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研究生:王凱玟
研究生(外文):Kai-Wen Wang
論文名稱:樟芝人參複方之抗疲勞功效評估
論文名稱(外文):Evaluation of anti-fatigue properties of Antrodia camphorata and ginseng drink
指導教授:黃啟彰黃啟彰引用關係鄭世忠鄭世忠引用關係
指導教授(外文):Chi-Chang HuangShih-Chung Cheng
口試委員:廖辰中
口試委員(外文):Chen-Chung Liao
口試日期:2015-06-01
學位類別:碩士
校院名稱:國立體育大學
系所名稱:運動與健康科學學院
學門:民生學門
學類:運動科技學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:中文
論文頁數:75
中文關鍵詞:牛樟芝人參抗疲勞乳酸血氨尿素氮肌酸激酶
外文關鍵詞:Antrodia camphorataPanax ginsenganti-fatiguelactateammoniaBUNCK
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目的:探討樟芝人蔘滋補液對於改善身體疲勞以及增強運動表現之生物功效。方法:本研究採用動物模式評估此複方對於運動所引起之疲勞及其相關生理、生化指標進行系統性評估。連續補充不同劑量複方(80、240與480毫克/每公斤體重/每日)四週後,依序進行運動表現與抗疲勞相關測試,項目包括:前肢抓力、5%負重游泳力竭時間以及運動挑戰後血液中乳酸、血氨、血糖、尿素氮濃度以及肌肉損傷指標肌酸激酶活性之變化。以SAS統計軟體進行單因子變異數分析,並以Duncan's test檢驗不同組之間是否存在顯著差異性,並以p<0.05表示具有統計上意義。結果:實驗數據顯示,在運動表現方面,補充樟芝人蔘滋補液的中與高劑量組具有明顯提升運動耐力時間表現,且在前肢抓力上有補充複方組均顯著高於控制組 (p<0.05)。此外,根據趨勢統計分析(trend analysis)顯示,樟芝人蔘滋補液除了對於提升耐力與肌力上具有劑量效應之外,也能夠有效降低與疲勞相關之生化指標包括:血氨、血乳酸、尿素氮以及肌酸激酶上升程度。結論:本項研究成果除了證實此兩種中草藥複方萃取物具有抗疲勞與提升運動表現功效之外,也能提供相關保健食品研發廠廠商針對相關的萃取物進行產品開發的依據,此一生物檢測平台亦可提供國內企業有意從事開發或驗證具有抗疲勞或運動增補劑潛力產品的科學依據。



關鍵字:牛樟芝、人參、抗疲勞、運動表現、乳酸、血氨、尿素氮、肌酸
Antrodia camphorata and Panax ginseng are well-known medicinal plants in Taiwan folk and traditional Chinese medicine, which have been reported for multifunctional bioactivities such as anti-tumor, anti-inflammation, immunoregulation, anti-oxidation and anti-fatigue. However, there is limited evidence that a fixed combination formula of these two plants extract is effective for the exercise improvement or anti-fatigue. The purpose of this study was to evaluate the potential beneficial effects of the mix formulation of these two herbal medicines (AG formulation) on fatigue and ergogenic functions following physiological challenge. Male ICR mice from four groups (n = 10 per group) were orally administered AG formulation for 4 weeks at 0, 80, 240 and 480 mg/kg/day, which were respectively designated the Vehicle, AG-1X, AG-3X and AG-6X groups. The anti-fatigue activity and exercise performance were evaluated using forelimb grip strength, exhaustive swimming time, and levels of serum lactate, ammonia, glucose, and creatine kinase (CK) after a 15-min swimming exercise. The exhaustive swimming time of the 3X and 6X AG group was significantly longer than that of Vehicle group and the AG supplementation group also significantly higher than that of Vehicle group. AG supplementation also produced dose-dependent decreases in serum lactate, BUN, ammonia, and CK activity after the 15-min swimming test. Therefore, the AG complex could be a potential formulation with an anti-fatigue pharmacological effect. This formulation could be further developed to commercial products for people who pursue of health promotion and this platform also could provide industrial to evaluate their products with specific bio-functional activity.


Keywords: Antrodia camphorata, Panax ginseng, anti-fatigue, lactate, ammonia, BUN, creatine kinase

目 錄
中文摘要 Ⅰ
英文摘要 Ⅱ
謝誌 Ⅲ
目錄 Ⅳ
圖目錄 Ⅵ
表目錄 Ⅶ
第壹章 緒論 1
第一節 研究背景 1
第二節 研究目的 2
第貳章 文獻探討 3
第一節 運動與疲勞之相關研究 3
第二節 樟芝介紹及其生理活性相關研究 7
第三節 人參介紹及其生理活性相關研究 11
第四節 薑黃素介紹及其生理活性相關研究 16
第五節 牛磺酸介紹及其生理活性相關研究 21
第六節 文獻小節 25
第參章 材料與方法 26
第一節 實驗測試樣品 26
第二節 實驗動物之飼養及分組 28
第三節 實驗內容與方法 29
第四節 血液與組織樣本之收集及前處理 34
第五節 統計分析 36
第肆章 結果與討論 37
第一節 體重與飲食記錄 37
第二節 肌力與耐力之運動表現 39
第三節 運動挑戰後之疲勞相關血液生化指標變化 43
第四節 肝臟及肌肉組織中肝醣含量變化 53
第伍章 結論 56
參考文獻 57
附件一、國立體育大學動物實驗審查同意書 75


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