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研究生:張家源
研究生(外文):Chia-Yuan Chang
論文名稱:牛樟芝培養方式條件對其代謝產物之影響研究
論文名稱(外文):Effects of Photo-fermentation on the Metabolites of Antrodia cinnamonea
指導教授:陳良宇鄭建瑋鄭建瑋引用關係
指導教授(外文):Liang-Yu ChenChien-Wei Cheng
學位類別:碩士
校院名稱:銘傳大學
系所名稱:生物科技學系碩士班
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:中文
論文頁數:80
中文關鍵詞:化學組成分析乙醇萃取抗癌牛樟芝
外文關鍵詞:Antrodia cinnamomeachemical profilinganticancerethanol extract
相關次數:
  • 被引用被引用:5
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  • 下載下載:329
  • 收藏至我的研究室書目清單書目收藏:1
牛樟芝常做為增強免疫力、保肝、抗癌之保健食品素材,是台灣特有且珍貴的藥用真菌,近年來更為醫藥生物資源的研究重點。而本研究主要是要利用光源與培養時間差異,對液態培養牛樟芝之生長進行調控,更探討液態培養牛樟芝代謝物組成變化的影響,以及對癌細胞活性之效能。
化學分析的結果呈現,培養天數越長所得粗三萜檢測之吸光值較高,代表其代謝產物(三萜類及油酸等)的含量越高,而光源強度在15W/m2下培養之最終產物具有較高的生物活性代謝物。
抗癌活性之體外細胞實驗結果表明,液態培養牛樟芝菌絲體之乙醇萃取物對人類子宮頸癌上皮細胞(HeLa)與肝癌細胞(HuH-7)都具有效抑制性與劑量依賴關係,由培養菌絲體之醇抽出物於60μg/ml的劑量下可抑制HeLa 細胞活性達73.6%,與相同劑量下野生牛樟子實體醇抽出物的差異不大。30μg/ml的醇萃取物劑量即可抑制HuH-7 細胞活性達90%,15W/m2培養14天之菌絲體IC50劑量更僅需16μg/ml,相較於野生牛樟子實體42μg/ml是具顯著差異的。因此,本研究證明光發酵技術具有提升牛樟芝功效的應用潛力。
Antrodia cinnamomea is a unique and merit traditional medicinal fungus and used for healthy functions, such as immunity regulation, hepatoprotection, anticancer in Taiwan. The work of this study is to regulate the growth and metabolites of A. cinnamonea in submerged fermentation with color lights. And, the chemical compositions and the cyto-activities of ethanol extracts of fermentation products were evaluated by the GC/MS and the cytotoxicity assay, respectively.
The triterpenoid amounts (A245nm) and chromatograms of submerged mycelium show the complexity of chemical profiling for the increased cultured days under the illumination. The anticancer assay in vitro also indicates that the ethanol extracts have a dosage dependent inhibitory on human cervical epithelioid carcinoma (HeLa) cells, and a well-different way on human hepatoma (HuH-7) cells. The mycelium extract of 60μg/ml inhibits the 73.6% activity of HeLa cell. The 30μg/ml extract also inhibits 90% activity of HuH-7 cell. The IC50 of mycelia extract with the 15W/m2 luminance for 14 days only is 16μg/ml.
The study demonstrates the photo-fermentation has the potential to promote the functional chemicals and bio-activities of A.cinnamonea for economic production.
