跳到主要內容

臺灣博碩士論文加值系統

(18.208.186.139) 您好!臺灣時間:2022/05/29 04:59
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

: 
twitterline
研究生:王志誠
研究生(外文):Wang, Chih-Cheng
論文名稱:探討以新萃取方式之椴木牛樟芝子實體萃取物對護肝功效之分析評估
論文名稱(外文):Evaluation the Hepatoprotection Effects of Wood Cultivated Antrodia Cinnamomea Fruiting Bodies by New Extraction Method
指導教授:郭鐘達
指導教授(外文):Kuo, Jong-Tar
口試委員:林恩仕陳煜沛
口試委員(外文):Lin, En-ShyhChen, Yu-Pei
口試日期:2015-05-30
學位類別:碩士
校院名稱:中華科技大學
系所名稱:健康科技研究所在職專班
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:中文
論文頁數:53
中文關鍵詞:椴木牛樟芝抗氧化活性護肝
外文關鍵詞:Wood Cultivated Antrodia CinnamomeaAntioxidant ActivityHepatoprotective
相關次數:
  • 被引用被引用:2
  • 點閱點閱:614
  • 評分評分:
  • 下載下載:10
  • 收藏至我的研究室書目清單書目收藏:1
本研究為在四氯化碳誘導肝損傷的動物實驗模式下,探討椴木牛樟芝萃取粉末之護肝保健功效。測試物為金盛旺生物科技股份有限公司提供之椴木牛樟芝萃取粉末。在實驗部份,將Sprague Dawley(SD)雄鼠以隨機方式分為控制組、四氯化碳負對照組、silymarin正對照組(200 mg/kg bw)組、一倍劑量(0.44 g/rat)實驗組、二倍劑量(0.88 g/rat)實驗組,以及四倍劑量(0.176 g/rat)實驗組等六組,實驗為期八週。所有實驗動物分別於第零週、第一週、第三週及第六週投予試驗樣品後24小時,以尾部採血方式進行分析。實驗結果顯示,相較於四氯化碳負對照組,三種劑量實驗組皆可以降低血清中AST(GOT)和ALT(GPT),其中以四倍劑量(0.176g/rat/day)實驗組有顯著效果(p <0.05);另外,相較於四氯化碳負對照組,三種劑量亦顯著降低血漿中總膽固醇(TC)以及三酸甘油脂(TG),並也以四倍劑量實驗組有顯著效果(p <0.05)。在抗氧化酵素方面,相較於四氯化碳負對照組,三種劑量實驗組,能顯著改善肝臟中抗氧化物質glutathione(GSH)含量(p<0.05),並提升抗氧化酵素glutathione peroxidase(GPx)、glutathione reductase(GRd)、superoxide dismutase(SOD)、catalase(CAT)等酵素活性,此結果表示能夠改善因CCl4毒性造成肝臟GSH-Px、GSH-Rd、SOD、CAT等酵素活性降低的問題,對肝臟產生抗氧化作用的保護功效。因此,綜合上述實驗結果推測,椴木牛樟芝萃取粉可減少因四氯化碳所造成之肝損傷酵素指標AST(GOT)和ALT(GPT)上升、降低總膽固醇(TC)以及三酸甘油脂(TG),並提升抗氧化酵素活性,達顯著保護效果,故椴木栽培牛樟芝子實體確實具有改善四氯化碳誘導肝損傷之護肝保健潛力。
The aim of this study was to evaluate the hepatoprotective effect of wood cultivated Antrodia Cinnamomea fruiting bodies extract powder on carbon tetrachloride CCl4-induced liver injuries in rats. Grand Fortune Biotechnology Company provided the Wood Cultivated Antrodia cinnamomea Fruiting Bodies extract powder. In this experiment, male Sprague Dawley (SD) rats were randomly divided into six groups: control, CCl4 group, CCl4+silymarin(200 mg/kg bw), CCl4+(0.44g Antrodia Cinnamomea extract powder/rat), CCl4+(0.88 