臺灣博碩士論文加值系統

細本山葡萄Vitis thunbergii SIEB. & ZUCC.為葡萄科(Vitaceae)植物，又名小本山葡萄、小葉山葡萄、山葡萄等別名，其根莖具有清熱、利尿、袪濕、解毒、消腫止血、生肌之效。主治風濕關節痛、風濕痺痛、腳氣腫、肝炎黃疸、痢疾、淋病、小便不利、腫毒、跌打損傷，為台灣固有之民間用藥。
本研究自細本山葡萄根部之甲醇粗抽物分離出的成分有lupeol(H-1)、ß-sitosterol(H-2)、l-dotriacontanol(H-3)、friedelin (C-1)、betulin(C-2)、 vanillic acid(EA-1)、caffeic acid(EA-2)等成分。由於過去對於細本山葡萄研究甚少，因此希望藉由細本山葡萄植物的化學成分研究，來確定其成分種類，進而了解細本山葡萄在醫療上的功效。而本研究實驗所分離出成分之藥理活性，則有待進一步之探討與確認。

Vitis thunbergii SIEB. & ZUCC. is a native plant of Taiwan belonging to the Vitaceae family. It is a common folk medicine being used for rheumatism, hepatitis, jaundice, dysentery and blennorrhagia etc..
From its methanolic extracts, seven compounds were isolated： lupeol(H-1), ß-sitosterol(H-2), l-dotriacontanol(H-3), friedelin(C-1), betulin(C-2), vanillic acid(EA-1) and caffeic acid(EA-2). Their structures were determined by spectroscopic methods.
In the past, few reseaches were done on Vitis thunbergii SIEB. & ZUCC. Througt the chemical studies of the ingredients, the medical use of this plant can be understood. However, the pharmacological activities of the compounds isolated needs further investigation.

目 錄
目 錄 I
表目錄 II
圖目錄 IV
中文摘要 VII
英文摘要 VIII
第一章 緒言 1
第二章 總論 3
第一節 細本山葡萄之本草學考察 3
第二節 細本山葡萄之藥用植物學考察 8
第三節 細本山葡萄之Napralert文獻考察 17
第四節 葡萄屬植物之Napralert文獻考察 19
第五節 山葡萄植物之Napralert文獻考察 20
第三章 實驗部分 26
第一節 實驗試劑與儀器 26
第二節 實驗藥材來源及其抽提與分離 30
第四章 結 果 33
第五章 討 論 40
第六章 結 論 59
參考文獻…… 61
圖譜.........71



表目錄
Table 2-1：細本山葡萄植物形態特徵表 13
Table 2-2：Ethnomedical information on Vitis thunbergii 18
Table 2-3：Biological activities for extracts of Vitis thunbergii 20
Table 2-4：Presence of compounds in Vitis flexuosa 21
Table 2-5：Ethnomedical information on Ampelopsis brevipedunculata 22
Table 2-6：Biological activities for extracts of Ampelopsis brevipedunculata 23
Table2-7：Presence of compounds in Ampelopsis brevipedunculata 25
Table 5-1：化合物H-1碳譜數據與文獻lupeol碳譜數據比對表 48
Table 5-2：化合物H-2碳譜數據與文獻ß-sitosterol碳譜數據比對表 51
Table 5-4：化合物C-1碳譜數據與文獻friedelin碳譜數據比對表 57
Table 5-5：化合物C-2碳譜數據與文獻betulin碳譜數據比對表 61