目錄 I
圖目錄 III
表目錄 IV
附表目錄 V
中文摘要 VI
Abstract VII
第一章研究動機與目的 1
第二章文獻回顧 5
第一節保健食品概述 5
第二節 牛樟芝 5
2.1牛樟芝簡介 5
2.2 牛樟芝之使用歷史與研究趨勢 6
2.3 牛樟芝的商業模式與生產方式 9
2.4 牛樟芝次級代謝物 11
第三章 材料與方法 20
第一節 菌株培養 20
1.1 固態培養 20
1.2 液態培養 20
1.3 液態培養基 20
1.4 固態培養基 21
第二節 儀器 21
2.1 固態與液態培養相關設備 21
2.2 分析與萃取相關設備 21
第三節 藥品 22
3.1 分析用藥品 22
3.2 萃取與分析溶劑 22
3.3 培養基 22
第四節 液態培養方法與萃取條件 22
4.1 搖瓶 22
4.2 LED光照條件設定 23
第五節機能性成分分析方法 25
5.1菌體醇抽出與粗三萜測定 25
5.2氣相層析儀條件設定 25
第六節 生物活性分析方法 26
6.1 細胞培養(Cell culture) 26
6.2細胞存活率分析(MTT assay and MTS assay) 26
第四章 結果與討論 28
第一節牛樟子實體與條件培養菌絲體之粗三萜分析 28
第二節牛樟菌層析比較分析 31
2.1 野生牛樟子實體與牛樟木氣相層析圖 31
2.2 黑暗培養組牛樟菌絲體氣相層析圖 33
2.3 15W/m2培養組牛樟菌絲體氣相層析圖 35
2.4 30W/m2培養組牛樟菌絲體氣相層析圖 37
2.5 40W/m2培養組牛樟菌絲體氣相層析圖 39
第三節 細胞存活率分析 41
3.1樟芝乙醇萃取液對HeLa cell的毒殺效果 41
3.2各培養條件之菌絲體萃取液對HeLa cell的毒殺效果 41
3.3子實體與菌絲體萃取液對HuH-7 cell的毒殺效果 45
3.4醇萃取液對HuH-7 cell型態影響 49
第四章 總結 52
參考文獻 55
附錄 62
[1] Wu SJ, Leu YL, Chen CH, Chao CH, Shen DY, Chan HH, et al. Camphoratins A-J, Potent Cytotoxic and Anti-inflammatory Triterpenoids from the Fruiting Body of Taiwanofungus camphoratus. J Nat Prod. 2010;73:1756-62.
[2] Wang LC, Wang SE, Wang JJ, Tsai TY, Lin CH, Pan TM, et al. In vitro and in vivo comparisons of the effects of the fruiting body and mycelium of Antrodia camphorata against amyloid beta-protein-induced neurotoxicity and memory impairment. Appl Microbiol Biot. 2012;94:1505-19.
[3] Du YC, Chang FR, Wu TY, Hsu YM, El-Shazly M, Chen CF, et al. Antileukemia component, dehydroeburicoic acid from Antrodia camphorata induces DNA damage and apoptosis in vitro and in vivo models. Phytomedicine. 2012;19:788-96.
[4] Chiang CC, Chiang BH. Processing characteristics of submerged fermentation of Antrodia cinnamomea in airlift bioreactor. Biochem Eng J. 2013;73:65-71.
[5] Liu CJ, Chiang CC, Chiang BH. The elicited two-stage submerged cultivation of Antrodia cinnamomea for enhancing triterpenoids production and antitumor activity. Biochem Eng J. 2012;64:48-54.
[6] Liu DZ, Liang YC, Lin SY, Lin YS, Wu WC, Hou WC, et al. Antihypertensive activities of a solid-state culture of Taiwanofungus camphoratus (Chang-Chih) in spontaneously hypertensive rats. Biosci Biotech Bioch. 2007;71:23-30.
[7] 陳勁初、林文鑫、陳清農、許勝傑、黃仕政、陳炎鍊. 台灣特有真菌─樟芝菌絲體之開發. Fung Sci. 2001:16(1,2),7-22.
[8] Yang FC, Yang YH, Lu HC. Enhanced antioxidant and antitumor activities of Antrodia cinnamomea cultured with cereal substrates in solid state fermentation. Biochem Eng J. 2013;78:108-13.
[9] Yang FC, Ma TW, Lee YH. Reuse of citrus peel to enhance the formation of bioactive metabolite-triterpenoid in solid-state fermentation of A. cinnamomea. Biochem Eng J. 2013;78:59-66.
[10] Chan YY, Chang CS, Chien LH, Wu TF. Apoptotic effects of a high performance liquid chromatography (HPLC) fraction of Antrodia camphorata mycelia are mediated by down-regulation of the expressions of four tumor-related genes in human non-small cell lung carcinoma A549 cell. J Ethnopharmacol. 2010;127:652-61.
[11] Chang CY, Lue MY, Pan TM. Determination of adenosine, cordycepin and ergosterol contents in cultivated Antrodia camphorata by HPLC method. J Food Drug Anal. 2005;13:338-42.
[12] Chen Y-W. The Effect of LED Luminance and Substrate Composition on the Metabolic Products of Liquid Cultured Cordyceps Militaris: Ming Chuan University; 2011.
