臺灣博碩士論文加值系統

E-cadherin蛋白存在所有表皮細胞上，主要功能是形成細胞與細胞間的黏附作用以維持組織間的完整形態。當細胞上的E-cadherin蛋白表現異常，細胞的生長便失去contact inhibition的特性，因此造成細胞的增生與分化不良，因此當上皮細胞的E-cadherin失去其應有的功能時細胞便開始癌化。本論文的目的在探討E-cadherin在腫瘤進行過程中所扮演的角色，因此分析不同FIGO staging之侵入性子宮頸癌檢體中，E-cadherin基因之表現情形。
共收集77例不同FIGO staging之侵入性子宮頸癌Ia（N=15），Ib （N=19），Ib meta（N=13），IIa（N=17），IIb（N=13）病理石臘包埋檢體，以進行組織免疫化學染色分析，並收集18例新鮮冷凍之外科手術切除之子宮頸癌Ib檢體及其對照之正常組織，以進行半定量之RT-PCR分析，所得結果經統計分析得知，E-cadherin的表現強度與腫瘤轉移與否及病人之癒後情形無關，但與腫瘤組織的大小呈反比關係（p < 0.001）。值得注意的是細胞膜上是否有表現E-cadherin蛋白可能才是腫瘤是否轉移之重要指標（p =0.001，< 0.005）。

Epithelial adhesion molecule, E-cadherin plays important role in maintaining structural integrity of epithelial tissue. Altered expression of E-cadherin might result in the loss of contact inhibition growth property, abnormal cell growth and differentiation, hence the oncogenic transformation.
To explore the role of E-cadherin in the tumor progression, we have investigate the expression pattern of E-cadherin in invasive cervical carcinomas. 77 paraffin embedded specimen with FIGO staging Ia（N=15）, Ib（N=19）, Ib meta（N=13） IIa（N=17）, IIb（N=13）were included in immunohistochemical study, 18 surgical removed tumor tissues of Ib stage and its normal counter parts were used for semi-quantitative RT-PCR analysis. The results indicated that no significant correlation between E-cadherin expression level and tumor metasis, patient prognosis. However, E-cadherin immunostainimg intensity was significanty inverse correlated with tumor size（p < 0.001）. Notably, loss of membranes E-cadherin expression might be a significant factor that correlated with tumor metasis（p = 0.001,< 0.005）.

中文摘要… 2
英文摘要… 3
文獻探討… 4
材料與方法…8
實驗結果 19
討論……23
參考文獻 27
表………32
圖………43

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