跳到主要內容

臺灣博碩士論文加值系統

(18.97.14.82) 您好!臺灣時間:2025/02/19 10:24
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:李杰鴻
研究生(外文):Li Chieh-Hung
論文名稱:以北蕉與南華蕉果實作為樟芝培養基質之複合碳源研究
論文名稱(外文):Uaing ‘Pei-Jaou’ and ‘Naiwa’ Banana Species as Carbon Compound for Antrodia Cinnamomea Cultivating Media
指導教授:李柏旻
指導教授(外文):Li Pomin
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:生物機電工程系所
學門:工程學門
學類:機械工程學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:70
中文關鍵詞:牛樟芝北蕉南華蕉脂肪酶胞外多醣
外文關鍵詞:Antrodia cinnamomeaPei-JaouNaiwalipaseextracellular polysaccharide
相關次數:
  • 被引用被引用:1
  • 點閱點閱:837
  • 評分評分:
  • 下載下載:15
  • 收藏至我的研究室書目清單書目收藏:1
樟芝(Antrodia cinnamomea)為台灣特有的藥用菇類,但由於樟芝生長緩慢且取之不易,因此近年來大多使用人工培養,本研究探討以不同品種香蕉果實作為樟芝培養基質之複合碳源,探討碳源改變對樟芝多醣體合成與脂肪酶活性之影響。試驗結果顯示,香蕉果實作為培養基碳源是可行的;北蕉未催熟碳源全取代有最佳之多醣體產量及脂肪酶活性,其多醣體為3.5770 gmL-1及脂肪酶活性5.613 UmL-1,可利用此特性將香蕉果實作為替代培養基碳源,替代傳統化學培養基,做為香蕉產銷失衡時之對應方案。
Antrodia cinnamomea is a kind of precious medicinal mushrooms in Taiwan, But due Antrodia cinnamomea very slow grownd, so in recent years, mostly using artificial cultivationto, this study explores the matrix of the composite carbon as Antrodia cinnamomea cultured with different varieties of banana fruits ources to explore the carbon source to change the effect of Antrodia cinnamomea polysaccharides synthesis and lipase activity. The results show that the banana fruit as a medium carbon source is feasible; North banana ripening is not carbon source to replace the whole polysaccharide production and lipase activity, polysaccharides 3.5770 gmL-1 and lipase activity of 5.613 UmL-1, this feature can be used banana fruit as an alternative medium carbon source, an alternative to traditional chemical medium, as the imbalance in banana production and marketing of the corresponding program.
摘要
Abstract:
誌謝
目錄
第一章 緒論
1.1 研究背景
1.2 研究背景
1.3 研究目的
第二章 文獻回顧
2.1 食藥用菇類簡介
2.2 食藥用菇類的生理活性
2.3 食用藥菇液態培養之特性
2.4 人工培養基的組成
2.4.1 碳源
2.4.2氮源
2.4.3 碳氮比
2.4.4 無機鹽類
2.5 人工培養環境因子
2.5.1 搖晃速率與剪切力
2.5.2 溫度
2.5.3 pH值
2.6 食用藥菇液態培養之特性
2.7香蕉
2.8樟芝相關研究
2.9脂肪酶相關研究
2.10醣類與脂質在微生物學及生物化學基礎理論
第三章 材料與方法
3.1 供試材料
3.1.1 樟芝來源
3. 1.2 樟芝培養基複合碳源之來源
3.2 處理方法
3.2.1 樟芝繼代與保存之固態培養基(MEA)
3.2.2 樟芝菌絲體之保存
3.2.3 樟芝菌絲體活化與繼代培養
3. 2.4 香蕉果實預處理
3.2.5 酵母抽取物液態培養基(Yeast Extract, YM)與香蕉果實複合碳源培養基
3.2.6 樟芝液態培養醱酵液處理
3.3 檢測項目與方法
3.3.1 樟芝培養液之多醣萃取與分析
3.3.2 樟芝醱酵液之脂肪酶活性測定
3.4 研究數據統計分析 SAS統計分析軟體分析
3.5 實驗流程圖
3.6 試驗藥品與儀器
3.6.1 實驗藥品
表3-4. 實驗藥品成分與廠牌
3.6. 2 實驗儀器
表3-5. 實驗儀器設備之廠商與規格
第4章 結果與討論
4.1結果探討
4.1.1北蕉及南華蕉果實作為樟芝培養基對合成多醣體及脂肪酶活性影響
4.1.2北蕉及南華蕉果實催熟及未催熟作為樟芝培養基對合成多醣體及脂肪酶活性影響
4.1.3 北蕉及南華蕉果實催熟及未催熟調整添加比例作為樟芝培養基對合成多醣體及脂肪酶活性影響
4.1.4 添加不同品種香蕉複合碳源培養基對樟芝菌絲體之影響
第5章 結論
參考文獻
附錄

1. Abel H, Marie D, Nathalie R, Danielle D, Louis S, Louis C. (2000). Purification and characterization of an extracellular lipase from a thermophilic Rhizopus oryzae strain isolated from palm fruit. Enzyme Microb Technol. 26, 421-30.
