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研究生:羅宇浩
研究生(外文):YU-HAO LUO
論文名稱:特定牛樟芝子實體純化物對人類前列腺癌細胞在動物實驗內之細胞凋亡機制探討
論文名稱(外文):The apoptotic mechanism of specific compound purified from Antrodia cinnamomea’s fruiting body in prostate carcinoma xenograft model
指導教授:吳宗正吳宗正引用關係
指導教授(外文):Tzong-Zeng Wu
學位類別:碩士
校院名稱:國立東華大學
系所名稱:生命科學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
論文頁數:134
中文關鍵詞:前列腺癌牛樟芝抗腫瘤活體實驗異種移植內源性凋亡路徑外源性凋亡路徑p53
外文關鍵詞:prostate cancerAntrodia cinnamomeaanti-cancerin vivoxenograftIntrinsic apoptosis pathwayextrinsic apoptosis pathwayp53
相關次數:
  • 被引用被引用:2
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  • 下載下載:17
  • 收藏至我的研究室書目清單書目收藏:1
前列腺癌是男性中年後容易罹患的癌症之一,由於飲食習慣的西化,導致前列腺癌的發生率及死亡率逐年增高。牛樟芝為一種獨特的真菌植物,僅生長於陰暗、腐朽的牛樟樹,而牛樟樹為產量稀少、珍貴為台灣特有保育植物。牛樟芝含有豐富的三萜類、多醣體等,具有多種藥理活性如:抗腫瘤、保肝、抗氧化等。本實驗目的是在體外 ( in vitro ) 及活體實驗 ( in vivo ) 中探討牛樟芝子實體分離物,對於抑制前列腺癌細胞生長的分子作用機制。實驗主要分為三部分,首先,篩選出能有效抑制前列腺癌細胞生長的特定牛樟芝子實體純化物。利用MTT assay篩選牛樟芝子實體粗萃物 FK1 ~ FK6 ,其中以 FK5 抑制效果最好,對於前列腺癌細胞株 PC-3, LNCaP 半抑制率分別約為70.36 μg/ml 及52.95 μg/ml ,再將 FK5以 HPLC 半製備分離純化出 K5-2 ,其抑制 PC-3, LNCaP 半抑制率皆約為2.5 μg/ml ;接著測試 K5-2 對正常細胞株 CCD-966SK 之毒性,結果顯示 K5-2 濃度10 μg/ml以下不會抑制其生長;以顯微鏡觀察發現 K5-2 處理後,細胞出現皺縮以及許多碎片產生,再藉由流式細胞儀分析, K5-2 使 LNCaP 細胞週期停滯在SubG1,但PC-3沒有顯著改變,而 PC-3 及LNCaP 皆因 K5-2 處理而有凋亡產生。第二部分利用動物模型觀察牛樟芝子實體分離物在活體內對於腫瘤影響。異種移植前列腺癌細胞 PC-3 誘導免疫缺陷小鼠 ( NOD-SCID ) 長出前列腺腫瘤,以腹腔注射 ( i.p. ) K5-2 ,在濃度10 mg/kg 下,能有效抑制腫瘤體積及重量,接著在組織上以 TUNEL Assay 觀察到隨K5-2 濃度提高,細胞凋亡比例跟著增加。最後利用腫瘤萃取得到之蛋白進行細胞凋亡途徑之分析。 K5-2 會使前列腺癌細胞經由內源性及外源性凋亡途徑, DR4/DR5 及Fas 這些死亡受器表現量增加, Caspase-8 被活化,抑制Bcl-2表現並活化Bax 及Bad ,使粒線體內 Cythchrome C 釋出活化下游 Caspase-9 ,而後 Caspase-3 被活化走向凋亡,同時抑制 PARP 修復 DNA 之能力,增加 p53 表現,間接導致細胞凋亡。 由以上結果可知, K5-2 有很好的潛力當作治療前列腺癌之菇菌來源藥物,且不會對正常細胞造成傷害。
Prostate cancer is one of the susceptible diseases observed among older men. Due to the gradual westernization of dietary and lifestyle habits, leading to prostate cancer incidence and mortality rates increased year by year. Antrodia cinnamomea (AC) is an unique mushroom that grows in dark and humid conditions, and it specifically prefers to grow on the inner caving of the endemic species Cinnamomum kanehirae ( Bull camphor tree ), which is a very rare and one of conservation plants in Taiwan. AC contains abundant triterpenoids and polysaccharides, lot of previous researches have shown that AC has many pharmacological effects such as anti-cancer, hepatoprotective, and anti-oxidant, etc. The purpose of this study was to investigate the cytotoxic effect and molecular mechanism of AC’s bioactive components in