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研究生:劉家如
研究生(外文):Chia-Ju Liu
論文名稱:酚類化合物對聚木糖酶助漂闊葉樹硫酸鹽漿之影響
論文名稱(外文):Effect of phenolic compounds on xylanase assisted bleaching for hardwood kraft pulp
指導教授:柯淳涵柯淳涵引用關係
指導教授(外文):Chun-Han Ko
口試委員:張上鎮蕭英倫
口試日期:2009-06-18
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:森林環境暨資源學研究所
學門:農業科學學門
學類:林業學類
論文種類:學術論文
論文出版年:2009
畢業學年度:100
語文別:英文
論文頁數:75
中文關鍵詞:漂白選擇性鄰苯二酚癒創木酚卡巴值香草醛黏度聚木糖酶
外文關鍵詞:bleaching selectivitycatecholguaiacolkappa numbervanillinviscosityxylanase
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紙廠在製漿造紙過程中產生的黑液,含大量木質素及相關降解物質,其中最常見的物質為酚類化合物,因此紙漿漂白前都要先用大量的水清洗紙漿,以去除附著在紙漿上的黑液,方可進行後續的漂白流程。許多研究指出聚木糖酶對於紙漿漂白有助漂的效果。本研究係模擬紙廠的紙漿進行漂白效能的探討,即酚類化合物存在於紙漿時對於紙漿漂白效能的影響,及上述現象對聚木糖酶協助紙漿漂白效果的影響,目的在於瞭解聚木糖酶的助漂是否可節省漂白前洗漿過程中水資源的使用。
本研究首先探討酚類化合物對一種商用聚木糖酶(Pulpzyme HC)活性的影響。接著取氧漂前及氧漂後的紙漿進行漂白,於酚類化合物存在時進行聚木糖酶助漂,配合二氧化氯漂白及鹼萃,測定經上述處理的紙漿之卡巴值及黏度變化,以瞭解酚類化合物對漂白效果之影響。
研究結果顯示,6種酚類化合物與聚木糖酶反應時,僅有鄰苯二酚及香草醛會影響此商用聚木糖酶的活性,提高酚類化合物濃度,酵素活性降低,另外4種酚類化合物則對此商用聚木糖酶的活性沒有顯著的影響。3種酚類化合物(鄰苯二酚、香草酸、癒創木酚)的存在,會降低漂白的效果,卡巴值及黏度皆隨著酚類化合物濃度的提高而下降愈少。將此商用聚木糖酶應用於含鄰苯二酚的紙漿中進行助漂,卡巴值及黏度均較只加入此商用聚木糖酶助漂的紙漿來得高,顯示鄰苯二酚會降低此商用聚木糖酶的助漂效果。
漂白選擇性(Bleaching selectivity)係漂白效果的表現,研究結果顯示酚類化合物濃度較高時會降低漂白選擇性,而添加此商用聚木糖酶則是提高漂白選擇性;酚類化合物存在時,以此商用聚木糖酶處理紙漿的漂白選擇性則介於上述二者中間,顯示出聚木糖酶對於紙漿漂白的助漂效果,可以抵消酚類化合物對於紙漿漂白效果造成的負面影響。


Black liquor produced from kraft pulp contained lot of lignin and lignin-degrading products similar to the structure of phenolic compounds. The most common compounds in black liquor were phenolic compounds. In pulp and paper mill, it would consume lots of water to remove the black liquor then continued to pulp bleaching sequences. Many studies showed that adding xylanase into pulp bleaching sequences could improve the bleaching efficiency. This study simulated the bleaching efficiency in pulp and paper mill to investigate the impact of phenolic compounds on pretreatment of pulp and the impact of phenolic compounds on xylanase pretreatment of pulp. The purpose of this study mainly discussed that the prebleaching ability of xylanase whether reduce the use of water in the process of pulp washing before bleaching.
The study first investigated the impact of phenolic compounds on the activities of Pulpzyme HC. Unbleached and oxygen bleached pulp were used in this study. Adding xylanase into pulp with phenolic compounds subjected to ClO2 bleaching and alkaline extraction. To understand the impact of phenolic compounds on bleaching efficiency measured kappa number and viscosity of the pulp
The six phenolic compounds reacted with xylanase, Pulpzyme HC, only catechol and vanillin acid reduced the xylanase activities. The higher concentration of phenolic compounds, the lower the xylanase activities were. The other phenolic compounds showed no significant effect on xylanase activities. The bleaching efficiency of pulp with phenolic compounds (catechol, vanillin acid and guaiacol) was reduced. Kappa number and viscosity reduced less when adding higher concentrations of phenolic compounds. Adding xylanase into prebleaching sequence of pulp with phenolic compounds, kappa number and viscosity were higher than only adding xylanase. It showed that catechol lowered the prebleaching efficiency of Pulpzyme HC.
Bleaching selectivity could represent the bleaching efficiency. The presence of phenolic compounds was shoed to reduce bleaching selectivity, but Pulpzyme HC prebleaching would increase the bleaching selectivity. The bleaching selectivity of xylanase into pulp prebleaching with phenolic compounds was between solely phenolic compounds and solely Pulpzyme HC. It showed that the bleaching efficiency of adding xylanase into prebleaching sequences could neutralize negative impact of phenolic compounds on prebleaching of pulp.


