跳到主要內容

臺灣博碩士論文加值系統

(216.73.217.60) 您好!臺灣時間:2026/06/17 08:55
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:林郁涵
研究生(外文):Yu-Han Lin
論文名稱:硫代硫酸銀及硫酸鋁對延長玫瑰與洋桔梗切花保鮮及玫瑰切花利用硫代硫酸銀處理與花瓣端節酵素活性變化之研究
論文名稱(外文):Studies on the postharvest life of cut rose and eustoma flowers as affected by silver thiosulfate and aluminum sulfate and the telomerase activity in silver thiosulfate treated cut rose flowers
指導教授:陳文孝廖麗貞
指導教授(外文):Wen-Shaw ChenLi-Jen Liao
學位類別:碩士
校院名稱:國立高雄師範大學
系所名稱:生物科學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2001
畢業學年度:89
語文別:中文
論文頁數:36
中文關鍵詞:切花保鮮玫瑰切花洋桔梗切花保鮮劑端節酵素
外文關鍵詞:postharvest lifecut rose flowercut eustoma flowerpreservativetelomerase
相關次數:
  • 被引用被引用:1
  • 點閱點閱:1126
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
本論文分為兩部分,第一部份研究硫代硫酸銀(silver thiosulfate)及蔗糖對玫瑰切花之瓶插壽命的影響。第二部分研究硫酸鋁(aluminum sulfate)對洋桔梗切花之瓶插壽命的影響。
在玫瑰(Rosa hybrida L. cv. Diana)切花方面,在23℃下,以濃度為0,20,40,60,80,100,120 gL-1的蔗糖溶液,其中均含200 mgL-1 8-hydroxyquinoline sulfate(8-HQS)處理10小時後,轉移至蒸餾水中繼續瓶插,結果發現每公升含120克蔗糖之溶液可使玫瑰切花維持最長的壽命約7天。若將切花瓶插於0.2 mM 硫代硫酸銀溶液中2小時後,再換到含120 gL-1蔗糖及200 mgL-1 8-HQS的溶液中10小時,最後換到蒸餾水中繼續瓶插,則切花瓶插壽命約10天且花朵品質最佳。瓶插於0.2 mM 硫代硫酸銀溶液中2小時後,直接換到蒸餾水中繼續瓶插,其切花瓶插壽命約9天。剛採收的切花之乙烯生成量為最高,經過不同化學藥劑瓶插處理後,乙烯生成量均有下降的現象。與對照組相比,蔗糖抑制乙烯生成的效果為佳,其次為硫代硫酸銀處理後換以蔗糖處理組,單獨以硫代硫酸銀處理之效果並不顯著。另外,就端節酵素活性表現可得知,其活性高低與保鮮劑效果呈現相關。
在洋桔梗(Eustoma grandiflorum Shinn. cv. Hei Hou)切花方面,在25℃下,以濃度為0,50,100,150 mgL-1的硫酸鋁溶液進行瓶插處理,發現150 mgL-1硫酸鋁溶液處理後可獲得最長的瓶插壽命約15天,其次依序為100 mgL-1硫酸鋁、50 mgL-1硫酸鋁、蒸餾水處理。在水分散失量上,硫酸鋁處理組比水處理組散失較少量的水,亦即水分散失量被硫酸鋁所抑制,因此硫酸鋁處理組之切花可吸收較多的水分,故鮮重較水處理組高。實驗結果得知,硫酸鋁為一有效的殺菌劑,減少切花發生維管束阻塞的情形,而延長其瓶插壽命。
Cut rose(Rosa hybrida L. cv. Diana)flowers were kept at 23℃ in solution containing 0, 20, 40, 60, 80, 100, 120gL-1 sucrose in combination with 200 mgL-1 8-hydroxyquinoline sulfate(8-HQS)for 10 h, then transferred to distilled water. The pulse treatment of sucrose at 120gL-1 had a significant effect on extending the vase life of cut rose flowers about 7 days. The pulse treatment of silver thiosulfate(STS)0.2mM for 2 h followed by sucrose at 120gL-1 with 200 mgL-1 8-hydroxyquinoline sulfate(8-HQS)for 10 h had the longest vase life(about 10 days)and best flower quality. Flowers with only silver thiosulfate(STS)0.2mM for 2 h maintained vase life for 9 days. The ethylene production was greatest in untreated rose flower and decreased by applying chemical solutions treatment. Comparing with water control, ethylene production was more significantly inhibited by sucrose in combination with 8-HQS than with STS or STS followed by sucrose along with 8-HQS. And the telomerase activity is related to the use of preservatives. Cut rose flowers applying STS followed by sucrose show the positive expression of telomerase activity.