prostate cancer cell lines both in vitro and in vivo with xenograft mode. This study was mainly divided into three parts: first, the main aim is to screen out the target compound from AC's fruiting body which can effectively inhibit the growth of prostate cancer cells. The results of MTT assay showed that FK5 expressed best inhibitory effect (IC50 70.36 μg / ml and 52.95 μg / ml in PC-3 and LNCaP, respectively) among all test fractions (FK1 to FK6). After that, K5-2 was further purified from FK5 by using semi-preparative HPLC column, which inhibits both PC-3 and LNCaP with IC50 were approximately 2.5 μg / ml but no toxicity to the normal cell CCD-966SK up to the concentration of 10 μg / ml. Microscopic examination found that many changes in cell morphology such as shrinkage and fragmentation after K5-2 treatment. Flow cytometry analysis showed that LNCaP’s after K5-2 treatment resulted in cell cycle arrested at SubG1 phase, but no significant change in PC-3. However, Annexin-V FITC & PI double staining showed that the percentage of apoptotic cells of PC-3 and LNCaP were increased with the concentration of K5-2 and reached a maximum at 5 μg / ml. The second part of this study was to investigate the effect of K5-2 on PC-3 cells in vivo by using xenograft in NOD-SCID mice to induce prostate tumors grow. Intraperitoneal injected series dosages of K5-2 in NOD-SCID mice; the results showed that K5-2 could effectively suppress the tumor volume and mass at concentration level of 10 mg/kg. Subsequently, TUNEL Assay showed that the proportion of apoptotic cells increased with K5-2 in dose-dependent manner. Finally, to elucidate the apoptotic pathway by using protein extracts from homogenized tumor tissue, Western blot results showed that K5-2 induced apoptosis both associated with intrinsic and extrinsic pathway. The results were summary as followings: both DR4 / DR5 and Fas death receptors were triggered and Caspase-8, -3 were activated after K5-2 treated. Down-regulation of Bcl-2 and up-regulation of Bax, Bad were observed along with the release of Cytochrome C. Subsequently, the level of Caspase-9, -3 were activated which induced apoptosis. The activation of Caspase-3 suppressed the PARP level which could repair DNA. Furthermore, the up-regulation of p53 was observed, which indirectly impacted apoptosis. Therefore, our results suggest that K5-2 may have a great potential to develop as a drug originating from medicinal fungus AC for prostate cancer treatment and will not damage normal.
