臺灣博碩士論文加值系統

本試驗旨在評估：(一)新開發Suigen PCV2疫苗在田間使用之效益，(二)應用PCV2/M.hyopneumoniae混合雙價疫苗免疫對降低PCV2誘發PRDC困擾豬場之效益。試驗一於一大型有輕度PCVAD困擾豬場中進行，除了解Suigen PCV2疫苗兩劑免疫(3及6週)對PCVAD之控制效益外，亦同步比較二劑免疫及一劑免疫(3週)間是否有差異，及以一劑Suigen PCV2疫苗免疫與目前已在台灣上市二種PCV2品牌疫苗(品牌A及B)進行比較。在試驗期間分別統計豬隻消瘦死亡及體重，且採集不同生長階段之血液進行病毒載量及抗體分析。結果顯示在一劑或兩劑Suigen PCV2疫苗免疫後，與對照組相比，在肉豬階段可見有較佳的生長性能及較低的死亡率，並能有效降低PCV2病毒血症及病毒載量，且在免疫後能有效激發PCV2特異性抗體的揚昇。在一次免疫情形下與其他兩個不同A及B廠牌商用PCV2疫苗相比，亦無差異性。在病理學監控中，經Suigen PCV2疫苗免疫後豬隻並無PCVAD的病例出現。顯示該新開發疫苗即使在免疫一劑情形下，也能有效控制PCVAD。
試驗二係在台灣有嚴重PMWS及PRDC困擾之養豬場進行田間試驗，比較牧場在施打PCV2/M.hyopneumoniae雙價疫苗免疫前後豬隻死亡率、咳嗽指數、屠體豬隻肺部病變的差異及PCV2病毒血症的變化，以評估雙價混合疫苗之控制效益。結果顯示，在免疫後之豬群其咳嗽指數、屠體SEP樣病變均明顯低於未免疫豬群，在12週齡PCV2病毒血症也在免疫後，其PCV2病毒載量均顯著逐漸下降，在肥前豬隻之死亡率亦在施打疫苗後逐漸下降。以上結果顯示此PCV2/M.hyopneumoniae混合雙價疫苗可有效控制PCV2所引發PRDC之困擾並降低肥前肉豬之死亡率。

The aim of this study was to evaluate the efficacy of the novel Suigen PCV2 vaccine and assess the protective efficiency of M. hyopneumoniae/PCV2 combined vaccine in a PCV2 associated disease (PCVAD) condemned farm. The first trial was conducted in a farm with mild PCVAD history. The control efficacy of 2 doses of Suigen PCV2 vaccinations (3 and 6 weeks age) including the percentage of wasting pigs, mortality, and mean daily weight gain were evaluated dependent on the clinical record. The PCV2 viral load and antibody titer in blood of experimental pigs between 3 and 18 weeks of age were assayed by quantitative real-time PCR and ELISA. Those results were also compared to 1 dose of Suigen PCV2 vaccination and other two commercial vaccines. The results displayed whether double or single vaccinations of Suigen PCV2 vaccine not only show greater growth performance and lower mortality, but also less virus loads in pre-finishers than control group. Moreover, single vaccination of Suigen PCV2 vaccine also displayed similar efficacy to other two PCV2 commercial vaccines. Additionally, there was no pig died of PCVAD in the Suigen PCV2 vaccinated pigs. The results show even single vaccination of the Suigen PCV2 vaccine can effectively control PCVAD.
The trial 2 was conducted in a farm with PMWS and PRDC history using a vaccination program of M. hyopneumoniae/PCV2 combined vaccine on 3 weeks old piglets. The cough index of pre-finishing pigs and swine enzootic pneumonia (SEP)-like lesion score of slaughter pigs were evaluated. In addition, monitoring PCV2 viremia of growing pigs with or without vaccination and mortality were undertaken. The results show that the cough index and the severity of SEP-like lesion score were significantly decreased in the vaccinated population. Pigs with high virus loads (>106 copies/mL) were gradually decreased after a long-term PCV2 vaccination. Besides, the mortality of pre-finishing pigs were obviously decreased after the implementation of the combined vaccinated program. These results suggest that the PCV2/M. hyopneumoniae combined vaccine can efficiently control PCV2 associated PRDC.

中文摘要 -----------------------------------------------------------------------------I
英文摘要 -----------------------------------------------------------------------------II
目錄 -----------------------------------------------------------------------------------III
表次 ------------------------------------------------------------------------------------V
圖次 ------------------------------------------------------------------------------------VI
第一章 文獻探討 --------------------------------------------------------------------1
第一節 豬第二型環狀病毒(PCV2) -----------------------------------------1
1.1.1 歷史背景與流行病學 -------------------------------------------1
1.1.2 豬第二型環狀病毒之特性--------------------------------------2
1.1.3 豬第二型環狀病毒之傳播途徑--------------------------------4
第二節 豬環狀病毒相關疾病(PCVAD)------------------------------------4
1.2.1 PMWS臨床症狀與病理變--------------------------------------6
1.2.2 其他PCV2相關症候群與疾病--------------------------------7
1.2.3 PCVAD之診斷----------------------------------------------------9
1.2.4 PCVAD相關致病因子-------------------------------------------11
1.2.5 PCV2與宿主免疫反應及疫苗開發---------------------------12
1.2.6 PCVAD之控制策略----------------------------------------------15
第三節 黴漿菌(Mycoplasma)---- --------------------------------------------19
2.3.1 1.3.1 黴漿菌簡介與特性------------------------------------------------19
2.3.2 1.3.2 豬肺炎黴漿菌------------------------------------------------------19
2.3.3 1.3.3 豬黴漿菌肺炎臨床症狀與病理變化---------------------------20
1.3.4 豬黴漿菌肺炎與豬呼吸道疾病複合症之關連性------------21
1.3.5 豬黴漿菌肺炎之鑑定診斷---------------------------------------22
1.3.6 豬黴漿菌肺炎預防與控制---------------------------------------23
第二章 新型豬第二型環狀病毒疫苗田間使用效益評估---------------------26
第一節 前言 -------------------------------------------------------------------26
第二節 實驗目的與設計 ----------------------------------------------------27
第三節 材料與方法 ----------------------------------------------------------28
第四節 結果---------------------------------------------------------------------33
第五節 討論 -------------------------------------------------------------------39
第三章豬肺炎黴漿菌及豬第二型環狀病毒混合雙價疫苗使用效益評估-52
第一節 前言 -------------------------------------------------------------------52
第二節 實驗目的與設計 ----------------------------------------------------54
第三節 材料與方法 ----------------------------------------------------------55
第四節 結果---------------------------------------------------------------------58
第五節 討論 -------------------------------------------------------------------61
第四章 參考文獻 --------------------------------------------------------------------73

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