臺灣博碩士論文加值系統

小分子毒素泛指所有分子量小於 5000的毒素，此類毒素在日常生活中分佈十分廣泛，容易因誤食而危害身體健康，本研究主題為氯黴素 (Chloamphenicol; CAP) 與玉米赤黴烯酮 (Zearalenone ; ZEA)，我們期望藉由建立一套有效檢測氯黴素及玉米赤黴烯酮的方法，降低因誤食危害身體健康的機率。氯黴素為一廣效性抗生素，常用於治療動物細菌性感染，但食用過量會造成骨髓生成不良等疾病，為了避免人類誤食藥物殘留的食物，我們製備對氯黴素具有專一性的多株抗體，並且建立直接競爭型酵素連結免疫吸附分析法 (Competitive direct enzyme-linked immunosorbent assay ; cdELISA) 和非直接競爭型酵素連結免疫吸附分析法 (Competitive indirect enzyme-linked immunosorbent assay ; ciELISA)，及以奈米金粒子為標記物的快速免疫層析試紙分析法，藉由上述分析方式快速的從食品內檢測氯黴素殘留量，在cdELISA、 ciELISA及快速免疫層析試紙分析法中，偵測限制量分別為0.071、0.032 與 10 ng/ml。玉米赤黴烯酮為一種常見於穀物的黴菌毒素，具有性荷爾蒙的功能，養殖場的豬隻吃到帶有玉米赤黴烯酮的飼料會導致母豬有子宮腫脹、流產與不孕，而在公豬則會有睪丸萎縮與乳腺增大等症狀，這些症狀嚴重影響養殖豬場上的經濟效益，因此我們製備對玉米赤黴烯酮具有專一性的多株抗體，並且建立 cdELISA、 ciELISA 及快速免疫層析試紙分析法，其偵測限制量分別為 0.014、 0.086 與 1 ng/ml。研究結果顯示本實驗所製備的多株抗體可有效偵測氯黴素及玉米赤黴烯酮，並且成功建立快速且專一性高的偵測方法，可用於快速檢測食品中氯黴素及玉米赤黴烯酮。

Antibiotics and mycotoxins are both low-molecular-weight nonimmunogenic toxins, such toxins widely distributed in our daily life,which are frequently found in food and feed. This study focused on chloramphenicol (CAP) and zearalenone (ZEA), and developed an efficient method to detect the toxin. Chloramphenicol (CAP) is a prescription antibiotics commonly used to treat more serious bacterial infections on human and animal, but excessive use will lead to inhibit human bone marrow hematopoietic function. For detecting the level of CAP in foods, polyclonal antibody for CAP was produced in our current studies. A competitive direct enzyme-linked immunosorbent assay (cdELISA), a competitive indirect ELISA (ciELISA) and gold nanoparticle immunochromatographic strip were used for detecting the CAP residues in foods. The detection limit of CAP in the cdELISA, ciELISA and gold nanoparticle immunochromatographic strip were found to be 0.071, 0.032 and 10 ng/ml, respectively. Zearalenone is a mycotoxin commonly in corn and has an estrogenic and anabolic activity. Its major effects are on reproduction, including reproductive organs and function, leading to hyperestrogenism. It is dramatic intluenced on farm pigs to ingest zearalenone. Eating feed containing zearalenone, the sow will be swell uterus, abortion and infertility; the boar will be testicular atrophy and increased breast. These symptoms seriously affect the economic and ecological in farms. A cdELISA、ciELISA and gold nanoparticle immunochromatographic strip were used for detecting the ZEA residues in foods. The detection limit in the of ZEA cdELISA, ciELISA and gold nanoparticle immunochromatographic strip were found to be 0.014, 0.086 and 1 ng/ml, respectively. This immunochromatographic strip provides a rapid and sensitive detection method that can determine the level of CAP and ZEA in the food products.

目錄
(Index)
中文摘要 ………………………………………… 1
英文摘要 ………………………………………… 2
緒論 ……………………………………………… 3
材料與方法 ……………………………………… 10
結果 …………………………………………….. 24
討論 ……………………………………………... 30
圖表 ……………………………………………… 40
參考文獻 ………………………………………… 56
附錄 ……………………………………………… 61

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