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研究生:劉家良
研究生(外文):Liu, Jia-Liang
論文名稱:十二週耐力訓練對老化高血壓血管內皮功能之影響
論文名稱(外文):Effects of Twelve-weeks Endurance Training on Endothelial Function in Aging Hypertension
指導教授:楊艾倫楊艾倫引用關係
指導教授(外文):Yang, Ai-Lun
口試日期:2019-06-25
學位類別:碩士
校院名稱:臺北市立大學
系所名稱:運動科學研究所
學門:民生學門
學類:運動科技學類
論文種類:學術論文
論文出版年:2019
畢業學年度:107
語文別:中文
論文頁數:43
中文關鍵詞:運動乙醯膽鹼內皮細胞損傷血管功能
外文關鍵詞:ExerciseEndothelial damageAcetylcholineVascular function
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背景和目的:文獻指出心血管疾病風險隨年齡增長而增加,主要原因為老化造成心血管功能下降,包括動脈壓升高、血管硬化、內皮功能失能等。耐力運動已被証實有效改善心血管健康與降低心血管疾病風險,然而耐力運動對於老化高血壓之心血管功能的影響尚未釐清,故本研究欲探討長期耐力訓練對於老化高血壓之血管內皮功能的影響和機制。方法:本研究使用雄性老化自發性高血壓大鼠 (SHR),隨機分為老化高血壓運動組 (48wk-SHR-EX, n = 8)和老化高血壓控制組 (48wk-SHR, n = 8),另外再以同週齡正常血壓大鼠 (WKY)作為老化正常血壓控制組 (48wk-WKY, n = 8),共分為三組。老化高血壓運動組給予為期十二週耐力訓練,其他組別則不進行運動介入,最後進行血管內皮功能、血清一氧化氮濃度和氧化、抗氧化指標檢測,包括超氧歧化酶(superoxide dismutase, SOD)、過氧化氫酶(catalase, CAT)和脂質過氧化物(malondialdehyde, MDA),並進行三組統計分析。結果:本研究發現經十二週耐力訓練後,老化高血壓運動組之內皮依賴性血管舒張功能顯著高於其控制組 (p < 0.05),但仍低於老化正常血壓控制組 (p < 0.05);而非內皮依賴性血管舒張功能方面,老化高血壓運動組亦顯著高於其控制組 (p < 0.05)。此外,血清一氧化氮濃度方面,老化高血壓運動組顯著高於其控制組,抗氧化指標 (SOD、CAT)方面,老化高血壓運動組亦顯著高於其控制組 (p < 0.05);但脂質過氧化物含量分析則是老化高血壓運動組顯著低於其控制組 (p < 0.05)。結論:本研究證實透過長期耐力訓練,可有效改善老化高血壓大鼠血管內皮功能,並有效提升血清中一氧化氮濃度與抗氧化指標。
Background and purpose : Previous studies have demonstrated that the risk of cardiovascular disease would be increased with advanced aging. Aging causes the decreases of cardiovascular function, including arterial hypertension, vascular stiffness, and endothelial dysfunction. Endurance exercise is well known in improving cardiovascular health and lowering the risk of cardiovascular disease. However, the influences of endurance exercise on aging hypertension-related cardiovascular function have not been clarified. Therefore, this study aimed to investigate the effects of long-term endurance training on endothelial function in aging hypertension, and underlying mechanisms. Methods : Male aging spontaneously hypertensive rats (SHR) were randomly divided into exercise group (48wk-SHR-EX, n = 8) and control (48wk-SHR, n = 8) group. The age-matched Wistar-Kyoto (48wk-WKY, n = 8) rats were used as the normotensive control group. The exercise group received the endurance training for 12 weeks, otherwise, the other groups did not receive exercise intervention. Finally, the endothelial function, serum nitric oxide (NO), oxidative and antioxidant indicators were measured, including superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA). The results were compared among three groups statistically. Results : Our results showed that the endothelium-dependent vasodilation in the 48wk-SHR-EX group was significantly higher than the 48wk-SHR group (p < 0.05), but this vasodilation was significantly lower than the 48wk-WKY group (p < 0.05). The endothelium-independent vasodilation in the 48wk-SHR-EX group was significantly higher than the 48wk-SHR group (p < 0.05). In addition, the serum NO concentration in the 48wk-SHR-EX group was significantly higher than the 48wk-SHR group (p < 0.05). The antioxidant activities (SOD and CAT) in the 48wk-SHR-EX group were significantly higher than the 48wk-SHR group (p < 0.05). However, the MDA concentration in the 48wk-SHR-EX group was significantly lower than the 48wk-SHR group (p < 0.05). Conclusion : This study suggested that long-term endurance training effectively ameliorated endothelial function in aging hypertensive rats, and enhanced the NO concentration and antioxidant indicators.
中文摘要............................................... i
英文摘要............................................... iii
謝誌................................................... v
目錄................................................... vi
表目錄.................................................viii
圖目錄................................................. ix
第壹章 緒論............................................ 1
第一節 研究背景.............................. 1
第二節 研究目的................................ 2
第三節 研究問題................................ 2
第四節 研究假設................................ 2
第貳章 文獻探討........................................ 4
第一節 老化、高血壓與心血管疾病.................. 4
第二節 久坐與心血管疾病......................... 5
第三節 內皮細胞與心血管疾病之關係................ 6
第四節 耐力訓練對老化心血管功能之成效............ 7
第參章 研究方法........................................ 10
第一節 實驗設計................................ 10
第二節 安靜心率與血壓測量....................... 10
第三節 耐力運動介入方法......................... 11
第四節 血管舒張反應測量......................... 11
第五節 一氧化氮含量分析......................... 14
第六節 抗氧化活性與脂質過氧化物分析.............. 15
第七節 統計分析................................ 16
第肆章 研究結果........................................ 17
第一節 基本生理參數............................. 17
第二節 主動脈之血管舒張反應...................... 18
第三節 一氧化氮之含量分析....................... 19
第四節 抗氧化物酵素之活性分析.................... 19
第五節 脂質過氧化物脂含量分析.................... 20
第伍章 討論與結論....................................... 21
參考文獻................................................ 26

