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研究生:方韶瓏
研究生(外文):Chao-Lung Fang
論文名稱:在老鼠模式中急性酒精暴露對脾臟自然殺手細胞的影響
論文名稱(外文):Effect of ethanol on splenic natural killer cells in a mouse model for binge drinking
指導教授:吳文俊吳文俊引用關係
指導教授(外文):Wen-Jun Wu, Ph. D.
學位類別:碩士
校院名稱:中山醫學院
系所名稱:毒理學研究所
學門:醫藥衛生學門
學類:其他醫藥衛生學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:78
中文關鍵詞:急性酒精自然殺手細胞
外文關鍵詞:binge drinkingnatural killer cellsethanol
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  • 下載下載:37
  • 收藏至我的研究室書目清單書目收藏:1
先前已有報導指出酒精具有抑制免疫反應的作用,因而顯著增加人類對於感染疾病及癌症的發生率。酒精在免疫系統的慢性影響已被廣泛的研究,但卻少有研究在急性酒精曝露的影響上,而且急性飲酒的習慣比慢性酒精中毒者更為普遍。對急性酒精中毒或是慢性酒精中毒者來說,酒精會損害先天性及後天性免疫反應,其中自然殺手細胞是人類先天性免疫系統中最重要的作用細胞。本研究以B6C3F1母鼠為實驗動物, 32 %(6.0 g/kg)的酒精劑量為實驗劑量,測試在急性酒精曝露下,對脾臟自然殺手細胞數目的影響。結果發現酒精會脾臟誘導自然殺手細胞的凋亡因而造成自然殺手細胞數目的降低,並不是因為酒精誘導分泌的糖皮質素所造成自然殺手細胞的重新分配於各組織中。因為各組織中的自然殺手細胞數都顯著地降低。另外也使用RU486(糖皮質素的拮抗劑)來探討糖皮質素在誘導脾臟自然殺手細胞發生細胞凋亡所扮演的角色,結果顯示RU486並不能完全抑制酒精所誘導之自然殺手細胞發生細胞凋亡。是故應有其他的機理涉及酒精誘導脾臟自然殺手細胞發生細胞凋亡。另有實驗評估caspase-3、Bax及Bcl-2在酒精誘導脾臟之自然殺手細胞發生細胞凋亡所扮演的caspase-3的活性,然而Bax及Bcl-2的角色。研究結果顯示酒精會活化蛋白表現量則不受酒精影響。
Previous studies have shown that mouse splenic natural killer (NK) cell activity is decreased after a single dose of ethanol (EtOH) by gavage. Also, the percentage of splenic NK cells is decreased in EtOH-treated mice. This study focused on the fate of the missing splenic NK cells. EtOH can produce a stress response characterized by activation of the hypothalamic-pituitary-adrenal axis. Glucocorticoids are known to alter lymphocyte trafficking, and it is possible that NK cell numbers in the blood or sites other than the spleen may increase following EtOH administration. However, the results showed that EtOH not only decreased splenic NK cells but also decreased NK cells derived from peripheral blood, bone marrow and peritoneal cavity. These results suggest that the decreased splenic NK cells in EtOH-treated mice are not caused by migration of splenic NK cells to the other sites tested here. The missing splenic NK cells probably die from glucocorticid-induced apoptosis as a result of an EtOH-induced stress response. Administration of RU 486 (glucocorticoid antagonist) partially prevented the apoptosis of splenic NK cells induced by EtOH. Also, RU 486 only partially blocked the caspase-3 activity of splenic NK cells activated by EtOH. However, Bcl-2 and Bax protein expression in splenic NK cells were not significantly altered by EtOH. Collectively, a single dose of EtOH can globally decrease NK cell population. The decreased splenic NK cells can be explained by apoptosis induced by EtOH. The glucocorticoids play a role in the apoptotic splenic NK cells induced by EtOH. Finally, these results suggest that EtOH-induced apoptosis of splenic NK cells is regulated by caspase-3 activity but not by Bcl-2 and Bax activity.
