臺灣博碩士論文加值系統

幾丁聚醣(chitosan)是一種具有生物分解性和抑菌性的高分子，不織布(non-woven)使用則是因其具有多孔性及高表面積。本研究主要是將幾丁聚醣不織布改質含浸奈米金顆粒，評估此敷料應用於醫療創傷使用的可行性。期待材料不但具有良好的生物適應性外，更希望可以協助皮膚組織的修復與癒合，避免疤痕形成及傷口收縮。材料特性分析方面，首先將幾丁聚醣(chitosan)含浸不同添加量的奈米金，進行最適化添加量的評估，得到細胞在添加40 ppm奈米金時生長最佳。但幾丁聚醣不織布表面疏水性是應用上的一個限制，所以我們使用嫘縈(rayon, R)與幾丁質(chitin, C)混合來改善，比例分別為1：1及1：3，發現混合後的材料具有良好的生物相容性，對幾丁聚醣的吸水性有改善，然降解率並無明顯變化。在動物實驗中，發現覆蓋添加奈米金幾丁聚醣敷料的傷口修復能力最優，組織學結果也證實經奈米金改質的材料，可加速傷口修復的過程，促進上皮化的現象。從細胞學與動物試驗結果證實，此項改質確有促進細胞生長與傷口修復的效果。

Chitosan is a biodegradable and antibacterial polymer. Non-woven has been used due to its porosity, allowing ventilation and high surface area. In this study, chitosan non-woven fabric was modified by infiltration with gold nanoparticles. The feasibility of applying such samples to wound dressing was evaluated. This dressing hopefully will have good biocompatibility, improve skin repair, and prevent the wound healing from complication of scar formation and wound contract.
Chitosan incorporated with various concentrations of gold (Au) nanoparticles was studied. The optimal concentration was determined. It was found that chitosan containing 40 ppm Au showed the best cell growth. Since the hydrophobic surface of chitosan non-woven had limited its application, rayon complexes with chitin was also tested. The component ratio of the materials was 1:1 or 1:3 chitin (C)-rayon (R). These blends were found to possess good biocompatibility and water uptake ability.
Chitosan non-woven containing Au nanoparticles also demonstrated an improving effect on wound healing and tissue repair in the animal model. Histological examination showed an advanced epithelialization in rat skin wounds treated with chitosan-Au sponges. In conclusion, chitosan containing Au could enhance cell proliferation and wound healing.

中文摘要 i
英文摘要 ii
目錄 iii
圖目錄 vi
表目錄 viii
簡號代稱說明 ix

第一章 緒論 ..1
1-1 研究動機及目的 ..1
1-2 生醫材料的條件評估與應用 ..3
1-3 敷料的簡介 ..5
1-4 材料選擇及加工方法 ..7
1-4-1 幾丁質與幾丁聚醣 ..8
1-4-2 嫘縈 ..10
1-5 奈米技術 ..12
1-5-1 奈米生醫材料 ..13
1-5-2 奈米金 ..15
1-6 皮膚系統 ..17
1-6-1 傷口癒合的機制 ..19
第二章 實驗材料與方法 ..22
2-1 實驗流程 ..22
2-2 樣品製備 ..23
2-3 體外試驗 ..24
2-3-1 細胞生長與貼附測試 ..24
2-3-2 幾丁聚醣敷料膨潤測試 ..26
2-3-3 生物降解性測試 ..26
2-3-4 材料毒性測試 ..27
2-4 動物實驗與組織學評估 ..28
2-4-1 術前準備 ..28
2-4-2 材料製備 ..28
2-4-3 動物手術實驗 ..28
2-4-4 臨床傷口評估 ..29
2-4-5 組織適應性評估 ..29
2-6 統計分析 ..30
第三章 實驗結果與討論 ..31
3-1 體外培養評估 ..31
3-1-1 2D膜上細胞貼附測試 ..31
3-1-2 複合材料細胞相容性測試 ..31
3-1-3 材料選擇與評估 ..32
3-1-4 膨潤度測定結果 ..32
3-1-5 不織布複合材料降解速率測定結果 ..33
3-1-6 細胞毒性測試 ..33
3-2 動物實驗觀察 ..34
3-2-1 材料支架組織適應性評估 ..34
3-2-2 不織布材料組織適應性評估 ..36
3-3 討論 ..37
第四章 結論 ..40
第五章 參考文獻 ..41

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