Cut eustoma(Eustoma grandiflorum Shinn. cv. Hei Hou)flowers were placed in a preservative solution containing 0, 50, 100, 150mgL-1 aluminum sulfate at 25℃. We found that the vase life of cut eustoma flower was significantly extended when using 150mgL-1 of aluminum sulfate to 15 days, whereas the vase life of the water control was only 8 days. Cut flowers kept in 150mgL-1 of aluminum sulfate lost less water than those in distilled water. The results suggest that transpiration was inhibited by aluminum sulfate and also indicate that cut flower treated with aluminum sulfate absorbed more water than those in water control. This allowed more increase in fresh weight of cut eustoma flower in 150mgL-1 of aluminum sulfate. So aluminum sulfate is an effective antimicrobial agent to prevent blockage of vascular bundles for extending vase life.
誌謝…………………………………………………………...Ⅰ
中文摘要……………………………………………………...Ⅱ
英文摘要……………………………………………………...Ⅳ
目錄…………………………………………………………...Ⅵ
圖表目錄……………………………………………………...Ⅶ
前言…………………………………………………………….1
第一部份:硫代硫酸銀及蔗糖對玫瑰切花瓶插壽命及端節酵素活性之影響………………………………………………….4
研究背景…………………………………………………….4
材料與方法………………………………………………….7
結果與討論………………………………………………...12
第二部分:硫酸鋁對洋桔梗切花之瓶插壽命影響………...21
研究背景…………………………………………………...21
材料與方法………………………………………………...22
結果與討論………………………………………………...23
參考文獻……………………………………………………...31
黃肇家。1997。切花保鮮要點。高雄區農業專訊19: 7-9。
王自存。1995。切花的保鮮。園產品處理學實習講義.19-21。
高景輝。1994。植物荷爾蒙。華香園。
高景輝。1987。植物生長與分化。國立編譯館。
康有德等。1997。園藝概論。國立編譯館。
Aarts, J. F. T. 1957. On the keepability of cut flowers. Meded. Landbouwhogesch. Wageningen 57: 1- 62.
Baker, J. E., Wang, C. Y., Lieberman, M. and Hardenburg, R. 1977. Delay of senescence in carnations by a rhizobitoxine analog and sodium benzoate. HortScience 12: 38-39.
Broun, R. and Mayak, S. 1981. Aminooxyacetic acid as aninhibitors of ethylene synthesis and senescence in carnation flowers. Sci. Hort. 22: 173-180.
Chin, C. K. and Sacalis, J. N. 1997. Metabolism of sucrose in cut roses.Ⅱ.Movement and inversion of sucrose absorbed by cut rose stems. J. Amer. Soc. Hort. Sci. 102:537-540.
Chung, B. C., Lee, S. Y., Oh, S. A., Rhew, T. H., Nam, H. G., and Lee, C. H. 1997. The promoter activity of sen 1, a senescence-associated gene of Arabidopsis, is repressed by sugars. J. Plant Physiol. 151: 339-345.
De Stigter, H.C.M. 1981. Effects of glucose with 8-hydroxyquinoline sulfate or aluminum sulfate on the water balance of cut Sonia roses. Z. Pflanzenphysiol. 101: 95-105.
Dansereau, B. and Vines, H. M. 1975. In-stem movement, isolation and identification of two bacteria and their antibiotic sensitivity. Acta Hort. 41:183-198.
Dimalla, G. G. and Van Staden, J. 1980. The effect of silver thiosulfate preservative on the physiology of cut carnations. 1. Influence on longevity and carbonhydrate status. Z. Pflanzenphysiol. 99: 9-17.
Durkin, D. J. 1979. Effect of millipore filtration, citric acid, and sucrose on peduncle water potential of cut rose flower. J. Amer. Soc. Hort. Sci. 104: 860-863.
Eapen, S. and George, L. 1997. Plant regeneration from peduncle segments of oil seed Brassica species: Influence of silver nitrate and silver thiosulfate. Plant Cell Tissue Organ Cult. 51: 229-232.
Fujino, D. W., Reid, M. S. and Vandermolen, G. E. 1983. Identification of vascular blockage of cut maidenhair(Adiantum raddianum)fronds. Scientia Hort. 21: 381-388.