中文摘要
英文摘要
第一章 緒論
第一節 酒精1
第二節 自然殺手細胞3
第三節 急性酒精對自然殺手細胞的影響5
第四節 糖皮質素與細胞凋亡6
第五節 急性酒精中毒的老鼠模式9
第六節 動物品種10
第七節 研究動機11
第二章 實驗方法
第一節 實驗動物12
第二節 自然殺手細胞的製備
一、脾臟細胞12
二、骨髓細胞13
三、周邊血液細胞13
四、腹腔細胞13
第三節 自然殺手細胞之純化14
第四節 自然殺手細胞活性之分析14
第五節 流式細胞儀分析自然殺手細胞數目15
第六節 Annexin V分析細胞凋亡16
第七節 7-amino-actinomycin D分析細胞凋亡16
第八節 蛋白質樣品製備及定量分析
一、蛋白質樣品製備17
二、蛋白質定量分析17
第九節 西方墨點法(Western Blot)
一、SDS PAGE的製備18
二、SDS PAGE之電泳操作及轉漬18
第十節 Caspase-3/CPP32活性分析20
第十一節 結果分析20
第三章 實驗結果
第一節 急性酒精對老鼠脾臟細胞數目的影響21
第二節 急性酒精對老鼠脾臟細胞發生細胞凋亡的影響22
第三節 急性酒精對各組織中自然殺手細胞數目及活性的影響23
第四節 急性酒精對老鼠脾臟自然殺手細胞發生細胞凋亡的影響25
第五節 Caspase-3在急性酒精誘導自然殺手細胞發生細胞凋亡之角色28
第六節 Bax在急性酒精誘導脾臟自然殺手細胞發生細胞凋亡之角色31
第七節 Bcl-2在急性酒精誘導脾臟自然殺手細胞發生細胞凋亡之角色32
第四章 討論33
第五章 參考文獻39
1.Deshapriya EB. Iwase N. Are lower legal blood alcohol limits and a combination of sanctions desirable in reducing drunken driver-involved traffic fatalities and traffic accidents? Accident Anal. Prev. 1996, 28: 721-31.
2.Hills KS. Westaby D. Alcohol and the liver. Br. J. Hosp. Med. 1997, 57: 517-21.
3.Harper C. Kril J. An introduction to alcohol-induced brain damage and its causes. Alcohol Alcohol. Supplement. 1994, 2: 237-243.
4.MacGregor RR. Alcohol and immune defense. JAMA. 1986, 256: 1474-1479.
5.Jerrells TR. & Pruett SB., Immunotoxic effects of ethanol. In J. H. Dean, M. I. Luster, A. E. Munson, & I. Kimber(Eds.), Immunotoxicol. Immunopharmacol. 1994, 323-347. New York: Raven Press.
6.Garro AJ. Espina N. & Lieber CS., Ethanol and cancer. Alcohol Health and Research World, 1992, 16: 81-86.
7.Kune GA. & Vitetta L., Alcohol consumption and the etiology of colorectal cancer: a review of the scientific evidence form 1957 to 1991. Nutr. Cancer. 1992, 18: 97-111.
8.Hayes RB. Brown LM. Schoenberg JB. Greenberg RS. Silverman D T. Schwartz A.G. Swanson G.M. Benichou J. Liff JM. Hoover RN. & Pottern LM. Alcohol use and prostate cancer risk in US blacks and whites. Am. J. Epidemiol. 1996, 143: 692-697.
9.Murata M. Takayama K. Choi BC. & Pak AW., A nested-control study on alcohol drinking, tobacco smoking, and cancer. Cancer Detection and Prevention. 1996, 20: 557-565.
