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研究生:吳立娟
研究生(外文):Wu, Li-Chuan
論文名稱:對稱型波長編碼共軛焦顯微鏡
論文名稱(外文):Symmetric wavelength-encoded chromatic confocal microscope
指導教授:詹明哲詹明哲引用關係
指導教授(外文):Chen, Ming-Che
學位類別:碩士
校院名稱:國立交通大學
系所名稱:影像與生醫光電研究所
學門:工程學門
學類:生醫工程學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
語文別:中文
論文頁數:57
中文關鍵詞:光學共軛焦顯微鏡光柵稜鏡
外文關鍵詞:confocal microscopygratingprism
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光學共軛焦顯微鏡(Confocal Microscopy,CM)的高解析度可以將活體裡面的構造做虛擬的光學切片,此技術已經成為生物醫學或半導體研究上重要的造影工具。在傳統的光學共軛焦顯微鏡中,需要經由機械式的掃描,來完成二為甚至三維影像,其機械掃瞄的方式不僅增加了整個系統的體積、造價,更降低了成像速度,因此,本研究提出並展示了一種不受機械式掃描的限制之共焦顯微鏡,利用寬頻光源以及共焦顯微系統快速在CCD上產生影像,提供即時的斷層影像,光纖式的架構,取代針孔裝置,增加量測系統的可饒度,並應用於活體生物組織的病理診斷或是工業檢測的細微結構組織等相關技術上。研究結果顯示,利用對稱型波長編碼共軛焦顯微鏡掃描出來的解析度線圖(resolution chart),得到群數是3組數是2,解析度是:8.98 lp/mm 或是 55.68 μm,影像大小約為:0.28mm X 0.14mm。此結果可提供未來二維的成像系統改製為對深度的影像之呈現,亦或利用光柵、稜鏡以及菲涅耳透鏡或梯度折射率透鏡組合成三維的成像系統。
High resolution confocal microscopy is able to perform optical sectioning of the structure in vitro. This technique has been widely applied in the research of biomedical and semiconductor as a significant imaging tool. 2D or 3D confocal microscopy by mechanical scanners not only increases the size and cost of the entire system, but also reduces the imaging speed. To overcome these drawbacks, in this thesis a confocal microscopy system of non-mechanical scan is proposed and demonstrated, which uses broadband source and CCD real time imaging and the pinhole device was replaced by fibers. The resolution of image with a size of 0.28mm X 0.14mm scanned by the symmetric wavelength-encoded chromatic confocal microscope is 8.98 lp/mm or 55.68 μm. This preliminary result provides scanning not only on X-Y plane. Hopefully, 3D imaging systems could be achieved by combining different dispersive elements, such as grating, prism, and Fresnel lens or GRIN lens.
目錄      
中文摘要 ii
英文摘要 iii
致謝 iv
目錄 v
表目錄 viii
圖目錄 ix
ㄧ、緒論 1
1.1 前言 1
1.2 研究動機 4
1.3 論文架構 5
二、實驗理論 7
2.1 顯微鏡的光學理論 7
2.1.1 繞射極限(Diffraction Limit) 10
2.1.2 像差 13
2.2 共軛焦顯微鏡(CM)原理 15
2.3 頻譜編碼共軛焦顯微鏡(SECM)原理 17
2.4 頻譜編碼共軛焦顯微鏡的現行研究狀況 18
2.5 頻譜編碼共軛焦顯微鏡所用到的橫向分佈光學元件 23
2.5.1 光柵(Grating) 23
2.5.2 稜鏡(Prism) 25
2.6 頻譜編碼共軛焦顯微鏡所用到的縱向分佈光學元件 32
2.6.1 菲涅耳透鏡(Fresnel Lens) 32
2.6.2 梯度折射率透鏡(GRIN Lens) 36
2.7 影像的重要參數 37
2.7.1 影像大小 (Image size) 37
2.7.2 解析度 (Resolution) 40
2.7.3 影格速率 (Frame rate) 41
三、實驗架構 42
3.1 實驗裝置 42
3.2 系統特性 45
3.2.1 雷射光源 45
3.2.2 掃描系統 46
3.2.3 偵測訊號處理 46
四、結論與討論 48
4.1 結論 48
4.2 未來展望 52
參考文獻 53
附錄一 56
附錄二 57

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