Han, S.S. 1998. Posthartvest handling of cut Heuchera sanguinea Engelm. flowers: Effects of sucrose and silver thiosulfate. HortScience 33: 731-733.
Halevy, A. H. and Mayak, S. 1981 . Senescence and postharvest physiology of cut flowers-Part 2. Hort. Rev . 3: 59-143.
Ichimura, K. and Hiraya, T. 1999. Effect of silver thiosulfate complex(STS)in combination with sucrose on the vase life of cut sweet pea flowers. J. Japan. Soc. Hort. Sci. 68: 23-27.
Ichimura, K. and Hisamatsu, T. 1999. Effects on continuous treatments with sucrose on the vase life, soluble carbohydrate concentrations, and ethylene production of cut snapdragon flowers. J. Japan. Soc. Hort. Sci. 68: 61-66.
Ishihara, Y., Ohkawa, K. and Hyodo, H. 1991. Senescence of cut sweet pea flowers and ethylene production. J. Japan. Soc. Hort. Sci. 60: 141-147.
Jacobs, T. Control of the cell cycle. Dev. Biol. 1992, 153: 1-15.
Jones, R.B. and Hill, M. 1993. The effect of germicides on the longevity of cut flowers. J. Amer. Soc. Hort. Sci. 118: 350-354.
Kaltaler, R.E.L. and Steponkus, Peter L. 1976. Factor Affecting Respiration in Cut Roses.J. Amer. Soc. Hort. Sci. 101(4):352-354.
Kazuo Ichimura and Masaru Korenaga. 1998. Improvement of vase life and petal color expression in several cultivars of cut Eustoma flowers using sucrose with 8-hydroxyquinoline sulfate. Bull. Natl. Res. Veg., Ornam. Plants&Tea, Japan 13: 31~39.
Kazuo Ichimura, Shigefumi Ueyama and Rie Goto. 1999. Possible roles of soluble carbohydrate constituents in cut rose flowers. J. Japan. Soc. Hort. Sci. 68(3):534-539.
Kazuo Ichimura, Yuji Mukasa, Takahiro Fujiwara, Katsunori Kohata and Kenichi Suto. 1998. Improvement of postharvest life and changes in sugar concentrations by sucrose treatment in bud-cut sweet pea. Bull. Natl. Res. Veg., Ornam. Plants&Tea, Japan 13: 41~49.
Kazuo Ichimura and Shigefumi Ueyama. 1998. Effects of temperature and application of aluminum sulfate on the postharvest life of cut rose flowers. Bull. Natl. Res. Veg., Ornam. Plants&Tea, Japan 13: 51~60.
Kilian, A., Stiff, C. & Kleinhofs, A. Barley telomeres shorten during differentiation but grow in callus culture. Proc. Natl. Acad. Sci. USA 1995, 92: 9555-9559.
Lincoln Taiz, Eduardo Zeiger. 1991. Plant physiology. The Benjamin/Cummings Publishing Company, Inc.
Matthew S. Fitzgerald, Thomas D. Mcknight, and Dorothy E. Shippen. 1996. Characterization and developmental patterns of telomerase expression in plants. Proc. Natl. Acad. Sci. USA. Vol. 93, pp. 14422-14427.
Mayak, S., Halevy, A. H., Sagie, S., Bar-Yoseph, A., and Bravdo, B. 1974. The water balance of cut rose flowers.Physiol. Plant. 31: 15-22.
Mayak, S., Garibaldi, E. A. and Kofranek, A. M. 1977. Carnation flower longevity: Microbial populations as related to silver nitrate stem impregnation. J. Amer. Soc. Hort. Sci. 102: 637-639.
Meir, S., Philosoph-Hadas, S., Michaeli, R. and H. Davidson. 1995. Improvement of the keeping quality of mini-gladiolus spikes during prolonged storage by sucrose pulsing and modified atmosphere packing. Acta Hort. 405: 335-342.
Midoh, N., Saijou, Y., Matsumoto, K. and Iwata, M. 1996. Effects of 1,1-dimethy1-4-(phenylsulfonyl) semicarbavide (DPSS) on carnation flower longevity. Plant Growth Regulation 20: 195-199.
Mor, Y., Reid, M. S. and Kofranek, A. M. 1984. Pulse treatments with silver sulfate and sucrose improve the vase life of sweet peas. J. Amer. Soc. Hort. Sci 109: 866-868.