表目錄

表1四十八週齡之老化高血壓運動組 (48wk-SHR-EX)、老化高血壓控制組 (48wk-SHR) 和老化正常血壓控制組 (48wk-WKY) 之基本生理參數....34

圖目錄

圖一、 四十八週齡之老化高血壓運動組 (48wk-SHR-EX)、老化高血壓控制組 (48wk-SHR) 和老化正常血壓控制組 (48wk-WKY),累積劑量之乙醯膽鹼 (ACh)對於大鼠胸主動脈血管調節之血管舒張反應曲線圖...35

圖二、 四十八週齡之老化高血壓運動組 (48wk-SHR-EX)、老化高血壓控制組 (48wk-SHR) 和老化正常血壓控制組 (48wk-WKY),累積劑量之乙醯膽鹼 (ACh)對於大鼠胸主動脈血管去除內皮細胞調節之血管舒張反應曲線圖...................................................36

圖三、 四十八週齡之老化高血壓運動組 (48wk-SHR-EX)、老化高血壓控制組 (48wk-SHR) 和老化正常血壓控制組 (48wk-WKY),累積劑量之硝普鈉(SNP)對於大鼠胸主動脈血管調節之血管舒張反應曲線圖....37

圖四、 四十八週齡之老化高血壓運動組 (48wk-SHR-EX)、老化高血壓控制組 (48wk-SHR) 和老化正常血壓控制組 (48wk-WKY),於L-NAME (10-6M) 抑制劑作用下,單一劑量乙醯膽鹼 (ACh) 對於大鼠胸主動脈血管調節之血管舒張反應......................................38