圖目錄
Fig. 2-1：歷代本草所繪蘡薁(細本山葡萄)圖 6
Fig. 2-2：蘡薁(細本山葡萄)之本草系統圖 8
Fig. 2-3：細本山葡萄(Vitis thunbergii SIEB. & ZUCC.)植物圖 13
Fig. 2-4：細本山葡萄(Vitis thunbergii SIEB. & ZUCC.)植物型態特徵圖 14
Fig. 3-1：細本山葡萄根之抽提流程圖 38
Chart 1：The IR (KBr) spectrum of lupeo(H-1)…………………………….71
Chart 2：The MS (EI) spectrum of lupeol (H-1)……………………………..71
Chart 3：The 1H-NMR(CDCl3) spectrum of lupeol (H-1)………………...72
Chart 4：The 1H-NMR(CDCl3) spectrum of lupeol (H-1)…………...…73
Chart 5：The 13C-NMR (CDCl3) spectrum of lupeol (H-1)……………….73
Chart 6：The 13C-NMR (CDCl3) spectrum of lupeol (H-1)………………...74
Chart 7：The DEPT (π/4, 2π/4,3π/4) spectrum of lupeol (H-1)…………. 75
Chart 8：The IR (KBr) spectrum of ß-sitosterol (H-2)…………………….76
Chart 9：The MS (EI) spectrum of ß-sitosterol (H-2)………………………76
Chart 10：The 1H-NMR (CDCl3) spectrum of ß-sitosterol (H-2)…………77
Chart 11：The 1H-NMR (CDCl3) spectrum of ß-sitosterol (H-2)………...78
Chart 12：The 13C-NMR (CDCl3) spectrum of ß-sitosterol (H-2)………..78
Chart 13：The 13C-NMR (CDCl3) spectrum of ß-sitosterol (H-2)………..79
Chart 14：The DEPT (π/4, 2π/4,3π/4) spectrum of ß-sitosterol (H-2)……80
Chart 15：The IR (KBr) spectrum of l-dotriacontanol (H-3)………………81
Chart 16：The MS (EI) spectrum of l-dotriacontanol (H-3)……………….81
Chart 17：The 1H-NMR (CDCl3) spectrum of l-dotriacontanol (H-3)…..82
Chart 18：The 1H-NMR (CDCl3) spectrum of l-dotriacontanol (H-3)…..83
Chart 19：The 13C-NMR (CDCl3) spectrum of l-dotriacontanol (H-3)…..83
Chart 20：The 13C-NMR (CDCl3) spectrum of l-dotriacontanol (H-3)….84
Chart 21：The DEPT (π/4, 2π/4,3π/4) spectrum of l-dotriacontanol (H-3)….85
Chart 22：The IR (KBr) spectrum of friedelin (C-1)………………………..86
Chart 23：The MS (EI) spectrum of friedelin (C-1)………………………..86
Chart 24：The 1H-NMR(CDCl3) spectrum of friedelin (C-1)…………..….87
Chart 25：The 1H-NMR(CDCl3) spectrum of friedelin (C-1)…………….88
Chart 26：The 13C-NMR (CDCl3) spectrum of friedelin (C-1)………89
Chart 27：The 13C-NMR (CDCl3) spectrum of friedelin (C-1)……….90
Chart 28：The DEPT (π/4, 2π/4,3π/4) spectrum of friedelin (C-1)………90
Chart 29：The DEPT (π/4, 2π/4,3π/4) spectrum of friedelin (C-1)………91
Chart 30：The IR (KBr) spectrum of betulin (C-2)…………………………91
Chart 31：The 1H-NMR(CDCl3) spectrum of betulin (C-2)………………92
Chart 32：The 1H-NMR(CDCl3) spectrum of betulin (C-2)……………….93
Chart 33：The 1H-NMR(CDCl3) spectrum of betulin (C-2)……………….94
Chart 34：The The 13C-NMR (CDCl3) spectrum of betulin (C-2)……….94
Chart 35：The The 13C-NMR (CDCl3) spectrum of betulin (C-2)……….95
Chart 36：The DEPT (π/4, 2π/4,3π/4) spectrum of betulin (C-2)…………96
Chart 37：The IR (KBr) spectrum of vanillic acid (EA-1)……………...97
Chart 38：The MS (EI) spectrum of vanillic acid (EA-1)………………….97
Chart 39：The 1H-NMR (DMSO) spectrum of vanillic acid (EA-1)…….98
Chart 40：The 1H-NMR (DMSO) spectrum of vanillic acid (EA-1)….....99
Chart 41：The 13C-NMR (DMSO) spectrum of vanillic acid (EA-1)…...99
Chart 42：The 13C-NMR (DMSO) spectrum of vanillic acid (EA-1)….100
Chart 43：The DEPT (π/4, 2π/4,3π/4) spectrum of vanillic acid (EA-1).100
Chart 44：The IR (KBr) spectrum of caffeic acid(EA-2)…………………101
Chart 45：The MS (EI) spectrum of caffeic acid(EA-2)………………….101
Chart 46：The 1H-NMR (DMSO) spectrum of caffeic acid(EA-2)……..102
Chart 47：The 1H-NMR (DMSO) spectrum of caffeic acid(EA-2)……..103
Chart 48：The 13C-NMR (DMSO) spectrum of caffeic acid(EA-2)…….104
Chart 49：The DEPT (π/4, 2π/4,3π/4) spectrum of caffeic acid(EA-2)…105

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