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研究生:謝杰儒
研究生(外文):Chieh-Ju Hsieh
論文名稱:牛樟芝萃取物對四氯化碳引發大鼠肝纖維化的保護作用
論文名稱(外文):Protective effect of Taiwanofungus camphoratus extracts on carbon tetrachloride induced liver fibrosis in rats
指導教授:吳金濱吳金濱引用關係
指導教授(外文):Jin-Bin Wu
學位類別:碩士
校院名稱:中國醫藥大學
系所名稱:藥物化學研究所碩士班
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:59
中文關鍵詞:牛樟芝四氯化碳肝臟纖維化
外文關鍵詞:Taiwanofungus camphoratusAntrodia comphorataAntrodia cinnamomealiverfibrosiscarbontetrachloride
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本篇研究是在探討椴木栽培的牛樟芝(Taiwanofungus camphoratus)子實體超臨界萃取物(TCSE)能否改善四氯化碳引起的大鼠肝臟纖維化病症。大鼠每100 g體重經口給予0.2毫升的四氯化碳(20%),每週兩次,持續八週。每天給予TCSE,八週後犧牲大鼠並採取檢體。第八週的alanine transaminase、aspartate transaminase均有顯著下降,白蛋白則是有顯著回升。肝臟檢體的膠原蛋白含量以及切片檢查均顯示TCSE能顯著抑制膠原蛋白的產生。肝臟檢體的Glutathione顯著回升與Malondialdehyde顯著下降顯示TCSE能顯著提升抗氧化能力。免疫組織染色的部份顯示TCSE能有效降低alpha smooth muscle actin (α-SMA)的產生。RT-PCR的數據顯示TCSE能顯著抑制Col1α1與α-SMA兩個基因的表現。
依據以上數據,推論TCSE抑制四氯化碳誘導的肝纖維化,可能是經由抑制肝星狀細胞(hepatic stellate cell;HSC)的活化來達到保肝作用。


This article investigated whether Taiwanofungus camphoratus carpophore''s supercritical extract(TCSE) can improve liver fibrosis on rats induced by carbon tetrachloride. Rats got 0.2 mL carbon tetrachloride (20%) per 100g body weight orally, twice a week, for eight weeks. Rats treated with TCSE, and were sacrificed. The value of aspartatetransaminase and alanine transaminase on the eighth week decreased significantly, and albumin increased. Collagen quantity of liver samples and slides'' check showed that TCSE can inhibit production of collagen. Glutathione increasing significantly and malondialdehyde decreasing significantly showed that TCSE can promote anti-oxidative activity.Tissue immunochromatography slides showed that TCSE can reduce the production of alpha smooth muscle actin (α-SMA).TCSE can also inhibit mRNA of Col1α1 and α-SMA on RT-PCR Data.
According to above data, we can infer that TCSE can inhibit liver fibrosis process induced by carbon tetrachloride and have liver protection, probably through inhibiting activity of hepatic stellate cell(HSC).


目錄.....................................................V
圖表目錄...............................................VII
中文摘要.................................................IX
英文摘要.................................................X
第一章 緒論...............................................1
一、 研究背景與目的..................................1
二、 牛樟芝文獻考察..................................2
(一) 牛樟芝型態......................................3
(二) 牛樟芝段木栽培..................................4
(三) 牛樟芝化學成分..................................5
(四) 牛樟芝藥理研究..................................5
(五) 純化合物的藥理活性..............................8
三、 肝臟纖維化病理機制.............................16
(一) 肝臟纖維化病理成因.............................16
(二) 四氯化碳動物病理模式...........................18
(三) 抗氧化酵素 ....................................20
第二章 材料與方法........................................22
第三章 結果..............................................30
第四章 討論..............................................34
第五章 參考文獻..........................................53


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