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研究生:陳韋綸
研究生(外文):Wei-Lun Chen
論文名稱:白牛樟芝與一般牛樟芝的化合物分析
論文名稱(外文):Chemical variation of white Antrodia cinnamomea from regular Antrodia cinnamomea
指導教授:周志青
指導教授(外文):Jyh-Ching Chou
學位類別:碩士
校院名稱:國立東華大學
系所名稱:自然資源與環境學系
學門:環境保護學門
學類:環境資源學類
論文種類:學術論文
論文出版年:2013
畢業學年度:101
論文頁數:102
中文關鍵詞:白牛樟芝三萜類藍光處理
外文關鍵詞:white antrodia cinnamomeatriterpenoidsblue light treatment
相關次數:
  • 被引用被引用:3
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  • 下載下載:411
  • 收藏至我的研究室書目清單書目收藏:5
牛樟芝(Antrodia cinnamomea)和其宿主牛樟樹 (Cinnamomum kanehirae )為台灣本土的特有種。牛樟芝為一種昂貴的藥用真菌,經實驗研究證實牛樟芝富含多醣體和三萜類,對於抗癌、抗發炎、降血壓、抗氧化具有功效。本研究特別針對更為稀有的白牛樟芝,作一系列的研究,藉由白牛樟芝與紅牛樟芝的化合物做分析比較,以及誘導紅牛樟芝白化,進一步探討紅牛樟芝與白牛樟芝的相對關係。本研究利用不同的光照處理,觀察牛樟芝是否因不同刺激產生不同的生長變化。實驗結果,成功以藍光誘導牛樟芝菌絲的白化,同時經由實驗也發現,紅光可以造成牛樟芝不穩定生成子實體的現象。從薄層分析與高效能液相層析圖譜中比較不同的時期的萃取物,可以看出白牛樟芝與紅牛樟芝化合物的差異。而經本實驗誘導白化的牛樟芝在分析圖譜中也較接近白牛樟芝而又保有紅牛樟芝的某些特徵。分析牛樟芝幾個指標性三萜類化合物的改變,得知經誘導白化的牛樟芝,不但三萜類組成接近白牛樟芝,同時也還保留紅牛樟芝的化合物特徵。利用細胞融合技術,本研究進一步探討白牛樟芝是否為基因受損而變異。觀察白色牛樟芝經細胞融合後所挑出菌株的生長狀況,其菌種菌絲呈現紅色,其液態培養基萃取物有別於一般牛樟芝的二次代謝物,但固態培養後期卻又出現白色菌絲的菌落,其詳細機制仍待進一步的分析研究。
Antrodia cinnamomea and its host Cinnamomum kanehirae, are both endemic species unique to Taiwan. A. cinnamomea is an expensive medicinal fungus. Many studies have confirmed that A. cinnamomea is rich in polysaccharides and triterpenoids that have medicinal effects in anti-cancer, anti-inflammation, antihypertensive and anti-oxidation. This study, makes a series of observations of white A. cinnamomea which occurs rarely in nature. Chemical variations among white A. cinnamomea, regular A. cinnamomea and induced white A. cinnamomea were compared. This study, illustrates the relative relationship between red A. cinnamomea and white A. cinnamomea which occur naturally or through radiation induction. Different light radiation was used to stimulate A. cinnamomea. Blue light was found to induce A. cinnamomea mycelia whitening and red light was able to cause irregular formation of A. cinnamomea fruiting bodies. The chemical profiles of A. cinnamomea extracts at different growth stages was compared using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The TLC and HPLC profiles indicate that specific triterpenoids were varied among different A. cinnamomea. The compounds profiles were different between white A. cinnamomea and red A. cinnamomea. Moreover, the compounds of induced white A. cinnamomea were similar to those of naturally occurring white A. cinnamomea but retained specific characteristics of red A. cinnamomea. Another issue explored in this study is whether white A. cinnamomea is genetic modification causes its different growth conditions. Some protoplast fusion strains were made from white A. cinnamomea. Interestingly, the mycelia of the cell-fusion strain was red color. The extraction from liquid culture media showed their secondary metabolites were different between cell-fusion strains and regular A. cinnamomea. Further analysis is needed to verify these variations.
1. 前言 1
1.1 牛樟芝背景及重要性簡介 1
1.2 牛樟芝在分類上的地位 2
1.3 牛樟芝的形態及生長環境 3
1.4 牛樟芝功效及其應用價值 3
1.4.1 抗癌的活性 4
1.4.2 消炎、免疫的調節作用 5
1.4.3 抗B型肝炎 5
1.4.4 抗氧化活性 5
1.4.5 保肝活性 6
1.4.6 肝纖維化的預防 7
1.4.7 降血壓的作用 7
1.5 牛樟芝成分 8
1.5.1 牛樟芝之多醣體 9
1.5.2 牛樟芝之三萜類 9
1.6 其他有效成分 10
1.7 光照在真菌學上的應用 10
2. 研究動機 13
3. 材料方法 15
3.1 牛樟芝菌種記錄與保存 15
3.2 牛樟芝的繼代培養 15
3.3 實驗菌種來源 16
3.4 牛樟芝的光照處理 17
3.5 牛樟芝光照處理後的再生 17
3.6 萃取紅牛樟芝、白牛樟芝、誘導白化牛樟芝之不同時期菌絲 17
3.7 萃取牛樟芝液態培養基之二次代謝物 18
3.8 牛樟芝原生質體的製備 18
3.9 細胞融合 19
3.10 牛樟芝細胞核螢光染色 19
3.11 薄層色層分析 20
3.12 高效能液相層析儀的分析 21
3.13 質譜儀的分析 22
4. 結果 23
4.1 牛樟芝的繼代培養 23
4.2 牛樟芝的光照處理 23
4.3 牛樟芝光照處理後的再生 24
4.4 牛樟芝原生質體的製備 25
4.5 牛樟芝細胞核螢光染色 25
4.6 細胞融合 25
4.7 薄層色層分析 26
4.8 高效能液相層析儀分析 27
4.9 質譜儀分析 29
5. 討論 31
6. 引用文獻 37
7. 圖表 47
8. 附錄 83
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