摘要 i
Abstract ii
Index iv
Table index vi
Figure Index vii
I. Introduction 1
II. Literature review 3
2.1 Xylanase 3
2.2 Pulping liquors 5
2.2.1 Kraft black liquors 5
2.2.2 Lignin and its degradation products 5
2.2.3 Degradation products of lignin 7
2.3 Pulp screening, cleaning and washing 9
2.3.1 The drum displacer 9
2.3.2 Brownstock washing experience 10
2.4 Bleaching sequences 13
2.4.1 Chlorination 13
2.4.2 Chlorine dioxide bleaching 13
2.4.3 Alkaline extraction 15
2.5 Xylanase treatment with phenolic compounds 15
III. Materials and methods 18
3.1. Research framework 18
3.2. Raw materials 19
3.3 Xylanase activity assay 20
3.4. Impact of phenolic compounds on xylanase activity 21
3.5. Prebleaching of Kraft pulps 21
3.5.1 Prebleaching with phenolic compounds pretreatment before ClO2 bleaching 22
3.5.2 Prebleaching with xylanase and phenolic compounds pretreatment before ClO2 bleaching 22
3.5.3 Alkaline extraction 22
3.6. Kappa number 22
3.7. Viscosity 23
3.8. Bleaching selectivity 23
IV. Results and discussion 24
4.1. Impact of phenolic compounds on the activities of Pulpzyme HC 24
4.2. Impact of phenolic compounds on prebleaching of pulp 32
4.3. Impact of phenolic compounds on xylanase prebleaching of pulp 42
4.4. Impacts of adding phenolic compound and xylanase into pulp prebleaching on bleaching selectivity 63
V. Conclusions 68
VI. References 70


Alen, R. and Vikkula, A., "Formation of lignin monomers during alkaline delignification of softwood", Holzforschung, 43, pp. 397-400(1989).
Alen, R. and Vikkula, A., "Formation of lignin monomers during kraft pulping of birch wood", Cellulose Chemistry and Technology, 23, pp. 579-583(1989).
Bajpai, P., Bhardwaj, N.K., Bajpai, P.K., and Jauhari, M.B., "The impact of xylanases on bleaching of eucalyptus kraft pulp", Journal of Biotechnology, 38, pp. 1-6(1994).
Beg, Q. K., Kapoor, M., Mahajan, L., and Hoondal, G.S., "Microbial xylanases and their industrial applications: a review", Applied Microbiology and Biotechnology, 56, pp. 326-338(2001).
Deshpande, M.V. and Eriksson, K.E., "Reutilization of enzymes for saccharification of lignocellulosic materials", Enzyme and Microbial Technology, 6, pp. 338-340(1984).
Garg, A.P., Roberts, J.C., and McCarthy, A.J., "Bleach boosting effect of cellulase-free xylanase of Streptomyces thermoviolaceus and its comparison with two commercial enzyme preparations on birchwood kraft pulp", Enzyme and Microbial Technology, 22, pp. 594-598(1998).