圖五、 四十八週齡之老化高血壓運動組 (48wk-SHR-EX)、老化高血壓控制組 (48wk-SHR) 和老化正常血壓控制組 (48wk-WKY),於wortmannin (3X10-7M) 抑制劑作用下,單一劑量乙醯膽鹼 (ACh) 對於大鼠胸主動脈血管調節之血管舒張反應.........................39

圖六、 四十八週齡之老化高血壓運動組 (48wk-SHR-EX)、老化高血壓控制組 (48wk-SHR) 和老化正常血壓控制組 (48wk-WKY),一氧化氮 (NO) 之含量分析..........................................40

圖七、 四十八週齡之老化高血壓運動組 (48wk-SHR-EX)、老化高血壓控制組 (48wk-SHR) 和老化正常血壓控制組 (48wk-WKY),過氧化氫酵素 (CAT) 之活性分析......................................41

圖八、 四十八週齡之老化高血壓運動組 (48wk-SHR-EX)、老化高血壓控制組 (48wk-SHR) 和老化正常血壓控制組 (48wk-WKY),超氧歧化酶 (SOD) 之活性分析.........................................42

圖九、 四十八週齡之老化高血壓運動組 (48wk-SHR-EX)、老化高血壓控制組 (48wk-SHR) 和老化正常血壓控制組 (48wk-WKY),脂質過氧化物 (MDA) 之含量分析......................................43
Bagis, S., Tamer, L., Sahin, G., Bilgin, R., Guler, H., Ercan, B., & Erdogan, C. (2003). Free radicals and antioxidants in primary fibromyalgia: an oxidative stress disorder? Rheumatology International, 25(3), 188-190. doi:10.1007/s00296-003-0427-8
Battault, S., Singh, F., Gayrard, S., Zoll, J., Reboul, C., & Meyer, G. (2015). Endothelial function does not improve with high-intensity continuous exercise training in SHR: implications of eNOS uncoupling. Hypertension Research, 39(2), 70-78. doi:10.1038/
hr.2015.114
Berkowitz, D. E., White, R., Li, D., Minhas, K. M., Cernetich, A., Kim, S., . . . Hare, J. M. (2003). Arginase Reciprocally Regulates Nitric Oxide Synthase Activity and Contributes to Endothelial Dysfunction in Aging Blood Vessels. Circulation, 108(16), 2000-2006. doi:10.1161/01.cir.0000092948.04444.c7
Biswas, A., Oh, P. I., Faulkner, G. E., Bajaj, R. R., Silver, M. A., Mitchell, M. S., & Alter, D. A. (2015). Sedentary Time and Its Association With Risk for Disease Incidence, Mortality, and Hospitalization in Adults. Annals of Internal Medicine, 162(2), 123. doi:10.7326/m14-1651
Buford, T. W. (2016). Hypertension and aging. Ageing Research Reviews, 26, 96-111. doi:10.1016/j.arr.2016.01.007
Cai, H., & Harrison, D. G. (2000). Endothelial dysfunction in cardiovascular diseases: the role of oxidant stress. Circulation Research, 87(10), 840-844.
Collins, J. A., Munoz, J.-V., Patel, T. R., Loukas, M., & Tubbs, R. S. (2014). The anatomy of the aging aorta. Clinical Anatomy, 27(3), 463-466. doi:10.1002/ca.22384
Costantino, S., Paneni, F., & Cosentino, F. (2016). Ageing, metabolism and cardiovascular disease. The Journal of Physiology, 594(8), 2061-2073. doi:10.1113/jp270538
Després, J.-P. (2016). Physical Activity, Sedentary Behaviours, and Cardiovascular Health: When Will Cardiorespiratory Fitness Become a Vital Sign? Canadian Journal of Cardiology, 32(4), 505-513. doi:10.1016/j.cjca.2015.12.006
Dogra, S., & Stathokostas, L. (2012). Sedentary Behavior and Physical Activity Are Independent Predictors of Successful Aging in Middle-Aged and Older Adults. Journal of Aging Research, 2012, 1-8. doi:10.1155/2012/190654
Endes, S. (2016). Physical activity reduces cardiovascular disease risk in older adults. Evidence Based Medicine, 21(5), 191-191. doi:10.1136/
ebmed-2016-110496