10.Dosemeci M. Gokmen I. Unsal M. Hayes RB. & Blair A. Tobacco, alcohol use, and risks of laryngeal and lung cancer by subsite and histologic type in Turkey. Cancer Causes and Control. 1997, 8: 729-737
11.Smith-Warner SA. Spiegelman D. Shiaw-Shyuan Y. van den Brandt PA. Folsom AR. Goldbohm A. Graham S. Holmberg L. Howe GR. Marshall JRAB. M. Potter JD. Speizer FE. Willett WC. Wolk A. & Hunter DJ. Alcohol and Breast Cancer in Women. JAMA. 1998, 279: 535-540
12.Baron JA. SandlerRS. Haile RW. Mandel JS. Mott LA. & Greenberg ER. Floate intake, alcohol consumption, cigarette smoking, and risk of colorectal cancer. J. Natl. Cancer Inst. 1998, 90: 57-62.
13.Launoy G. Milan CH. Faivre J. Pienkowski P. Milan CI. & Gignoux M. Alcohol, tobacco and oesophageal cancer: effects of the duration of consumption, mean intake and former consumption. Br. J. Cancer. 1997, 75: 1389-1396.
14.Watson RR. Prabhala RH. Abril E. Smith TL. Changes in lymphocyte subsets and macrophage functions from high, short-term dietary ethanol in C57BL/6 mice. Life Sci. 1988, 43: 865-70.
15.Meadows GG. Blank SE. Duncan DD. Influence of ethanol consumption on natural killer cell activity in mice. Alcohol., Clin. Exp. Res. 1989, 13: 476-9.
16.Wu WJ. Wolcott RM. & Pruett SB. Ethanol decreases the nimber and activity of splenic natural killer cells in a mouse model for binge drinking. J. Pharmacol. Exp. Ther. 1994, 271: 722-729.
17.Caporaso N. Romano M. Marmo R. de Sio I. Morisco F. Minerva A. Coltorti M. Hepatitis C virus infection is an additive risk factor for development of hepatocellular carcinoma in patients with cirrhosis. J. Hepatol. 1991, 12: 367-71.
18.Room R., Measuring alcohol consumption in the United States. In L. T. Kozlowski, H. M. Annis, H. D. Cappell, F. B. Glaser, M. S. Goodstadt, Y. Israel, H. Kalant, E. M. Sellers, & E. R. Vingilis(Eds.), Research Advances in Drug and Alcohol Problems. 1990, 39-80. New York: Plenum Press.
19.Anonymous. Federal Bureau of Investigation. Uniform crime reports for the United States. 1993. U. S. Department of Justice. 1994.
20.Wieczorek WF. Miller BA. & Nochajski TH. The limited utility of BAC for identifying alcohol-related problems among DWI offenders. J. Stud. Alcohol. 1992, 53: 415-419.
21.Wechsler H. Davenport A. Dowdall G. Moeykens B. & Castillo S. Health and behavioral consequences of binge drinking in college. JAMA. 1994, 272: 1672-1677.
22.國人吸煙、喝酒、嚼檳榔及上下班使用交通工具之盛行狀況分析。行政院衛生署檢疫總所疫情資訊。中華民國八十七年。
23.Chou P. Liou MY. Lai MY. Hsiao ML. and Chang HJ. Time trend of substance use among adolescent students in Taiwan, 1991-1996. J. Formos. Med. Assoc. 1999, 98: 827-831.
24.88年1-9月台閩地區「酒後(醉)駕車」交通事故分析。交通部統計處。中華民國八十八年。
25.Trinchieri G. Biology of natural killer cells. Adv. Immunol. 1989, 47: 187-376
26.Herberman RB. Ortaldo JR. Timonen T. Reynolds CW. Djeu JY. Pestka S. Stanton J. Interferon and natural killer (NK) cells. Tex. Rep. Biol. Med. 1981-82, 41: 590-5,
27.Ritz J. Schmidt RE. Michon J. Hercend T. Schlossman SF. Characterization of functional surface structures on human natural killer cells. Adv. Immunol. 1988, 42: 181-211
28.Ritz J. The role of natural killer cells in immune surveillance [editorial]. New Engl. J. Med. 1989, 320: 1748-9.