Gierer, J. and Lindeberg, O., "Reactions of lignin during sulfate pulping. Isolation and identification of new dimers from a spent sulfate liquor", Acta Chemica Scandinavica, Series B: Organic Chemistry and Biochemistry, 34, pp. 161-170(1980).
Gierer, J. and Wannstrom, S., "Formation of alkali-stable C-C-bonds between lignin and carbohydrate fragments during kraft pulping", Holzforschung, 38, pp. 181-184(1984).
Johan, G. and Carl-Johan, F (Eds.)., "Fiber line operation". Chemical Pulping. Helsinki, Finland. pp. A158-A189(2000).
Johan, G. and Carl-Johan, F (Eds.)., "Pulping washing application", Chemical Pulping. Helsinki, Finland, pp. A582-A588(2000).
Kaya, F., Heitmann Jr, J.A., and Joyce, T.W., "Cellulase binding to cellulose fibers in high shear fields", Journal of Biotechnology, 36, pp. 1-10(1994).
Kaya, F., Heitmann, J.A., and Joyce, T.W., "Effect of shear fields on hemicellulase binding to pulp fibers", Journal of Biotechnology, 45, pp. 23-31(1996).
Kaya, F., Heitmann, J.A., and Joyce, T.W., "Influence of lignin and its degradation products on enzymatic hydrolysis of xylan", Journal of Biotechnology, 80, pp. 241-247(2000).
Lowendahl, L., Petersson, G., and Samuelson, O., "Phenolic compounds in kraft black liquor", Svensk Papperstidning, 81, pp. 392-396(1978).
Lappan, R., Pelton, R., McLennan, I., Patry, J., and Hrymak, A., "Kraft lignin poly(DADMAC) precipitate formation", Industrial and Engineering Chemistry Research, 36, pp. 1171-1175(1997).
Morck, R., Reimann A., and Krigstad K.P., "Fractionation of kraft lignin by successive extraction with organic solvents. Ⅲ. Fractionation of kraft lignin from birch", Holzforschung, 42, pp. 111-116(1988).
Morck, R., Yoshida, H., Krigstad K.P., and Hatakeyama, H., "Fractionation of kraft lignin by successive extraction with organic solvents. Ⅰ.Functional groups, 13C-NMR-spectra and molecular weight distributions", Holzforschung, 40, pp. 51-60(1986).
Norrstrom, H. 1970. Spectra of lignins from pulp and spent liquor. Svensk Papperstidning. 73: 619-630.
Oye, R., Langfors, N.G., Phillips, F.H., and Higgins, H.G., "The properties of kraft black liquors from various eucalypts and mixed Tropical Hardwoods", Appita, 31, pp. 33-40(1977).
Paice, M.G., Gurnagul, N., Page, D.H., and Jurasek, L., "Mechanism of hemicellulose-directed prebleaching of kraft pulps", Enzyme and Microbial Technology, 14, pp. 272-276(1992).
Paul, S.S., Kamra, D.N., Sastry, V.R.B., Sahu, N.P., and Kumar, A., "Effect of phenolic monomers on biomass and hydrolytic enzyme activities of an anaerobic fungus isolated from wild nil gai (Baselophus tragocamelus)", Letters in Applied Microbiology, 36, pp. 377-381(2003).
Ragauskas, A.J., Poll, K.M., and Cesternino, A.J., "Effects of xylanase pretreatment procedures on nonchlorine bleaching", Enzyme and Microbial Technology, 16, pp. 492-495(1994).
Senior, D.J. and Hamilton, J., "Use of xylanases to decrease the formation of AOX in kraft pulp bleaching", Journal of Pulp and Paper Science, 18, pp. 165-169(1992).
Senior, D.J., Mayers, P.R., Breuil, C., and Saddler, J.N., "The interaction of xylanase with pulps: non-selective adsorption and inactivation of xylanase. In: Kirk, T.K. and Chang, H.M., (Eds.)", Biotechnology in Pulp and Paper Manufacture. Butterworth–Heinemann, Boston. pp. 169-182(1990).
Sharma, A., Milstein, O., Vered, Y., Gressel, J., and Flowers, H.M., "Effects of aromatic compounds on hemicellulose-degrading enzymes in Aspergillus japonicus", Biotechnology and Bioengineering, 27, pp. 1095-1101(1985).
Niemela K. and Alen, R., "Characterization of pulping liquors", In: Sjostrom, E. amd Raimo, A. (Eds.), Anayltical Methods in Wood Chemistry, Pulping, and Paper making, New York, USA, pp. 205-212(1992).
Sutcliffe, R. and Saddler, J.N., "The role of lignin in the adsorption of cellulases during enzymatic treatment of lignocellulosic material", Biotechnology and Bioengineering Symposium, 17, pp. 749-762(1986).
Tai, D.-S., Chen, C.-L., and Gratzl, J.S., "Chemistry of delignification during kraft pulping of bamboos", Journal of Wood Chemistry and Technology, 10, pp. 75-99(1990).
Thomas, M.G. and Earl, W.M. (Eds.), "Alkaline pulping", Pulp and Paper manufacture, Malcoml, Atlanta, GA, pp. 446(1989).
Tolan, J.S. and Canovas, R.V., "The use of enzymes to decrease the Cl2 requirements in pulp bleaching", Pulp & Paper-Canada, 93, pp. 39-42(1992).
Viikari, L., Kantelinen, A., Sundquist, J., and Linko, M., "Xylanase in bleaching from an idea to the industry", Fems microbiology reviews, 13, pp. 335-350(1994).
Villar, J.C., Caperos, A., and Garcia-Ochoa., "Precipitation of kraft black liquors by alcohol-calcium solutions", Separation Science and Technology, 31, pp. 1721-1739(1996).
Wada, s., Iwamida, T., Iiaima, R., and Yabe, K., "The bonds between thiolignin and synthetic rubber. Ⅶ. fractional precipitation of thiolignin from kraft pulping waste liquor with the variation of pH and reinforcement of SBR with each fraction", Chemistry high polymer (Japan), 19, pp. 699-703(1962).
Wilcox, P.H. and Goring, D.A.J., "The effect of pH on the leaching of lignin from kraft and chemimechanical pulps", Cellulose Chemistry and Technology, 24, pp. 735-740(1990).
Yamasaki, T., Hosoya, S., Chen, C.L., and Gratzl, J.S., "Characterization of residual lignin in kraft pulp", In: Neidhardt, F.C. (Eds.), Proceedings of the International Symposium on Wood and Pulping Chemistry, Stockholm, Swedish, pp. 34-42(1981).


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