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研究生:渡部容子
研究生(外文):Watanabe yoko
論文名稱:樟芝對thioacetamide誘導大鼠肝纖維化的抑制效果
論文名稱(外文):The Inhibitory effect of Antrodia cinnamomea onthioacetamide induced hepatic fibrosis in rat
指導教授:吳金濱吳金濱引用關係
學位類別:碩士
校院名稱:中國醫藥大學
系所名稱:藥物化學研究所碩士班
學門:醫藥衛生學門
學類:藥學學類
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:71
中文關鍵詞:肝纖維化硫代乙醯胺樟芝
外文關鍵詞:hepatic fibrosisthioacetamideAntrodia cinnamomea
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樟芝為台灣特有的藥用植物主要應用於肝臟疾病。在本實驗,探討樟芝對硫代乙醯胺(thioacetamide;TAA)誘導大鼠肝臟纖維化的效果,及其作用機轉。每組大鼠12隻,分為控制組、TAA+CMC組、TAA+樟芝(131、393 mg/kg) 組等。給予樟芝的大鼠能降低血中AST跟ALT活性。樟芝能減少肝臟hydroxyproline及malondiladehyde含量。樟芝能增加肝臟抗氧化物質glutathione的含量。病理組織的檢查,樟芝能改善結節增生及膽管纖維化。RT-PCR的分析顯示,樟芝能抑制肝臟損傷的指標的MAT2A的mRNA的表現。庫氏細胞的活性指標CD14的mRNA表現也會被抑制。肝臟纖維化指標的collagen (I) ??1 及tissue inhibitor of metallopeptidase 1 mRNA的表現也被抑制。西方墨點法分析顯示,樟芝可以抑制星狀細胞活化的指標??-smooth muscle actin的表現。樟芝可以減少肝臟中γ-glutamyltranspeptidase的活性,同時也可以抑制在Glutathione S-transferase (GST)-M、GST-Placental form
的基因表現。這些基因表現被認為與前癌肝臟(preneoplastic liver)有關係。
結論,本研究確認樟芝能延緩TAA誘導大鼠肝臟纖維及前癌肝臟的產生。樟芝有可能用於預防肝臟纖維及肝癌。


Antrodia cinnamomea (AC) is a kind of mushroom only grows in Taiwan. AC has been used as a drug for liver protection and the treatment of the folk medicine. In this study, the effect of AC to thioacetamide (TAA) induced rat liver fibrosis was investigated. Rat was separated in to 4 groups, each group includes 12 animals, which were control group, TAA + CMC group, TAA + AC (131, 393 mg/kg) group. Rats treated with AC showed lower AST, ALT than the group which didn`t treated with AC. The increased the amount of hepatic hydroxyproline and malondiladehyde was inhibited. These results showed an increase in antioxidant of glutathione. Pathological examination showed that AC improved nodular hyperplasia, fibrosis. RT-PCR analysis showed the AC inhibited the expression of mRNA of MAT2A which is an indicator of liver damage. The indicator of Kupffer cell activity, mRNA of CD14 was inhibited. The indicator of liver fibrosis, the mRNA expression of collagen I (??1) and tissue inhibitor of metallopeptidase 1 weres inhibited. Western Blotting analysis showed that, AC inhibited ??-smooth muscle actin expression. In addition, AC can reduce the amount of γ-glutamyltranspeptidase in the liver. The results of RT-PCR analysis, Glutathione S-transferase (GST)-M、GST-Placental form expression were reduced. These are believed to suppress preneoplastic liver.
In conclusion, the present study has demonstrated that AC retards the progression of liver fibrosis and neoplastic liver in TAA-treated rats. It may be expected that AC has preventive potential in liver fibrosis and hepatoma.


目録 .................................................................................................. I
縮寫對照表 ...................................................................................... III
圖目錄 ............................................................................................... V
表目錄 .............................................................................................. VI
摘要(中文)..................................................................................... VIII
摘要(英文)..................................................................................... IX
第一章 緒論 ....................................................................................... 1
一、 研究背景與目的 ............................................................ 1
二、 肝纖維化形成之機制 .................................................... 3
(一) 肝臟纖維化之成因................................................... 3
(二) 肝臟構造................................................................... 4
(三) 硫代乙醯胺誘導之肝纖維化及肝臟前癌模式 ..... 8
三、 牛樟芝之文獻探討 ...................................................... 11
(一) 牛樟芝形態 ........................................................... 11
(二) 牛樟芝化學成分研究 ........................................... 13
(三) 牛樟芝藥理研究 ................................................... 13
第二章 材料與方法 ......................................................................... 18
第三章 結果 ..................................................................................... 32
第四章 討論 ..................................................................................... 58
結論 .................................................................................................. 63
參考文獻 .......................................................................................... 64


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