29.Janeway CA. Natural killer cells: a primitive immune system. Nature. 1989, 341: 108.
30.Hatcher FM. Kuhn RE. Destruction of Trypanosoma cruzi by Natural killer cells. Science. 1982, 218: 295-296.
31.Emery DL. Eugui EM. Nelson RT. Tenywa T. Cell-mediated immune responses to Theileria parva (East Coast fever) during immunization and lethal infections in cattle. Immunology. 1981, 43: 323-336.
32.Oldham SB. Calmodulin: its role in calcium-mediated cellular regulation. Miner. Electrolythe Metab. 1982, 8: 1-12.
33.Franceschi C. Cossarizza A. Monti D. Ottaviani E. Cytotoxicity and immunocyte markers in cells from the freshwater snail Planorbarius corneus (L.) (Gastropoda pulmonata): implications for the evolution of natural killer cells. Eur. J. Immunol. 1991, 21: 489-93.
34.Neville ME. Grimm E. Bonavida B. Frequency determination of K cells by a single cell cytotoxic assay. J. Immunol. Methods. 1980, 36: 255-268
35.LeFever AV. Piaskowski VD. Casper JT. Truitt RL. Kinetic analysis of human IL-2 activated cytotoxic cells. Immunopharmacol. Immunotoxicol. 1991, 13: 147-68.
36.Biron CA. Byron KS. Sullivan JL. Severe herpesvirus infections in an adolescent without natural killer cells. New Engl. J. Med. 1986, 320: 1731-5.
37.Bukowski JF. Welsh RM. Inability of interferon to protect virus-infected cells against lysis by natural killer (NK) cells correlates with NK cell-mediated antiviral effects in vivo. J. Immunol. 1985, 135: 3537-41.
38.Perussia B. Tutt MM. Qiu WQ. Kuziel WA. Tucker PW. Trinchieri G. Bennett M. Ravetch JV. Kumar V. Murine natural killer cells express functional Fc gamma receptor II encoded by the Fc gamma R alpha gene. J. Exp. Med. 1989, 170: 73-86.
39.Trinchieri G. O''Brien T. Shade M. Perussia B. Phorbol esters enhance spontaneous cytotoxicity of human lymphocytes, abrogate Fc receptor expression, and inhibit antibody- dependent lymphocyte-mediated cytotoxicity. J. Immunol. 1984, 133: 1869-77.
40.Trinchieri G. Perussia B. Human natural killer cells: biologic and pathologic aspects. Lab. Invest. 1984, 50: 489-513.
41.Scott P. Trinchieri G. The role of natural killer cells in host-parasite interactions. Anesthesiology. 1995, 7: 34-40.
42.Kendall RA. Targan S. The dual effect of prostaglandin (PGE2) and ethanol on the natural killer cytolytic process: effector activation and NK-cell-target cell conjugate lytic inhibition. J. Immunol. 1980, 125: 2770-7.
43.Rice SA. Dooley JR. Mazze RI. Metabolism by rat hepatic microsomes of fluorinated ether anesthetics following ethanol consumption. Anesthesiology. 1983, 58: 237-41.
44.Mufti SI. Prabhala R. Moriguchi S. Sipes IG. Watson RR. Functional and numerical alterations induced by ethanol in the cellular immune system. Immunopharmacology. 1988, 15: 85-93.
45.Ristow SS. Starkey JR. Hass GM. Inhibition of natural killer cell activity in vitro by alcohols. Biochem. Biophys. Res. Commun. 1982, 105: 1315-1321.
46.Stacey NH. Inhibition of antibody-dependent cell-mediated cytotoxicity by ethanol. Immunopharmacology. 1984, 8: 155-61.