Nakai, T., Tonouchi, N., Tsuchida, T., Mori, H., Sakai, F. and Hayishi, T. 1997. Synthesis of asymmetrically labeled sucrose by a recombinant sucrose synthase. Bioscience Biotech. Biochem. 61: 1955-1956.
Paulin, A. and Jamain, C. 1982. Development of flowers and changes in various sugars during opening of cut carnations. J. Amer. Soc. Hort. Sci. 107: 258-261.
Sacalis, J. N. and Chin, C. K. 1976. Metabolism of sucrose in cut roses. I. Comparison of sucrose pulse and continuous sucrose uptake. J. Amer. Soc. Hort. Sci. 101: 254-257.
Sarkar, D., Kaushik, S. K. and Naik, P. S. 1999. Minimal growth conservation of potato microplants: Silver thiosulfate reduces ethylene-induced growth abnormalities during prolonged storage in vitro. Plant Cell Rep. 18: 897-903.
Sexton, R., Porter, A. E., Littlejohns, S. and Thain, S. C. 1995. Effects of diazocyclopentadiene(DACP)and silver thiosulfate(STS)on ethylene regulated abscission of sweet pea flowers(Lathyrus odoratus L.). Ann. Bot. 75: 337-342.
Serrano, M., Romojaro, F., Casas, J. L., Del Rio, J. A. and Acosta, M. 1990. Action and mechanism of a —aminoiso-butyric acid as a retardant of cut carnation senescence.Sci. Hort. 44: 127-134.
Sexton, R., Porter, A. E., Littlejohns, S. and Thain, S. C. 1995. Effects of diazocyclopentadiene (DACP) and silver thiosulfate (STS) on ethylene regulated abscission of sweet pea flowers (Lathyyus odoratus L). Ann. Bot. 75; 337-342.
Stead, A. D. and Moore, K. G. 1983. Studies on flower longevity in Digitalis. The role of ethylene in corolla abscission. Planta 157: 15-21.
Sissel Torre, Amihud Borochov and Abraham H. Halevy. 1999. Calcium Regulation of Senescence in Rose Petals. Physiologia Plantarum 107: 214-219.
Uda, A., Yamanaka, M. and Fukushima, K. 1997. Pretreatment effect of novel ethylene inhibitors on extending longevity of carnation, larkspur and sweet pea cut flowers. Kinki chugoku Agr. Res. 93: 65-70 (In Japanese with English summary ).
Van Doorn, W. G., Groenewegen, G., Van de Pol, P. A. and Berkholst, C. E. M. 1991. Effects of carbohydrate and water status on flower opening of cut Madelon roses. Postharv. Biol. Techno. 1: 47-57.
Van Doorn, W.G. and Reid, M. S. 1992. Role of ethylene inflower senescence of Gypsophila paniculata L. Postharvest Biol. Technol. 1: 265-272.
Van Doorn, W.G., De Witte, Y., and Perik, R. R. J. 1990. Effect of antimicrobial compounds on the number of bacteria in stems of cut rose flowers. J. Appl. Bacteriol. 68: 117-122.
Van Doorn, W.G., De Witte, Y., and Perik, R. R. J. 1990. Hydroxyquinoline citrate and low pH prevent vascular blockage in stem of cut rose flowers by reducing the number of bacteria. J. Amer. Soc. Hort. Sci. 115: 979-981.
Van Doorn, W. G. 1997. Water relation of cut flowers. Hort. Rev. 18, 1-85.
Yakimova, E., Kapchina, T. V., Alexieva, V., Sergiev, I. and Karanov, E. 1996. Effect of chlorsulfuron(Glean-75)and sucrose on some post-harvest physiological events in cut flowers. Bulgarian J. Plant physiol. 22: 74-87.
Yu, S.J., Kim, S., Lee, J. S. and Lee, D. H. 1998. Differential accumulation of transcripts for ACC synthase and ACC oxidase homologs in etiolated mung bean hypocotyls in response to various stimuli. Mol. Cell. 8: 350-358.
Zagory, D. and Reid, M. S. 1986. Role of vase solution microorganisms in the life of cut flowers. J. Amer. Soc. Hort. Sci. 111: 154-158.
Zieslin, N., Kohl Jr, H. C., Kofranek, M. C. and Halevy, A. H. 1978. Changes in the water status of cut roses and its relationship to bent-neck phenomenon. J. Amer. Soc. Hort. Sci. 103: 176-179.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top