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研究生:陳亭亭
研究生(外文):Ting-ting Chen
論文名稱:血藤(MucunamacrocarpaWALLICH)葉部及莖部化學成分之研究
論文名稱(外文):Studies on the Chemical Constituents in the Leaves and Stems of Mucuna macrocarpa WALLICH
指導教授:張永勳張永勳引用關係何禮剛何禮剛引用關係
指導教授(外文):Yuan-Shiun ChangLi-Kang Ho
學位類別:碩士
校院名稱:中國醫藥大學
系所名稱:中國藥學研究所
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:2005
畢業學年度:93
語文別:中文
論文頁數:120
中文關鍵詞:豆科血藤成分
外文關鍵詞:Mucuna macrocarpaconstituents
相關次數:
  • 被引用被引用:4
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血藤Mucuna macrocarpa WALLICH為豆科(Leguminosae)之台灣常見植物,具有補血活血、通經活絡、手腳麻木及清肺潤燥等功能。在台灣民間曾用血藤來治療糖尿病。
在DPPH自由基清除能力之試驗中,血藤藤莖之甲醇粗抽物、水層、正丁醇、乙酸乙酯層及水不溶層具有顯著的抗氧化活性。
在成分研究中,從血藤之各分層分離純化,一共分離出七個化合
物,經光譜分析鑑定其結構分別為-sitosterol和stigmasterol混合物(H-1), betulin (C-1), medicarpin (E-1), sucrose (B-1), D-pinitol (B-2), -sitosterol-3-O--D-glucoside(N-1), tetracosanoic acid 2,3-dihydroxy- propyl ester(N-2)。
藉由HPLC定量分析血藤之genistein含量,以了解血藤之成分與其功效的相關性。定量結果genistein在血藤中含量百分比為0.001%,所以推測血藤顯示之活性可能為其含有之多種黃酮類及其他種類之成分所共同作用帶來的效果。
希望以植物血藤的化學成分研究,來確定其成分種類,進而瞭解血藤在醫療上的功效,及民間用途使用的正確性,以提高台灣本土藥用植物之利用價值。
Mucuna macrocarpa WALLICH is a native plant of Taiwan belonging to the Leguminosae family. It is a folk medicine being used for blood circulation, activating meridians and collaterals, moisten dryness, release stagnated lung-energy. This plant was also used in Taiwan folk medicine for diabetes mellitus.

In the assay for DPPH scavenging effect, the fractions of crude extract, water layer, n-butanol layer, ethyl acetate layer and precipitates showed good free radical scavenging effects.

In the phytochemical study, five compounds were isolated from M. macrocarpa and were identified as the mixture of -sitosterol and stigmasterol (H-1), betulin (C-1), medicarpin (E-1), sucrose (B-1), D-pinitol (B-2), -sitosterol-3-O--D-glucoside (N-1) and tetracosanoic acid 2,3-dihydroxypropyl ester (N-2)。

We also carried out the HPLC quantitative analysis of genistein in M. macrocarpa , in order to find out the composition of M. macrocarpa and its dependence of the efficiency. Content percentage of genistein was 0.001% in M. macrocarpa. This may suggest that the activities of M. macrocarpa was resulted together from many kinds of flavonoids and other constituents.

Since this plant is rather common in Taiwan, further study and investigation of its active constituents may prove its potential biological activities and medicinal values.
目錄 I
表目錄 III
圖目錄 IV
中文摘要 VII
英文摘要 VIII
第一章 緒 言1
第二章 總 論
第一節 血藤之本草學考察 3
第二節 血藤之藥用植物學考察 5
第三節 血藤(M. macrocarpa)之相關文獻考察 9
第四節 血藤屬(Mucuna)之植物成分及藥理文獻整理 12
第三章 實驗部分
第一節 實驗試劑與儀器 27
第二節 實驗藥材來源及其抽提與分離 31
第三節 血藤之自由基清除能力評估 37
第四節 血藤中Genistein定量分析 39
第四章 結 果
第一節 血藤各成分之物理性質及光譜數據 41
第二節 血藤之自由基清除能力結果 56
第三節 血藤中Genistein定量分析結果 58
第五章 討 論
第一節 血藤化學成分之結構鑑定 61
第二節 血藤之自由基清除能力討論 80
第三節 血藤中Genistein定量分析討論 81
第六章 結 論 82
參考文獻 84
圖譜 103
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