目錄
中文摘要 I
Abstract III
目錄 V
圖目錄 IXX
表目錄 XV
附錄目錄 XVIII
第壹章、前言 1
第貳章、文獻回顧 3
一、 前列腺癌 3
A. 前列腺構造與特性 3
B. 前列腺癌 4
C. 前列腺癌之診斷 5
D. 前列腺癌之分期 6
E. 前列腺癌治療 7
二、 牛樟芝 9
A. 分類與命名 9
B. 生長環境與型態 10
C. 一般成分在菌絲體與子實體之差異 11
D. 活性成分 13
E. 藥理作用 18
三、 細胞凋亡 22
A. 細胞凋亡 22
B. 細胞凋亡路徑 23
第參章、研究目的 25
第肆章、實驗架構 27
第伍章、實驗材料與方法 29
一、 牛樟芝子實體樣本製備 29
A. 牛樟芝子實體萃取 29
B. 管柱層析法 29
C. HPLC半製備分離、純化與樣本保存 30
D. HPLC分析 31
二、 細胞實驗 ( in vitro ) 31
A. 前列腺癌細胞培養與繼代 31
B. 前列腺癌細胞存活率測試 34
C. 前列腺癌細胞週期分析 35
D. 前列腺癌細胞凋亡測試 36
三、 動物實驗 ( in vivo ) 38
A. 實驗動物 38
B. 實驗方法 38
C. 病理組織切片 40
D. 西方墨點法 ( Western ) 41
E. 統計分析 43
第陸章、實驗結果 45
一、 牛樟芝子實體樣本製備 45
A. 牛樟芝子實體萃取 45
B. 管柱層析 45
C. FK5 半製備 HPLC 純化分離 45
D. 半製備 HPLC 純化分離 K5-2 45
二、 細胞實驗 ( in vitro ) 46
A. 細胞存活率測試 46
B. 細胞形態變化 49
C. 細胞週期測試 50
D. 細胞凋亡測試 51
三、 動物實驗 ( in vivo ) 53
A. K5-2 處理對於小鼠體重之影響 53
B. K5-2 處理後腫瘤體積與重量 54
C. 腫瘤組織切片 54
D. K5-2 處理之腫瘤萃取蛋白探討細胞凋亡之訊息傳遞 55
第柒章、討論 103
第捌章、結論 115
第玖章、未來展望 117
第拾章、參考文獻 119

Bahn, D. K. & Silverman, P. D. 2008. Focal cryoablation of prostate: a review. The Scientific World Journal, 8, 486-491.
Bergan, R. C., Reed, E., Myers, C. E., Headlee, D., Brawley, O., Cho, H. K., Figg, W. D., Tompkins, A., Linehan, W. M. & Kohler, D. 1999. A phase II study of high-dose tamoxifen in patients with hormone-refractory prostate cancer. Clinical cancer research, 5, 2366-2373.
Bhuiyan, M. M., Li, Y., Banerjee, S., Ahmed, F., Wang, Z., Ali, S. & Sarkar, F. H. 2006. Down-regulation of androgen receptor by 3, 3′-diindolylmethane contributes to inhibition of cell proliferation and induction of apoptosis in both hormone-sensitive LNCaP and insensitive C4-2B prostate cancer cells. Cancer research, 66, 10064-10072.
Boatright, K. M. & Salvesen, G. S. 2003. Mechanisms of caspase activation. Current opinion in cell biology, 15, 725-731.
Boulares, A. H., Yakovlev, A. G., Ivanova, V., Stoica, B. A., Wang, G., Iyer, S. & Smulson, M. 1999. Role of poly (ADP-ribose) polymerase (PARP) cleavage in apoptosis Caspase 3-resistant PARP mutant increases rates of apoptosis in transfected cells. Journal of Biological Chemistry, 274, 22932-22940.
Catalona, W., Richie, J., Ahmann, F., Hudson, M., Scardino, P., Flanigan, R., De, Dekernion, J., Ratliff, T., Kavoussi, L. & Dalkin, B. 1994. Comparison of digital rectal examination and serum prostate specific antigen in the early detection of prostate cancer: results of a multicenter clinical trial of 6,630 men. The Journal of urology, 151, 1283-1290.
Chang, C. Y., Huang, Z. N., Yu, H. H., Chang, L. H., Li, S. L., Chen, Y. P., Lee, K. Y. & Chuu, J. J. 2008. The adjuvant effects of Antrodia Camphorata extracts combined with anti-tumor agents on multidrug resistant human hepatoma cells. Journal of Ethnopharmacology, 118, 387-395.