g/rat), CCl4+(0.176 g/rat). The experimental period was 8 weeks. All of the rats drew the blood sample from tail root to proceed biochemical analyses after voted the Antrodia Cinnamomea extract powder in 24 hours at week 0, week 1, week 3 and week 6. The end of the experiment, compared with CCl4 group, the results indicated that the rats in CCl4+(0.44 g/rat), CCl4+(0.88 g/rat) and CCl4+(0.176 g/rat) showed a lower plasma AST(GOT) and ALT(GPT), especially significantly lower in CCl4+(0.176 g/rat) (p <0.05). Besides, compared with CCl4 group, the rats in CCl4+(0.44 g/rat), CCl4+(0.88 g/rat) and CCl4+(0.176 g/rat) showed a lower plasma total cholesterol(TC) and triglyceride(TG), and also especially significantly lower in CCl4+(0.176 g/rat) (p <0.05). Furthermore, compared with CCl4 group, glutathione(GSH) was increased significantly in group CCl4+(0.44 g/rat), CCl4+(0.88 g/rat) and CCl4+(0.176 g/rat) and also improved the activities of hepatic glutathione peroxidase(GSH-Px)、glutathione reductase(GSH-Rd)、superoxide dismutase(SOD)、catalase(CAT). In other words, none of group CCl4+(0.44 g/rat), CCl4+(0.88 g/rat) and CCl4+(0.176 g/rat) could reduce the oxidative injury induced by CCl4 and protection liver. Overall, these results suggest that wood cultivated Antrodia Cinnamomea fruiting bodies extract powder could reduce plasma AST(GOT)、ALT(GPT)、total cholesterol(TC) and triglyceride(TG) , increase hepatic antioxidant enzymes capacity and improve hepatic protection in rats with CCl4-induced liver damage. It has beneficial effects and potential on liver function.
英文摘要 i
中文摘要 ii
目次 iii
表目錄 v
圖目錄 vi
第一章 前言 1
第一節 研究背景 1
第二節 研究目的 2
第二章 文獻回顧 3
第一節 牛樟芝介紹 3
第二節 肝臟介紹 8
第三節 保健食品市場分析 10
第四節 護肝機能性食品分析 16
第五節 食品毒理機制與安全性評估 19
第六節 牛樟芝在台灣保健食品功效分析 21
第三章 材料與方法 24
第一節 實驗材料 24
第二節 實驗分組與流程 27
第三節 測定項目與方法 28
第四章 結果與討論 33
第一節 體重變化情形 33
第二節 每日平均飼料與樟芝產品攝食量 33
第三節 肝功能指標 34
第四節 肝臟抗氧化能力 37
第五節 肝臟組織病理切片 39
第五章 結論 44
第六章 參考文獻 46

王伯徹、黃仁彰(2002),靈芝與樟芝之研究與市場面面觀,食品工業,第34卷,第5期,3-17。
朱建儒(2003),探討通氣量對於樟芝發酵生產生物鹼之影響,國立中央大學化學工程與材料工程研究所碩士論文。