47.Walia AS. Lamon EW. In vitro effects of ethanol and acetaldehyde on cell-mediated cytotoxicity. Prog. Clin. Biol. Res. 1990, 325: 145-53.
48.Suthanthiran M. Solomon SD. Williams PS. Rubin AL. Novogrodsky A. Stenzel KH. Hydroxyl radical scavengers inhibit human natural killer cell activity. Nature. 1984, 307: 276-278.
49.Yeralan O. Jones JM. Effects in vitro of ethanol and acetaldehyde on natural killer activity from rats. Prog. Clin. Biol. Res. 1990, 325: 165-72.
50.Wu WJ. Pruett SB. Suppression of splenic natural killer cell activity in a mouse model for binge drinking. I. Direct effects of ethanol and its major metabolites are not primarily responsible for decreased natural killer cell activity. J. Pharmacol. Exp. Ther. 1996, 278: 1325-30.
51.Wu WJ. & Pruett SB. Involvement of catecholamines and glucocorticoids in ethanol-induced suppression of splenic natural killer cell activity in a mouse model for binge drinking. Alcohol., Clin. Exp. Res. 1997, 21: 1030-1036.
52.Irwin M. Hauger RL. Jones L. Provencio M. Britton KT. Sympathetic nervous system mediates central corticotropin-releasing factor induced suppression of natural killer cytotoxicity. J.Pharmacol. Exp. Ther. 1990, 255: 101-7.
53.Han YC. Lin TL. Pruett SB. Thymic atrophy caused by ethanol in a mouse model for binge drinking: involvement of endogenous glucocorticoids. Toxicol. Appl. Pharmacol. 1993, 123: 16-25.
54.Carson EJ. Pruett SB. Development and characterization of a binge drinking model in mice for evaluation of the immunological effects of ethanol. Alcohol., Clin. Exp. Res. 1996, 20: 132-138.
55.Redei E. Clark WR. McGivern RF. Ethanol induced alteration of immune function may involve hormones of the hypothalamic-pituitary-adrenal axis. Prog. Clin. Biol. Res. 1990, 325: 313-320
56.Nieto MA. Gonzalez A. Gambon F. Diaz-Espada F. Lopez-Rivas A. Apoptosis in human thymocytes after treatment with glucocorticoids. Clin. Exp. Immunol. 1992, 88: 341-4.
57.King KL. Cidlowski JA. Cell cycle regulation and apoptosis. Annu. Rev. Physiol. 1998, 60: 601-17.
58.Evans RM. The steroid and thyroid hormone receptor superfamily. Science. 1988, 240: 889-95.
59.Hollenberg SM. Evans RM. Multiple and cooperative trans-activation domains of the human glucocorticoid receptor. Cell. 1988, 55: 899-906.
60.Schwartzman RA. Cidlowski JA. Glucocorticoid-induced apoptosis of lymphoid cells. Int.Arch. Allergy . Immunol. 1994, 105: 347-54.
61.Chapman MS. Askew DJ. Kuscuoglu U. Miesfeld RL. Transcriptional control of steroid-regulated apoptosis in murine thymoma cells. Mol. Endocrinol. 1996, 10: 967-978.
62.Helmberg A. Auphan N. Caelles C. Karin M. Glucocorticoid-induced apoptosis of human leukemic cells is caused by the repressive function of the glucocorticoid receptor. EMBO J. 1995, 14: 452-60.
63.Rhee K. Bresnahan W. Hirai A. Hirai M. Thompson EA. c-Myc and cyclin D3 (CcnD3) genes are independent targets for glucocorticoid inhibition of lymphoid cell proliferation. Cancer Res. 1995, 55: 4188-95.
64.Thulasi R. Harbour DV. Thompson EB. Suppression of c-myc is a critical step in glucocorticoid-induced human leukemic cell lysis. J. Biol. Chem. 1993, 268: 18306-18312.