Chang, T. T. & Chou, W. N. 1995. Antrodia cinnamomea sp. nov. on Cinnamomum kanehirai in Taiwan. Mycological Research, 99, 756-758.
Chen, J. J., Lin, W. J., Liao, C. H & Shieh, P. C. 2007a. Anti-inflammatory benzenoids from Antrodia camphorata. Journal of natural products, 70, 989-992.
Chen, K. C., Peng, C. C., Peng, R. Y., Su, C. H., Chiang, H. S., Yan, J. H. & Hsieh-Li, H. M. 2007b. Unique formosan mushroom Antrodia camphorata differentially inhibits androgen-responsive LNCaP and-independent PC-3 prostate cancer cells. Nutrition and cancer, 57, 111-121.
Chen, Y. J., Chou, C. J. & Chang, T. T. 2009. Compound MMH01 possesses toxicity against human leukemia and pancreatic cancer cells. Toxicology in Vitro, 23, 418-424.
Chendil, D., Ranga, R. S., Meigooni, D., Sathishkumar, S. & Ahmed, M. M. 2004. Curcumin confers radiosensitizing effect in prostate cancer cell line PC-3. Oncogene, 23, 1599-1607.
Cherng, I. H., Chiang, H. C., Cheng, M. C. & Wang, Y. 1995. Three new triterpenoids from Antrodia cinnamomea. Journal of Natural products, 58, 365-371.
De Almagro, M. & Vucic, D. 2012. The inhibitor of apoptosis (IAP) proteins are critical regulators of signaling pathways and targets for anti-cancer therapy. Exp Oncol, 34, 200-211.
Decaudin, D., Marzo, I., Brenner, C. & Kroemer, G. 1998. Mitochondria in chemotherapy-induced apoptosis: a prospective novel target of cancer therapy (review). International journal of oncology, 12, 141-193.
Dewson, G. & Kluck, R. M. 2009. Mechanisms by which Bak and Bax permeabilise mitochondria during apoptosis. Journal of cell science, 122, 2801-2808.
Dietrich, W., Susani, M., Stifter, L. & Haitel, A. 2011. The Human Female Prostate—Immunohistochemical Study with Prostate-Specific Antigen, Prostate-Specific Alkaline Phosphatase, and Androgen Receptor and 3-D Remodeling. The Journal of Sexual Medicine, 8, 2816-2821.
Dorai, T., Cao, Y. C., Dorai, B., Buttyan, R. & Katz, A. E. 2001. Therapeutic potential of curcumin in human prostate cancer. III. Curcumin inhibits proliferation, induces apoptosis, and inhibits angiogenesis of LNCaP prostate cancer cells in vivo. The prostate, 47, 293-303.
Edge, S. B. & Compton, C. C. 2010. The American Joint Committee on Cancer: the 7th edition of the AJCC cancer staging manual and the future of TNM. Annals of surgical oncology, 17, 1471-1474.
Edinger, A. L. & Thompson, C. B. 2004. Death by design: apoptosis, necrosis and autophagy. Current opinion in cell biology, 16, 663-669.
Epstein, J. I., Allsbrook Jr, W. C., Amin, M. B., Egevad, L. L. & Committee, I. G. 2005. The 2005 International Society of Urological Pathology (ISUP) consensus conference on Gleason grading of prostatic carcinoma. The American journal of surgical pathology, 29, 1228-1242.
Epstein, J. I., Partin A. W., Sauvageot, J. & Walsh, P. C. 1996. Prediction of progression following radical prostatectomy: a multivariate analysis of 721 men with long-term follow-up. The American journal of surgical pathology, 20, 286-292.
Fulda, S. & Debatin, K. M. 2006. Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy. Oncogene, 25, 4798-4811.
Geethangili, M. & Tzeng, Y. M. 2011a. Review of pharmacological effects of Antrodia camphorata and its bioactive compounds. Evidence-Based Complementary and Alternative Medicine, 2011.