2. Brand-Williams, W., M.E. Cuvelier, &; C. Berset. (1995). Use of a free radical method to evaluate antio-xidantactivity. Lebensmittel -Wissenschaft und-Technologie. 28, 25-30.
3. Chang, T.T., &; W.R. Wang. (2005).Basidiomatal formation of Antrodia cinnamomea on artificial agar media. Bot. Bull. Acad. Sin. 46, 151-154.
4. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F.(1956).Colorimetric method for determination of sugars and related substances.Anal. Chem. 28, 350-356.
5. Eo, S.K., Y.S. Kim, C.K. Lee, &; S.S. Han. (2000). Possible mode of antiviral activity of acidic protein bound polysaccharide isolated from Ganoderma lucidum on herpes simplex viruses. J. Ethnopharmacol. 72(3), 475-481.
6. Hirasawa, M., N. Shouji, T. Neta, K. Fukushima, &; K. Takada. (1999). Three kinds of antibacterial substances from Lentinus edodes (Berk.) Sing. (Shiitake , an edible mushroom). Int J Antimicrob Agents. 11, 151-157.
7. Jong, S.C., &; J.M. Birmingham. (1993). Medicinal and therapeutic value of the Shiitake mushroom. Adv. Appl. Microbiol. 39, 153-184.
8. J.P. Essien, E.J. Akpan, &; E.P. (2005). Essien Studies on mould growth and biomass production using waste banana peel. Bioresource Technology, 96, 1451-1456.
9. Kim, Y.S., S.K. Eo, K.W. Oh, C.K. Lee, &; S.S. Han. (2000). Antiherpetic activities of acidic protein bound polysacchride isolated from Ganoderma lucidum alone and in combinations with interferons . J. Ethnopharmacol. 72(3), 451-458.
10. Kendrick, A., &; C. Ratledge. (1996). Cessation of polyunsaturated fatty acid formation in four selected filamentous fungi when grown on plant oils. J Am Oil Chem Soc. 73, 431-435.
11. Kojima I., H. Yoshikawa, M. Okazaki, &; Z. Terui. (1972). Studies on riboflavin pro¬duction by Eremothecium ashbyii. J. Ferment Technol. 50, 716-723.
12. Lee, B.C., J.T. Bae, H.B. Pyo, T.B. Choe, S.W. Kim, H.J. Hwang, &; J.W. Yun. (2003). Biological activities of the polysaccharides produced from submerged culture of the edible Basidiomycete Grifola frondosa. Enzyme and Microbial Technology . 32, 574-581.
13. Lee D, Koh Y, Kim K, Kim B, Choi H, Kim D, et al. (1999). Isolation and characterisation of thermophilic lipase from Bacillus thermoleovorans ID- 1. FEMS Microbiol Lett .179, 393-400.
14. Lee, I.H., C.T. Chen, H.C. Chen, W.C. Hsu &; M.K. Lu.(2002). Sugar flux in response to carbohydrate-feeding of cultured Antrodia camphorata, a recently described medicinal fungus in Taiwan. Journal of Chinese Medicine. 13(1), 21-31.
15. Lee, I.H., Huang, R.L., Chen, C.T., Chen, H.C., Hsu, W.C., Lu, M.K., (2002). Antrodia camphorata polysaccharides exhibit antihepatitis B virus effects. FEMS Microbiol. Lett. 209, 63-67.