[13] Tseng C-J. Effect of LED Lightening on the Secondary Metabolites Production of Monascus rubber 31542 Liquid Fermentation: Ming Chuan University; 2010.
[14] Chang Y-L. In vivo study on the Anti-Fatigue Health Benefits of Liquid Cultured Taiwanofungus camphoratus: Ming Chuan University; 2013.
[15] C-W Cheng, C-K Chen, Chang C-J, Chen L-Y. Effect of Colour LEDs on Mycelia Growth of Aspergillus ficuum and Phytase Production in Photo-Fermentations. Photochemistry and Photobiology B: Biology. 2012;106:81-6.
[16] 陳淑芳. 台灣保健食品產業現況分析與趨勢. 農業生技產業季刊. 2009.
[17] 羅正仁. 綜論台灣保健食品產業發展現況與方向. 食品工業月刊. 2008.
[18] Huang HC, Liaw CC, Yang HL, Hseu YC, Kuo HT, Tsai YC, et al. Lanostane triterpenoids and sterols from Antrodia camphorata. Phytochemistry. 2012;84:177-83.
[19] Chang TT, Chou WN. Antrodia cinnamomea sp. nov. on Cinnamomum kanehirai in Taiwan. Mycol Res. 1995;99:756–8.
[20] Du YC, Wu TY, Chang FR, Lin WY, Hsu YM, Cheng FT, et al. Chemical profiling of the cytotoxic triterpenoid-concentrating fraction and characterization of ergostane stereo-isomer ingredients from Antrodia camphorata. J Pharmaceut Biomed. 2012;58:182-92.
[21] Zang M, Su Q. Ganoderma comphoratum, a new taxon in genus Ganoderma from Taiwan. China Acta Bot Yunnanica. 1990:12:395-6.
[22] Wu SH, Yu ZH, Dai YC, Chen CT, Su CH, Chen LC, et al. Taiwanofungus, a polypore new genus. Fung Sci. 2004:19: 109-16.
[23] Yu ZH, Wu SH, Wang DM, Chen CT. Phylogenetic relationships of Antrodia species and related taxa based on analyses of nuclear large subunit ribosomal DNA sequences. Bot Stud. 2010;51:53-60.
[24] Chen CC, Chyau CC, Hseu TH. Production of a COX-2 inhibitor, 2,4,5-trimethoxybenzaldehyde, with submerged cultured Antrodia camphorata. Lett Appl Microbiol. 2007;44:387-92.
[25] Lin YW, Chiang BH. 4-Acetylantroquinonol B Isolated from Antrodia cinnamomea Arrests Proliferation of Human Hepatocellular Carcinoma HepG2 Cell by Affecting p53, p21 and p27 Levels. J Agr Food Chem. 2011;59:8625-31.
[26] Song TY, Hsu SL, Yeh CT, Yen GC. Mycelia from Antrodia camphorata in submerged culture induce apoptosis of human hepatoma HepG2 cells possibly through regulation of Fas pathway. J Agr Food Chem. 2005;53:5559-64.
[27] Hsieh YC, Rao YK, Wu CC, Huang CYF, Geethangili M, Hsu SL, et al. Methyl Anteinate A from Antrodia camphorata Induces Apoptosis in Human Liver Cancer Cells through Oxidant-Mediated Cofillin- and Bax-Triggered Mitochondrial Pathway. Chem Res Toxicol. 2010;23:1256-67.
[28] Wu H, Pan CL, Yao YC, Chang SS, Li SL, Wu TF. Proteomic analysis of the effect of Antrodia camphorata extract on human lung cancer A549 cell. Proteomics. 2006;6:826-35.
[29] Chen YY, Liu FC, Chou PY, Chien YC, Chang WSW, Huang GJ, et al. Ethanol Extracts of Fruiting Bodies of Antrodia cinnamomea Suppress CL1-5 Human Lung Adenocarcinoma Cells Migration by Inhibiting Matrix Metalloproteinase-2/9 through ERK, JNK, p38, and PI3K/Akt Signaling Pathways. Evid-Based Compl Alt. 2012.
