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研究生:林思吟
研究生(外文):Lin, Sih-Yin
論文名稱:高血壓對老化之血管內皮功能和抗氧化的影響
論文名稱(外文):Effects of Hypertension on Aging-related Endothelial Function and Antioxidant Activity
指導教授:楊艾倫楊艾倫引用關係
指導教授(外文):Yang, Ai-Lun
口試日期:2017-06-09
學位類別:博士
校院名稱:臺北市立大學
系所名稱:運動科學研究所
學門:民生學門
學類:運動科技學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:中文
論文頁數:43
中文關鍵詞:老化高血壓血管功能抗氧化活性
外文關鍵詞:AgingHypertensionVascular functionAntioxidant activity
相關次數:
  • 被引用被引用:1
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  • 下載下載:22
  • 收藏至我的研究室書目清單書目收藏:0
背景及目的:目前世界人口有老年化之趨勢,在老化人口中發現因年齡的增長罹患高血壓風險增高,且心血管功能亦隨年齡逐漸下降。有許多研究討論老化與心血管功能變化之關係,但高血壓對老化心血管功能的影響不甚清楚,故本研究欲探究高血壓對老化之血管內皮功能和抗氧化活性之影響。方法:本研究使用雄性自發性高血壓大鼠(spontaneous hypertension rat, SHR),隨機分為兩組,分別為二十四週齡大的高血壓成年組和四十八週齡大的高血壓老化組,另以同週齡之雄性正常血壓大鼠(Wistar-Kyoto rat, WKY)做為對照組。各組於實驗結束時以組織浴系統評估血管內皮功能,並進行相關血清分析,包括一氧化氮(nitric oxide, NO)和抗氧化活性(過氧化氫酶catalase, CAT、超氧歧化酶superoxide dismutase, SOD)等,最後進行各組之統計和比較。結果:本研究發現血管舒張反應方面,與同週齡WKY組相較,SHR-24組和SHR-48組二組之內皮依賴性舒張反應皆顯著下降(p < 0.05),且SHR-48組與SHR-24組相較反應則顯著減少(p < 0.05),此外,SHR-24組和SHR-48組二組之非內皮依賴性舒張反應亦顯著下降(p < 0.05)。而血清中一氧化氮含量與抗氧化酵素活性(CAT、SOD)方面,SHR-24組和SHR-48組與同週齡之WKY組比較二組含量亦顯著降低(p < 0.05),其中CAT的部分,SHR-48組顯著低於SHR-24組(p < 0.05)。結論:本研究證實高血壓會造成老化之血管舒張反應更為下降,此與一氧化氮含量和抗氧化活性(CAT, SOD)降低有關。


關鍵字:老化、高血壓、血管功能、抗氧化活性
Backgroud and purpose: Aging has become a trend in the world. Advancing age is associated with the prevalence of hypertension and the decline of cardiovascular function. Many evidences have discussed about the relationships of aging and cardiovascular function, however, the effects of hypertension on aging-related cardiovascular function remained unclear. Therefore, the purpose of this study was to investigate the effects of hypertension on aging-related endothelial function and antioxidant activity. Methods: The male spontaneously hypertensive rats (SHR) were randomly divided into two groups:twenty-four-week-old adult SHR (SHR-24) and forty-eight-week-old aging SHR (SHR-48) groups, while the age-matched Wistar-Kyoto (WKY) rats were served as the normotensive control (WKY-24 and WKY-48) groups. At the end of experiments, the endothelial function was evaluated by the organ bath system among four groups. Furthermore, serum concentrations of nitric oxide (NO) and antioxidant activity (catalase, CAT; superoxide dismutase, SOD) were measured and compared among four groups. Results: We found that, compared with the age-matched WKY groups, the endothelium-dependent vasorelaxation was significantly (p < 0.05) impaired in SHR-24 and SHR-48 groups. This vasorelaxation was worse in the SHR-48 compared with the SHR-24 group (p < 0.05). Also, the endothelium-independent vasorelaxtion was significantly (p < 0.05) decreased in SHR-24 and SHR-48 groups. Moreover, serum concentrations of NO and antioxidant activity (CAT, SOD) were significantly (p < 0.05) reduced in both SHR-24 and SHR-48 groups, compared with their age-matched WKY groups. The CAT activity was significantly (p < 0.05) decreased in the SHR-48 group compared with the SHR-24 group. Conclusions: This study indicated that hypertension resulted in worse aging-related vasorelaxant responses. This could be associated with the decline of NO concentration and antioxidant activity (CAT, SOD).

Keywords:Aging, Hypertension, Vascular function, Antioxidant activity
論文授權書..................................................i
學位考試審定書..............................................ii
中文摘要..................................................iii
英文摘要....................................................v
謝誌.....................................................vii
目錄....................................................viii
表目錄......................................................x
圖目錄.....................................................xi
第壹章 緒論.................................................1
第一節 研究背景..........................................1
第二節 研究目的..........................................2
第三節 研究問題..........................................2
第四節 研究假設..........................................3
第貳章 文獻探討..............................................4
第一節 老化與高血壓.......................................4
第二節 老化、高血壓與內皮功能..............................6
第三節 老化與氧化壓力.....................................8
第四節 文獻總結..........................................9
第參章 材料與方法...........................................11
第一節 實驗動物分組......................................11
第二節 休息時心率與血壓量測...............................11
第三節 血管舒張反應量測..................................12
第四節 一氧化氮含量分析..................................14
第五節 抗氧化活性分析....................................15
第六節 統計分析.........................................16
第肆章 結果................................................17
第一節 基本生理參數......................................17
第二節 主動脈之血管舒張反應變化............................18
第三節 血清一氧化氮之含量分析.............................19
第四節 血清抗氧化活性分析.................................20
第伍章 討論與結論...........................................21
參考文獻...................................................26



表目錄

表1 二十四週齡大與四十八週齡大之正常血壓與高血壓大鼠,體重、休息時心跳
與血壓參數.............................................35



圖目錄

圖1 二十四週齡大高血壓與正常血壓大鼠,由十二至二十四週之心跳、收縮壓、
舒張壓、平均動脈壓變化曲線圖..............................36
圖2 四十八週齡大高血壓與正常血壓大鼠,由二十四週至四十八週之心跳、收縮
壓、舒張壓、平均動脈壓變化曲線圖...........................37
圖3 二十四週齡大與四十八週齡大之高血壓與正常血壓大鼠,累積劑量之乙醯膽
鹼對於胸主動脈血管所調節之血管舒張反應曲線圖.................38
圖4 二十四週齡大與四十八週齡大之高血壓與正常血壓大鼠,累積劑量之硝普鈉
對於胸主動脈血管所調節之血管舒張反應曲線圖...................39
圖5 二十四週齡大與四十八週齡大之高血壓與正常血壓大鼠,單一劑量乙醯膽鹼
對於胸主動脈血管所調節之血管舒張反應........................40
圖6 二十四週齡大與四十八週齡大之高血壓與正常血壓大鼠,一氧化氮
(Nitrate+Nitrite)含量分析..............................41
圖7 二十四週齡大與四十八週齡大之高血壓與正常血壓大鼠,過氧化氫酵素活性
變化..................................................42
圖8 二十四週齡大與四十八週齡大之高血壓與正常血壓大鼠,超氧歧化酶活性變
化....................................................43
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