沈立言、陳怡欣、林文川(2001),牛樟芝菌絲體過濾液對肝臟生理機能性之影響,中華民國食品科學技術學會年會,台大食品科技研究所。
李宛蓁(2003),樟芝菌絲體培養與生理活性成分生成之研究,私立東海大學化學工程研究所碩士論文。
李奇翰(2001),靈芝抗癌之效果,國立臺灣大學醫事技術學研究所碩士論文。
李雨薇(2006),牛樟芝菌絲體發酵液對血管新生抑制作用及其機制之探討,長庚大學生化與生醫工程研究所碩士論文。
李貞宜(2005),樟芝保肝作用之探討,南台科技大學生物科技系碩士論文。
宋祖瑩(2003),樟芝深層培養液抗氧化及抗腫瘤特性之研究,國立中興大學食品科學系博士論文。
周傑夫(2012年12月21日),健康護理老來瘋-保健食品&長期照護商機浮現,萬寶周刊,999期。
食工所(2013),2013年食品產業年鑑,經濟部技術處。
食工所(2014),2014年食品產業年鑑,經濟部技術處。
食藥署(2015),衛福部審核通過之健康食品一覽表,食品藥物消費者知識服務網,衛生福利部食品藥物管理署。
陳妙齡、林璧鳳(2007),篩選調節過敏反應食材之細胞培養及小鼠評估方法,台灣農業化學與食品科學,第45卷,第6期,316-326。
陳怡欣(2002),牛樟芝發酵過濾液對大白鼠肝臟生理機能之影響,私立中國醫藥學院營養研究所碩士論文。
陳勁初(2008),樟芝為元氣之寶,台北市:元氣齋出版社有限公司。
陳盈宜、張慧欣、李曉媛、許輔(2008),餵食樟芝子實體對於小鼠體內肝腫瘤細胞之免疫調節與抑制腫瘤效果之探討,台灣農業化學與食品科學,第46卷,第2期,87-95。
陳健弘(2005),找回健康肝,台北市:原水文化事業股份有限公司,4-8。
陳淑芳(2005),我國食品生技產業市場趨勢,農業生技產業季刊,第3期,1-8。
陳啟楨、蘇慶華、藍明煌(2001),樟芝固體栽培及其生物活性之研究,菌類科學,第16卷,第1-2期,65-72。
張東柱、CAC101研發團隊(2010),牛樟芝的神奇療效-保肝抗癌的台灣森林奇蹟,台北市:商周出版。
黃鈴娟(2000),樟芝與姬松茸之抗氧化性質及其多醣組成分析,國立中興大學食品科學系研究所碩士論文。
新橋生技(2015),液態發酵菌絲體,新橋生物科技有限公司,網址http://www.nb-biotech.com/。
楊燿銘(2009),牛樟芝讓生命更豐富,台北市:巨煒股份有限公司。
潘子明(2006),樟芝之生產應用與國內研究現況,生物產業,第17卷,第4期,38-50。
歐貴仁(2005),樟芝的保肝功效與保肝有效成分純化之研究,國立成功大學藥理學研究所碩士論文。
劉千瑈(2006),樟芝樹突狀細胞之免疫調控作用與對巨噬細胞之抗發炎反應,陽明大學熱帶醫學研究所碩士論文。
劉江明、張貴香(2007),肝膽病自我診斷與防治,新北市:新潮社文化事業有限公司。
劉昌峰(2000),探討不同培養基組成對光合菌Rhodobacter sphaeroidesg生產Coenzyme Q10之研究,中央大學化學工程學研究所碩士論文。
劉依蓁(2013),國內肝功能、抗疲勞保健食品簡析,台灣經濟研究院生物科技產業研究中心。
劉彩艷(2010),保肝產品可以保肝嗎,康健雜誌136 期。
劉翠玲(2007),保肝中草藥保健食品市場現況與趨勢,台灣經濟研究院生物科技產業研究中心。
劉翠玲(2009),全球保健食品產業發展現況與展望,農業生技產業季刊,第18期,1-8,。
劉翠玲(2012),從預防醫學的角度出發-全球保健食品產業趨勢,台灣經濟研究月刊,第35卷,第3期。
衛福部(1975),食品衛生管理法,中華民國衛生福利部。
衛福部(1999),健康食品管理法,中華民國衛生福利部。
穀研所(2011),保健食品工業技術推廣與輔導計畫,經濟部工業局。
閻慶松、于志斌(2013),2012年美國草藥類膳食補充劑市場強分析,中國現代中藥,第15卷,第9期,800-804。
戴宇昀、蕭學民、蔡金川、陳勁初、黃仕政、胡淼琳(2001),樟芝子實體對酒精誘發急性肝損傷之保肝功能評估,中華保健食品協會。
顏士榮(2006),樟芝降三酸甘油脂之功能研究,台北醫學大學醫學檢驗生物技術研究所碩士論文。
簡秋源、姜宏哲、陳淑貞(1997),牛樟菇培養性狀及其三萜類成分分析,牛樟芝研討會論文集,133-137。
蘇正德(2006),日本保健食品產業現況與市場發展趨勢,農業生技產業季刊,第7期。
Abou-Assi, S. and Vlahcevic, Z. R. (2001). Hepatic encephalopathy. Metabolic consequence of cirrhosis often is reversible. Postgrad Med 109:52-54, 57-60, 63-65 passim.