16. Lee, S. S., Y.H. Wei, C.F Chen., S.Y. Wang, &; K.Y. Chen. (1995). Antitumor effects of Ganoderma lucidum. J. Chin. Med. 6(1), 1-12.
17. Lingnert, H., K. Vallentin, &; C.E. Eriksson. (1979). Measurement of antioxidative effect in model system, Journal of Food Protection and Preservation. 3, 87-103.
18. Mao, X.B., T. Eksriwong, S. Chauvatcharin, &; J.J. Zhong.(2005). Optimization of carbon source and carbon/nitrogen ratio for cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris. Process Biochemistry. 40, 1667-1672.
19. Morita, K., &; S. Kobayashi. (1967). Isolation, structure, and synthesis of lenthionine and its analogs. Chem Pharm Bull. 15, 988-993.
20. Razak A, Samel MYA. Basri M, Yunus WMZW. Ampon K, Salleh AB. (1994). Thermostable extracellular protease of Bacillus stearothermophilus— factors affecting its production. World J Microbiol Biotechnol. 10, 260–3.
21. Singh. (2010). Harnessing the potential of banana and plantain in Asia and Pacific for inclusive growth. In “ISHS/ProMusa Symposium on Global Perspectives on Asia Challenges” Guangzhou, China. 14-18th, Sept, 2009.
22. Sinchaikul S, Sookkheo B, Phutrakul S, Pan FM, Chen ST. (2001). Optimization of a thermostable lipase from Bacillus stearothermophilus P1: overexpression, purification, and characterization. Protein Expr Purif. 22, 388-98.
23. Song, C.H., Jeon, Y.J., Yang, B.K., Ra, K.S., Sung, J.M. (1998). The anticomplementary activity of exo-polymers produced from submerged mycelial cultures of higher fungi with particular reference to Cordyceps militaris. J. Microbiol. Biotechnol. 8, 536-539.
24. Stasinopoulos, S.J., &; R.J. Seviour. (1990). Stimulation of exopolysaccharide production in the fungus Acremonium persicinum with fatty acids. Biotech Bioeng. 36, 778-782.
25. Suzuk,i H., A. Okubo,S. Yamazaki, K. Suzuki, H. Mitsuya, &; S. Toda. (1989). Inhibition of the infectivity and cytopathic effect of human immunodeciency virus by water-soluble lignin in an extract of the culture medium of Lentinus edodes mycelia (LEM). Biochem Biophys Res. Commun. 160, 367-373.
26. Takazawa, H., F. Tajima, &; C. Miyashita. (1982). An antifungal compound from Shiitake (Lentinus edodes).Yakugaku Zasshi. 102, 489-491.
27. Tang, Y. J., &; J. J. Zhong.(2002). Fed-batch fermentation of Ganoderma lucidum for hyperproduction of polysaccharide and ganoderic acid. Enzyme microb. Technol. 31, 20-28.
28. Tochikura, T.S., H. Nakashima, &; N. Yamamoto. (1989). Antiviral agents with activity against human retroviruses. J. Acquir. Immune. Def Syndr. 2, 441-447.
29. Tsai, Z. T. &; Liaw, S. L. (1985). The use and the effect of Ganoderma. San Yun Press: Taichung, Taiwan, pp.116.
30. Vorderwiilbecke T, Kieslich K, Erdmann H. (1992). Comparison of lipases by different assays. Enzyme Microb Technol. 14, 631-9.
31. Wang, S.Y., M.L. Hsu., H.C. Hsu, C.H. Tzeng, S.S. Lee, M.S. Shiao, &; C.K. Ho. (1997). The anti-tumor effect of Ganoderma lucidum is mediated by cytokines released from activated macrophages and T lymphocytes. Int. J . Cancer. 70, 699-705.
32. Wang, Y. Y., Khoo, K. H., Chen, S. T., Lin, C. C., Wong, C. H., &; Lin, C. H.(2002). Studies on the immuno-modulating and antitumor activities of Ganoderma lucidum (Reishi) polysaccharides: functional and proteomic analyses of a fucose-containing glycoprotein fraction responsible for the activities. Bioorganic &; Medicinal Chemistry. 10, 1057-1062.