65.Thompson EB. Thulasi R. Saeed MF. Johnson BH. Glucocorticoid antagonist RU 486 reverses agonist-induced apoptosis and c-myc repression in human leukemic CEM-C7 cells. Ann. N. Y. Acad. Sci. 1995, 761: 261-75.
66.Strasser-Wozak EM. Hattmannstorfer R. Hala M. Hartmann BL. Fiegl M. Geley S. Kofler R. Splice site mutation in the glucocorticoid receptor gene causes resistance to glucocorticoid-induced apoptosis in a human acute leukemic cell line. Cancer Res. 1995, 55: 348-53 .
67.Hala M. Hartmann BL. Bock G. Geley S. Kofler R. Glucocorticoid-receptor-gene defects and resistance to glucocorticoid-induced apoptosis in human leukemic cell lines. Int. J. Cancer. 1996, 68: 663-8.
68.Ashraf J. Thompson EB. Identification of the activation-labile gene: a single point mutation in the human glucocorticoid receptor presents as two distinct receptor phenotypes. Mol. Endocrinol. 1993, 7: 631-42.
69.Powers JH. Hillmann AG. Tang DC. Harmon JM. Cloning and expression of mutant glucocorticoid receptors from glucocorticoid-sensitive and -resistant human leukemic cells. Cancer Res. 1993, 53: 4059-65.
70.Geley S. Hartmann BL. Hala M. Strasser-Wozak EM. Kapelari K. Kofler R. Resistance to glucocorticoid-induced apoptosis in human T-cell acute lymphoblastic leukemia CEM-C1 cells is due to insufficient glucocorticoid receptor expression. Cancer Res. 1996, 56: 5033-8.
71.Moreno MB. Memon SA. Zacharchuk CM. Apoptosis signaling pathways in normal T cells: differential activity of Bcl-2 and IL-1beta-converting enzyme family protease inhibitors on glucocorticoid- and Fas-mediated cytotoxicity. J. Immunol. 1996, 157: 3845-9.
72.Miyashita T. Mami U. Inoue T. Reed JC. Yamada M. Bcl-2 relieves the trans-repressive function of the glucocorticoid receptor and inhibits the activation of CPP32-like cysteine proteases. Biochem. Biophys. Res. Commun. 1997, 233: 781-7.
73.Brunet CL. Gunby RH. Benson RS. Hickman JA. Watson AJ. Brady G. Commitment to cell death measured by loss of clonogenicity is separable from the appearance of apoptotic markers. Cell Death & Differ. 1998, 5: 107-15.
74.Benson RS. Dive C. Watson AJ. Comparative effects of Bcl-2 over-expression and ZVAD.FMK treatment on dexamethasone and VP16-induced apoptosis in CEM cells. Cell Death Differ. 1998, 5: 432-9.
75.Korsmeyer SJ. Regulators of cell death. Trends Genet. 1995, 11: 101-5.
76.Adams JM. Cory S. The Bcl-2 protein family: arbiters of cell survival. Science. 1998, 281: 1322-6.
77.Gohel A. McCarthy MB. Gronowicz G. Estrogen prevents glucocorticoid-induced apoptosis in osteoblasts in vivo and in vitro. Endocrinology. 1999, 140: 5339-47.
78.Huang ST. Cidlowski JA. Glucocorticoids inhibit serum depletion-induced apoptosis in T lymphocytes expressing Bcl-2. FASEB J. 1999, 13: 467-76.
79.Sun XM. MacFarlane M. Zhuang J. Wolf BB. Green DR. Cohen GM. Distinct caspase cascades are initiated in receptor-mediated and chemical-induced apoptosis. J. Biol. Chem. 1999, 274: 5053-60.
80.Cohen GM. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326 ( Pt 1): 1-16.
81.Fukuzuka K. Edwards CK 3rd. Clare-Salzler M. Copeland EM 3rd. Moldawer LL. Mozingo DW. Glucocorticoid-induced, caspase-dependent organ apoptosis early after burn injury. Am. J. Physiol. 2000, 278: R1005-18.