Aebi, H. (1984). Catalase. In: L. Packer(Ed), methods in enzymology. Academic pres, Orlando 105: 121-126.
Bellomo, G., Mirabelli, F., DiMonte, D., Richelmi, P., Thor, H., Orrenius, C. and Orrenius, S. (1987). Formation and reduction of glutathione-protein mixed disulfides during oxidative stress. A study with isolated hepatocytes and menadione (2-methyl-1,4-naphthoquinone). Biochem Pharmacol 36:1313-1320.
Beyer, W., Imlay, J. and Fridovich, I. (1991). Superoxide dismutases. Prog Nucleic Acid Res Mol Biol 40:221-253.
Carola, R., Harley, J. P. and Noback, C. R. (1992). Human anatomy and Physiology. McGraw-Hill Inc 709-712.
Chang, T. T. and Chou, W. N. (1995). Antrodia cinnamomea sp. nov. on Cinnamomum Kanehirai in Taiwan. Mycological Research 99:756-758.
Chang, T. T. and Chou, W. N. (2004). Antrodia cinnamomea reconsidered and A. salmonea sp. nov. on Cunninghamia konishii in Taiwan. Botanical Bulletin of the Academia Sinica(Taipei) 45:347-352.
Chen, J. C., Lin, W. H. Chen, C. N., Sheu, S. J., Huang, S. J. and Chen, Y. L. (2001). Development of Antrodia Camphorata mycelium with submerge culture. Fungal Science 16:7-22.
Chen, J. J., Lin, W. J., Liao, C. H. and Shieh, P. C. (2007). Anti-inflammatory benzenoids from Antrodia camphorate. Journal of Natural Products 70:989-992.
Clement, B. H., Eonard, M., Rissel, M., Druguent, J. A., Grimand and Herbage, K. (1984). Cellular origin of collagen and fibronectin in the liver. Cell Mol Biol 30:489-496.
Davis, T. M., Binh, T. Q., Danh, P. T., Dyer, J. R., St John, A., Garcia-Webb, P. and Anh, T. K. (1994). Serum vitamin A and E concentrations in acute falciparum malaria: modulators or markers of severity? Clin. Sci.(Lond) 87:505-511.
Fridovich, I. (1975). Superoxide dismutases. Annu Rev Biochem 44:147-159.
Fukuda, Y., Sakai, K., Matsunaga, S., Tokuda, H. and Tanaka, R. (2005). Cancer chemopreventive activity of lupane- and oleanane-type triterpenoids from the cones of Liquidamber styraciflua. Chem Biodivers 2:421-428.
Gabriele, B. (1997). Silymarin retards collagen accumulation in early and advanced biliary fibrosis secondary to complete bile duct obliteration in rats. Hepatology 26:643-649.
Global Industry Analysts. (2008). Nutraceuticals, USA:Global Industry Analysts, Inc..
Global Industry Analysts. (2010). Nutraceuticals, USA:Global Industry Analysts, Inc..
Hseu, Y. C., Chang, W. C., Hseu, Y. T., Lee, C. Y., Yech, Y. J., Chen, P. C., Chen, J. Y. and Yang, H. L. (2002). Protection of oxidative damage by aqueous extract from Antrodia camphorata mycelia in normal human erythrocytes. Life Sci 71:469-482.
Hseu, Y. C., Chen, S. C., Tsai, P. C., Chen, C. S., Lu, F. J., Chang, N. W. and Yang, H. L. (2007). Inhibition of cyclooxygenase-2 and induction of apoptosis in estrogen-nonresponsive breast cancer cells by Antrodia camphorate. Food and Chemical Toxicology 45:1107-1115.