Geethangili, M. & Tzeng, Y. M. 2011b. Review of Pharmacological Effects of Antrodia camphorata and Its Bioactive Compounds. Evid Based Complement Alternat Med, 2011, 212641.
Green, D. R. & Kroemer, G. 2004. The pathophysiology of mitochondrial cell death. Science, 305, 626-629.
Hirotani, M. & Furuya, T. 1990. Changes of the triterpenoid patterns during formation of the fruit body in Ganoderma lucidum. Phytochemistry, 29, 3767-3771.
Horoszewicz, J. S., Leong, S. S., Kawinski, E., Karr, J. P., Rosenthal, H., Chu, T. M., Mirand, E. A. & Murphy, G. P. 1983. LNCaP model of human prostatic carcinoma. Cancer research, 43, 1809-1818.
Hotz, M. A., Bosq, J., Zbaeren, P., Reed, J., Schwab, G., Krajewski, S., Brousset, P. & Borner, M. M. 1999. Spontaneous apoptosis and the expression of p53 and Bcl-2 family proteins in locally advanced head and neck cancer. Arch Otolaryngol Head Neck Surg, 125, 417-22.
Hsiao, G., Shen, M.-Y., Lin, K. H., Lan, M. H., Wu, L. Y., Chou, D. S., Lin, C. H., Su, C. H. & Sheu, J. R. 2003. Antioxidative and hepatoprotective effects of Antrodia camphorata extract. Journal of Agricultural and Food Chemistry, 51, 3302-3308.
Hsu, Y. L., Kuo, Y. C., Kuo, P. L., Ng, L. T., Kuo, Y. H. & Lin, C. C. 2005. Apoptotic effects of extract from Antrodia camphorata fruiting bodies in human hepatocellular carcinoma cell lines. Cancer letters, 221, 77-89.
Ito, M., Hiramatsu, H., Kobayashi, K., Suzue, K., Kawahata, M., Hioki, K., Ueyama, Y., Koyanagi, Y., Sugamura, K. & Tsuji, K. 2002. NOD/SCID/γ mouse: an excellent recipient mouse model for engraftment of human cells. Blood, 100, 3175-3182.
Joslyn, S. A. & Konety, B. R. 2006. Impact of extent of lymphadenectomy on survival after radical prostatectomy for prostate cancer. Urology, 68, 121-125.
Kandala, P. K. & Srivastava, S. K. 2012. Diindolylmethane suppresses ovarian cancer growth and potentiates the effect of cisplatin in tumor mouse model by targeting signal transducer and activator of transcription 3 (STAT3). BMC medicine, 10, 9.
Kastan, M. B. & Bartek, J. 2004. Cell-cycle checkpoints and cancer. Nature, 432, 316-323.
Kumar, V. B., Yuan, T. C., Liou, J. W., Yang, C. J., Sung, P. J. & Weng, C. F. 2011. Antroquinonol inhibits NSCLC proliferation by altering PI3K/mTOR proteins and miRNA expression profiles. Mutat Res, 707, 42-52.
Kundra, V., Silverman, P. M., Matin, S. F. & Choi, H. 2007. Imaging in Oncology from The University of Texas M. D. Anderson Cancer Center: Diagnosis, Staging, and Surveillance of Prostate Cancer. American Journal of Roentgenology, 189, 830-844.
Langeler, E. G., Van Uffelen, C. J., Blankenstein, M. A., Van Steenbrugge, G. J. & Mulder, E. 1993. Effect of culture conditions on androgen sensitivity of the human prostatic cancer cell line LNCaP. The Prostate, 23, 213-223.
