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研究生:林宗玄
研究生(外文):Tsung-Hsuan Lin
論文名稱:用毛細管電泳分析補充牛樟芝萃取物後的抗氧化能力
論文名稱(外文):Analysis of Anti-Oxidant ability supplements Antrodia Camphorate extracts by Capillary electrophoresis
指導教授:王海龍王海龍引用關係黃維寧
指導教授(外文):Hai-Lung WangWei-Ning Huang
學位類別:碩士
校院名稱:元培科技大學
系所名稱:生物技術研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
論文頁數:58
中文關鍵詞:衰竭運動毛細管電泳氧化壓力脂質過氧化反應
外文關鍵詞:exhaustive exerciseCapillary electrophoresisOxidative Stresslipid peroxide
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本研究主要在探討補充牛樟芝萃取物後,對於高血脂老鼠從事衰竭運動後,體內的生理變化。以80隻雄性 Sprague Dawley 老鼠為實驗對象,隨機分配為血脂組、低、中、高劑量組。低劑量組補充量為0.1 g / kg / day 、中劑量組為0.3 g / kg / day 、高劑量組為1 g / kg / day 。牛樟芝萃取物連續補充四星期之後,再將老鼠細分為衰竭運動組與非衰竭運動組,每組各10隻。從事衰竭運動的老鼠在老鼠專用跑步機上以坡度10%,每分鐘15公尺的速度先跑10分鐘熱身,然後逐漸增加速度與時間,直到跑至衰竭為止。之後犧牲,採集血液樣品及鼠腦做分析。比較補充牛樟芝萃取物與衰竭運動二個因子,對於老鼠體內血液生化分析值、血脂狀態以及氧化壓力的變化。實驗結果顯示,補充牛樟芝萃取物對於衰竭運動後體內 GPT, TG, LDH, CPK, 四項生化分析值是有顯著的差異性(p <0.05)但大腦脂質過氧化物 MDA 的測定及毛細管電泳分析GSH, GSSG 後的結果,則無明顯變化。
This study focuses on the rats with hyperlipidemia and their body’s physiological changes after exercise and intake of Antrodia Camphorate extract supplement. The 80 male Sprague Dawley rats are the experiment subjects; they were randomly assigned in the low, medium, and high-dose groups. A dose of 0.1 g / kg / day was administered for the low-dose group, 0.3 g /kg /day for the medium-dose group, and 1g / kg / day for the high-dose group. After administering the Antrodia Camphorate extract for 4 consecutive weeks, the rats were then sub-divided into the exhaustive exercise group and non-exhaustive exercise group that each consists of 10 rats. The rats of the exhaustive exercise group were first placed on a rodent treadmill of 10% slope where they warmed up for 10 minutes at 15 meters/minute, until the rats run up to failure. Afterwards, the rats were sacrificed to collect blood samples and remove the brain for analysis. The two factors of the Antrodia Camphorate extract supplement and exhaust exercise were then compared to determine the biochemical analysis values in the bloodstream of the rats, the blood lipid status, and oxidative stress changes. The experimental results have indicated that after intake of the Antrodia Camphorate extract supplement, the four biochemical analysis values of GPT, TG, LDG, and CPK found in the body have reached significant differences (p &lt;0.05), but the results for the lipid peroxide MDA in the brain and the capillary electrophoresis analysis results for GSH and GSSG have not shown remarkable changes.
誌謝 I
中文摘要 II
英文摘要 III
目錄 IV
圖目錄 VIII
表目錄 X
第一章 緒論1
1.1血脂異常的定義2
1.2自由基參與脂質過氧化反應的化學機轉與動脈硬2
1.3樟芝3
1.3 Glutathione 4
第二章 毛細管電泳的介紹5
2.1電泳 5
2.2毛細管電泳演進5
2.3毛細管電泳的原理6
2.3.1電滲透流6
2.3.2理論板數7
2.4毛細管電泳進樣方式7
2.4.1電動注入法( electrokinetic injection )7
2.4.2重力注入法( gravity injection )7
2.4.3壓力注入法( pressure injection )8
2.5 界面活性劑8
2.6 毛細管電泳的分離模式8
2.6.1 Capillary Zone Electrophoresis (CZE)8
2.6.2 Micellar Electro Kinetic Capillary Chromatography( MEKC )8
2.6.3 Capillary Gel Electrophoresis (CGE)9
2.6.4 Capillary Isoelectric Focusing (CIEF)9
2.7 毛細管電泳的優點9
第三章 研究目的10
第四章 材料與方法11
4.1試驗動物11
4.1.1各分組再細分為運動組與非運動組11
4.1.2儀器設備11
4.1.3實驗步驟11
4.1.4血液分析12
4.1.5血液分析方法原理12
4.1.6統計分析13
4.2 脂質過氧化物丙二醛(Malondialdehyde; MDA)之分析14
4.2.1 器官均質液之製備14
4.2.2 α-Thiobarbituric acid 衍生物之備14
4.2.3 MDA 標準液之製備14
4.2.4 器官均質液中 MDA 衍生物及 MDA 標準液濃度之測定驟14
4.2.5 統計分析15
4.3 毛細管電泳儀設備15
4.3.1 毛細管電泳系統15
4.3.2 其他設備15
4.3.3 分析試藥15
4.3.4實驗方法16
4.3.5 毛細管初次使用活化流程16
4.3.6實驗流程17
4.3.7 檢量線製作17
第五章 結果與討論18
5.1牛樟芝萃取物對於老鼠衰竭運動後血液丙氨酸氨基氨酶( GPT )的變化18
5.2牛樟芝萃取物對於老鼠衰竭運動後血液總膽固醇( TC )的變化18
5.3牛樟芝萃取物對於老鼠衰竭運動後血液三酸甘油脂( TG )的變化18
5.4牛樟芝萃取物對於老鼠衰竭運動後血液高密度脂蛋白膽固醇( HDL-C )的變化19
5.5牛樟芝萃取物對於老鼠衰竭運動後血液低密度脂蛋白膽固醇( LDL-C )的變化19
5.6牛樟芝萃取物對於老鼠衰竭運動後血液乳酸脫氫酶( LDH )的變化20
5.7 牛樟芝萃取物對於老鼠衰竭運動後血液肌酸激酶( CK )的變化20
5.8 牛樟芝萃取物對於老鼠衰竭運動後大腦組織丙二醛( MDA )的變化21
5.9 中、高劑量組與中、高劑量運動組血漿中 GSH, GSSG 濃度變化21
第六章結論23
參考文獻24
圖表28



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二、中文部分
1.鍾子雯、陳福財、劉珍芳:接受耐力訓練之運動選手其飲食狀況與體內抗氧化力之評估。中華營誌 24:339-350 (1999)
2.謝錦城、許壬榮、呂學冠、廖威彰、劉燦榮(1998):衰竭運動對老鼠紅血球脂質過氧化物、變形性與形態學的影響。中華民國大專院校體育總會八十七年度體育學術研討會專刊,中華民國大專院校體育總會出版,9,17-28。
3.臨床生物化學( Clinical Chemistry ),第四版,何敏夫 編著 2005年10月 合記圖書出版社發行
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