵食後不同時間(0分鐘、30分鐘、60分鐘、120分鐘、240分鐘與360分鐘)進行採血,利用酵素免疫分析試劑(ELISA kits)測量血中酒精以及adiponectin濃度。結果顯示:A組血中酒精濃度在不同時間點與尚未餵食的0分鐘比較,皆沒有顯著變化。E組血中酒精濃度會逐漸上升,在120分鐘左右達到最高值(185.6 ± 31.4 mg/dL),而後逐漸下降,在360分鐘後血中酒精濃度與0分鐘的基準值比較沒有統計差異。另外,A+E組對血中酒精濃度上升的趨勢與E組並沒有明顯不同。所以結果顯示,餵食樟芝無法有效降低急性酒精餵食後上升的血中酒精濃度。
另一方面,E組在120分鐘內並不會改變血液中adiponectin的濃度;但是在240分鐘與360分鐘之後,血液中adiponectin的濃度分別約為0分鐘的0.94倍與0.95倍,有些微下降的現象,但是不具統計意義。A組對血液中adiponectin濃度沒有顯著影響。A+E組血液中adiponectin濃度在不同時間點也都沒有上升的情形。
綜合以上結果顯示,急性飲酒後,服用樟芝並不影響血液中酒精與adiponectin的濃度。因此樟芝的解酒與保肝作用,推測可能是透過保護肝臟細胞膜上的adiponectin receptor 2,避免酒精的傷害。目前正積極分析急性飲酒以及餵食樟芝後是否影響肝臟細胞的adiponectin receptor 2含量。期待能更進一步了解樟芝保護肝細胞的機轉。
Several studies found that chronic ethanol intake decreased circulating adiponectin level, disrupted lipid metabolism in hepatocytes, and caused excessive lipid accumulation with the development of fatty liver and hepatic steatosis. However, the effects of acute ethanol feeding on circulating adiponectin level and liver have not been examined. Besides, Antrodia camphorate, a traditional Chinese medicine, was found to exert hepatoprotective, anti-oxidative and anti-inflammation effects. In this study, we examined the role of Antrodia camphorata in circulating ethanol and adponectin levels upon acute ethanol feeding in rats. Rats were divided into three groups: one group was Antrodia camphorata (1.0 g/kg) feeding, another group was ethanol (5.5g/kg) feeding, and the other group was fed with the mixture of Antrodia camphorata and alcohol. Blood sample was collected and assayed by ELISA kits at indicated time points. The results showed that Antrodia camphorata feeding did not affect the circulating ethanol levels. After acute alcohol intake, the serum ethanol levels were significantly increased to the maximum value at 120 minutes (185.6 ± 31.4 mg/dL) and then decreased to the basal level (94.2 ± 23.6 mg/dL) at 360 minutes in ethanol-fed rats. Furthermore, the circulating ethanol levels in mixture-fed rats were similar to those in ethanol-fed rats at each indicated time points. Therefore, Antrodia camphorata administration did not attenuate acute ethanol feeding-induced elevation of serum ethanol levels.
On the other hand, there was no effect of alcohol feeding on serum adiponectin levels during 120 minutes. Adiponectin levels were increased to 1.16-fold and 1.15-fold at 240 minutes and 360 minutes, respectively. However, there was no significant difference compared with 0 minute group. Given the mixture of Antrodia camphorata and alcohol or Antrodia camphorata alone did not significantly affect adiponectin serum level.
These results suggested that Antrodia camphorata intake did not suppress serum ethanol or adiponectin levels in ethanol-fed rats. We proposed that the hepatoprotective effect of Antrodia camphorata might be due to protect the adiponectin receptor 2 (Adipo R2) on cell membrane of hepatocyte from ethanol injury. It is important to examine the Adipo R2 content in ethanol-fed and in Antrodia camphorate-fed groups. Furthermore, the mechanism of hepatoprotective effect of Antrodia camphorate could be explored.
目錄......................................................I
中文摘要................................................III
英文摘要..................................................V
縮寫表..................................................VII
第一章 緒論..............................................1
第一節 Adiponectin簡介...............................1
第二節 酒精簡介......................................4
第三節 樟芝(Antrodia camphorata)簡介.................6
第四節 研究目的......................................7
第二章 實驗材料..........................................9
第一節 實驗動物......................................9
第二節 樟芝..........................................9
第三節 試藥..........................................9
第四節 儀器.........................................10
第五節 實驗設計.....................................10
第六節 流程圖.......................................11
第七節 分組方式.....................................11
第三章 實驗方法.........................................12
第一節 急性餵食.....................................12
第二節 慢性餵食.....................................17
第三節 統計分析.....................................18
第四章 實驗結果.........................................19
第一節 不同方式給予酒精血中Ethanol濃度變化..........19
第二節 口服餵食樟芝及酒精血中Ethanol濃度變化........19
第三節 長期餵食酒精後血中Adiponectin濃度變化........20
第四節 口服餵食樟芝及酒精血中Adiponectin濃度變化....20
第五節 長期餵食酒精後血中三酸甘油酯濃度變化.........21
第六節 長期餵食酒精後肝臟三酸甘油酯濃度變化.........21
第五章 討論.............................................23
第六章 結論.............................................26
參考文獻.................................................27
實驗圖表.................................................33
附錄圖表.................................................39
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