82.Elgebaly SA. Kozol RA. Kreutzer DL. Alcohol and immune system: role of gastric tissue. Prog. Clin. Biol. Res. 1990, 325: 75-8.
83.Rall TW., Hypnotics and sedatives; ethanol. In A. G. Gilman, T. W. Rall, A. S. Nies, & P. Taylor(Eds.), Goodman and Gilman’s The Pharmacological Basis of Therapeutics. 1990, 370-378. New York: Pergamon Press.
84.Finn DA. Bejanian M. Jones BL. Syapin PJ. & Alkana RL. Temperature affects ethanol lethality in C57BL/6, LS, and SS mice. Pharmacol. Biochem. Behavior. 1989, 34: 375-380.
85.Andrews LS. & Snyder R., Toxic effects of solvents and vapors. In M. O. Amdur, J. Doull, & C. D. Klaassen(Eds.), Casarett and Doull’s Toxicology. 1991, 681-722. New york: Pergamon Press.
86.Tichy M. Trcka V. Roth Z. & Krivucova M. QSAR analysis and data extrapolation among mammals in a series of aliphatic alcohols. Environ. Health Perspect. 1985, 61: 321-328.
87.Freireich EJ. Gehan EA. Rall DP. Schmidt LH. & Skipper HE. Quantitative comparison of toxicity of anticancer agents in mouse, rat, hamster, dog, monkey, and man. Cancer Chemother. Rep. 1996, 50: 219-244.
88.Bryant J. DayR. Whiteside T. Herberman RB. Calculation of lytic units for the expression of cell-mediated cytoxicity. J. Immunol. Methods. 1992, 146:91-103.
89.Luster MI. Munson AE. Thomas PT. Holsapple MP. Fenters JD. White KL. Lauer LD. Germolec DR. Rosenthal GJ. Dean JH. Methods evaluation. Development of testing battery to assess chemical-induced immunotoxicity: National Toxicology Program’s quidelines for immunotoxicity evaluation in mice. Fundam. Appl. Toxicol. 1988, 10:2-19.
90.Ingrid S. Christel HU. Birgitte K. Janis VG. A rapid method for measurig apoptosis and dual-color immunofluorescence by single laser flow cytometry. J. Immunol. Methods. 1994, 170: 145-157.
91.Mann CL. Hughes FM Jr. Cidlowski JA. Delineation of the signaling pathways involved in glucocorticoid-induced and spontaneous apoptosis of rat thymocytes. Endocrinology. 2000, 141:528-38.
92.Cupps TR. and Fauci AS. Corticosteroid-mediated immunoregulation in man. Immunol. Rev. 1992, 65: 133-155.
93.Slukvin IL. and Jerrells TR. Different pathways of in vitro ethanol-induced apoptosis in thymocytes and splenic T and B lymphocytes. Immunopharmacology. 1995, 31: 43-57.
94.Ewald SJ. and Shao H. Ethanol increases apoptotic cell death of thymocytes in vitro. Alcohol., Clin. Exp. Res. 1993, 17: 359-365.
95.Singhal PC. Patel P. Nahar N. Franki N. Kapasi A. Reddy K. Shah N. Nwakoby IE. Mehrotra B. Ethanol-induced neutrophil apoptosis is mediated through nitric oxide. J. Leuk. Biol. 1999, 66: 930-6.
96.Neuman MG. Shear NH. Cameron RG. Katz G. Tiribelli C. Ethanol-induced apoptosis in vitro. Clin. Biochem. 1999, 32: 547-55.
97.Slomiany BL. Piotrowski J. Slomiany A. Chronic alcohol ingestion enchances tumor necrosis factor-alpha expression and salivary gland apoptosis. Alcohol., Clin. Exp. Res. 1997, 21: 1530-1533.
