臺灣博碩士論文加值系統

近年來在果蠅腦科學研究上，多以人工的方式來進行實驗，本論文改良舊有多重刺激源睡眠監控平台，輔以自動化程式控制，將刺激源模組化，並使用氣味及濕度做為主要刺激源，用非接觸的方式降低果蠅可能因外力而造成的傷害，在夜晚給予刺激源干擾來剝奪果蠅的睡眠。在實驗分析方面，則用紅外線攝影機錄下果蠅晝夜活動情形，配合影像分析做後處理，來觀察受刺激源干擾後的睡眠情形。實驗使用四種果蠅喜惡性較明顯之氣味，分別為香蕉油、蘋果醋、OCT、BA；利用常溫下50%~60%以及80%~90%相對濕度，來探討環境中的濕度對於果蠅睡眠行為的影響。未來期望此創新的多重刺激源睡眠監控平台，能在果蠅睡眠研究方面，提供更多的行為研究模型，同時可藉由多重刺激源模組功能，使日後科學家在果蠅神經及基因方面，做更加深入的了解。

In recent years, neurogenetics research of drosophila experiment is more needed. In this thesis, novel sleep monitoring platform which is capable of installing multiple stimulation modules and automatic monitoring of sleep is developed. The stimulation modules are controlled by LabVIEW programs.
This study gave the odorant and humidity stimuli to disturb the sleep of flies in the night. The physical damage is minimal using the non-contact method. The sleep behavior video was recorded by Infrared camera to provide drosophila sleep data.
The novel sleep monitoring platform provides a new neurogenetics research tool in drosophila sleep. In the future, scientists are able to develop effective medicine to help people who suffers from insomnia.

目錄
第1章 緒論 1
1.1 前言 1
1.2 研究動機及目的 4
第2章 文獻回顧 7
2.1 果蠅 7
2.2 溫濕度 8
2.3 嗅覺(氣味) 12
2.4 風 18
2.5 聽覺(聲音) 19
2.6 光源 21
第3章 平台設計理念及整合 22
3.1 舊有睡眠監控平台 22
3.2 改良型睡眠監控平台設計 28
3.2.1 平台尺寸及密閉性設計 29
3.2.2 氣味刺激源改良及程式設計 31
3.2.3 濕度模組 33
第4章 睡眠實驗規劃 34
4.1 果蠅睡眠實驗 34
4.1.1 實驗目的 34
4.1.2 實驗方法 36
第5章 實驗結果與討論 38
5.1 環境變異數因子控制 38
5.2 不同氣味對於果蠅睡眠之影響 39
5.2.1 氣味實驗－對照組 40
5.2.2 氣味實驗－實驗組 40
5.3 不同濕度對於果蠅睡眠之影響 49
5.3.1 濕度實驗－對照組 49
5.3.2 濕度實驗－實驗組 49
5.4 實驗結果討論 53
5.4.1 對照組實驗 57
5.4.2 氣味實驗組 65
5.4.3 濕度實驗組 73
5.4.4 睡眠參數指標之探討 81
5.4.5 其他可能原因 81
第6章 結論與未來展望 83
6.1 結論 83
6.2 未來展望 84
參考文獻 85

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