Lee, I., Huang, R. L., Chen, C. T., Chen, H. C., Hsu, W. C. & Lu, M. K. 2002. Antrodia camphorata polysaccharides exhibit anti-hepatitis B virus effects. FEMS Microbiology Letters, 209, 63-67
Leibel, S. A., Kutcher, G. J., Mohan, R., Harrison, L. B., Armstrong, J. G., Zelefsky, M. J., Losasso, T. J., Burman, C. M., Mageras, G. S. & Chui, C. S. Three-dimensional conformal radiation therapy at the Memorial Sloan-Kettering Cancer Center. Seminars in radiation oncology, 1992. Elsevier, 274-289.
Lin, C. C., Hsu, C. H., Chen, J., Tsai, T. C., Cheng, A. L. & Pu, Y. S. 2000. A pilot study of AFL-T (doxorubicin, 5-fluorouracil, leucovorin, and tamoxifen) combination chemotherapy for hormone-refractory prostate cancer. Anticancer research, 21, 1385-1390.
Lodish, H. 2008. Molecular cell biology, Macmillan.
Lopes, U. G., Erhardt, P., Yao, R. & Cooper, G. M. 1997. p53-dependent induction of apoptosis by proteasome inhibitors. Journal of Biological Chemistry, 272, 12893-12896.
Lu, Z. M., Tao, W. Y., Zou, X. L., Fu, H. Z. & Ao, Z. H. 2007. Protective effects of mycelia of Antrodia camphorata and Armillariella tabescens in submerged culture against ethanol-induced hepatic toxicity in rats. Journal of ethnopharmacology, 110, 160-164.
Majno, G. & Joris, I. 1995. Apoptosis, oncosis, and necrosis. An overview of cell death. The American journal of pathology, 146, 3.
Makino, M., Shoji, H., Takemoto, D., Honboh, T., Nakamura, S., Kurayoshi, K. & Kaibara, N. 2001. Comparative study between daily and 5-days-a-week administration of oral 5-fluorouracil chemotherapy in mice: determining the superior regimen. Cancer chemotherapy and pharmacology, 48, 370-374.
Mcneal, J. E., Redwine, E. A., Freiha, F. S. & Stamey, T. A. 1988. Zonal distribution of prostatic adenocarcinoma: correlation with histologic pattern and direction of spread. The American journal of surgical pathology, 12, 897-906.
Mu, Z. & Qinghua, S. 1990. Ganoderma comphoratum, a new taxon in genus Ganoderma from Taiwan, China. Acta Botanica Yunnanica, 4, 006.
Nukatsuka, M., Fujioka, A., Saito, H., Uchida, J., Nakano, K., Takeda, S. & Unemi, N. 1996. Prolongation of survival period and improvement of cancer cachexia by long term administration of UFT. Cancer letters, 104, 197-203.
O’Brien, C. A., Pollett, A., Gallinger, S. & Dick, J. E. 2007. A human colon cancer cell capable of initiating tumour growth in immunodeficient mice. Nature, 445, 106-110.
Oh, W. K. & Kantoff, P. W. 1998. Management of hormone refractory prostate cancer: current standards and future prospects. The Journal of urology, 160, 1220-1229.
Ono, M., Inkson, C. A., Sonn, R., Kilts, T. M., De Castro, L. F., Maeda, A., Fisher, L. W., Robey, P. G., Berendsen, A. D., Li, L., Mccartney-Francis, N., Brown, A. C., Crawford, N. P., Molinolo, A., Jain, A., Fedarko, N. S. & Young, M. F. 2013. WISP1/CCN4: a potential target for inhibiting prostate cancer growth and spread to bone. Plos One, 8, e71709.
Ou, Y. H., Chung, P. H., Sun, T. P. & Shieh, S. Y. 2005. p53 C-terminal phosphorylation by CHK1 and CHK2 participates in the regulation of DNA-damage-induced C-terminal acetylation. Molecular biology of the cell, 16, 1684-1695.
Patrawala, L., Calhoun, T., Schneider-Broussard, R., Li, H., Bhatia, B., Tang, S., Reilly, J., Chandra, D., Zhou, J. & Claypool, K. 2006. Highly purified CD44+ prostate cancer cells from xenograft human tumors are enriched in tumorigenic and metastatic progenitor cells. Oncogene, 25, 1696-1708.
