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研究生:劉宗鑫
研究生(外文):Tsung Hsin Liu
論文名稱:多孔矽在光學生物感測之研究
論文名稱(外文):The Study of Porous Silicon Used in Pptical Biosensing
指導教授:蕭桂森
指導教授(外文):Vincent K.S. Hsiao
學位類別:碩士
校院名稱:國立暨南國際大學
系所名稱:生物醫學科技研究所
學門:醫藥衛生學門
學類:醫學技術及檢驗學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
論文頁數:49
中文關鍵詞:多孔矽
外文關鍵詞:Porous Silicon
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多孔矽(Porous Silicon)是以半導體材料─單晶矽(Cystalline silicon)為基材,經由電化學蝕刻(Electrochemistry Etching)的方式,施予電壓後和氫氟酸(HF)產生電化學反應,並且在矽的表面蝕刻出具有週期性排列的微小孔狀的結構,之後在光學偵測上可透過反射(reflection)的方式進行光傳導與偵測。
在本論文中,我們以自製組裝的蝕刻設備,將鐵氟龍、O-ring(防溢橡膠墊圈)以及黃金線電極的結合成為耐酸設備。單晶矽經由電化學蝕刻後,表面會形成週期性排列的結構的孔洞,之後再利用掃描式電子顯微鏡(Scanning Electron Microscope SEM)去觀察不同條件下的多孔矽結構變化,之後透過白光反射傳導去偵測多孔矽的光學訊號,同時,再透過微流道元件(Micro-flow-channel)製作技術去結合多孔矽,形成不受外部因素影響的微流道式的多孔矽,然後預期做出能偵測去氧核醣核酸(DeoxyriboNucleicAcid DNA)的生物感測元件,在本實驗中,我們已經製造出能夠偵測出濃度為100nM的去氧核醣核酸,之後再嘗試利用即時監測方式去偵測不同濃度的去氧核糖核酸。
The porous silicon are structures which base by semiconducting material—crystalline silicon, by electrochemical etching and given voltage and hydrofluoric acid (HF) on the substrate to cause electrochemical reaction. Moreover, etch periodically arranged microstructures holes on the surface of the silicon, then we can proceed waveguide and detection by reflection on optical detection. In this thesis ,Used self-assembled etching equipment to combine with O-rings, Electrodes ,Teflon tubes and plates to get acid-resisting equipment. Crystalline silicon form cycled porous film after electrochemistry etching. Then can observe the change of the structure of porous silicon under different conditions using scanning electron microscope (SEM), and detect the optical signal of porous silicon by the reflection conduction of white light. At the same time, combine the technology of micro-flow-channel to porous silicon to form porous silicon that would not affect by external factors. Then expect to make a biosensor that could detect deoxyribonucleic acid (DNA).
中文摘要........................................................................................................................I
英文摘要........................................................................................................................II
圖目錄........................................................................................................................IV
表目錄.........................................................................................................................V
第一章 簡介................................................................................................................1
1.1 前言.............................................................................................1
1.2 研究動機與目的............................................................................2
第二章 原理................................................................................................................4
2.1多孔矽的形成機制.........................................................................4
2.1.1多孔矽的理論模型...........................................................................8
2.2多孔矽之光學性質........................................................................10
2.2.1單層多孔矽光學行為.....................................................................11
2.3 去氧核醣核酸基本理論與架構......................................................12
2.3.1去氧核醣核酸(deoxyribonucleic acid,DNA).........................12
2.3.2表面官能活化.................................................................................14
2.4生物分子固定術............................................................................15
第三章 實驗材料與方法..........................................................................................17
3.1藥品.............................................................................................17
3.2儀器設備......................................................................................17
3.3微流道多孔矽實驗流程.................................................................18
3.4多孔矽的製備...............................................................................19
3.5微流道的製作...............................................................................21
3.6光學系統架設方法........................................................................23
3.6.1反射光譜系統架設.........................................................................23
3.6.2光致發光系統架設.........................................................................24
3.7多孔矽的表面改質........................................................................25
3.7.1利用APTES修飾多孔矽...................................................................25
3.7.2去氧核醣核酸雜合反應實驗流程.................................................27
第四章結果與討論....................................................................................................30
4.1多孔矽的光激發光(Photo Luminescence,PL)量測..........................30
4.2 不同蝕刻條件來觀察多孔矽..........................................................31
4.3 多孔矽的表面改質.......................................................................36
4.3.1多孔矽修飾上APTES後的穩定度...................................................37
4.4多孔矽結合微流道........................................................................38
4.4.1觀察微流道多孔矽在水中的穩定度.............................................39
4.5 微流道式的多孔矽感測DNA雜合反應..............................................41
4.5.1不同濃度的DNA之雜合反應...........................................................43
第五章 結論與未來展望..........................................................................................45
第六章參考文獻........................................................................................................46
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