臺灣博碩士論文加值系統

求偶行為是動物的自然本能，動物透過許多感官線索辨識同種類且合適的對象進而繁衍後代，這些自然的本能通常發生在異性之間，但在許多的物種也發現同性間的求偶行為，這其中所涉及的細胞及分子機制仍未被清楚釐清。在本研究中我們證實WIDE AWAKE (WAKE)在成年的雄果蠅神經系統中參與抑制雄果蠅同性間求偶行為的發生。wake突變所引起的雄果蠅同性間求偶行為必需同時在追求者(courter)與被追求者(courtee) wake基因功能缺陷時才會發生，當任一方是突變基因的異型合子(heterozygous)時，這種行為將被抑制發生。利用RU486誘導wake dsRNAi大量表現在神經系統中，藉以下調WAKE的表現也同樣可以看到雄果蠅同性間求偶行為的發生。有趣的是，我們也初步證實WAKE可能是透過調節GABAA receptor Resistant to Dieldrin (Rdl)的表現量來抑制雄果蠅同性間的求偶行為。這些證據顯示WAKE在神經系統中參與調節雄果蠅同性間求偶行為的發生，進一步了解WAKE在脊椎動物中對男同性戀行為的影響是一個有趣的方向。

Animals present several sensory cues to identify the same species and suitable partner for reproduction. Courtship, an instinct of animals in the nature, normally occurs in between opposite sexes. Interestingly, a lot of researches proved that courtship and sexual behaviors existed between the same sexes in many species, but the cellular or molecular mechanism is not clear. In this study, I demonstrate the existence of functional WIDE AWAKE (WAKE) and their acute requirement for inhibiting inter-male courtship in the neuronal system of adult Drosophila. I showed that hypomorphic mutants of the essential wake gene on both partners cause male-male courtship behavior, and can be rescued in either the wake heterozygous courter, or the courtee. Consistently, the knocking down of WAKE by expressing UAS-RNAiwake under an RU486-inducible pan-neuronal driver (elav-GeneSwitch) also caused inter-male courtship behavior. Interestingly, the WAKE may modulate the GABAA receptor Resistant to Dieldrin (Rdl) levels for inhibiting male-to-male courtship. These data explicitly demonstrate the involvement of wake in Drosophila inter-male courtship behavior and may lead to important advances in the understanding in vertebrates.

目次
誌謝 I
摘要 II
Abstract III
目次 IV
圖目次 V
表目次 VI
第1章　研究背景(Background) 1
第1節　果蠅模式動物 1
第2節　果蠅的求偶行為 1
第3節　wake基因 5
第2章　研究目的(Specific Aims) 14
第3章　材料與方法(Materials and Methods) 15
第1節　果蠅品系與培養條件 15
第2節　果蠅求偶行為 16
第2.1節　果蠅求偶觀測盤設計與記錄 16
第2.2節　果蠅間求偶行為分析 17
第2.3節 鏈型雄果蠅求偶行為分析 18
第3節　藥物誘導調控dsRNAi表現於果蠅成蟲階段的神經系統 18
第4節　果蠅活動力分析 18
第5節　果蠅體表碳氫化合物分析 19
第5.1節 果蠅體表碳氫化合物萃取 19
第5.2節 氣相色譜質譜分析 19
第4章　結果(Results) 21
第1節　dWAKE調控雄果蠅的同性間求偶行為 21
第2節　dWAKE在神經系統中調控雄果蠅的同性間求偶行為 24
第3節　dWAKE在神經系統中可能經由RDL調控雄果蠅同性間的求偶行為 27
第4節　dWAKE可調節雄果蠅體表碳氫化合物的組成 30
第5章　討論(Discussion) 35
參考文獻(References) 40

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