Hseu, Y. C., Yang, H. L., Lai, Y. C., Lin, J. G., Chen, G. W. and Chang, Y. H. (2004). Induction of apoptosis by Antrodia camphorata in human premyelocytic leukemia HL-60 cells. Nutr Cancer 48:189-197.
Hsiao, G., Shen, M. I., Lin, K. H., Lan, M. H., Wu, L, Y., Chou, D. S., Lin, C. H. and Sheu, J. R. (2003). Antioxidative and hepatoprotective effects of Antrodia camphorata extract. J Agric Food Chem 51:3302-3308.
Internation Federation of Clinical Chemistry (IFCC). (1986a). J. Clin. Chem. Biochem. 24:481-495.
Internation Federation of Clinical Chemistry (IFCC). (1986b). J. Clin. Chem. Biochem. 24:497-510.
Jonker, A. M., Dijkhuis, F. W. J., Boes, A., Hardonk, M. J. and Grond, J. (1992). Immunohistochemical study of extracellular matrix in acute galactosamine hepatitis in rats. Hepatology 15:423-431.
Kierszenbaum, A. L. (2005). Histology and Cell Biology: An Introduction to Pathology. Mosby, Inc, 457-473.
Lawrence, R. A. and Burk, R. F. (1976). Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71:952-928.
Lee, M. K., Ha, N. R., Yang, H., Sung, S. H., Kim, G.. H. and Kim, Y. C. (2007). Antiproliferative activity of triterpenoids from Eclipta prostrata on hepatic stellate cells. Phytomedicine 15:775-780.
Lin, S. C., Lin, Y. H., Chen, C. F., Chung, C. Y. and Hsu, S. H. (1997). The hepatoprotective and therapeutic effects of propolis ethanol extract on chronic alcohol-induced liver injuries. Am. J. Chin. Med. 25:325-332.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951). Protein easurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
Marklund, S. and Marklund, G. (1974). Involvement of the superoxyde anion radical in the auto oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem 47:469-474.
Millward, G. H. and Jezequel, A. M. (1985). Normal histology and structure in liver and biliary disease. 2nd ed., Edited by Eright, R. and Philadelphia, G. H., 13-44.
Mizuno, T. (1995). Bioactive biobolecules of mushrooms:Food function and medicinal effect of mushroom fungi. Food Reviews 11:5-21.
Moriltz, A. (2011). The amazing liver and gallbladder flush, 64.
Nutrition Business Journal. (2013). NBJ’s supplement business report 2013. http://newhope360.com/nutrition-business-journal.
Pandey, A., Ashakumary, L. and Selvakumar, P. (1995). Copra waste- A novel substrate for solid-state fermentation. Bioresource Technology, 51:217-220.
Poulose, S. M., Harris, E. D. and Patil, B. S. (2006). Antiproliferative effects of citrus limonoids against human neuroblastoma and colonic adenocarcinoma cells. Nutr Cancer 56:103-112.
Rao, A. V. and Gurfinkel, D. M. (2000). The bioactivity of saponins: triterpenoid and steroidal glycosides. Drug Metabolism and Drug Interactions 17:211-236.
Reed, D. J., Babson, J. R., Beatty, P. W., Brodie, A. E., Ellis, W. W. and Potter, D. W. (1980). High performance liquid chromatography analysis of nanomole levels of glutathione, glutathione disulfide, and related thiols and disulfides. Anal Biochem 106:55-62.
Reitman, S. and Frankel, S. (1957). A colorimetric method for determination of serum lutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 25:56-63.
Ruwart, M. J., Wilkinson, K. F., Rush, B. D. et al. (1989). The integrated value of serum procollagen III peptide over time predicts hepatic hydroxyproline content and stainable collagen in a model of dietary cirrhosis in the rat. Hepatology 10:801-806.