Evenson, K. R., Wen, F., & Herring, A. H. (2016). Associations of Accelerometry-Assessed and Self-Reported Physical Activity and Sedentary Behavior With All-Cause and Cardiovascular Mortality Among US Adults. American Journal of Epidemiology, 184(9), 621-632. doi:10.1093/aje/kww070
Gibbs, B. B., Hergenroeder, A. L., Katzmarzyk, P. T., Lee, I. M., & Jakicic, J. M. (2015). Definition, Measurement, and Health Risks Associated with Sedentary Behavior. Medicine & Science in Sports & Exercise, 47(6), 1295-1300. doi:10.1249/mss.0000000000000517
Jakovljevic, B., Nikolic Turnic, T., Jeremic, N., Jeremic, J., Bradic, J., Ravic, M., . . . Zivkovic, V. (2018). The impact of aerobic and anaerobic training regimes on blood pressure in normotensive and hypertensive rats: focus on redox changes. Molecular and Cellular Biochemistry, 454(1-2), 111-121. doi:10.1007/s11010-018-3457-y
Klöß, S., Bouloumié, A., & Mülsch, A. (2000). Aging and chronic hypertension decrease expression of rat aortic soluble guanylyl cyclase. Hypertension, 35(1), 43-47.
Kumral, Z., Sener, G., Ozgur, S., Koc, M., Suleymanoglu, S., Hurdag, C., & Yegen, B. (2016). Regular exercise alleviates renovascular hypertension-induced cardiac/endothelial dysfunction and oxidative injury in rats. Journal of Physiology and Pharmacology, 67(1), 45-55.
Masodsai, K. (2017). Exercise and Endothelial Dysfunction in Hypertension. Adaptive Medicine, 8(1), 1-14. doi:10.4247/am.
2017.abg168
Messner, B., & Bernhard, D. (2014). Smoking and Cardiovascular Disease: Mechanisms of Endothelial Dysfunction and Early Atherogenesis. Arteriosclerosis, Thrombosis, and Vascular Biology, 34(3), 509-515. doi:10.1161/atvbaha.113.300156
Pal, S., Radavelli-Bagatini, S., & Ho, S. (2013). Potential benefits of exercise on blood pressure and vascular function. Journal of the American Society of Hypertension, 7(6), 494-506. doi:10.1016/
j.jash.2013.07.004
Pescatello, L. S., Franklin, B. A., Fagard, R., Farquhar, W. B., Kelley, G. A., & Ray, C. A. (2004). Exercise and Hypertension. Medicine & Science in Sports & Exercise, 36(3), 533-553. doi:10.1249/
01.mss.0000115224.88514.3a
Qiu, F., Liu, X., Zhang, Y., Wu, Y., Xiao, D., & Shi, L. (2018). Aerobic exercise enhanced endothelium-dependent vasorelaxation in mesenteric arteries in spontaneously hypertensive rats: the role of melatonin. Hypertension Research, 41(9), 718-729. doi:10.1038/s41440-018-0066-9
Roberts, D. D., Villalpando, D. M., Navarro, R., del Campo, L., Largo, C., Muñoz, D., . . . Ferrer, M. (2017). Docosahexaenoic Acid Supplemented Diet Influences the Orchidectomy-Induced Vascular Dysfunction in Rat Mesenteric Arteries. Plos One, 12(1), e0168841. doi:10.1371/journal.pone.0168841
Romero, S. A., Minson, C. T., & Halliwill, J. R. (2017). The cardiovascular system after exercise. Journal of Applied Physiology, 122(4), 925-932. doi:10.1152/japplphysiol.00802.2016
Schreuder, T. H. A., Maessen, M. F. H., Tack, C. J., Thijssen, D. H. J., & Hopman, M. T. E. (2013). Life-long physical activity restores metabolic and cardiovascular function in type 2 diabetes. European Journal of Applied Physiology, 114(3), 619-627. doi:10.1007/