[30] Chiou JF, Wu ATH, Wang WT, Kuo TH, Gelovani JG, Lin IH, et al. A Preclinical Evaluation of Antrodia camphorata Alcohol Extracts in the Treatment of Non-Small Cell Lung Cancer Using Non-Invasive Molecular Imaging. Evid-Based Compl Alt. 2011:1-12.
[31] Chen YJ, Chou CJ, Chang TT. Compound MMH01 possesses toxicity against human leukemia and pancreatic cancer cells. Toxicol in Vitro. 2009;23:418-24.
[32] Liu FC, Lai MT, Chen YY, Lin WH, Chang SJ, Sheu MJ, et al. Elucidating the inhibitory mechanisms of the ethanolic extract of the fruiting body of the mushroom Antrodia cinnamomea on the proliferation and migration of murine leukemia WEHI-3 cells and their tumorigenicity in a BALB/c allograft tumor model. Phytomedicine. 2013;20:874-82.
[33] Hseu YC, Yang HL, Lai YC, Lin JG, Chen GW, Chang YH. Induction of apoptosis by Antrodia camphorata in human premyelocytic leukemia HL-60 cells. Nutr Cancer. 2004;48:189-97.
[34] Chiang SS, Wang LT, Chen SY, Mau JL. Antibacterial and Anti-Inflammatory Activities of Mycelia of a Medicinal Mushroom from Taiwan, Taiwanofungus salmoneus (Higher Basidiomycetes). Int J Med Mushrooms. 2013;15:39-47.
[35] Deng JS, Huang SS, Lin TH, Lee MM, Kuo CC, Sung PJ, et al. Analgesic and Anti-Inflammatory Bioactivities of Eburicoic Acid and Dehydroeburicoic Acid Isolated from Antrodia camphorata on the Inflammatory Mediator Expression in Mice. J Agr Food Chem. 2013;61:5064-71.
[36] Hseu YC, Wu FY, Wu JJ, Chen JY, Chang WH, Lu FJ, et al. Anti-inflammatory potential of Antrodia Camphorata through inhibition of NOS, COX-2 and cytokines via the NF-kappa B pathway. Int Immunopharmacol. 2005;5:1914-25.
[37] Song TY, Yen GC. Protective effects of fermented filtrate from Antrodia camphorata in submerged culture against CCl4-induced hepatic toxicity in rats. J Agr Food Chem. 2003;51:1571-7.
[38] Yu WG, Qian J, Lu YH. Hepatoprotective Effects of 2 '',4 ''-Dihydroxy-6 ''-methoxy-3 '',5 ''-dimethylchalcone on CCl4-Induced Acute Liver Injury in Mice. J Agr Food Chem. 2011;59:12821-9.
[39] Ao ZH, Xu ZH, Lu ZM, Xu HY, Zhang XM, Dou WF. Niuchangchih (Antrodia camphorata) and its potential in treating liver diseases. J Ethnopharmacol. 2009;121:194-212.
[40] Cha WS, Ding JL, Choi D. Comparative evaluation of antioxidant, nitrite scavenging, and antitumor effects of Antrodia camphorata extract. Biotechnol Bioproc E. 2009;14:232-7.
[41] Hsiao G, Shen MY, Lin KH, Lan MH, Wu LY, Chou DS, et al. Antioxidative and hepatoprotective effects of Antrodia camphorata extract. J Agr Food Chem. 2003;51:3302-8.
[42] Wang GJ, Lin SY, Wu WC, Hou WC. DPPH radical scavenging and semicarbazide-sensitive amine oxidase inhibitory and cytotoxic activities of Taiwanofungus camphoratus (Chang-chih). Biosci Biotech Bioch. 2007;71:1873-8.
[43] Cheng PC, Hsu CY, Chen CC, Lee KM. In vivo immunomodulatory effects of Antrodia camphorata polysaccharides in a T1/T2 doubly transgenic mouse model for inhibiting infection of Schistosoma mansoni. Toxicol Appl Pharm. 2008;227:291-8.
[44] Huang CH, Chang CC, Lin CM, Wang ST, Wu MT, Li EIC, et al. Promoting effect of Antrodia camphorata as an immunomodulating adjuvant on the antitumor efficacy of HER-2/neu DNA vaccine. Cancer Immunol Immun. 2010;59:1259-72.
