臺灣博碩士論文加值系統

本論文主要探討微毛細管電泳晶片之微機電製程技術及其臨床應用，製程上分別選用了Glass、PC (Poly-carbonate)、PDMS (Polydimethyl-siloxane)三種高透光性及高生物相容性之材料，因此製程可分為下列兩種不同方式：
(1) 微細加工之玻璃晶片
此研究運用微影製程及微細加工技術，在玻璃基材上製作出微流通道，最後運用接合技術，將已有樣品儲存槽之上玻璃基板接合。
(2) 翻模技術之塑膠晶片
此晶片利用微影製程、薄膜沉積及電鑄等技術，製作出微通道模具，再利用熱壓及模造技術製作出微通道底板，最後再與高透光性之軟性基材接合。
在臨床實驗上則以血清中糖化血紅素的分離為主，以測試微毛細管電泳晶片之性能。本研究之微毛細管電泳晶片有簡易製作及快速分析之優點，具有臨床及醫學檢測之潛能。

In this research, the MEMS fabrication technologies and clinical applications of micro capillary electrophoresis (CE) chip were studied. Using three kinds of materials with high penetrability and high bio-compatibility such as Glass, Poly-carbonate (PC), and Polydimethyl-siloxane (PDMS), two different fabrication processes were developed:
(1) Glass CE chip using micro-machining technology
Lithography, micro-machining and binding technologies are main steps.
(2) Polymer CE chip using replication technology
Lithography, micro-machining and deposition technologies were used to fabricate the master of CE channel. Hot-embossing and soft-lithography were then utilized to manufacture the CE chip, followed by binding with soft-substrate of high-penetrability.
The clinical experiments were also performed for the separation of HbA1C. This CE chip has many merits and potential for the clinical and medical applications.

第一章　緒論
1.1 緣起…………….....…………………………………1
1.2 研究背景及動機 .………………..…………………3
1.3 文獻回顧……………………………………………….4
1.4 研究目標……………………………………….…..…5
第二章　毛細管電泳原理
2.1毛細管電泳原理……….......…..…………….……….7
2.1 毛細管電泳的分類……..…..........………….……….10
第三章　實驗方法
3.1 毛細管電泳晶片製作技術 ……….………………….16
3.1.1 微影製程技術的應用……..………………….16
3.1.2 熱壓成型技術…………………………………23
3.2 毛細管電泳晶片實驗方法及實驗步驟………….…..24
3.2.1玻璃毛細管電泳晶片製作步驟……..….……26
3.2.2高分子材料之毛細管電泳片製作步驟………27
第四章　結果與討論
4.1 玻璃毛細管電泳晶片製作…..…................…...29
4.2 高分子材料之毛細管電泳片製作…............……36
4.2.1 PC材質毛細管電泳晶片製作…...................…36
4.2.2 PDMS材質毛細管電泳晶片製作.....................42
4.3 臨床實驗….....................…...............……….42
4.3.1 臨床實驗背景………....................…...……….42
4.3.2 臨床實驗目標………....................…...……….44
4.3.3 臨床實驗架構………....................…...……….44
4.3.4 臨床實驗結果….........….....….…............…….45
第五章　結論與未來展望
5.1 結論............……........……..………...………...49
5.2 未來展望…………...........................………………….50
參考文獻……………………………..………………………...…….51

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