s00421-013-2794-5
Seguí Díaz, M. (2015). Sedentarismo y mortalidad. SEMERGEN - Medicina de Familia, 41(8), 450-451. doi:10.1016/j.semerg.
2015.03.002
Sehgel, N. L., Sun, Z., Hong, Z., Hunter, W. C., Hill, M. A., Vatner, D. E., . . . Meininger, G. A. (2014). Augmented Vascular Smooth Muscle Cell Stiffness and Adhesion When Hypertension Is Superimposed on Aging. Hypertension, 65(2), 370-377. doi:10.1161/
hypertensionaha.114.04456
Sekiguchi, F., Miyake, Y., Hirakawa, A., Nakahira, T., Yamaoka, M., Shimamura, K., . . . Sunano, S. (2001). Hypertension and impairment of endothelium-dependent relaxation of arteries from spontaneously hypertensive and L-NAME-treated Wistar rats. Journal of Smooth Muscle Research, 37(2), 67-79.
Sharman, J. E., La Gerche, A., & Coombes, J. S. (2014). Exercise and Cardiovascular Risk in Patients With Hypertension. American Journal of Hypertension, 28(2), 147-158. doi:10.1093/ajh/hpu191
Shortreed, S. M., Peeters, A., & Forbes, A. B. (2013). Estimating the effect of long-term physical activity on cardiovascular disease and mortality: evidence from the Framingham Heart Study. Heart, 99(9), 649-654. doi:10.1136/heartjnl-2012-303461
Siasos, G., Athanasiou, D., Terzis, G., Stasinaki, A., Oikonomou, E., Tsitkanou, S., . . . Tousoulis, D. (2016). Acute effects of different types of aerobic exercise on endothelial function and arterial stiffness. European Journal of Preventive Cardiology, 23(14), 1565-1572. doi:10.1177/2047487316647185
Tanaka, H. (2017). Aging of Competitive Athletes. Gerontology, 63(5), 488-494. doi:10.1159/000477722
Tanaka, L. Y., Bechara, L. R. G., dos Santos, A. M., Jordão, C. P., de Sousa, L. G. O., Bartholomeu, T., . . . Ramires, P. R. (2015). Exercise improves endothelial function: A local analysis of production of nitric oxide and reactive oxygen species. Nitric Oxide, 45, 7-14. doi:10.1016/j.niox.2015.01.003
Tian, X.-L., & Li, Y. (2014). Endothelial Cell Senescence and Age-Related Vascular Diseases. Journal of Genetics and Genomics, 41(9), 485-495. doi:10.1016/j.jgg.2014.08.001
van Gorp, A. W., Schenau, D. S. V. I., Hoeks, A. P., Boudier, H. A. S., de Mey, J. G., & Reneman, R. S. (2000). In spontaneously hypertensive rats alterations in aortic wall properties precede development of hypertension. American Journal of Physiology-Heart and Circulatory Physiology, 278(4), H1241-H1247.
Venturelli, M., Cè, E., Limonta, E., Schena, F., Caimi, B., Carugo, S., . . . Esposito, F. (2015). Effects of endurance, circuit, and relaxing training on cardiovascular risk factors in hypertensive elderly patients. Age, 37(5). doi:10.1007/s11357-015-9835-4
Veskoukis, A. S., Paschalis, V., Kyparos, A., & Nikolaidis, M. G. (2018). Administration of exercise-conditioned plasma alters muscle catalase kinetics in rat: An argument for in vivo -like K m instead of in vitro -like V max. Redox Biology, 15, 375-379. doi:10.1016/j.redox.
2018.01.001
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