Ryan, K. M., Phillips, A. C. & Vousden, K. H. 2001. Regulation and function of the p53 tumor suppressor protein. Current opinion in cell biology, 13, 332-337.
Saelens, X., Festjens, N., Walle, L. V., Van Gurp, M., Van Loo, G. & Vandenabeele, P. 2004. Toxic proteins released from mitochondria in cell death. Oncogene, 23, 2861-2874.
Schuler, M. & Green, D. 2001. Mechanisms of p53-dependent apoptosis. Biochemical Society Transactions, 29, 684-687.
Sgonc, R. & Gruber, J. 1998. Apoptosis detection: an overview. Experimental gerontology, 33, 525-533.
Shen, Y. C., Chou, C. J., Wang, Y. H., Chen, C. F., Chou, Y. C. & Lu, M. K 2004. Anti-inflammatory activity of the extracts from mycelia of Antrodia camphorata cultured with water-soluble fractions from five different Cinnamomum species. FEMS Microbiology Letters, 231, 137-143
Shu, C. H. & Lung, M. Y. 2008. Effect of culture pH on the antioxidant properties of Antrodia camphorata in submerged culture. Journal of the Chinese Institute of Chemical Engineers, 39, 1-8.
Shukla, S., Sharma, H., Abbas, A., Maclennan, G. T., Fu, P., Danielpour, D. & Gupta, S. 2013. Upregulation of SATB1 is associated with prostate cancer aggressiveness and disease progression. Plos one, 8, e53527.
Singh, R. P., Tyagi, A. K., Dhanalakshmi, S., Agarwal, R. & Agarwal, C.
2004. Grape seed extract inhibits advanced human prostate tumor growth and angiogenesis and upregulates insulin-like growth factor biding protein-3. International Journal of Cancer, 108, 733-740.
Song, T. Y., Hsu, S. L., Yeh, C. T. & Yen, G. C. 2005. Mycelia from Antrodia camphorata in submerged culture induce apoptosis of human hepatoma HepG2 cells possibly through regulation of Fas pathway. Journal of agricultural and food chemistry, 53, 5559-5564.
Song, T. Y. & Yen, G. C. 2002. Antioxidant properties of Antrodia camphorata in submerged culture. Journal of Agricultural and Food Chemistry, 50, 3322-3327.
Varfolomeev, E. E. & Ashkenazi, A. 2004. Tumor necrosis factor: an apoptosis JuNKie? Cell, 116, 491-497.
Vermeulen, K., Berneman, Z. N. & Van Bockstaele, D. R. 2003. Cell cycle and apoptosis. Cell proliferation, 36, 165-175.
Wei, J. H., Chou, Y. F., Ou, Y. H., Yeh, Y. H., Tyan, S. W., Sun, T. P., Shen, C. Y. & Shieh, S. Y. 2005. TTK/hMps1 participates in the regulation of DNA damage checkpoint response by phosphorylating CHK2 on threonine 68. Journal of Biological Chemistry, 280, 7748-7757.
Whelan, P. 2000. Multidisciplinary Symposium—Prostate Cancer. Cancer Imaging, 1, 44-51.
Wu, B., Zhang, Q., Shen, W. & Zhu, J. 2008. Anti-proliferative and chemosensitizing effects of luteolin on human gastric cancer AGS cell line. Mol Cell Biochem, 313, 125-32.
Wu, J. 2010. Statistical inference for tumor growth inhibition T/C ratio. J Biopharm Stat, 20, 954-64.
Wu, S. H., Kirk, P. M., Redhead, S. A., Stalpers, J. A., Dai, Y. C., Norvell, L. L., Yang, Z. L., Ryvarden, L., Su, C. H. & Li, Y. 2012. Resolution of the nomenclature for niu-chang-chih (Taiwanofungus camphoratus), an important medicinal polypore. Taxon, 61, 1305-1310.