33. Winkler UK, Stuckmann M. (1979). Glycogen hyaluronate and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens. J Bacteriol 138, 663-70.
34. Wu, J.Z., C.K.C. Peter, K.H. Wong, &; N.L. Huang. (2004). Studies on submerged fermentation of Pleurotus tuber-regium (Fr.) Singer. Part 2: effect of carbon-to-nitrogen ratio of the culture medium on the content and composition of the mycelial dietary fibre , Food Chemistry 85(1), 101-105.
35. Xu, C.P., S.W. Kim, H.J. Hwang &; J.W. Choi.(2003). Optimization of submerged culture conditions for mycelial growth and exo-
biopolymer production by Paecilomyces tenuipes C240. Process Biochemistry. 38, 1025-1030
36. Yasumoto, K., K. Iwami, &; H. Mitsuda. (1971). A new sulphur-containing peptide from Lentinus edodes acting as precursor for lenthionine. Agric. Bio.l Chem.35 , 2059-2069.
37. 行政院農業委員會(2010)。農業統計年報,香蕉。
38. 王伯徹(1992)。認識食藥用菇。食品工業,24(12):8-15。
39. 水野卓、川合正允,賴慶亮譯(1997)。菇類的化學。生化學,國立編譯館,台北,255頁。
40. 李一宏(2003)。樟芝菌絲體之培養及其多醣體抗乙型肝炎病毒活性評估。中國醫藥學院中國藥學研究所,29:15-19頁。
41. 何仁為(2005)。深層培養環境對生產豬苓(Polyporus umbellatus)菌絲體及多醣體之影響。東海大學食品科學系碩士論文,132頁。
42. 林順富(2007)。生物化學。偉明圖書有限公司,台北。
43. 林樹錢(2000)。菌絲生長條件,中國藥用菌生產與產品開發,P335-337。
44. 高曉薇(1991)。台灣靈芝新種靈芝之三帖類成分研究。臺北醫學院天然藥物醫學研究所碩士。
45. 張志堂(2003)。培養條件對靈芝多醣生成及性質之影響。東海大學化學工程學系碩士論文,215頁。
46. 張東柱(1997)。牛樟之病害。牛樟生物學及育林技術研討會論文集,林業叢刊第72號,12(3):127-131頁。
47. 張淑芬(2001)。食藥用菇類搖瓶液體培養條件之探討。食品工業 33(7):41-42。
48. 黃玉如(2005)。液態培養條件對巴西蘑菇(Agaricus blazei Murill)菌絲體及胞外多醣體生成之影響。國立屏東科技大學農園生產系碩士論文,88頁。
49. 黃鈴娟(2000)。樟芝與姬松茸之抗氧化性質及其多醣組成分析。國立中興大學食品科學系碩士論文,148頁。
50. 黃薺緯(2003)。金頂側耳之呈味特性和抗氧化及抗致突變性質。國立中興大學食品科學系碩士論文,173頁。
51. 陳清農、高炳煌、楊梅春、陳勁初(1999)。台灣森林中的寶石樟芝。鄉間小路,1:22-23頁。
52. 陳清農、許勝傑、陳勁初(2000)。紅色旋風勢不可擋再談台灣樟芝。鄉間小路,26(5):48-50頁。
53. 範貞綺(2003)。樟芝固態栽培與液態發酵菌絲體之成份及其活性之研究。南台科技大學化學工程研究所。
54. 劉佳茹(2005)。油脂與界面活性劑對舞菇生長與多醣生產之影響。大葉大學生物產業科技學系碩士論文,131頁。
55. 蔡雁暉(2002)。樟芝深層培養液及其多醣體之抗氧化特性。國立中興大學食品科學研究所碩士論文。
56. 蔣雅慧(2004)。添加物對樟芝菌絲與抗氧化活性生成之影響。東海大學化學工程學系碩士論文,62頁。

連結至畢業學校之論文網頁點我開啟連結
註: 此連結為研究生畢業學校所提供,不一定有電子全文可供下載,若連結有誤,請點選上方之〝勘誤回報〞功能,我們會盡快修正,謝謝!
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top