[45] Yang SM, Ka SM, Hua KF, Wu TH, Chuang YP, Lin YW, et al. Antroquinonol mitigates an accelerated and progressive IgA nephropathy model in mice by activating the Nrf2 pathway and inhibiting T cells and NLRP3 inflammasome. Free Radical Bio Med. 2013;61:285-97.
[46] Sheu SenJe CC, Chen ChinChu. Study on the host specificity of Antrodia camphorata. Taiwanese Journal of Agricultural Chemistry and Food Science. 2000;38:533-9.
[47] Xia YJ, Zhang BR, Li WJ, Xu GR. Changes in volatile compound composition of Antrodia camphorata during solid state fermentation. J Sci Food Agr. 2011;91:2463-70.
[48] Zhang H, Xia YJ, Wang YL, Zhang BB, Xu GR. Coupling use of surfactant and in situ extractant for enhanced production of Antrodin C by submerged fermentation of Antrodia camphorata. Biochem Eng J. 2013;79:194-9.
[49] Chang CY, Lee CL, Pan TM. Statistical optimization of medium components for the production of Antrodia cinnamomea AC0623 in submerged cultures. Appl Microbiol Biot. 2006;72:654-61.
[50] Lu ZM, Lei JY, Xu HY, Shi JS, Xu ZH. Optimization of fermentation medium for triterpenoid production from Antrodia camphorata ATCC 200183 using artificial intelligence-based techniques. Appl Microbiol Biot. 2011;92:371-9.
[51] He YC, He KZ, Pu Q, Li J, Zhao ZJ. Optimization of Cultivating Conditions for Triterpenoids Production from Antrodia cinnmomea. Indian J Microbiol. 2012;52:648-53.
[52] Shih IL, Pan K, Hsieh CY. Influence of nutritional components and oxygen supply on the mycelial growth and bioactive metabolites production in submerged culture of Antrodia cinnamomea. Process Biochem. 2006;41:1129-35.
[53] Huang GJ, Deng JS, Huang SS, Shao YY, Chen CC, Kuo YH. Protective effect of antrosterol from Antrodia camphorata submerged whole broth against carbon tetrachloride-induced acute liver injury in mice. Food Chem. 2012;132:709-16.
[54] Svetlana Medvedeva KA, Anastasia Krivosheenko, Anastasia Rusinova, Emma Rodicheva, Alexey Puzyr, Vladimir Bondar. Growth and the Light Emission of Luminous Higher Fungi Cultivated on Solid Media of Different Compositions and in Submerged Culture. microorganisms. 2013.
[55] Tzeng YM, Geethangili M, Ling ST. Purification of bioactive compounds from Antrodia camphorata and their pharmacological activities. J Biosci Bioeng. 2009;108:S23-S.
[56] Huang CC, Hsu MC, Huang WC, Yang HR, Hou CC. Triterpenoid-Rich Extract from Antrodia camphorata Improves Physical Fatigue and Exercise Performance in Mice. Evid-Based Compl Alt. 2012.
[57] Yue PYK, Wong YY, Chan TYK, Law CKM, Tsoi YK, Leung KSY. Review of Biological and Pharmacological Activities of the Endemic Taiwanese Bitter Medicinal Mushroom, Antrodia camphorata (M. Zang et C. H. Su) Sh. H. Wu et al. (Higher Basidiomycetes). Int J Med Mushrooms. 2012;14:241-56.
[58] Chen YC, Chiu HL, Chao CY, Lin WH, Chao LK, Huang GJ, et al. New Anti-Inflammatory Aromatic Components from Antrodia camphorata. Int J Mol Sci. 2013;14:4629-39.
[59] 李宛蓁. 樟芝菌絲體培養與生理活性成分生成之研究. 東海大學化學工程學研究所碩士論文. 2003.
[60] 李順來. 牛樟芝三萜類的結構與生化活性. 2010.
[61] Shen YC, Wang YH, Chou YC, Chen CF, Lin LC, Chang TT, et al. Evaluation of the anti-inflammatory activity of zhankuic acids isolated from the fruiting bodies of Antrodia camphorata. Planta Med. 2004;70:310-4.
[62] Chen YC, Liu YL, Li FY, Chang CL, Wang SY, Lee KY, et al. Antcin A, a steroid-like compound from Antrodia camphorata, exerts anti-inflammatory effect via mimicking glucocorticoids. Acta Pharmacol Sin. 2011;32:904-11.
