臺灣博碩士論文加值系統

精油為植物提煉之芳香物質，具抗菌及抗感染等功效，且精油具脂溶性、分子小與可穿透皮膚及黏膜等特性，己知植物精油大多具抗真菌、細菌等效用，廣泛應用於化妝品工業與調香業。近年來精油的生理活性逐漸受到重視，除將精油應用於傳統芳香療法外，更延伸應用於化妝品、香水及食品等等。許多學者相繼探討植物精油之抗氧化性，研究指出，過量自由基產生會引發持續性發炎反應，故抗氧化與發炎反應發展具密切相關，但相關十種市售精油抗氧化及抗發炎部分則尚未被探討。
本研究利用體外試驗探討佛陀木、德國洋甘菊與藍絲柏三種精油之抗氧化活性並利用小鼠巨噬細胞（RAW264.7cells)模式評估其抗發炎之功效。結果顯示，三種植物精油皆具有良好的還原力並能清除2.2-azino -bis (3-ethylbenzthiazoline-6-sulPphonic acid ）自由基；另一方面，在細胞試驗中顯示三種植物精油能顯著降低RAW264.7cells經脂多醣（lipopolysaccharide, LPS）所誘發一氧化氮（nitricoxide, NO）之生成。
綜上所述，佛陀木、德國洋甘菊與藍絲柏三種精油具有良好之抗氧化性並能有效降低發炎反應所產生一氧化氮，然其相關分子反應機制則有待進一步探討。

Essential oils, the aromatic substances extracted from the plants, possess anti-bacterial and anti-infection effects and easily penetrate the skin and mucous membranes. Essential oils with anti-fungal and anti-bacterial effects are wildely used in the cosmetic and perfumery industy. Recently the physiological activities of essential oils have become more important. In addition to traditional aromatherapy, the essential oils are used in cosmetics, perfumes and foods. The antioxidant activity of essential oils was investigated in many studies. It is suggested that excess free radicals may induce persistent inflammation and thus the anti-oxidant effects may be involved inassociated with the anti-inflammatory activity. However, the anti-oxidant and anti-inflammatory activities of ten commercially available selected commercial essential oils have not yet been explored.
In this study, the antioxidant activities of Eremophila mitchellii, Matricaria recutica, Callitris intratropica essential oils were studied in vitro. The mouse macrophage cells (RAW264.7cells) were used to investigate the anti-inflammatory effect of essential oils. The results showed that three essential oils possessed reducing power and significantly scavenged 2.2-azino-bis (3-ethylbenzthiazoline-6-sulPhonic acid) radicals. Moreover, three essential oils significantly reduced nitric oxide (NO) production in RAW264.7 cells stimulated by LPS (lipopolysaccharide).
In conclusion, Eremophila mitchellii, Matricaria recutica, Callitris intratropica essential oils have anti-oxidant activities and effectively reduce the production of nitric oxide in the LPS-stimulated macrophage cells. However, the molecular mechanisms need to be explored in the future.

目 錄 I
圖目錄 IV
附 件 VI
中文摘要 VII英文摘要 IX

第一章 前言1
第二章 文獻探討3
第一節 精油之簡介3
一、植物精油 3
二、精油生理活性 5
三、本研究精油植物介紹13
第二節 氧化壓力與抗氧化 22
一、自由基與活性氧 22
二、抗氧化防禦系統 27
第三節 發炎反應 34
一、LPS誘發發炎反應機制 35
二、發炎相關介質 37
三、發炎與自由基 43
第三章 研究目的與架構46
第一節 研究目的46
第二節 研究架構47
第四章 材料與方法 48
第一節 儀器設備48
第二節 藥品49
第三節 實驗方法51
一、GC-MS分析 51
二、體外抗氧化試驗 52
(一)清除ABTS自由基試驗 52
(二)還原力試驗 54
三、細胞試驗 55
(一)細胞培養試驗 55
(二)細胞毒性試驗 57
四、統計方法 59
第五章 結果60
第一節 三種精油抗氧化能力評估61
一、ABTS自由基清除能力61
二、還原力分析 63
第二節 三種精油對小鼠巨噬細胞之抗發炎活性探討64
一、三種精油對細胞存活率的影響 64
二、三種精油對LPS誘導小鼠巨噬細胞NO釋放量的影響 67
第六章 討論71
第七章 結論76
第八章 參考文獻78
附 件 103

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