98.Gilian Z. Shi QY. Ming Y. Hui ZL. Rudura R. Anna MD., Alcohol and cytokine-inducible transcription factors. Alcohol., Clin. Exp. Res. 1996, 20: 1639-1645.
99.Kurose I. Higuchi H. Miura S. Saito H. Watanabe N. Hokari R. Hirokawa M. Takaishi M. Zeki S. Nakamura T. Ebinuma H. Kato S. Ishii H. Oxidative stress-mediated apoptosis of hepatocytes exposed to acute ethanol intoxication. Hepatology. 1997, 25: 368-378.
100.Emanuele MA. Tentler JJ. Kirsteins L. The effect of “binge” ethanol expresoure on growth hormone and prolactin gene expression and secretion. Endocrinology. 1992, 131: 2077-2082.
101.Josefesson E. Bergquist J. Ekman R. Tarkowski A. Catecholamines are synthesized by mouse lymphocytes and regulate function of these cells by induction of apoptosis. Immunol. 1996, 88: 140-6, 1996
102.Yin D. Tuthill D. Mufson RA. Shi Y. Chronic restraint stress promotes lymphocyte apoptosis by modulating CD95 expression. J. Exp. Med. 2000, 191: 1423-8.
103.Deaciuc IV. Fortunato F. D’Souza NB. Schmidt J. Lee EY. McClain CJ. Modulation of caspase-3 activity and Fas ligand mRNA expression in rat liver cells in vivo by alcohol and lipopolysaccharide. Alcohol., Clin. Exp. Res. 1999, 23: 349-356.
104.Miyashita T. Nasgao K. Krajewski S. Salvesen GS. Reed JC. Inoue T. Yamada M., Investigation of glucocorticoid-induced apoptotic pathway:processing of caspase-6 but not caspase-3. Cell Death Differ. 1998, 5: 1034-41.
105.Rieger J. Durka S. Streffer J. Dichgans J. Weller M. Gemcitabine cytokicity of human malignant glioma cell: modulation by antioxidants, Bcl-2 and Dexamethasone. Eur. J. Pharmacol. 1999, 365: 301-308.
106.Mok CL. Gil-Gomez G. William O. Coles M. Taga S. Tolaini M. Norton T. Kioussis D. Brady HJ. Bad can act as a key regulator of T cell apoptosis and T cell development. J. Exp. Med. 1999, 189: 575-586.
107.Montani MS. Tuosto L. Giliberti R. Stefanini L. Cundari E. Piccolelle E. Dexamethasone induced apoptosis in human T cell clones expressing Low levels of Bcl-2. Cell Death Differ. 1999, 6: 79-86.
108.Ghislaine D. Chantal H. Myriam V. Jacoues B. Marie-Paule D. p53, Bax and Bcl-2 in vivo Expression in the murine thymus after apoptogenic treatments. Anticancer res. 1998, 18: 3315-3522.
109.Phong TL. Holden TM. Jane EC. In situ detection and characterization of apoptotic thymocytes in human thymus: Expression of Bcl-2 in vivo does not prevent apoptosis. J. Immunol. 1995, 154: 4371-4378.
110.Se-te JH. and John AC. Glucocorticoids inhibit serum depletion-induced apoptosis in T lymphocytes expressing Bcl-2. FASB J. 1999, 13: 467-476.
111.Weimann E. Baixeras E. Zamzami N. Kelly P. Prolactin blocks glucocorticoid induced cell death by
inhibiting the disruption of the mitochondrial membrane. Leuk. Res. 1999, 23: 751-62.
112.Lotern J. and Sachs L. Regulation of bcl-2, bcl-xL and bax in the control apoptosis by hematopoietic cytokines and dexamethasone. Cell Growth Differ. 1995, 6: 647-653.
113.Chao DT. Linette GP. Boise LH. White LS. Thompson CB. Korsmeyer SJ. Bcl-xL and Bcl-2 repress a common pathway of cell death. J. Exp. Med. 1995, 182: 821-828.
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