[63] Wang GJ, Tseng HW, Chou CJ, Tsai TH, Chen CT, Lu MK. The vasorelaxation of Antrodia camphorata mycelia: Involvement of endothelial Ca2+-NO-cGMP pathway. Life Sci. 2003;73:2769-83.
[64] Lu MC, El-Shazly M, Wu TY, Du YC, Chang TT, Chen CF, et al. Recent research and development of Antrodia cinnamomea. Pharmacol Therapeut. 2013;139:124-56.
[65] Park DK, Park HJ. Ethanol Extract of Antrodia camphorata Grown on Germinated Brown Rice Suppresses Inflammatory Responses in Mice with Acute DSS-Induced Colitis. Evid-Based Compl Alt. 2013.
[66] Huang GJ, Pan CH, Liu FC, Wu TS, Wu CH. Anti-inflammatory effects of ethanolic extract of Antrodia salmonea in the lipopolysaccharide-stimulated RAW246.7 macrophages and the lambda-carrageenan-induced paw edema model. Food Chem Toxicol. 2012;50:1485-93.
[67] Shu CH, Lung MY. Effect of pH on the production and molecular weight distribution of exopolysaccharide by Antrodia camphorata in batch cultures. Process Biochem. 2004;39:931-7.
[68] Kuo MC, Chang CY, Cheng TL, Wu MJ. Immunomodulatory effect of Antrodia camphorata mycelia and culture filtrate. J Ethnopharmacol. 2008;120:196-203.
[69] Lin ES, Chen YH. Factors affecting mycelial biomass and exopolysaccharide production in submerged cultivation of Antrodia cinnamomea using complex media. Bioresource Technol. 2007;98:2511-7.
[70] Hasko G, Linden J, Cronstein B, P P. Adenosine receptors: therapeutic aspects for inflammatory and immune diseases. Nat Rev Drug Discov. 2008: 7(9): 759–70.
[71] Chang CH, Wang HE, Liaw PY, Peng CC, Peng RY. Antrodia cinnamomea Exhibits a Potent Neuroprotective Effect in the PC12 Cell-A beta(25-35) Model - Pharmacologically through Adenosine Receptors and Mitochondrial Pathway. Planta Med. 2012;78:1813-23.
[72] M A Trevethick, S J Mantell, E F Stuart, A Barnard, K N Wright, Yeadon M. Treating lung inflammation with agonists of the adenosine A2A receptor: promises, problems and potential solutions. Br J Pharmacol. 2008:155(4): 463–74.
[73] Tang Y, Zhong J. Fed-batch fermentation of Ganoderma lucidum for hyperproduction of polysaccharide and ganoderic acid. Microbial Technology 2002:31 : 20–8.
[74] Liang-Yu Chen, Anren Hu, Chang C-J. The Degradation Mechanism of Toxic Atractyloside in Herbal Medicines by Decoction. Molecules. 2013;18:2018-28.
[75] Wei-Sheng Lin, Jun-Yi Chen, Jo-Chiao Wang, Liang-Yu Chen, Che-Hao Lin, Tsung-Ren Hsieh, et al. The anti-aging effects of Ludwigia octovalvis on Drosophila melanogaster and SAMP8 mice. AGE. 2014;36:689-703.
[76] Chien-Hua Chu, Chi-Ming Chiu, Anren Hu, Hui-Chung Wu, Shu-Ping Ye, Kuo-Chieh Ho, et al. Toxicity attenuation of atractyloside in traditional Chinese medicinal herbs after hydrothermal processing. Bot Stud. 2012;53:459-65.
[77] YC L, HH C, CL C, CH L, JI Y, MF C, et al. Role of perivascular adipose tissue-derived methyl palmitate in vascular tone regulation and pathogenesis of hypertension. Circulation. 2011;124(10):1160-71.
[78] 顧翼東. 化學詞典. 2003.
[79] Yazawa Y YM, Sugiyama K. Antitumor promoting effect of an active component of Polyporus, ergosterol and related compounds on rat urinary bladder carcinogenesis in a short-term test with concanavalin A. Biol Pharm Bull. 2000:11 (): 1298–302.
[80] Takaku TK, Y; Okuda, H. Isolation of an antitumor compound from Agaricus blazei Murill and its mechanism of action. The Journal of nutrition. 2001:131 (5): 1409–13.
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