Saxena, R., Zucker, S. D. and Crawford, J. M. (2003). Anatomy and Physiology of the liver. In: Zakim, D. and Boyer, T. D., editors. Hepatology: a textbook of liver disease. Philadelphia: WB Saunders, 3-30.
Shen, C., Kuo, Y., Huang, R., Lin, L., Dong, M., Chang, T. and Chou, C. (2003). Studies on the bioactive principles of Antrodia camphorate. J Chin Med 14:247-258.
Shen, Y. C., Chou, C. J., Wang, Y. H., Chen, C. F., Chou, Y. C. and Lu, M. K. (2004). Anti-inflammatory activity of the extracts from mycelia of Antrodia camphorata cultured with water-soluble fractions from five different Cinnamomum species. FEMS Microbiol Lett 231:137-143.
Shen, Y. C., Wang, Y. H., Chou, Y. C., Chen, C. F., Lin, L. C., Chang, T. T., Tien, J. H. and Chou, C. J. (2004). Evaluation of the anti-inflammatory activity of zhankuic acids isolated from the fruiting bodies of Antrodia camphorate. Planta Med 70:310-314.
Sibulesky, L. (2013). Normal liver anatomy. Clinical Liver Disease 2:S1-S3.
Song, T. Y. and Yen, G. C. (2003). Protective effects of fermented filtrate from Antrodia camphorata in submerged culture against CCl4-induced hepatic toxicity in rats. J Agric Food Chem 51:1571-1577.
Song, T. Y., Hsu, S. L. and Yen, G. C. (2005). Induction of apoptosis in human hepatoma cells by mycelia of Antrodia camphorata in submerged culture. J Ethnopharmacol 100(1-2):158-167.
Song, T. Y., Hsu, S. L., Yeh, C. T. and Yen, G. C. (2005). Mycelia from Antrodia camphorata in Submerged culture induce apoptosis of human hepatoma HepG2 cells possibly through regulation of Fas pathway. J Agric Food Chem 53:5559-5564.
SPINSscan NATURAL. (2012). 52 weeks ending December 22.
Symphony IRI GROUP. (2012). FDM market sales data for herbal supplements. 52 weeks ending December 22.
Tsai, M. C., Song, T. Y., Shih, P. H. and Yen, G. C. (2007). Antioxidant properties of water-soluble polysaccharides from Antrodia cinnamomea in submerged culture. Food Chemistry 104:1115-1122.
Tsai, Z. T. and Liaw, S. L. (1985). The used and the effect of Ganoderma. Sheng-Yun Publisher Inc, Taichung, Taiwan, 116-117.
WHO. (2002). WHO Traditional Medicine Strategy 2002-2005, Switzerland.
Wisse, E. (1970). An electron microscopic study of the fenestrated endothelial lining of rat liver sinusoids. J Ultrastruct Res 31:125-150.
Wu, H., Pan, C. L., Yao, Y. C., Chang, S. S., Li, S. L. and Wu, T. F. (2006). Proteomic analysis of the effect of Antrodia camphorate extract on human lung cancer A549 cell. Proteomics 6:826-835.
Wu, S. H., Ryvarden, L. and Chang, T. T. (1997). Antrodia camphorate (“niu-chang-chih”), new combination of a medicinal fungus in Taiwan. Botanical Bulletin of Academia Sinica 38:273-275.
Yang, L., Wu, S., Zhang, Q., Liu, F. and Wu, P. (2007). 23,24-Dihydrocucurbitacin B induces G2/M cell-cycle arrest and mitochondria-dependent apoptosis in human breast cancer cells (Bcap37). Cancer Lett 256:267-278.
Yazawa, Y., Yokota, M. and Sugiyama, K. (2000). Antitumor promoting effect of an active component of Polyporus, ergosterol and related compounds on rat urinary bladder carcinogenesis in a short-term test with concanavalin A. Biol Pharm Bull 23:1298-1302.
Zang, M. and Su, C. H. (1990). Ganoderma camphoratum, a new taxon in genus Ganoderma from Taiwan. Acta Botanica Yunnanica 12:395-396.

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top