臺灣博碩士論文加值系統

本研究將咖啡含在膠原蛋白複合軟膏，以活體內試驗，利用全厚度皮膚切除之動物傷口模型，觀察其對傷口癒合之影響，評估結合咖啡之膠原蛋白複材做為軟膏以加速傷口癒合之可行性。在咖啡的選擇方面，從5種不同產地的咖啡豆研磨成咖啡粉並透過咖啡機沖煮，讓咖啡粉所含的咖啡成分釋出於水，再熬煮濃縮後加水定量製成濃度不同的咖啡萃取液。並且經過研究實驗後，選擇在抗氧化之活性試驗中，以活性較強之義大利咖啡豆進行本次的研究開發。接著，比較咖啡和膠原蛋白(collagen)的抗氧化能力，再進行活體實驗，在體內實驗中動物共分成八組，分組情形如下，第一組：為優碘軟膏，第二組：只給予膠原蛋白軟膏，第三組：咖啡軟膏，第四組：咖啡因軟膏，第五組：10％膠原蛋白加1000 ppm咖啡因軟膏，第六組：10％膠原蛋白加1000 ppm咖啡軟膏，第七組：5％膠原蛋白加500 ppm咖啡因軟膏及第八組：5％膠原蛋白加500 ppm咖啡軟膏，進行傷口癒合之探討，並進行病理組織分析，以皮膚檢體做組織染色，觀察傷口癒合情形與上皮細胞移動情形。本研究發現咖啡能藉由增強纖維黏蛋白(fibronectin)、膠原蛋白及轉化生長因子(transforming growth factor)-β的表現促進傷口的癒合。

In the present study, we added coffee to the collagen ointment to evaluate the influence in wound healing with the animal model of full thickness skin wound. In coffee choices, we selected five kinds of coffee beams from different origins to grind and brew by the coffee machine. Then, coffee components from coffee powder was released into water and boiled. After condensation, we added water to make coffee extracts in different concentrations. We chose the Italian coffee beam with better antioxidative activity to carry out the study in antioxidative experiments. Then, we investigated antioxidative activities of coffee and collagen and perform the study in vivo. The experimental animals were divided into eight groups : 1. Povidone iodine ointment, 2. Collagen ointment, 3. Coffee ointment, 4. Caffeine ointment, 5. 10% collagen combined with 1000 ppm caffeine ointment, 6. 10% collagen combined with 1000 ppm coffee ointment, 7. 5% collagen combined with 500 ppm caffeine ointment, 8. 5% collagen combined with 500 ppm coffee ointment. We also observe wound healing and epithelium migration by pathological analysis and H&E stain. Therefore, we found that coffee could promote wound healing via fibronectin、collagen and transforming growth factor -β.

中文摘要…………………………………………………………………..........Ⅰ
英文摘要…………………………………………………………………..........Ⅱ
誌謝………………………………………………………………………..........Ⅲ
目錄………………………………………………………………………..........Ⅳ
表目錄……………………………………………………………………..........Ⅵ
圖目錄……………………………………………………………………..........Ⅶ
第一章 前言………………………………………………………………..........1
第二章 總論………………………………………………………………..........5
第一節 皮膚與傷口…………………………………………………..........5
第二節 自由基與傷口癒合………………………………………………13
第三節 影響因素…………………………………………………………24
第四節 研究之獨特性……………………………………………………29
第五節 研究之關鍵性……………………………………………………29
第六節 研究之重要性……………………………………………………30
第三章 材料與方法……………………………………………………………31
第一節 實驗動物…………………………………………………………31
第二節 儀器設備…………………………………………………………31
第三節 試劑與藥品 ………………………………………………..........31
第四節 實驗方法 ......................................................................................33
(一) 、咖啡萃取方法……………………………………………...........33
(二) 、HPLC檢測caffeine,chlorogenic acid實驗……………………..33
(三) 、抗氧化實驗……………………………………………………….35
(四) 、軟膏之製作……………………………………………………….35
(五) 、各種軟膏之過敏性試驗………………………………………….38
(六) 、各種軟膏原料之刺激性試驗…………………………………….39
(七) 、傷口癒合之動物實驗…………………………………………….39
(八) 、Wistar Rats新生皮膚組織之固定………………………….........41
(九) 、Hematoxylin and eosin染色 (H&E染色)…………………........41
(十) 、免疫組織化學染色………………………………………….........42
(十一)、統計學分析……..….....…………………………………........43
第四章 結果………………………………………………………………........44
第五章 討論………………………………………………………………........48
第六章 結論………………………………………………………………........50
第七章 參考文獻……………………………………………………………....67

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