臺灣博碩士論文加值系統

柚皮素(Naringenin)，一種被發現在柑橘類水果中的類黃酮，具有顯著多樣的藥理學性質。在本研究中，我們設計有關柚皮素對於止痛與抗發炎作用的相關研究。在大鼠與小鼠身上進行熱板測試、醋酸誘導扭動與甩尾測定來評估柚皮素的抗傷害作用。另外，透過角叉菜誘導的大鼠足腫脹測試來進行柚皮素抗發炎作用。我們發現口服柚皮素(100與200 mg/kg)的老鼠顯著延遲了對於熱板試驗所產生的熱刺激的反應時間，並且甩尾測定與醋酸誘導扭體次數也相對減少。另外，柚皮素在角叉菜誘導爪腫脹的測試中顯著抑制大鼠足腫脹，發揮抗發炎作用。我們的研究結果支持柚皮素具有止痛和抗發炎作用的治療潛力，並且可以進一步用於疼痛與發炎相關疾病的藥物開發。

Naringenin, a flavonoid found in citrus fruits, has notably diverse pharmacological properties. In the present study, we designed to investigate the anti-nociceptive and anti-inflammatory effects of the naringenin.
The anti-nociceptive effect was evaluated using hot-plate, acetic acid-induced writhing, and tail-flick assays in mice and rats. In addition, the anti-inflammatory effect was examined by carrageenan induced paw edema test in rats.
Naringenin (100, 200 mg/kg, oral administration) significantly delayed the reaction time of mice to the thermal stimulation generated with the hot-plate, and tail-flick unit and reduced acetic acid-induced writhing response in mice. In addition, naringenin significantly repressed the paw edema induced by carrageenan in rats, exerting its anti-inflammatory effect.
Our results support that the naringenin has therapeutic potential with anti-nociceptive and anti-inflammatory properties and can be further exploitable for drug development of pain and inflammatory related diseases.

中文摘要 i
英文摘要 ii
目次 iii
圖表目次 iv
第一章 前言 1
ㄧ、 簡介 1
二、 發炎(Inflammation) 2
三、 疼痛(Pain) 4
四、 柚皮苷(Naringin) 7
五、 柚皮素(Naringenin) 7
六、 研究目的 7
第二章 材料及方法 8
ㄧ、 研究對象 8
二、 實驗方法 8
第三章 結果 12
ㄧ、 柚皮素對小鼠熱傷害的影響 12
二、 柚皮素對醋酸引起的小鼠扭體傷害的影響 12
三、 柚皮素對大鼠甩尾測試的影響 13
四、 柚皮素對角叉菜膠誘導大鼠足腫脹的影響 13
第四章 討論 14
一、 探討柚皮素的作用 14
二、 引發痛覺試驗與止痛藥關係 14
三、 柚皮素與抗發炎反應的關係 14
第五章 參考資料 16
圖表目次 iv
表一、 柚皮素對角叉菜膠誘發的足腫脹試驗的影響 22
圖一、 柚皮素的化學結構 23
圖二、 柚皮素對熱板試驗測試確定的疼痛閾值之影響 24
圖三、 柚皮素對醋酸導致小鼠扭體反應的影響 25
圖四、 柚皮素對甩尾測試的影響 26

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