Wu, S. H. & Ryvarden, L. 1997. Antrodia camphorata (" niu-chang-chih"), new combination of a medicinal fungus in Taiwan. Botanical Bulletin-Academia Sinica Taipei, 38, 273-276.
Wu, S. J., Leu, Y. L., Chen, C. H., Chao, C. H., Shen, D. Y., Chan, H. H., Lee, E. J., Wu, T. S., Wang, Y. H., Shen, Y. C., Qian, K., Bastow, K. F. & Lee, K. H. 2010. Camphoratins A-J, potent cytotoxic and anti-inflammatory triterpenoids from the fruiting body of Taiwanofungus camphoratus. J Nat Prod, 73, 1756-62.
Wyllie, A. H. 1997. Apoptosis: an overview. Br Med Bull, 53, 451-65.
Yang, H., Chen, D., Cui, Q. C., Yuan, X. & Dou, Q. P. 2006. Celastrol, a triterpene extracted from the Chinese “Thunder of God Vine,” is a potent proteasome inhibitor and suppresses human prostate cancer growth in nude mice. Cancer research, 66, 4758-4765.
Yue, P. Y. K., Wong, Y. Y., Chan, T. Y. K., Law, C. K. M., Tsoi, Y. K. & Leung, K. S. Y. 2012. Review of Biological and Pharmacological Activities of the Endemic Taiwanese Bitter Medicinal Mushroom, Antrodia camphorata (M. Zang et Ch Su) Sh. H. Wu et al.(Higher Basidiomycetes). International journal of medicinal mushrooms, 14.
Zins, K., Thomas, A., Lucas, T., Sioud, M., Aharinejad, S. & Abraham, D. 2013. Inhibition of stromal PlGF suppresses the growth of prostate cancer xenografts. Int J Mol Sci, 14, 17958-71.
吳威翰 2012. 牛樟芝子實體萃取物誘導肺癌細胞進行細胞凋亡之研究. 國立東華大學生命科學系生物技術碩士班碩士論文, 1-102.
吳書慧 2013. 特定牛樟芝子實體純化物對人類胃癌細胞株 AGS 之細胞凋亡機制探討. 國立東華大學生命科學系生物技術碩士班碩士論文.
李嘉偉 2011. 牛樟芝子實體活性成分抑制前列腺癌之作用機轉. 國立東華大學生命科學系生物技術碩士班碩士論文, 1-102.
邱年永 & 張光雄 1995. 原色台灣藥用植物圖鑑(6), 台北, 台北南天書局.
高曉薇 1991. 台灣靈芝屬新種樟芝之三帖類成分研究. 台灣醫學院天然物醫學研究所碩士論文.
陳勁初 & 呂鋒洲 2001. 靈芝之王: 台灣樟芝, 元氣齋出版社.
陳勁初, 林文鑫, 陳清農, 許勝傑, 黃仕政 & 陳炎鍊 2001. 台灣特有真菌-樟芝菌絲體之開發. Fungal Science, 16, 7-22.
彭梓涵 2012. 特定牛樟芝子實體純化物對人類卵巢癌細胞株PA-1、OVCAR-3之影響. 國立東華大學生命科學系生物技術碩士班碩士論文.
詹俊彥, 劉建良 & 陳英貞 2011. 攝護腺癌之診斷與治療. 家庭醫學與基層醫療, 26, 201-208.
歐貴仁 2006. 樟芝的保肝功效與保肝有效成分純化之研究. 成功大學藥理學研究所學位論文, 1-76.
蔡雁暉 2002. 樟芝深層培養液及其多醣體之抗氧化特性. 國立中興大學食品暨應用生物科技學系碩博士論文.
蘇慶華 2002. The utilization of waste from fruiting body of Ganoderma for healing enhancement on human chronic skin ulcer. 臺北醫學大學醫學系復健學科會議論文.

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