臺灣博碩士論文加值系統

本論文主要探討不同佐劑及抗原呈獻細胞對naive T細胞分化的影響.首先,我們使用不同的佐劑(包括Alum及CFA)致敏小鼠,同時追蹤小鼠抗OVA專一性抗體IgG及IgE的表現.為了了解抗原呈獻細胞本身是否可因產生不同的細胞激素而影響T細胞的分化,我們分析不同部位的抗原呈獻細胞(包括腹腔巨噬細胞,腹腔B細胞,及皮下Langerhans cells)產生IL-10,IL-12之能力繼之我們分別以皮下注射法及腹腔注射法注入同樣的抗原,欲研究不同的路徑注入抗原與不同的抗OVA抗體的產生是否有關.我們的結果顯示佐劑本身會影響免疫反應的型態:以CFA為佐劑致敏之小鼠有較高的抗OVA IgG2a抗體,同時其脾臟細胞細胞激素IFN-g/IL-4之比值亦較高.進一步地,我們轉移以CFA為佐劑致敏之小鼠的腹水細胞,發現CFA可以抑制以Alum為佐劑致敏之小鼠免疫反應.由於發現以CFA為佐劑致敏之小鼠的腹水細胞有較高量的gd T細胞,故猜測在此抑制作用中,gd T細胞可能扮演相當重要的角色.由不同部位的抗原呈獻細胞分泌細胞激素IL-10,IL-12的結果來看,B細胞明顯有大量IL-10表現,巨噬細胞可表現IL-10及IL-12,Langerhans cells只有IL-12表現.然而與以皮下注射及腹腔注射注入同樣的抗原之結果比較,兩者產生之抗OVA專一性抗體不是十分明顯.總括而言,我們的結果顯示以CFA為佐劑致敏之小鼠有較高的抗OVA IgG2a,同時其脾臟細胞細胞激素IFN-g/IL-4之比值亦較高.不同的抗原呈獻細胞產生的細胞激素例如IL-10和IL-12不同,至於其對不同抗體之產生扮演什麼角色有待進一步的研究.

The purpose of this study is to investigate the effect of diffe-rent adjuvants and antigen-presenting cells (APCs) on T cell de-velopment. Firstly, we used aluminum hydroxide (Alum) or compl-ete Freund''s adjuvant (CFA) as adjuvant to sensitize mice and followed up the production of IgG and IgE anti-OVA antibodies.
In order to realize whether the cytokines secreted by APCs can influence T cell development or not, we analyzed the level of IL-10 and IL-12 secretion of different kinds of APCs including peritoneal macrophages, peritoneal B cells, and Langerhans cells. Then, we set up an animal model by using i.p. injection and s.c. injection to study the correlation of different routes of antigen antibodies. Our data showed that the adjuvants themsel- ves could modify the pattern of immune response: (1). IgG2a ant- i-OVA antibody was higher in mice sensitized with OVA-CFA; (2).
The ratio of IFN-g/IL-4 induced was higher in mice sensitized
with OVA-CFA. Furthermore, the immune response of mice sensiti-
zed with OVA-Alum was inhibited by the transferred ascitic cells
from CFA-immunized mice. Because of abundant gd T cells in the
peritoneal cells of CFA-immunized mice, it is speculated the gd
T cells may play an important role in this inhibition. From the
cytokine secreted by APCs, we found that B cells expressed IL-10
predominantly, macrophages expressed both IL-10 and IL-12, and
Langerhans cells expressed IL-12 only. However, compared to the
result of different routes of antigen administration, the corre-
lation with certain isotype of anti-OVA antibody was not so obv-
ious. In general, our data suggested higher IgG2a and the ratio
of IFN-g/IL-4 was noted in mice sensitized with OVA-CFA. Cytok-
ines level such as IL-10 and IL-12 were different among differe-
nt kinds of APC and their role in production of different isoty-
pes of antibodies needs to be further elucidated.